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The map label for this gene is tuf1

Identifier: 74316409

GI number: 74316409

Start: 419487

End: 420677

Strand: Direct

Name: tuf1

Synonym: Tbd_0391

Alternate gene names: 74316409

Gene position: 419487-420677 (Clockwise)

Preceding gene: 74316408

Following gene: 74316410

Centisome position: 14.42

GC content: 62.3

Gene sequence:

>1191_bases
ATGGCTAAGGAAAAATTCGAGCGGACCAAACCGCACGTAAACGTAGGCACGATTGGTCACGTTGACCACGGCAAGACCAC
CTTGACCGCGGCGATCACGACCATTCTGTCGAAGAAATTCGGTGGTGCGGCGAAGAAGTACGACGAGATCGATTCCTCGC
CTGAAGAGAAGGCGCGCGGCATCACCATCAACACCGCCCACGTCGAGTACGAGACCGCCAGCCGTCACTACGCCCACGTC
GACTGCCCGGGCCACGCCGACTACGTCAAGAACATGATCACCGGCGCTGCCCAGATGGACGGCGCCATTCTTGTCGTCTC
CTCTGCCGACGGTCCCATGCCCCAGACCCGCGAGCACATCCTGCTTGCCCGTCAGGTCGGCGTGCCCTACATCATCGTCT
ACATGAACAAGGCCGACATGGTCGACGACGCCGAGCTCCTCGAGCTCGTCGAAATGGAAGTGCGCGAACTGCTGTCCAAG
TACGACTTCCCTGGCGACGACACCCCCATCATCGTCGGTAGCGCCCTGAAGGCCCTCGAAGGCGACCAGAGCGACATCGG
CGAACCCAGCATCATCAAGCTCGCCGAAGCGCTCGACACCTACATCCCCGAGCCCGAGCGCGCCATCGACAAGCCCTTCC
TCATGCCCGTCGAAGACGTCTTCTCCATCTCCGGTCGCGGCACCGTCGTCACCGGTCGTGTCGAGCGCGGCGTCATCAAG
GTCGGCGAAGAAATCGAGATCGTCGGCATCACCCCGACCGTCAAGACCACCTGCACCGGCGTCGAGATGTTCAGGAAGCT
CCTCGACCAGGGACAGGCTGGCGACAACGTCGGCGTGCTGCTGCGCGGCACCAAGCGTGAAGAAGTCCAGCGCGGCCAGG
TTCTGGCCAAGCCCGGCTCGATCAAGCCGCACACCAAGTTCTCCGCCGAGATCTATGTGTTGAGCAAGGACGAAGGCGGT
CGTCATACCCCCTTCTTCAACGGCTACCGTCCGCAGTTCTATTTCCGCACCACCGACGTCACCGGCTCGATCGAACTGCC
GGCCGGCACCGAGATGGTCATGCCTGGCGACAACGTCAGCATCAAGGTCTCGCTGATCCAGCCGATCGCGATGGACGAAG
GCCTGCGTTTCGCCATCCGCGAAGGCGGCCGCACCGTCGGTGCCGGCGTCGTTGCCAAGATCGAAGAGTAA

Upstream 100 bases:

>100_bases
TCGGCGCACCGGGTTTTGGGTGCCGTGAAGCACGGCGTACCGGGTATTGGTTGTCGAAGTCGACGCATCGTTACTTGTGC
AAATTTGAGGGGTACTTGAC

Downstream 100 bases:

>100_bases
AGGCGGCAAGCTGCAAGGCCGGGTTGGGCGTCCATGCATGCGCCCGGCCCGGCCTTAAGTAATACAGGCCAATAGCTCAA
TTGGTAGAGCGTCGGTCTCC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGAAKKYDEIDSSPEEKARGITINTAHVEYETASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIVGSALKALEGDQSDIGEPSIIKLAEALDTYIPEPERAIDKPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGITPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVQRGQVLAKPGSIKPHTKFSAEIYVLSKDEGG
RHTPFFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVSIKVSLIQPIAMDEGLRFAIREGGRTVGAGVVAKIEE

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGAAKKYDEIDSSPEEKARGITINTAHVEYETASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIVGSALKALEGDQSDIGEPSIIKLAEALDTYIPEPERAIDKPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGITPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVQRGQVLAKPGSIKPHTKFSAEIYVLSKDEGG
RHTPFFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVSIKVSLIQPIAMDEGLRFAIREGGRTVGAGVVAKIEE
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGAAKKYDEIDSSPEEKARGITINTAHVEYETASRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHILLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIVGSALKALEGDQSDIGEPSIIKLAEALDTYIPEPERAIDKPFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGITPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVQRGQVLAKPGSIKPHTKFSAEIYVLSKDEGGR
HTPFFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVSIKVSLIQPIAMDEGLRFAIREGGRTVGAGVVAKIEE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=390, Percent_Identity=59.2307692307692, Blast_Score=503, Evalue=1e-143,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=30.6122448979592, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=28.9823008849558, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.3333333333333, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI194018520, Length=334, Percent_Identity=30.5389221556886, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI194018522, Length=334, Percent_Identity=30.5389221556886, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI194097354, Length=334, Percent_Identity=30.5389221556886, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=31.1728395061728, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI54607086, Length=346, Percent_Identity=28.0346820809249, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI4503507, Length=260, Percent_Identity=28.0769230769231, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=82.48730964467, Blast_Score=656, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=82.48730964467, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=334, Percent_Identity=29.940119760479, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=33.0543933054393, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789108, Length=313, Percent_Identity=28.7539936102236, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=54.2713567839196, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI25141371, Length=389, Percent_Identity=46.0154241645244, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI17552884, Length=459, Percent_Identity=30.2832244008715, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=459, Percent_Identity=30.2832244008715, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=439, Percent_Identity=28.246013667426, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=26.4976958525346, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=431, Percent_Identity=26.6821345707657, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.3274336283186, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=29.2358803986711, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=37.012987012987, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=439, Percent_Identity=25.5125284738041, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.8481012658228, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.9090909090909, Blast_Score=541, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.561797752809, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.561797752809, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6322937, Length=460, Percent_Identity=26.0869565217391, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=30.3571428571429, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=270, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.7310924369748, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281363316, Length=389, Percent_Identity=57.5835475578406, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=389, Percent_Identity=57.5835475578406, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=52.0725388601036, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI45553807, Length=457, Percent_Identity=28.4463894967177, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45553816, Length=457, Percent_Identity=28.4463894967177, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24651721, Length=457, Percent_Identity=28.4463894967177, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17864154, Length=457, Percent_Identity=28.4463894967177, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24652838, Length=457, Percent_Identity=28.6652078774617, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17137572, Length=457, Percent_Identity=28.6652078774617, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI45550900, Length=448, Percent_Identity=28.7946428571429, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.1639344262295, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.8850574712644, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24647046, Length=270, Percent_Identity=27.4074074074074, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24647048, Length=270, Percent_Identity=27.4074074074074, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_THIDA (Q3SLQ1)

Other databases:

- EMBL:   CP000116
- EMBL:   CP000116
- RefSeq:   YP_314149.1
- RefSeq:   YP_314161.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q3SLQ1
- SMR:   Q3SLQ1
- STRING:   Q3SLQ1
- GeneID:   3671657
- GeneID:   3671670
- GenomeReviews:   CP000116_GR
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0391
- KEGG:   tbd:Tbd_0403
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- PhylomeDB:   Q3SLQ1
- BioCyc:   TDEN292415:TBD_0391-MONOMER
- BioCyc:   TDEN292415:TBD_0403-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 43080; Mature: 42949

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGAAKKYDEIDSSPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCC
ITINTAHVEYETASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIVGSALKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDQSDIGEPSIIKLAEALDTYIPEPERAIDKPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGITPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVQRGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECCCC
IKPHTKFSAEIYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCCE
IKVSLIQPIAMDEGLRFAIREGGRTVGAGVVAKIEE
EEEEEECCCHHCCCCEEEECCCCCEECCCEEEEECC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGAAKKYDEIDSSPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCC
ITINTAHVEYETASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSSADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVYMNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIVGSALKALE
EEEHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDQSDIGEPSIIKLAEALDTYIPEPERAIDKPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGITPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREEVQRGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCEEECCCC
IKPHTKFSAEIYVLSKDEGGRHTPFFNGYRPQFYFRTTDVTGSIELPAGTEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEECCCCCE
IKVSLIQPIAMDEGLRFAIREGGRTVGAGVVAKIEE
EEEEEECCCHHCCCCEEEECCCCCEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA