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The map label for this gene is rpoH [H]

Identifier: 74316363

GI number: 74316363

Start: 363361

End: 364206

Strand: Reverse

Name: rpoH [H]

Synonym: Tbd_0345

Alternate gene names: 74316363

Gene position: 364206-363361 (Counterclockwise)

Preceding gene: 74316364

Following gene: 74316349

Centisome position: 12.52

GC content: 64.18

Gene sequence:

>846_bases
ATGACGCAGACAATGTCTTTACCGATTCCCGCCGCCTACAGTCTGGACAGCTACATCCAGACCGTGAGCCACTATCCGAT
GCTCAGCCTCGAGGAAGAGCAGAAGCTGGCGCGCGCGTGGCATGACGAACAAGACCTTGAAGCGGCGCGCCGGCTGGTGT
TGTCGCACCTGCGCGTCGTGGTGAGCATCGCTCGGCACTATCTGGGCTACGGGCTGCCGCACGCGGACCTGATCCAGGAA
GGCAACGTCGGCCTGATGAAGGCGGTCAAGCGCTTCGATCCGGACCGCGGCGTGCGGCTCGTGTCCTTCGCGCTGCACTG
GATCCGCGCTGAAATCCACGAATACGTGCTGAAGAACTACCGAATGGTCAAGCTCGCGACGACCAAGGCCCAGCGCAAGC
TCTTTTTCAACCTGCGCAGCCTCAAGCAGTCGCTCAACGCGCTGACCGTGCAGCAGGCCGACGCGATGGCGCAGGAACTC
AACGTCAGCCGCAAGGACGTGCTCGAGATGGAAACGCGGCTCTCGGGCCATGACGTCTCGATCGATCCGATGGTCGACGA
CGGCGAGGACGACTACAGCCCGATCGCCTATCTCGCGAGCGCGGAAGAGAACCCCGCCCAGCGCCTCGAGCGCGAGCAGA
CCGAACGACTGCGCGAAAGCGGCCTCACGAGCGCACTCGAAGAACTCGACGAGCGCAGCCGCCACATCATCCAGGCGCGC
TGGCTCAGCGAAGGCGACGTCGCGACGCTGCATGAACTCGCGGCGCAATACGGCGTCTCGGCCGAACGCATCCGCCAGAT
CGAAGCGCGCGCGCTGCAGAAGATGCGTGCGTCGATCCCTGCCTGA

Upstream 100 bases:

>100_bases
ATGAGATGGAGGAGGCCTATTACCGAGATGCGGGAACCCGGGCCCGCATTAGCACTCCCAATGCGCGAGTGCTAAAATTC
GCCGCAAGGAGGTCACACGC

Downstream 100 bases:

>100_bases
ACGGGAGGTTCTGCCAACAAAAAGCCCCGCCGATGCGGGGCTTTTTGTTGGCAGAAGGCGCGTTCAGAAGAACGATGCGA
TCACCACCGAGAGGATGCTG

Product: sigma-70 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTQTMSLPIPAAYSLDSYIQTVSHYPMLSLEEEQKLARAWHDEQDLEAARRLVLSHLRVVVSIARHYLGYGLPHADLIQE
GNVGLMKAVKRFDPDRGVRLVSFALHWIRAEIHEYVLKNYRMVKLATTKAQRKLFFNLRSLKQSLNALTVQQADAMAQEL
NVSRKDVLEMETRLSGHDVSIDPMVDDGEDDYSPIAYLASAEENPAQRLEREQTERLRESGLTSALEELDERSRHIIQAR
WLSEGDVATLHELAAQYGVSAERIRQIEARALQKMRASIPA

Sequences:

>Translated_281_residues
MTQTMSLPIPAAYSLDSYIQTVSHYPMLSLEEEQKLARAWHDEQDLEAARRLVLSHLRVVVSIARHYLGYGLPHADLIQE
GNVGLMKAVKRFDPDRGVRLVSFALHWIRAEIHEYVLKNYRMVKLATTKAQRKLFFNLRSLKQSLNALTVQQADAMAQEL
NVSRKDVLEMETRLSGHDVSIDPMVDDGEDDYSPIAYLASAEENPAQRLEREQTERLRESGLTSALEELDERSRHIIQAR
WLSEGDVATLHELAAQYGVSAERIRQIEARALQKMRASIPA
>Mature_280_residues
TQTMSLPIPAAYSLDSYIQTVSHYPMLSLEEEQKLARAWHDEQDLEAARRLVLSHLRVVVSIARHYLGYGLPHADLIQEG
NVGLMKAVKRFDPDRGVRLVSFALHWIRAEIHEYVLKNYRMVKLATTKAQRKLFFNLRSLKQSLNALTVQQADAMAQELN
VSRKDVLEMETRLSGHDVSIDPMVDDGEDDYSPIAYLASAEENPAQRLEREQTERLRESGLTSALEELDERSRHIIQARW
LSEGDVATLHELAAQYGVSAERIRQIEARALQKMRASIPA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=292, Percent_Identity=53.4246575342466, Blast_Score=298, Evalue=2e-82,
Organism=Escherichia coli, GI1789098, Length=262, Percent_Identity=32.0610687022901, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789448, Length=230, Percent_Identity=30.8695652173913, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32044; Mature: 31913

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTMSLPIPAAYSLDSYIQTVSHYPMLSLEEEQKLARAWHDEQDLEAARRLVLSHLRVV
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VSIARHYLGYGLPHADLIQEGNVGLMKAVKRFDPDRGVRLVSFALHWIRAEIHEYVLKNY
HHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RMVKLATTKAQRKLFFNLRSLKQSLNALTVQQADAMAQELNVSRKDVLEMETRLSGHDVS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCE
IDPMVDDGEDDYSPIAYLASAEENPAQRLEREQTERLRESGLTSALEELDERSRHIIQAR
ECCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSEGDVATLHELAAQYGVSAERIRQIEARALQKMRASIPA
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQTMSLPIPAAYSLDSYIQTVSHYPMLSLEEEQKLARAWHDEQDLEAARRLVLSHLRVV
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VSIARHYLGYGLPHADLIQEGNVGLMKAVKRFDPDRGVRLVSFALHWIRAEIHEYVLKNY
HHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RMVKLATTKAQRKLFFNLRSLKQSLNALTVQQADAMAQELNVSRKDVLEMETRLSGHDVS
EEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCE
IDPMVDDGEDDYSPIAYLASAEENPAQRLEREQTERLRESGLTSALEELDERSRHIIQAR
ECCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLSEGDVATLHELAAQYGVSAERIRQIEARALQKMRASIPA
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]