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The map label for this gene is lipB

Identifier: 74316287

GI number: 74316287

Start: 289858

End: 290514

Strand: Direct

Name: lipB

Synonym: Tbd_0269

Alternate gene names: 74316287

Gene position: 289858-290514 (Clockwise)

Preceding gene: 74316286

Following gene: 74316288

Centisome position: 9.96

GC content: 69.56

Gene sequence:

>657_bases
GTGAACGGGGCGGCGCCGATCATTCCGGACGTTCGGGTGGAGCCGGACCCCAGCCTCGTCGTGCGCCAACTCGGCCGGGT
CGCCTACGAGCCGACCTGGCGGGCGATGCAGGCCTTCGCGGCACAGCGTTCGGCGGGCACGGCGGACGAGCTGTGGCTGC
TCGAGCATCCGCCGGTCTACACGCTCGGGCAGGCGGGCAAGCGCGAGCATCTGATTGCCGCGACCGACATTCCCGTCGTC
CCGATCGACCGCGGCGGCCAGATCACCTATCACGGTCCCGGACAGGTCGTCGCCTACGTGCTGGTCGACCTGCGGCGGCG
TGGCTACGGCATCCGCGAACTCGTCGCGCGCCTGGAGCAGGCGGTGATCGACCTGCTCGCGGCGTCGAACGTCGAGGCGA
CGCGGCGCGCCGGCGCGCCTGGCGTCTACGTCGACGGCGCCAAGATCGCCGCGCTCGGCCTGCGCGTGAAGCACGGCTGC
ACCTACCATGGCCTCGCGTTCAATGTCGACATGGACCTCGAGCCGTTCGCCGCAATCAACCCCTGTGGCTATCCCGGCAT
GGCGGTCACGCAATGCCGCGATCTCGGCCTGAATTGGTCTGTCGAGCAAACGGAGCGGGCGCTCACCGACGCACTGCAGC
GCGCGATCTATTCTTGA

Upstream 100 bases:

>100_bases
ATTACCTCTCGGTCACCTGCGTCGTACGCGCCACCTCGAAGGCGCAGCTCGACGCCCTGTACCGCGAGATCACCGCCCAC
CCCTGGGTCAAGATGGCGCT

Downstream 100 bases:

>100_bases
CCGATTTGGTCGACTATTAGTAGTCTATGGACTTCTAATGGAGTGATGCATGAGCACCGACACCCTGCCGTCCGACCCCA
AACCGCTGATGCGCTCGATC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MNGAAPIIPDVRVEPDPSLVVRQLGRVAYEPTWRAMQAFAAQRSAGTADELWLLEHPPVYTLGQAGKREHLIAATDIPVV
PIDRGGQITYHGPGQVVAYVLVDLRRRGYGIRELVARLEQAVIDLLAASNVEATRRAGAPGVYVDGAKIAALGLRVKHGC
TYHGLAFNVDMDLEPFAAINPCGYPGMAVTQCRDLGLNWSVEQTERALTDALQRAIYS

Sequences:

>Translated_218_residues
MNGAAPIIPDVRVEPDPSLVVRQLGRVAYEPTWRAMQAFAAQRSAGTADELWLLEHPPVYTLGQAGKREHLIAATDIPVV
PIDRGGQITYHGPGQVVAYVLVDLRRRGYGIRELVARLEQAVIDLLAASNVEATRRAGAPGVYVDGAKIAALGLRVKHGC
TYHGLAFNVDMDLEPFAAINPCGYPGMAVTQCRDLGLNWSVEQTERALTDALQRAIYS
>Mature_218_residues
MNGAAPIIPDVRVEPDPSLVVRQLGRVAYEPTWRAMQAFAAQRSAGTADELWLLEHPPVYTLGQAGKREHLIAATDIPVV
PIDRGGQITYHGPGQVVAYVLVDLRRRGYGIRELVARLEQAVIDLLAASNVEATRRAGAPGVYVDGAKIAALGLRVKHGC
TYHGLAFNVDMDLEPFAAINPCGYPGMAVTQCRDLGLNWSVEQTERALTDALQRAIYS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=205, Percent_Identity=35.1219512195122, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI87081767, Length=199, Percent_Identity=52.7638190954774, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323268, Length=168, Percent_Identity=31.547619047619, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355483, Length=220, Percent_Identity=31.3636363636364, Blast_Score=91, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_THIDA (Q3SM28)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314027.1
- ProteinModelPortal:   Q3SM28
- SMR:   Q3SM28
- STRING:   Q3SM28
- GeneID:   3671411
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0269
- NMPDR:   fig|292415.3.peg.376
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q3SM28
- BioCyc:   TDEN292415:TBD_0269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23638; Mature: 23638

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAAPIIPDVRVEPDPSLVVRQLGRVAYEPTWRAMQAFAAQRSAGTADELWLLEHPPVY
CCCCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECCCCEE
TLGQAGKREHLIAATDIPVVPIDRGGQITYHGPGQVVAYVLVDLRRRGYGIRELVARLEQ
ECCCCCCCCEEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
AVIDLLAASNVEATRRAGAPGVYVDGAKIAALGLRVKHGCTYHGLAFNVDMDLEPFAAIN
HHHHHHHHCCCHHHHHCCCCCEEECCCEEEEEEEECCCCCEECCEEEEECCCCCCCCCCC
PCGYPGMAVTQCRDLGLNWSVEQTERALTDALQRAIYS
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNGAAPIIPDVRVEPDPSLVVRQLGRVAYEPTWRAMQAFAAQRSAGTADELWLLEHPPVY
CCCCCCCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHEEEECCCCEE
TLGQAGKREHLIAATDIPVVPIDRGGQITYHGPGQVVAYVLVDLRRRGYGIRELVARLEQ
ECCCCCCCCEEEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHH
AVIDLLAASNVEATRRAGAPGVYVDGAKIAALGLRVKHGCTYHGLAFNVDMDLEPFAAIN
HHHHHHHHCCCHHHHHCCCCCEEECCCEEEEEEEECCCCCEECCEEEEECCCCCCCCCCC
PCGYPGMAVTQCRDLGLNWSVEQTERALTDALQRAIYS
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA