Click here to switch to the map view.

The map label for this gene is dat [H]

Identifier: 74316285

GI number: 74316285

Start: 288750

End: 289598

Strand: Direct

Name: dat [H]

Synonym: Tbd_0267

Alternate gene names: 74316285

Gene position: 288750-289598 (Clockwise)

Preceding gene: 74316284

Following gene: 74316286

Centisome position: 9.92

GC content: 67.14

Gene sequence:

>849_bases
ATGAGCGTCTATCTAAACGGCCGCTTCCAGCCGCTGGCCGAGGCGACGATCCCCGTGCTCGACCGCGGCTTCGTTTTCGG
CGACGGTGTCTACGAACTGGTGCCGGTGTATTCGCGCAAGCCCTTCCGGCTCGACGCGCATCTGACGCGCCTGCAGCACA
GCCTCGACGGCATCCGTCTGAGCAACCCGCACACCCCGACGCAGTGGCGGGACCTGATCCAGCATCTGATCGCCGAGCAG
GACTTCGACGACCAATCGGTCTACATCCAGGTGACGCGGGGAACCGCGCCGCGCGACCATGCCTTCCCCGTGGGCGTGCC
GCCGACCGTGTTCATGTTCGCGCAGGCCCTCGTCACCGCGACACCTGCGCAGAAGGCGGTGGGGGTCTGCGCGGTCAGCG
CGGTCGACAACCGCTGGCTGCGCTGCGACATCAAGGCGATTTCGCTGTTGCCGAACATCCTTCTACGCCAGCAGGCGACC
GATGCCGGCTGCGCGGAAACCGTGATGTTCCGCGACGGTTTTCTGACCGAGGGCGCGGCGAGCAATATCTTCGTCGTGAA
AGACGGCGTGCTGCTGACGCCGCCCTCGTCGAACCTGATGCTCACCGGGGTGACCCACGACGTCGTGCTCGAACTCGCCG
CGACGCTCGGCGTGCCGGTCGAGATTCGCGCGATCGCCGAAGCCGAGGTGCGCCGCGCCGACGAGCTGTGGATGACATCG
TCGACCAAGGAAATCATGCCGATCGTGATGCTCGACGGCGCGCCGGTCGGCGGCGGGGCGCCCGGGCCGCTGGCGCAGAA
ATTCGACGCCGCCTACGCCGCGTTCAAACGCGAGGTCATGCGCGCATGA

Upstream 100 bases:

>100_bases
GCAAGCCGCTCGGCAACCGTCCGCTGGTCGCACTGCACGACGTCGCCGTTGCCGGCATCTTCGGGCGCGGCTGGGATTCG
CTGCGTCTGCTCTTCGACCA

Downstream 100 bases:

>100_bases
GCGAAGAAGAGACCCTGCTTCGCTTCCCGACCGACTTCCCGATCAAGATCATGGGCGAGCGGCGCGACGATTTCGCCCAG
GTCATGGTCGAACTCGTGCG

Product: D-alanine transminase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSVYLNGRFQPLAEATIPVLDRGFVFGDGVYELVPVYSRKPFRLDAHLTRLQHSLDGIRLSNPHTPTQWRDLIQHLIAEQ
DFDDQSVYIQVTRGTAPRDHAFPVGVPPTVFMFAQALVTATPAQKAVGVCAVSAVDNRWLRCDIKAISLLPNILLRQQAT
DAGCAETVMFRDGFLTEGAASNIFVVKDGVLLTPPSSNLMLTGVTHDVVLELAATLGVPVEIRAIAEAEVRRADELWMTS
STKEIMPIVMLDGAPVGGGAPGPLAQKFDAAYAAFKREVMRA

Sequences:

>Translated_282_residues
MSVYLNGRFQPLAEATIPVLDRGFVFGDGVYELVPVYSRKPFRLDAHLTRLQHSLDGIRLSNPHTPTQWRDLIQHLIAEQ
DFDDQSVYIQVTRGTAPRDHAFPVGVPPTVFMFAQALVTATPAQKAVGVCAVSAVDNRWLRCDIKAISLLPNILLRQQAT
DAGCAETVMFRDGFLTEGAASNIFVVKDGVLLTPPSSNLMLTGVTHDVVLELAATLGVPVEIRAIAEAEVRRADELWMTS
STKEIMPIVMLDGAPVGGGAPGPLAQKFDAAYAAFKREVMRA
>Mature_281_residues
SVYLNGRFQPLAEATIPVLDRGFVFGDGVYELVPVYSRKPFRLDAHLTRLQHSLDGIRLSNPHTPTQWRDLIQHLIAEQD
FDDQSVYIQVTRGTAPRDHAFPVGVPPTVFMFAQALVTATPAQKAVGVCAVSAVDNRWLRCDIKAISLLPNILLRQQATD
AGCAETVMFRDGFLTEGAASNIFVVKDGVLLTPPSSNLMLTGVTHDVVLELAATLGVPVEIRAIAEAEVRRADELWMTSS
TKEIMPIVMLDGAPVGGGAPGPLAQKFDAAYAAFKREVMRA

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=287, Percent_Identity=25.0871080139373, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787338, Length=256, Percent_Identity=27.734375, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 30694; Mature: 30563

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVYLNGRFQPLAEATIPVLDRGFVFGDGVYELVPVYSRKPFRLDAHLTRLQHSLDGIRL
CEEEECCCCCCHHHHHCCHHCCCEEECCCHHEEHHHCCCCCEEEHHHHHHHHHHHCCEEE
SNPHTPTQWRDLIQHLIAEQDFDDQSVYIQVTRGTAPRDHAFPVGVPPTVFMFAQALVTA
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
TPAQKAVGVCAVSAVDNRWLRCDIKAISLLPNILLRQQATDAGCAETVMFRDGFLTEGAA
CCHHHHHHEEEEEHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCC
SNIFVVKDGVLLTPPSSNLMLTGVTHDVVLELAATLGVPVEIRAIAEAEVRRADELWMTS
CCEEEEECCEEEECCCCCEEEEECHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCC
STKEIMPIVMLDGAPVGGGAPGPLAQKFDAAYAAFKREVMRA
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVYLNGRFQPLAEATIPVLDRGFVFGDGVYELVPVYSRKPFRLDAHLTRLQHSLDGIRL
EEEECCCCCCHHHHHCCHHCCCEEECCCHHEEHHHCCCCCEEEHHHHHHHHHHHCCEEE
SNPHTPTQWRDLIQHLIAEQDFDDQSVYIQVTRGTAPRDHAFPVGVPPTVFMFAQALVTA
CCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHC
TPAQKAVGVCAVSAVDNRWLRCDIKAISLLPNILLRQQATDAGCAETVMFRDGFLTEGAA
CCHHHHHHEEEEEHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCCC
SNIFVVKDGVLLTPPSSNLMLTGVTHDVVLELAATLGVPVEIRAIAEAEVRRADELWMTS
CCEEEEECCEEEECCCCCEEEEECHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCC
STKEIMPIVMLDGAPVGGGAPGPLAQKFDAAYAAFKREVMRA
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]