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The map label for this gene is mrcA [H]

Identifier: 74316219

GI number: 74316219

Start: 217797

End: 220178

Strand: Reverse

Name: mrcA [H]

Synonym: Tbd_0201

Alternate gene names: 74316219

Gene position: 220178-217797 (Counterclockwise)

Preceding gene: 74316229

Following gene: 74316218

Centisome position: 7.57

GC content: 66.62

Gene sequence:

>2382_bases
ATGTTTTCCAGATGGTGGCATTACGCATTGGCGCTGGTCGTGAGCCTCGGCGTCGTCGGCACTGCGCTCGCCGGCCTCGC
GGCCGCCCTGATCTACCCCAACCTGCCGCCGCTCGAGGCGTTGACCGACTACAAGCCCAAGCTGCCACTGCGCGTCTACA
CCGCGGACGGCGCGCTGATCAGCGAATTCGGCGAGGAGCGGCGCGCGTTCATCGCGATCGACAAGGTTCCGCGCTACATG
AAACAGGCGATCATCGCGGCCGAAGACGAGCGCTTCTACAGCCACGGTGGTGTCGACACCCTTGGCGTGCTGCGCGCTGC
GGGCGCGAATTTGGTGTCGGGCGGGGCAAAGGAAGGCGCGTCGACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGT
CGAACGAGAAGACCCTGAGCCGGAAACTGTCCGAGGCGATGCTCGCGATGAAGATCGAGCACAGCCTTTCAAAGGACAAG
ATACTCGAGCTCTACATCAACCAGATCTACCTCGGGCAGCGTGCCTACGGCTTCGAAGCCGCAGCCCGCGCCTATTTCGG
CAAGCCGATGAAGGAACTCACGCTCGCCGAGTTCGCCATGCTCGCCGGCCTGCCGAAGGCGCCATCACGCTACAACCCGG
TCGTCAATTTTCCGCGCGCGAAGGCACGACAGGAGTATGTGCTCGGGCGCATGCTCAAGCTCGGGTTCATCGACCGGCCG
ACCTGGCAGGCGGCGGTCGGACAAAAGCTCGTCATCCGTGAGGCACGCCAGCACGCGGACGTTGTGGCAGACTACGCCGC
CGAAATGGTGCGCCAGATCCTGTTCGAAAAATACGGGGAAGCGATCTACAGCTCCGGCTACAAGGTGGTGACCACGTTGC
GCCGCAGCCAGCAGAACAGCGGGAACCTCGCCGTCTGGCGCGGCATCGCCGACTATGACCAGCGCCACGGCTACCGCGGG
CCCGAAAAGCAGATTCGCCTGCCTGCCGACAGGGCGGAACGCGACAGCGCGATCGCCACGGCCCTCGCCGACCTCGCGCC
GGTCGGCGGCATGCTGCCGGCCGTCGTCACGCGTGCCGACAAGAAATCCATTCACGCGCAGCTGGCGGACGGCACGGTTG
CCGAGATCTCCGGCAAGTCGCTCAAGTGGGTGGACAACTGGGCCACCGCCAAGAAAGGGCGAAAGCTCGAGCCCGGCGCG
ATCGTCCGCGTCCAGACCGGCGCCGGCAAGGAGCGCTGGCGCCTCGCCCAGCTGCCCGAGGTCGAGGCGGCCCTGGTCGC
GCTCGACCCCAACAGCGGCGCGATCACCGCACTGGTGGGCGGCTTCGACTTCAACCGAAACAAGTTCAACCGCGTGACGC
AGGCGTGGCGGCAACCTGGGTCGAGCTTCAAGCCCTTCATCTATTCGGCGGCCCTGGAAAAGGGCTACACCCCGGCGACC
ATCCTCGACGACGCACCATTGATACTCGCCGCCGAAGAGGCGGGCGGCACGGCGTGGGAGCCACGCAACTACGACGGCAG
CAGCAGCGGGCCCGTGCGCATGCGCACCGCGCTCACCAAATCGCTCAATCTGGTGTCGATCCGCATTCTGCAAGGCATCG
GCCCGCACTATGCACGCGACTACATCCGCCGCTTCGGCTTCGACCCGGCGCGTCACCCGCCCTATCTGACGATGGCGCTC
GGCGCCGGCAGCGTCACGCCGCTGCAACTCGCCTCGGCCTACGGCGTCTTCGCCAACGGCGGTTTCGGGGTCAAGCCCTA
CCTCATCGACAAGGTCTTCGACAAGGACGGCCGCCTGCTGATGCAGGCCAGCGCGCAAAAGGCCGGCGTCAACGCGCCCC
GCGTCATCGACCCGCGCAACGCCTGGCTGATGACGAACATGATGCAGGACGTCGTGCGTTACGGCACCGCGGCGCGCGCC
TCGCAGCTCAAGCGCAGCGACATCGCCGGCAAGACGGGCACGACCAACGATGCGCGCGACACCTGGTTCGCCGGCTACAC
CCCGGACCTCGTCGCGGTCGCTTGGATGGGTTACGACCAGCCCCGCTCGATGGGCCGGCGGGAGACCGGCGCGCAGTCGG
CCTTGCCGGTCTGGATGAGCTTCATGGGGACGGCGCTCAAGGGCATTCCGCAAAAAGGCTGGGCGATGCCCGCCGGCCTC
GTCAGCGTGGAAATCGATCCGGTGACGGGCGCGCGCGCGTCCGGATCGGCGATCGACGAATTTTTCGGCGATCTGTTCGG
CACCGAAGCGGAGTCCATCGTCGAACATTTCTATCAGGAGTTTCCGCCGCCCGACCGCGCCGCGCCGCCGACCGACCTGC
TCGAAGCGCCCACGGAGACGCCCTCCGACCCGGACGCACCACCTCCCGGGATACCGCAGTGA

Upstream 100 bases:

>100_bases
TGCGGCCCGCAGGTCGCGCCGGTCGCATTGCGCGCCCGGCGGACGGGACAGAAGCCCGAAGGTCGCGGCTTGCCCGCCCG
CACATATATAAAGGAACACG

Downstream 100 bases:

>100_bases
AGCACCAGGACATGCGCCGGCTCATCGCCCACGCCGCGGCGCGCATGATCGCCGAGGACGGCAGCCTCGACTACGGCACC
GCCAAGCGCAAGGCAGCCCG

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MFSRWWHYALALVVSLGVVGTALAGLAAALIYPNLPPLEALTDYKPKLPLRVYTADGALISEFGEERRAFIAIDKVPRYM
KQAIIAAEDERFYSHGGVDTLGVLRAAGANLVSGGAKEGASTITMQVARNFFLSNEKTLSRKLSEAMLAMKIEHSLSKDK
ILELYINQIYLGQRAYGFEAAARAYFGKPMKELTLAEFAMLAGLPKAPSRYNPVVNFPRAKARQEYVLGRMLKLGFIDRP
TWQAAVGQKLVIREARQHADVVADYAAEMVRQILFEKYGEAIYSSGYKVVTTLRRSQQNSGNLAVWRGIADYDQRHGYRG
PEKQIRLPADRAERDSAIATALADLAPVGGMLPAVVTRADKKSIHAQLADGTVAEISGKSLKWVDNWATAKKGRKLEPGA
IVRVQTGAGKERWRLAQLPEVEAALVALDPNSGAITALVGGFDFNRNKFNRVTQAWRQPGSSFKPFIYSAALEKGYTPAT
ILDDAPLILAAEEAGGTAWEPRNYDGSSSGPVRMRTALTKSLNLVSIRILQGIGPHYARDYIRRFGFDPARHPPYLTMAL
GAGSVTPLQLASAYGVFANGGFGVKPYLIDKVFDKDGRLLMQASAQKAGVNAPRVIDPRNAWLMTNMMQDVVRYGTAARA
SQLKRSDIAGKTGTTNDARDTWFAGYTPDLVAVAWMGYDQPRSMGRRETGAQSALPVWMSFMGTALKGIPQKGWAMPAGL
VSVEIDPVTGARASGSAIDEFFGDLFGTEAESIVEHFYQEFPPPDRAAPPTDLLEAPTETPSDPDAPPPGIPQ

Sequences:

>Translated_793_residues
MFSRWWHYALALVVSLGVVGTALAGLAAALIYPNLPPLEALTDYKPKLPLRVYTADGALISEFGEERRAFIAIDKVPRYM
KQAIIAAEDERFYSHGGVDTLGVLRAAGANLVSGGAKEGASTITMQVARNFFLSNEKTLSRKLSEAMLAMKIEHSLSKDK
ILELYINQIYLGQRAYGFEAAARAYFGKPMKELTLAEFAMLAGLPKAPSRYNPVVNFPRAKARQEYVLGRMLKLGFIDRP
TWQAAVGQKLVIREARQHADVVADYAAEMVRQILFEKYGEAIYSSGYKVVTTLRRSQQNSGNLAVWRGIADYDQRHGYRG
PEKQIRLPADRAERDSAIATALADLAPVGGMLPAVVTRADKKSIHAQLADGTVAEISGKSLKWVDNWATAKKGRKLEPGA
IVRVQTGAGKERWRLAQLPEVEAALVALDPNSGAITALVGGFDFNRNKFNRVTQAWRQPGSSFKPFIYSAALEKGYTPAT
ILDDAPLILAAEEAGGTAWEPRNYDGSSSGPVRMRTALTKSLNLVSIRILQGIGPHYARDYIRRFGFDPARHPPYLTMAL
GAGSVTPLQLASAYGVFANGGFGVKPYLIDKVFDKDGRLLMQASAQKAGVNAPRVIDPRNAWLMTNMMQDVVRYGTAARA
SQLKRSDIAGKTGTTNDARDTWFAGYTPDLVAVAWMGYDQPRSMGRRETGAQSALPVWMSFMGTALKGIPQKGWAMPAGL
VSVEIDPVTGARASGSAIDEFFGDLFGTEAESIVEHFYQEFPPPDRAAPPTDLLEAPTETPSDPDAPPPGIPQ
>Mature_793_residues
MFSRWWHYALALVVSLGVVGTALAGLAAALIYPNLPPLEALTDYKPKLPLRVYTADGALISEFGEERRAFIAIDKVPRYM
KQAIIAAEDERFYSHGGVDTLGVLRAAGANLVSGGAKEGASTITMQVARNFFLSNEKTLSRKLSEAMLAMKIEHSLSKDK
ILELYINQIYLGQRAYGFEAAARAYFGKPMKELTLAEFAMLAGLPKAPSRYNPVVNFPRAKARQEYVLGRMLKLGFIDRP
TWQAAVGQKLVIREARQHADVVADYAAEMVRQILFEKYGEAIYSSGYKVVTTLRRSQQNSGNLAVWRGIADYDQRHGYRG
PEKQIRLPADRAERDSAIATALADLAPVGGMLPAVVTRADKKSIHAQLADGTVAEISGKSLKWVDNWATAKKGRKLEPGA
IVRVQTGAGKERWRLAQLPEVEAALVALDPNSGAITALVGGFDFNRNKFNRVTQAWRQPGSSFKPFIYSAALEKGYTPAT
ILDDAPLILAAEEAGGTAWEPRNYDGSSSGPVRMRTALTKSLNLVSIRILQGIGPHYARDYIRRFGFDPARHPPYLTMAL
GAGSVTPLQLASAYGVFANGGFGVKPYLIDKVFDKDGRLLMQASAQKAGVNAPRVIDPRNAWLMTNMMQDVVRYGTAARA
SQLKRSDIAGKTGTTNDARDTWFAGYTPDLVAVAWMGYDQPRSMGRRETGAQSALPVWMSFMGTALKGIPQKGWAMPAGL
VSVEIDPVTGARASGSAIDEFFGDLFGTEAESIVEHFYQEFPPPDRAAPPTDLLEAPTETPSDPDAPPPGIPQ

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=829, Percent_Identity=39.4451145958987, Blast_Score=553, Evalue=1e-158,
Organism=Escherichia coli, GI1786343, Length=259, Percent_Identity=35.1351351351351, Blast_Score=133, Evalue=4e-32,
Organism=Escherichia coli, GI1788867, Length=196, Percent_Identity=40.8163265306122, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1789601, Length=166, Percent_Identity=36.7469879518072, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 86437; Mature: 86437

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRWWHYALALVVSLGVVGTALAGLAAALIYPNLPPLEALTDYKPKLPLRVYTADGALI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCHHH
SEFGEERRAFIAIDKVPRYMKQAIIAAEDERFYSHGGVDTLGVLRAAGANLVSGGAKEGA
HHHHHHHHEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCH
STITMQVARNFFLSNEKTLSRKLSEAMLAMKIEHSLSKDKILELYINQIYLGQRAYGFEA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
AARAYFGKPMKELTLAEFAMLAGLPKAPSRYNPVVNFPRAKARQEYVLGRMLKLGFIDRP
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TWQAAVGQKLVIREARQHADVVADYAAEMVRQILFEKYGEAIYSSGYKVVTTLRRSQQNS
CHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GNLAVWRGIADYDQRHGYRGPEKQIRLPADRAERDSAIATALADLAPVGGMLPAVVTRAD
CCEEEECCCHHHHHHCCCCCCHHCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KKSIHAQLADGTVAEISGKSLKWVDNWATAKKGRKLEPGAIVRVQTGAGKERWRLAQLPE
HHHHHHHHCCCCCEECCCCCEEEHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCC
VEAALVALDPNSGAITALVGGFDFNRNKFNRVTQAWRQPGSSFKPFIYSAALEKGYTPAT
CCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCE
ILDDAPLILAAEEAGGTAWEPRNYDGSSSGPVRMRTALTKSLNLVSIRILQGIGPHYARD
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHEEHHHHCCCHHHHHH
YIRRFGFDPARHPPYLTMALGAGSVTPLQLASAYGVFANGGFGVKPYLIDKVFDKDGRLL
HHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEE
MQASAQKAGVNAPRVIDPRNAWLMTNMMQDVVRYGTAARASQLKRSDIAGKTGTTNDARD
EEECHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TWFAGYTPDLVAVAWMGYDQPRSMGRRETGAQSALPVWMSFMGTALKGIPQKGWAMPAGL
CEECCCCCHHHHHHHCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VSVEIDPVTGARASGSAIDEFFGDLFGTEAESIVEHFYQEFPPPDRAAPPTDLLEAPTET
EEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
PSDPDAPPPGIPQ
CCCCCCCCCCCCC
>Mature Secondary Structure
MFSRWWHYALALVVSLGVVGTALAGLAAALIYPNLPPLEALTDYKPKLPLRVYTADGALI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEECCCHHH
SEFGEERRAFIAIDKVPRYMKQAIIAAEDERFYSHGGVDTLGVLRAAGANLVSGGAKEGA
HHHHHHHHEEEEHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCH
STITMQVARNFFLSNEKTLSRKLSEAMLAMKIEHSLSKDKILELYINQIYLGQRAYGFEA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
AARAYFGKPMKELTLAEFAMLAGLPKAPSRYNPVVNFPRAKARQEYVLGRMLKLGFIDRP
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
TWQAAVGQKLVIREARQHADVVADYAAEMVRQILFEKYGEAIYSSGYKVVTTLRRSQQNS
CHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GNLAVWRGIADYDQRHGYRGPEKQIRLPADRAERDSAIATALADLAPVGGMLPAVVTRAD
CCEEEECCCHHHHHHCCCCCCHHCEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KKSIHAQLADGTVAEISGKSLKWVDNWATAKKGRKLEPGAIVRVQTGAGKERWRLAQLPE
HHHHHHHHCCCCCEECCCCCEEEHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCC
VEAALVALDPNSGAITALVGGFDFNRNKFNRVTQAWRQPGSSFKPFIYSAALEKGYTPAT
CCEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCE
ILDDAPLILAAEEAGGTAWEPRNYDGSSSGPVRMRTALTKSLNLVSIRILQGIGPHYARD
EECCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHEEHHHHCCCHHHHHH
YIRRFGFDPARHPPYLTMALGAGSVTPLQLASAYGVFANGGFGVKPYLIDKVFDKDGRLL
HHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCEEECCCCCCCHHHHHHHHCCCCCEE
MQASAQKAGVNAPRVIDPRNAWLMTNMMQDVVRYGTAARASQLKRSDIAGKTGTTNDARD
EEECHHHCCCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TWFAGYTPDLVAVAWMGYDQPRSMGRRETGAQSALPVWMSFMGTALKGIPQKGWAMPAGL
CEECCCCCHHHHHHHCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
VSVEIDPVTGARASGSAIDEFFGDLFGTEAESIVEHFYQEFPPPDRAAPPTDLLEAPTET
EEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCC
PSDPDAPPPGIPQ
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA