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The map label for this gene is nirF [H]

Identifier: 74316093

GI number: 74316093

Start: 76898

End: 78073

Strand: Reverse

Name: nirF [H]

Synonym: Tbd_0075

Alternate gene names: 74316093

Gene position: 78073-76898 (Counterclockwise)

Preceding gene: 74316094

Following gene: 74316092

Centisome position: 2.68

GC content: 67.77

Gene sequence:

>1176_bases
ATGCGCGCTAGGATTCCCGTACTTTTTCTGCTTGCCCTCGGCGCCCTGACGGGCTGCGCCGGCACGAACCTGCGCGGCAC
CGGCGACCTCGGCGTCGTCATCGAACGCGCCAGCGGCAGCGTGCAGATTCTCGACACCAGCCGACGCACCAGCCTGGCGA
AAGTGGAGGGCCTCGGCGACCTCTCCCACGCCTCCGTCAGCTACTCCCGCGACGCGCGCTATGCCTATGTATTCGGCCGC
GACGGCGGATTGAGCAAGATCGATCTGCTAACCCAGCGCATCGATCGCCGCATCATCCAGGGCGGCAACAGCATCGGCGG
CGCCATCTCGGCCGACGGCAAGCTGGTCGCCGTGGCCAACTACCAACCCGGCGGCGTGAAAGTATTCGACGCCGACACGC
TTGCGCTCATCGCCGACATTCCCGCCATCGGCGCCGATGGCGCGCGCTCCAAGGTCGTCGGCCTGGTCGACGCCCCCGGG
CAGCGCTTCGTGTTCAGCCTGTTCGAGGCCGGCGAGATCTGGGTCGCCGACATGCACGAGCCGAAGCGGCCGCTGCTGCG
CAAATTCGCCGCTGCCGGCAAGGAACCTTACGACGGCATGATCACCTCCGACGGCCGCCACTACGCCGCCGGCCTCTTCG
GCGAGGACGGGGTGGCCCTGCTCGATCTGTGGAACCTCGACACCGGCGTCAAGCGCGCGTTGGTCGAGTTCGGCCGCCAC
GGCGAGAAACTGCCGGTCTACAAGATGCCCCACCTGGAAGGCTGGGCCGCGACCGGCGGCCGGATCGTGCTGCCCGCGGT
CGGCAAGCACGAGGTCATCATCGCCGACCAGACCAGCTGGAAGGAAATCGCGCGCGTTCCGGTCGCCGGCCAGGCGGTGT
TCGCGGTGGCACGCCCCGACGGGCGCCAGGTGTGGATCAACTTCGCCTTTCCCGACAACGACACGGTGCAGGTGCTCGAC
CTGCCCTCGCAGCGCATCGTGCAGACCCTGAAGCCCGGCAAGGGGGTGCTGCACCTGGAGTTTTCCCCGCGCGGCGAGCA
GGTCTGGCTTTCGCTGCGCGACGAGAATCGCATCGAGATCTACGACCCCGATACCTTCGCCCGCATCGGCACCCTGCCTG
CGCAGAGCCCGAGCGGAATCTTCTTCACCGTGCGTGCCCACAGGATCGGACTATGA

Upstream 100 bases:

>100_bases
CTTCACGGGCGGCCCGGCACGGCCATGCCGCCATGGAAAAACCTCCTCTCCGAGTCCGAAGCGGACTGGATCGTGGACAA
GCTGCAAAAGGGGTTCCCCG

Downstream 100 bases:

>100_bases
GCGACATCCAACTCAGCCTGCTCAACGATTTCCAGCGCGGCTTTCCGCTCACCTCCACGCCCTTCGATGCCATCGCGCAG
CGCCTCGGGCTGGACGTGGA

Product: heme d1 biosynthesis protein NirF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRARIPVLFLLALGALTGCAGTNLRGTGDLGVVIERASGSVQILDTSRRTSLAKVEGLGDLSHASVSYSRDARYAYVFGR
DGGLSKIDLLTQRIDRRIIQGGNSIGGAISADGKLVAVANYQPGGVKVFDADTLALIADIPAIGADGARSKVVGLVDAPG
QRFVFSLFEAGEIWVADMHEPKRPLLRKFAAAGKEPYDGMITSDGRHYAAGLFGEDGVALLDLWNLDTGVKRALVEFGRH
GEKLPVYKMPHLEGWAATGGRIVLPAVGKHEVIIADQTSWKEIARVPVAGQAVFAVARPDGRQVWINFAFPDNDTVQVLD
LPSQRIVQTLKPGKGVLHLEFSPRGEQVWLSLRDENRIEIYDPDTFARIGTLPAQSPSGIFFTVRAHRIGL

Sequences:

>Translated_391_residues
MRARIPVLFLLALGALTGCAGTNLRGTGDLGVVIERASGSVQILDTSRRTSLAKVEGLGDLSHASVSYSRDARYAYVFGR
DGGLSKIDLLTQRIDRRIIQGGNSIGGAISADGKLVAVANYQPGGVKVFDADTLALIADIPAIGADGARSKVVGLVDAPG
QRFVFSLFEAGEIWVADMHEPKRPLLRKFAAAGKEPYDGMITSDGRHYAAGLFGEDGVALLDLWNLDTGVKRALVEFGRH
GEKLPVYKMPHLEGWAATGGRIVLPAVGKHEVIIADQTSWKEIARVPVAGQAVFAVARPDGRQVWINFAFPDNDTVQVLD
LPSQRIVQTLKPGKGVLHLEFSPRGEQVWLSLRDENRIEIYDPDTFARIGTLPAQSPSGIFFTVRAHRIGL
>Mature_391_residues
MRARIPVLFLLALGALTGCAGTNLRGTGDLGVVIERASGSVQILDTSRRTSLAKVEGLGDLSHASVSYSRDARYAYVFGR
DGGLSKIDLLTQRIDRRIIQGGNSIGGAISADGKLVAVANYQPGGVKVFDADTLALIADIPAIGADGARSKVVGLVDAPG
QRFVFSLFEAGEIWVADMHEPKRPLLRKFAAAGKEPYDGMITSDGRHYAAGLFGEDGVALLDLWNLDTGVKRALVEFGRH
GEKLPVYKMPHLEGWAATGGRIVLPAVGKHEVIIADQTSWKEIARVPVAGQAVFAVARPDGRQVWINFAFPDNDTVQVLD
LPSQRIVQTLKPGKGVLHLEFSPRGEQVWLSLRDENRIEIYDPDTFARIGTLPAQSPSGIFFTVRAHRIGL

Specific function: Required for the biosynthesis of heme d1 of nitrite reductase. Could have a dehydrogenase activity yielding sirohydrochlorin from precorrin-2 or dehydrogenation of propionate side chain C17 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011048
- InterPro:   IPR003143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42137; Mature: 42137

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARIPVLFLLALGALTGCAGTNLRGTGDLGVVIERASGSVQILDTSRRTSLAKVEGLGD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHCCCCC
LSHASVSYSRDARYAYVFGRDGGLSKIDLLTQRIDRRIIQGGNSIGGAISADGKLVAVAN
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEE
YQPGGVKVFDADTLALIADIPAIGADGARSKVVGLVDAPGQRFVFSLFEAGEIWVADMHE
CCCCCEEEECCCCEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEECCCC
PKRPLLRKFAAAGKEPYDGMITSDGRHYAAGLFGEDGVALLDLWNLDTGVKRALVEFGRH
CHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHCC
GEKLPVYKMPHLEGWAATGGRIVLPAVGKHEVIIADQTSWKEIARVPVAGQAVFAVARPD
CCCCCEEECCCCCCEECCCCEEEEECCCCEEEEEECCCCHHHHHCCCCCCCEEEEEECCC
GRQVWINFAFPDNDTVQVLDLPSQRIVQTLKPGKGVLHLEFSPRGEQVWLSLRDENRIEI
CCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCEEEE
YDPDTFARIGTLPAQSPSGIFFTVRAHRIGL
ECCCCHHHHCCCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MRARIPVLFLLALGALTGCAGTNLRGTGDLGVVIERASGSVQILDTSRRTSLAKVEGLGD
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCHHHCCCCC
LSHASVSYSRDARYAYVFGRDGGLSKIDLLTQRIDRRIIQGGNSIGGAISADGKLVAVAN
CCCCEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEE
YQPGGVKVFDADTLALIADIPAIGADGARSKVVGLVDAPGQRFVFSLFEAGEIWVADMHE
CCCCCEEEECCCCEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCEEEECCCC
PKRPLLRKFAAAGKEPYDGMITSDGRHYAAGLFGEDGVALLDLWNLDTGVKRALVEFGRH
CHHHHHHHHHHCCCCCCCCEEECCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHCC
GEKLPVYKMPHLEGWAATGGRIVLPAVGKHEVIIADQTSWKEIARVPVAGQAVFAVARPD
CCCCCEEECCCCCCEECCCCEEEEECCCCEEEEEECCCCHHHHHCCCCCCCEEEEEECCC
GRQVWINFAFPDNDTVQVLDLPSQRIVQTLKPGKGVLHLEFSPRGEQVWLSLRDENRIEI
CCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCEEEE
YDPDTFARIGTLPAQSPSGIFFTVRAHRIGL
ECCCCHHHHCCCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566817; 10984043 [H]