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The map label for this gene is glcF [H]

Identifier: 74316062

GI number: 74316062

Start: 45016

End: 46248

Strand: Reverse

Name: glcF [H]

Synonym: Tbd_0044

Alternate gene names: 74316062

Gene position: 46248-45016 (Counterclockwise)

Preceding gene: 74316063

Following gene: 74316061

Centisome position: 1.59

GC content: 70.4

Gene sequence:

>1233_bases
ATGCAGACCCATCTCGCCGCCCCCTTCCGCGAGACCTCCGAAGGCGAGGTCGCCGAGCGCATCCTGCGCAATTGCGTGCA
CTGCGGCTTCTGTCTTGCGACCTGCCCGACCTACCAGCTCCTCGGCGACGAACTCGACTCACCGCGCGGCCGCATCTATC
TGATGAAACAGGTGCTCGAGGGGGCGACGCCGACGGCCCGGACCCAGCTCCACCTCGATCGTTGCCTGACCTGCCGCAAC
TGCGAGACCACCTGCCCGTCGGGGGTCGAGTACGGCAAGCTGCTCGACATCGGCCGCGCGGTCGTCGAACGCGAGGTCGG
CCGCGGCCCCGCGGACACCGCGCTGCGCGCCGCGCTCAAGAACGGCCTCGGCTCGCGCCGGCTGTTCGGCACGGCGCTGC
GGCTCGGGCGAACGCTGCGGCCGCTGTTGCCCGATTTTCTCAAGGCGCGTGTGCCGGCGGCGGACGCCGCCTCGGTCTGG
CCGCGTGCGCGTCACGACCGGCGCATGCTGGTGCTGGAAGGCTGCGTCCAGCCCGGGCTCAAGCCCAACATCAACGCCGC
CGCCGCGCGCGTCCTCGACCGCCTCGGCGTCTCGCTCTTCGCGGCGCCGGAGGCCGGCTGCTGCGGTGCGCTGGCACACC
ATCTCAACGATGCCGAAACCGCGCTCGCACTCGCACGGCGCAACATCGACGCCTGGATCCCGCACCTCGACGCCGGCGTC
GAGGCGATCGTGATGACCGCGTCGGGCTGCGGCGTCATGGTCAAGGACTACGGCTGGTTGCTGCGCGAGGATGCGGCCTA
CGCGGAAAAGGCGGCACGGGTCGCCGCGGCGACCTTCGACATTTCGGAAATCCTCGGCCGCGAACGCGCGGCGCTGCGGG
CGCTGGCCAAGCGTCCGGCATCGACGCGCATCGCCTACCACCCGCCGTGCACGCTGCAGCACGGGCAGAAGCTCGCGAGC
AGCGGGGTCGAGTCGCTGCTCGCCGACGCCGGTTTCGAATTGGGCCCGGTGGCGGAAAAACACCTGTGCTGCGGCTCGGC
GGGGACCTACTCGATCCTGCAGCCGGAGATCGCCGGCGCACTCAAGGCGCGCAAGCTCGCCCATCTGCAAGCCAGCGCAC
CTTCGGTGATCGCAACCGCGAACATCGGCTGCCTGACGCATCTGCAATCCGGTACCGCGACGCCGGTCAGGCACTGGGTG
GAACTTTTGGACGAGGCGCTGGCCGCATCCTGA

Upstream 100 bases:

>100_bases
GAAGGGCCGGTCGAACGAACCGGCGCACAGGGGCCGATCCTCTCGGTGTATGTGCGCGACCCCGACGGCAACCTGATCGA
ACTGTCCAATCCACGCTGAC

Downstream 100 bases:

>100_bases
AGCGCTTCGAGGAGGACTCCAGAATGATTTTCAAGCAACTGTTCGAGCCGGTCTCGAGCACCTACACCTATCTGCTCGGC
TGCGAGGAAACGGGGGCGGC

Product: glycolate oxidase iron-sulfur subunit

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQTHLAAPFRETSEGEVAERILRNCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQVLEGATPTARTQLHLDRCLTCRN
CETTCPSGVEYGKLLDIGRAVVEREVGRGPADTALRAALKNGLGSRRLFGTALRLGRTLRPLLPDFLKARVPAADAASVW
PRARHDRRMLVLEGCVQPGLKPNINAAAARVLDRLGVSLFAAPEAGCCGALAHHLNDAETALALARRNIDAWIPHLDAGV
EAIVMTASGCGVMVKDYGWLLREDAAYAEKAARVAAATFDISEILGRERAALRALAKRPASTRIAYHPPCTLQHGQKLAS
SGVESLLADAGFELGPVAEKHLCCGSAGTYSILQPEIAGALKARKLAHLQASAPSVIATANIGCLTHLQSGTATPVRHWV
ELLDEALAAS

Sequences:

>Translated_410_residues
MQTHLAAPFRETSEGEVAERILRNCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQVLEGATPTARTQLHLDRCLTCRN
CETTCPSGVEYGKLLDIGRAVVEREVGRGPADTALRAALKNGLGSRRLFGTALRLGRTLRPLLPDFLKARVPAADAASVW
PRARHDRRMLVLEGCVQPGLKPNINAAAARVLDRLGVSLFAAPEAGCCGALAHHLNDAETALALARRNIDAWIPHLDAGV
EAIVMTASGCGVMVKDYGWLLREDAAYAEKAARVAAATFDISEILGRERAALRALAKRPASTRIAYHPPCTLQHGQKLAS
SGVESLLADAGFELGPVAEKHLCCGSAGTYSILQPEIAGALKARKLAHLQASAPSVIATANIGCLTHLQSGTATPVRHWV
ELLDEALAAS
>Mature_410_residues
MQTHLAAPFRETSEGEVAERILRNCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQVLEGATPTARTQLHLDRCLTCRN
CETTCPSGVEYGKLLDIGRAVVEREVGRGPADTALRAALKNGLGSRRLFGTALRLGRTLRPLLPDFLKARVPAADAASVW
PRARHDRRMLVLEGCVQPGLKPNINAAAARVLDRLGVSLFAAPEAGCCGALAHHLNDAETALALARRNIDAWIPHLDAGV
EAIVMTASGCGVMVKDYGWLLREDAAYAEKAARVAAATFDISEILGRERAALRALAKRPASTRIAYHPPCTLQHGQKLAS
SGVESLLADAGFELGPVAEKHLCCGSAGTYSILQPEIAGALKARKLAHLQASAPSVIATANIGCLTHLQSGTATPVRHWV
ELLDEALAAS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=408, Percent_Identity=54.656862745098, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786497, Length=234, Percent_Identity=27.3504273504274, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788576, Length=414, Percent_Identity=22.463768115942, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 43760; Mature: 43760

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTHLAAPFRETSEGEVAERILRNCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHC
GATPTARTQLHLDRCLTCRNCETTCPSGVEYGKLLDIGRAVVEREVGRGPADTALRAALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NGLGSRRLFGTALRLGRTLRPLLPDFLKARVPAADAASVWPRARHDRRMLVLEGCVQPGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCC
KPNINAAAARVLDRLGVSLFAAPEAGCCGALAHHLNDAETALALARRNIDAWIPHLDAGV
CCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHCCCHHCCC
EAIVMTASGCGVMVKDYGWLLREDAAYAEKAARVAAATFDISEILGRERAALRALAKRPA
EEEEEECCCCCEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
STRIAYHPPCTLQHGQKLASSGVESLLADAGFELGPVAEKHLCCGSAGTYSILQPEIAGA
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHCHHHHHH
LKARKLAHLQASAPSVIATANIGCLTHLQSGTATPVRHWVELLDEALAAS
HHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTHLAAPFRETSEGEVAERILRNCVHCGFCLATCPTYQLLGDELDSPRGRIYLMKQVLE
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHC
GATPTARTQLHLDRCLTCRNCETTCPSGVEYGKLLDIGRAVVEREVGRGPADTALRAALK
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NGLGSRRLFGTALRLGRTLRPLLPDFLKARVPAADAASVWPRARHDRRMLVLEGCVQPGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCC
KPNINAAAARVLDRLGVSLFAAPEAGCCGALAHHLNDAETALALARRNIDAWIPHLDAGV
CCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHCCCHHCCC
EAIVMTASGCGVMVKDYGWLLREDAAYAEKAARVAAATFDISEILGRERAALRALAKRPA
EEEEEECCCCCEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
STRIAYHPPCTLQHGQKLASSGVESLLADAGFELGPVAEKHLCCGSAGTYSILQPEIAGA
CCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCHHHCHHHHHH
LKARKLAHLQASAPSVIATANIGCLTHLQSGTATPVRHWVELLDEALAAS
HHHHHHHHHHCCCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]