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The map label for this gene is trmE [H]

Identifier: 73543138

GI number: 73543138

Start: 3799454

End: 3800881

Strand: Reverse

Name: trmE [H]

Synonym: Reut_A3456

Alternate gene names: 73543138

Gene position: 3800881-3799454 (Counterclockwise)

Preceding gene: 73543139

Following gene: 73543134

Centisome position: 99.85

GC content: 66.6

Gene sequence:

>1428_bases
ATGACTGCTCCCCTGCCTCCCATTGCCGCCATTGCCACCGCACCCGGACGCGGCGGCATTGGCGTGGTGCGCGTGTCGGG
CCCGGATGTGCGCCCGGTCATGCACGCCATCTGCGGCCAGGCGCTCAAGCCGCGCCATGCCACTTACCTGCCGTTCCTGG
ACGGCCACGGCAAGGTCATCGACCATGGCCTGGCGCTGTACTTCCCGGGGCCGAATTCCTACACCGGCGAAGAGGTGCTG
GAACTGCAGGGGCACGGCGGGCCGGTGGTCATGCAGATGCTGCTGACACGCTGCTTGCAGGCCGGGCACGGGATCGGCCT
GCGCCTGGCCGAACCCGGCGAGTTCACGCGCCGCGCCTTCCTCAACGACAAGCTCGACCTGGCCCAGGCGGAGGCCGTGG
CCGATCTGATCGAGGCCAGCACCGAAGCCGCCGCGCGTTCAGCGGCCCGGTCGATGGAGGGCGAGTTCTCGCATGCTATT
CACACGCTGGTCGAGAAGGTGATCCACCTGCGCATGCTGGTGGAAGCCACGCTGGATTTCCCGGAAGAAGAGATCGACTT
CCTCGAAGCCTCCGACGCGCGCGGCCAGCTTGCAACGATCCGCAACGATCTCGGCAACGTGCTCGCGCAGGCCCGGCAGG
GCGCGCTGCTGCGCGAAGGCCTGTCGGTCGTGCTCGCCGGCCAACCCAACGTGGGCAAGTCGTCGCTGCTGAACGCACTG
GCCGGCGCCGAACTGGCCATCGTCACGCCGATCGCCGGCACCACGCGCGACCGCGTCAAGGAAACGATCCAGATCGAAGG
CATCCCGCTGCATATCATCGACACCGCAGGGCTGCGCGATGAAGCCACGGACGAGGTCGAGCGCATCGGCATCGAACGTA
CGTGGGACGCGATCCGGCGTGCCGACATCGTGCTGCACCTGGTCGATGCCACCGACTACCTGCGCCACGGCCTGTCCGAA
ATCGACGACGCCATCGATGACCGCCTGAGCGGCCAGTTGCCACCGGGCGCGCCGATCGTGCGCGTGGTCAACAAGATCGA
CGTCGCACCGACGGTGGGGGGCATGATGTTCAGTGGAAACCGTCCGCACGTGGTTGCCGCCAACGGGCCCAATCCCACCG
AGATCTGGATCTCCGCACGCACCGGCTCGGGCATCGAACTGCTGCGCAAGGAACTGCTTCGGCTGGTCGGCTGGCAGTCC
GGCAACGAAGGCACCTTCCTTGCGCGCGAACGGCACCTGACGGCGCTGCGGCAGGCGCAATCGCACCTCGACGTGGCTGC
AGAGCAGAGCGAACGGCAGGCGCAGGCGCTGGACCTGTTTGCGGAGGAGTTGCGGCTGGCACAGGAGCATCTGAACAGCA
TCACCGGGGAGTTCACGAGTGATGATCTGTTGGGGACGATTTTTACGCGGTTTTGTATTGGGAAGTAG

Upstream 100 bases:

>100_bases
GTGAGCCACCTCAAAAGGGCTGCTACACCGCTTTTCTAGCGCGATGCGCTGCACAAGGCGCGCCGCGGCCCCGGTCCGAT
CGATCTGAGACAATAGCGCC

Downstream 100 bases:

>100_bases
TTGCGGCCGCCCTCAAGGTATCGACGCAACTTGTGCTGAATGGGTGCGAGACCTTGCTGACGTCGGCAAAATGGTCCAGA
TCGGCTCGTCCGTACAGCGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MTAPLPPIAAIATAPGRGGIGVVRVSGPDVRPVMHAICGQALKPRHATYLPFLDGHGKVIDHGLALYFPGPNSYTGEEVL
ELQGHGGPVVMQMLLTRCLQAGHGIGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSHAI
HTLVEKVIHLRMLVEATLDFPEEEIDFLEASDARGQLATIRNDLGNVLAQARQGALLREGLSVVLAGQPNVGKSSLLNAL
AGAELAIVTPIAGTTRDRVKETIQIEGIPLHIIDTAGLRDEATDEVERIGIERTWDAIRRADIVLHLVDATDYLRHGLSE
IDDAIDDRLSGQLPPGAPIVRVVNKIDVAPTVGGMMFSGNRPHVVAANGPNPTEIWISARTGSGIELLRKELLRLVGWQS
GNEGTFLARERHLTALRQAQSHLDVAAEQSERQAQALDLFAEELRLAQEHLNSITGEFTSDDLLGTIFTRFCIGK

Sequences:

>Translated_475_residues
MTAPLPPIAAIATAPGRGGIGVVRVSGPDVRPVMHAICGQALKPRHATYLPFLDGHGKVIDHGLALYFPGPNSYTGEEVL
ELQGHGGPVVMQMLLTRCLQAGHGIGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSHAI
HTLVEKVIHLRMLVEATLDFPEEEIDFLEASDARGQLATIRNDLGNVLAQARQGALLREGLSVVLAGQPNVGKSSLLNAL
AGAELAIVTPIAGTTRDRVKETIQIEGIPLHIIDTAGLRDEATDEVERIGIERTWDAIRRADIVLHLVDATDYLRHGLSE
IDDAIDDRLSGQLPPGAPIVRVVNKIDVAPTVGGMMFSGNRPHVVAANGPNPTEIWISARTGSGIELLRKELLRLVGWQS
GNEGTFLARERHLTALRQAQSHLDVAAEQSERQAQALDLFAEELRLAQEHLNSITGEFTSDDLLGTIFTRFCIGK
>Mature_474_residues
TAPLPPIAAIATAPGRGGIGVVRVSGPDVRPVMHAICGQALKPRHATYLPFLDGHGKVIDHGLALYFPGPNSYTGEEVLE
LQGHGGPVVMQMLLTRCLQAGHGIGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSHAIH
TLVEKVIHLRMLVEATLDFPEEEIDFLEASDARGQLATIRNDLGNVLAQARQGALLREGLSVVLAGQPNVGKSSLLNALA
GAELAIVTPIAGTTRDRVKETIQIEGIPLHIIDTAGLRDEATDEVERIGIERTWDAIRRADIVLHLVDATDYLRHGLSEI
DDAIDDRLSGQLPPGAPIVRVVNKIDVAPTVGGMMFSGNRPHVVAANGPNPTEIWISARTGSGIELLRKELLRLVGWQSG
NEGTFLARERHLTALRQAQSHLDVAAEQSERQAQALDLFAEELRLAQEHLNSITGEFTSDDLLGTIFTRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=480, Percent_Identity=36.4583333333333, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI193082988, Length=510, Percent_Identity=34.1176470588235, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI193082993, Length=478, Percent_Identity=35.9832635983264, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI2367268, Length=476, Percent_Identity=55.8823529411765, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI87082120, Length=129, Percent_Identity=37.2093023255814, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=477, Percent_Identity=29.1404612159329, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=30.3212851405622, Blast_Score=205, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45550813, Length=487, Percent_Identity=31.4168377823409, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51145; Mature: 51014

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLPPIAAIATAPGRGGIGVVRVSGPDVRPVMHAICGQALKPRHATYLPFLDGHGKVI
CCCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEECCEECCCCCEE
DHGLALYFPGPNSYTGEEVLELQGHGGPVVMQMLLTRCLQAGHGIGLRLAEPGEFTRRAF
ECCEEEEECCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSHAIHTLVEKVIHLRMLVEATLDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEEIDFLEASDARGQLATIRNDLGNVLAQARQGALLREGLSVVLAGQPNVGKSSLLNAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHH
AGAELAIVTPIAGTTRDRVKETIQIEGIPLHIIDTAGLRDEATDEVERIGIERTWDAIRR
CCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHH
ADIVLHLVDATDYLRHGLSEIDDAIDDRLSGQLPPGAPIVRVVNKIDVAPTVGGMMFSGN
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCC
RPHVVAANGPNPTEIWISARTGSGIELLRKELLRLVGWQSGNEGTFLARERHLTALRQAQ
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
SHLDVAAEQSERQAQALDLFAEELRLAQEHLNSITGEFTSDDLLGTIFTRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPLPPIAAIATAPGRGGIGVVRVSGPDVRPVMHAICGQALKPRHATYLPFLDGHGKVI
CCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEECCEECCCCCEE
DHGLALYFPGPNSYTGEEVLELQGHGGPVVMQMLLTRCLQAGHGIGLRLAEPGEFTRRAF
ECCEEEEECCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHH
LNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSHAIHTLVEKVIHLRMLVEATLDF
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PEEEIDFLEASDARGQLATIRNDLGNVLAQARQGALLREGLSVVLAGQPNVGKSSLLNAL
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHH
AGAELAIVTPIAGTTRDRVKETIQIEGIPLHIIDTAGLRDEATDEVERIGIERTWDAIRR
CCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHH
ADIVLHLVDATDYLRHGLSEIDDAIDDRLSGQLPPGAPIVRVVNKIDVAPTVGGMMFSGN
HHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEECCC
RPHVVAANGPNPTEIWISARTGSGIELLRKELLRLVGWQSGNEGTFLARERHLTALRQAQ
CCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
SHLDVAAEQSERQAQALDLFAEELRLAQEHLNSITGEFTSDDLLGTIFTRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA