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The map label for this gene is acrA [H]

Identifier: 73543122

GI number: 73543122

Start: 3780628

End: 3781875

Strand: Direct

Name: acrA [H]

Synonym: Reut_A3440

Alternate gene names: 73543122

Gene position: 3780628-3781875 (Clockwise)

Preceding gene: 73543120

Following gene: 73543123

Centisome position: 99.32

GC content: 68.59

Gene sequence:

>1248_bases
ATGAGGAAGTCCCAACGTATCCATTGCGTTGCCGTCGCCGCGTTGTCGGCGCTGGCGCTGACCGCTTGCGGCGAGAAAAA
GCCTGAAGGCGGCGCCATGCCCCCGCCGGAAGTGGGCGTTGTCACCGTGACGCCGTCGGCCGTCGCCATCGTCAATGAAT
TGCCGGGCCGCCTGGAAGGCGTGCGTACCGCCGAAGTCCGTGCGCGTGTCGAAGGCATCGTGCTGTCGCGCAACTACACC
GAAGGTGGCGAAGTGAAGGCCGGTCAGGTCATGTTCCGCATCGACCCCGCGCCGTACCAGGCGCAGCTTGCGTCGGCCAA
GGCCGCACTGGAGCGTGCCGAGGCCAACGCCGTGTCGGCACGCCAGAAGGCCGAGCGCTACAAGCCGCTGGTGGCCGTCA
ATGCCGTCAGCAAGCAGGAATATGACGAGGCCGTGGCCGCCGCCGGCCAGGCCAATGCGGACATCGCCTCGGCCAAGGCT
GCCGTGACCACCGCGCAGATCAACCTGGGCTACACCACCGTGACCGCCCCGATCAGCGGCCGCGCCGGACGTGCGCTGGT
GACCGAAGGTGCGCTGGTCGGCAAGGGCGAGGCCACCAAGCTCGCGCTCGTCGAGCAGGTCGACCCGATCTGGGTGACGT
TCACCCAGCCCGCCGCTGAAGTCACGCGCCTGCGCCGCGCCATCGAGTCCGGCGCGGTCAAGGGCGTGGACGGCGGCGCC
AGGGTGCACCTGTATGTCGAAGATGGCCACGAATACGAACAGACCGGCAAGCTGCTGTTCTCGGACATGACCGTGGACCC
GACCACCGGCGCCATCACGCTGCGCGCGCAGTTCCCGAACCCGAAGCGCGAGCTGCTGTCGGGGACTTTCGTGCGCGTGA
AGATCGAGCAGGGCGTGGATGAGAACGCGATGACCGTGCCGCAGCGCGCGCTGATCCGCAATGCCCAGGGCGCCAGCGTG
CTGGTGGTGGGCAATGACGGCAACGTGGCCGCTCTGCCGGTGAAGGCGCCAAAGGCGATCGGCGACCGCTGGATCGTGAC
CGAAGGCCTCAAGGGCGGCGAGAAGGTGATCGTCGAGGGCCTGCAGAAGGTCAAGGTCGGCGCGCCGGCCAAGGCCGTGC
CGTTCCAGCCGGCCGGTGCTCCGGCGCCTGCCAAGCAGGCTTCGGAGCCGCAACCGGCTTCGGGCAGCAAGGCCGGTGCC
AAGGCGGAAGCCCAGGCTGACGCCAAGTCCGACGCCAAGCAAGGCTAA

Upstream 100 bases:

>100_bases
GGCACGCAGCGGACCGCATCGCGCCCCGATTGCCCCCTATTTCCACCAGGCATGCCGGGACCGGCGCGGCAGATCTCAAA
ATTTCATCATGGGGTTATCG

Downstream 100 bases:

>100_bases
ACCCAGCGCATCAGACAGAGGGAGCCAGTTTTATGGCCAAGTTTTTCATCGACCGGCCGGTGTTCGCGTGGGTGCTCGCG
CTGATCATCGTGCTGGGCGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MRKSQRIHCVAVAALSALALTACGEKKPEGGAMPPPEVGVVTVTPSAVAIVNELPGRLEGVRTAEVRARVEGIVLSRNYT
EGGEVKAGQVMFRIDPAPYQAQLASAKAALERAEANAVSARQKAERYKPLVAVNAVSKQEYDEAVAAAGQANADIASAKA
AVTTAQINLGYTTVTAPISGRAGRALVTEGALVGKGEATKLALVEQVDPIWVTFTQPAAEVTRLRRAIESGAVKGVDGGA
RVHLYVEDGHEYEQTGKLLFSDMTVDPTTGAITLRAQFPNPKRELLSGTFVRVKIEQGVDENAMTVPQRALIRNAQGASV
LVVGNDGNVAALPVKAPKAIGDRWIVTEGLKGGEKVIVEGLQKVKVGAPAKAVPFQPAGAPAPAKQASEPQPASGSKAGA
KAEAQADAKSDAKQG

Sequences:

>Translated_415_residues
MRKSQRIHCVAVAALSALALTACGEKKPEGGAMPPPEVGVVTVTPSAVAIVNELPGRLEGVRTAEVRARVEGIVLSRNYT
EGGEVKAGQVMFRIDPAPYQAQLASAKAALERAEANAVSARQKAERYKPLVAVNAVSKQEYDEAVAAAGQANADIASAKA
AVTTAQINLGYTTVTAPISGRAGRALVTEGALVGKGEATKLALVEQVDPIWVTFTQPAAEVTRLRRAIESGAVKGVDGGA
RVHLYVEDGHEYEQTGKLLFSDMTVDPTTGAITLRAQFPNPKRELLSGTFVRVKIEQGVDENAMTVPQRALIRNAQGASV
LVVGNDGNVAALPVKAPKAIGDRWIVTEGLKGGEKVIVEGLQKVKVGAPAKAVPFQPAGAPAPAKQASEPQPASGSKAGA
KAEAQADAKSDAKQG
>Mature_415_residues
MRKSQRIHCVAVAALSALALTACGEKKPEGGAMPPPEVGVVTVTPSAVAIVNELPGRLEGVRTAEVRARVEGIVLSRNYT
EGGEVKAGQVMFRIDPAPYQAQLASAKAALERAEANAVSARQKAERYKPLVAVNAVSKQEYDEAVAAAGQANADIASAKA
AVTTAQINLGYTTVTAPISGRAGRALVTEGALVGKGEATKLALVEQVDPIWVTFTQPAAEVTRLRRAIESGAVKGVDGGA
RVHLYVEDGHEYEQTGKLLFSDMTVDPTTGAITLRAQFPNPKRELLSGTFVRVKIEQGVDENAMTVPQRALIRNAQGASV
LVVGNDGNVAALPVKAPKAIGDRWIVTEGLKGGEKVIVEGLQKVKVGAPAKAVPFQPAGAPAPAKQASEPQPASGSKAGA
KAEAQADAKSDAKQG

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=49.8666666666667, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1789665, Length=380, Percent_Identity=47.3684210526316, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=44.5652173913043, Blast_Score=317, Evalue=7e-88,
Organism=Escherichia coli, GI87082036, Length=326, Percent_Identity=28.8343558282209, Blast_Score=154, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43004; Mature: 43004

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSQRIHCVAVAALSALALTACGEKKPEGGAMPPPEVGVVTVTPSAVAIVNELPGRLEG
CCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCHHCC
VRTAEVRARVEGIVLSRNYTEGGEVKAGQVMFRIDPAPYQAQLASAKAALERAEANAVSA
CCHHHHHHEEEEEEEECCCCCCCCEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RQKAERYKPLVAVNAVSKQEYDEAVAAAGQANADIASAKAAVTTAQINLGYTTVTAPISG
HHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEEEECEEEEEECCCC
RAGRALVTEGALVGKGEATKLALVEQVDPIWVTFTQPAAEVTRLRRAIESGAVKGVDGGA
CCCCEEEECCEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCEECCCCCC
RVHLYVEDGHEYEQTGKLLFSDMTVDPTTGAITLRAQFPNPKRELLSGTFVRVKIEQGVD
EEEEEEECCCCHHHHCCEEEECCEECCCCCEEEEEEECCCHHHHHHCCCEEEEEEECCCC
ENAMTVPQRALIRNAQGASVLVVGNDGNVAALPVKAPKAIGDRWIVTEGLKGGEKVIVEG
CCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEECCCCHHCCCEEEEECCCCCCHHHHHHH
LQKVKVGAPAKAVPFQPAGAPAPAKQASEPQPASGSKAGAKAEAQADAKSDAKQG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MRKSQRIHCVAVAALSALALTACGEKKPEGGAMPPPEVGVVTVTPSAVAIVNELPGRLEG
CCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCHHCC
VRTAEVRARVEGIVLSRNYTEGGEVKAGQVMFRIDPAPYQAQLASAKAALERAEANAVSA
CCHHHHHHEEEEEEEECCCCCCCCEEECEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
RQKAERYKPLVAVNAVSKQEYDEAVAAAGQANADIASAKAAVTTAQINLGYTTVTAPISG
HHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCHHHCCEEEEEEEEEECEEEEEECCCC
RAGRALVTEGALVGKGEATKLALVEQVDPIWVTFTQPAAEVTRLRRAIESGAVKGVDGGA
CCCCEEEECCEEECCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCEECCCCCC
RVHLYVEDGHEYEQTGKLLFSDMTVDPTTGAITLRAQFPNPKRELLSGTFVRVKIEQGVD
EEEEEEECCCCHHHHCCEEEECCEECCCCCEEEEEEECCCHHHHHHCCCEEEEEEECCCC
ENAMTVPQRALIRNAQGASVLVVGNDGNVAALPVKAPKAIGDRWIVTEGLKGGEKVIVEG
CCCCCCCHHHHHCCCCCCEEEEEECCCCEEEEECCCCHHCCCEEEEECCCCCCHHHHHHH
LQKVKVGAPAKAVPFQPAGAPAPAKQASEPQPASGSKAGAKAEAQADAKSDAKQG
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]