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The map label for this gene is pckG [H]

Identifier: 73543102

GI number: 73543102

Start: 3752926

End: 3754782

Strand: Reverse

Name: pckG [H]

Synonym: Reut_A3420

Alternate gene names: 73543102

Gene position: 3754782-3752926 (Counterclockwise)

Preceding gene: 73543118

Following gene: 73543099

Centisome position: 98.64

GC content: 65.27

Gene sequence:

>1857_bases
ATGAACCACCCCTCGATGCAAGGCACGACGGCGTTGAACGTCCCGGCCTGGGTCAGGAACCAGAAGCTGGTGGCCTGGGT
TGCGGAGATCGCCGCGCTGACCAAGCCGGAGCGCATTCACTGGTGCGACGGTTCGCAGGAAGAATATGATCGCCTCTGCG
AACAGATGGTCGCCGCGGGCACGCTCAAGCGCCTGAATCCCGCCAAGCGCAAGAACTCTTACCTCGCCCTGTCCGACCCG
TCCGACGTCGCACGTGTCGAAGACCGCACCTTCATCTGCTCGCAGAAGAAGGAAGACGCCGGCCCGACCAACAACTGGGT
GGCTCCGGCCGAAATGCGCACGACGCTCAACGGCCTGTTCGACGGCTGCATGCGCGGCCGCACGCTGTACGTGGTGCCGT
TCTCGATGGGCCCGCTGGGCTCGCCGATCGCCCACATTGGCGTGGAGCTGTCCGATTCCCCGTACGTGGCCGTCAACATG
CGCATCATGACGCGCATGGGCAAGGCCGTGTACGACGTGCTGGGCACCGATGGCGACTTCGTCCCGTGCGTGCACACCGT
AGGCAAGCCGCTCGCCGCCGGCGAGAAGGATGTGCCGTGGCCGTGCAACCCGACCAAGTACATCGTCCACTTCCCGGAGT
CGCGCGAGATCTGGTCGTTCGGCTCGGGGTACGGCGGCAACGCGCTGCTCGGCAAGAAGTGCTTCGCACTGCGTATCGCA
TCGACCATGGGCCGCGACGAAGGCTGGCTGGCCGAGCACATGCTGATCCTGGGCGTGACTTCGCCCGAGGGCAAGAAATT
CCATGTTGCGGCCGCGTTCCCGTCGGCCTGCGGCAAGACCAACTTCGCCATGCTGATCCCGCCCAAGGGATTCGAAGGCT
GGAAGGTCACGACAATCGGCGACGACATCGCGTGGATCAAGCCGGGCAAGGACGGCCGCCTGTACGCGATCAACCCGGAA
GCCGGCTACTTCGGCGTGGCCCCGGGCACGAGCGAGAAGACCAACTTCAACGCCATGGCGACGCTGAAGGAAAACGTCAT
CTTCACCAATGTGGCGCTGACCGACGACGGCGACGTGTGGTGGGAGGGCATGACCAAGGAAGCGCCGGCGCATCTGACCG
ACTGGCAGGGCAAGGACTGGACCCCGGAGATCGCCAAGGCCACCGGCGCCAAGGCCGCGCACCCGAACGCCCGCTTCACC
GCCCCGGCATCGCAGTGCCCGTCGATCGACGAGAATTGGGACAACCCGGCAGGCGTACCCATTGATGCGTTCATCTTCGG
CGGCCGCCGCTCGACCACCGTGCCGCTGGTGACTGAGGCGCGCAATTGGACCGAAGGCGTGTACATGGCTGCGACCATGG
GCTCCGAAACCACCGCTGCGGCCGCTGGCCAGCAGGGCGTGGTGCGCCGCGACCCGTTCGCCATGCTGCCGTTCTGCGGC
TACAACATGAGCGACTATTTTGGCCACTGGCTCGCACTGGGCCAAAAGCTCGAAGCCGCTGGCGCGAAGCTGCCGAAAAT
CTACTGTGTGAACTGGTTCCGCAAGGACGCCGACGGCAACTTCGTGTGGCCGGGCTTTGGCGAGAACATGCGCGTGCTGT
CGTGGATGATCGACCGCGTGGAAGGCAAAGGCGAGGGCGCCGAGCACGTGTTCGGGACGAGCCCGCGCTACGAAGACCTG
AACTGGAGCGGCGTGGAATTCTCCGTGGCGCAGTTCACGCAGGTCACGTCGATCGATGCCGATGCCTGGAAGCAGGAACT
GGCGCTGCACGACGAACTGTTCACGCAACTGAAGCACAACCTGCCACAGGCGCTGGCCGAAGCGCGCGCGGCGCTGGGCA
AGCGGCTGGAGGGCTAA

Upstream 100 bases:

>100_bases
CGACCGGTGTGAGCCACAGGACACGCCGGCCTTGCCGGCCAAGGACCGGGCTTTGAAAAGATTTCCCCTCTTCCTTCCCT
TTACACTTTGGAGTTGCAGT

Downstream 100 bases:

>100_bases
AGTCTCGCTGCCACGTTGCTGTCCTGCGGATTTGAGGACAGCAAGACAAAACGCCCGTCTCATCCGGAGACGGGCGTTTT
TGTTTTCCTATCCGCCCTCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MNHPSMQGTTALNVPAWVRNQKLVAWVAEIAALTKPERIHWCDGSQEEYDRLCEQMVAAGTLKRLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKKEDAGPTNNWVAPAEMRTTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGKAVYDVLGTDGDFVPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKFHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLTDWQGKDWTPEIAKATGAKAAHPNARFT
APASQCPSIDENWDNPAGVPIDAFIFGGRRSTTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGQKLEAAGAKLPKIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGKGEGAEHVFGTSPRYEDL
NWSGVEFSVAQFTQVTSIDADAWKQELALHDELFTQLKHNLPQALAEARAALGKRLEG

Sequences:

>Translated_618_residues
MNHPSMQGTTALNVPAWVRNQKLVAWVAEIAALTKPERIHWCDGSQEEYDRLCEQMVAAGTLKRLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKKEDAGPTNNWVAPAEMRTTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGKAVYDVLGTDGDFVPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKFHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLTDWQGKDWTPEIAKATGAKAAHPNARFT
APASQCPSIDENWDNPAGVPIDAFIFGGRRSTTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGQKLEAAGAKLPKIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGKGEGAEHVFGTSPRYEDL
NWSGVEFSVAQFTQVTSIDADAWKQELALHDELFTQLKHNLPQALAEARAALGKRLEG
>Mature_618_residues
MNHPSMQGTTALNVPAWVRNQKLVAWVAEIAALTKPERIHWCDGSQEEYDRLCEQMVAAGTLKRLNPAKRKNSYLALSDP
SDVARVEDRTFICSQKKEDAGPTNNWVAPAEMRTTLNGLFDGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNM
RIMTRMGKAVYDVLGTDGDFVPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIA
STMGRDEGWLAEHMLILGVTSPEGKKFHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIGDDIAWIKPGKDGRLYAINPE
AGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVWWEGMTKEAPAHLTDWQGKDWTPEIAKATGAKAAHPNARFT
APASQCPSIDENWDNPAGVPIDAFIFGGRRSTTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
YNMSDYFGHWLALGQKLEAAGAKLPKIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRVEGKGEGAEHVFGTSPRYEDL
NWSGVEFSVAQFTQVTSIDADAWKQELALHDELFTQLKHNLPQALAEARAALGKRLEG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=609, Percent_Identity=50.7389162561576, Blast_Score=603, Evalue=1e-172,
Organism=Homo sapiens, GI66346721, Length=607, Percent_Identity=51.5650741350906, Blast_Score=599, Evalue=1e-171,
Organism=Homo sapiens, GI66346723, Length=378, Percent_Identity=54.7619047619048, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71989645, Length=612, Percent_Identity=48.5294117647059, Blast_Score=574, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989658, Length=612, Percent_Identity=48.5294117647059, Blast_Score=573, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI17555492, Length=644, Percent_Identity=48.2919254658385, Blast_Score=571, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI71989653, Length=599, Percent_Identity=48.7479131886477, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564411, Length=585, Percent_Identity=51.1111111111111, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564407, Length=530, Percent_Identity=51.1320754716981, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=486, Percent_Identity=51.8518518518518, Blast_Score=498, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI32564409, Length=375, Percent_Identity=48, Blast_Score=327, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17540956, Length=523, Percent_Identity=25.6214149139579, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24655085, Length=607, Percent_Identity=50.4118616144975, Blast_Score=589, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=605, Percent_Identity=50.2479338842975, Blast_Score=587, Evalue=1e-168,
Organism=Drosophila melanogaster, GI161077226, Length=575, Percent_Identity=50.9565217391304, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24655088, Length=511, Percent_Identity=52.2504892367906, Blast_Score=515, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67726; Mature: 67726

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00505 PEPCK_GTP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHPSMQGTTALNVPAWVRNQKLVAWVAEIAALTKPERIHWCDGSQEEYDRLCEQMVAAG
CCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
TLKRLNPAKRKNSYLALSDPSDVARVEDRTFICSQKKEDAGPTNNWVAPAEMRTTLNGLF
HHHHCCHHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGKAVYDVLGTDGDF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHEEEHH
STMGRDEGWLAEHMLILGVTSPEGKKFHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIG
HHCCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTKEAPAHLTDWQGKDWTPEIAKATGAKAAHPNARFTAPASQCPSIDENWDNPAGVP
ECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
IDAFIFGGRRSTTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLALGQKLEAAGAKLPKIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRV
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
EGKGEGAEHVFGTSPRYEDLNWSGVEFSVAQFTQVTSIDADAWKQELALHDELFTQLKHN
CCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LPQALAEARAALGKRLEG
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNHPSMQGTTALNVPAWVRNQKLVAWVAEIAALTKPERIHWCDGSQEEYDRLCEQMVAAG
CCCCCCCCCEEECCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
TLKRLNPAKRKNSYLALSDPSDVARVEDRTFICSQKKEDAGPTNNWVAPAEMRTTLNGLF
HHHHCCHHHHCCCEEEECCCCHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
DGCMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVAVNMRIMTRMGKAVYDVLGTDGDF
HHHHCCCEEEEEEECCCCCCCCHHHEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCCCE
VPCVHTVGKPLAAGEKDVPWPCNPTKYIVHFPESREIWSFGSGYGGNALLGKKCFALRIA
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCCCCCCCCHHCCHHHEEEHH
STMGRDEGWLAEHMLILGVTSPEGKKFHVAAAFPSACGKTNFAMLIPPKGFEGWKVTTIG
HHCCCCCCHHEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEEEC
DDIAWIKPGKDGRLYAINPEAGYFGVAPGTSEKTNFNAMATLKENVIFTNVALTDDGDVW
CCEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEEECCEEEEEEEEECCCCEE
WEGMTKEAPAHLTDWQGKDWTPEIAKATGAKAAHPNARFTAPASQCPSIDENWDNPAGVP
ECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHCCCCCCCCCCCCCCC
IDAFIFGGRRSTTVPLVTEARNWTEGVYMAATMGSETTAAAAGQQGVVRRDPFAMLPFCG
EEEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHCCCCCCEECCCCEECCCCC
YNMSDYFGHWLALGQKLEAAGAKLPKIYCVNWFRKDADGNFVWPGFGENMRVLSWMIDRV
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
EGKGEGAEHVFGTSPRYEDLNWSGVEFSVAQFTQVTSIDADAWKQELALHDELFTQLKHN
CCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LPQALAEARAALGKRLEG
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA