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The map label for this gene is rsmB [H]

Identifier: 73543087

GI number: 73543087

Start: 3735682

End: 3737055

Strand: Reverse

Name: rsmB [H]

Synonym: Reut_A3405

Alternate gene names: 73543087

Gene position: 3737055-3735682 (Counterclockwise)

Preceding gene: 73543088

Following gene: 73543086

Centisome position: 98.17

GC content: 67.9

Gene sequence:

>1374_bases
ATGCGCCTGCCCCACGAATCGCTTGCCTTCCAGATGCTCGGTGCCGCCATGGCCGTGCGTGCCGTCAATGAAGGCACGGC
CCTGCCGCAGGCCATTGAAGATACCGCCGCGCACCTGCGCCTGGACCGCGTGCGCGATGCCGCCACGCGCGGCGCCGTGC
AGGACCTGGCCTACCGCACCATGCGCCAGTTCGGCACGGCGCGAGCGCTGGTCACGGAACTGGTCACGCGCCCGCCCGGC
GCCCAGGTCGATTCGCTGCTCGCCGTGGCGTTGTCGCTGCTGCTCGAAGGCGAGGAGGGCGATGCGGCCAAGGCCGGCCG
CGGCGGTTATAACGCATTCACGGTGGTGGACCAGGCCGTCAGCGCCGCGGCATCCGAACCCAAGACTGCGCATGCGCGCG
GCCTCGTGAACGCCGTGCTGCGGCGTTTCCTGCGTGAACGCAAGGCGCTGCTCGCCGAAGTCAGCCGCGACGACCAGGCG
CGCTGGAACCTGCCCGAATGGTGGCTGCGCATGCTGCGCGAAGCCTATCCCGACCAGTGGACAGCGCTCGCCGAAAGCGC
GAACGTGCGTCCGCCTATGACACTGCGCGTCAATACGGCACGCATCCCGTTGCAGCAATACCTGACGGATCTTGCCAATG
CCGGACTGGCCGGCCAGGCCATCGGCACGCAGGCCGTGCGACTGGTTCGCGCAGTGCCGGTCTCCCAAGTCCCGGGATTT
GATCGCGGCGATGTCTCGGTACAGGACGCCGGCGCGCAGCTCGCGGCGCCGCTGCTCGAGGTAGCCGACGGCATGCGCGT
GCTCGACGCGTGCGCCGCGCCCGGCGGCAAGACGGGGCATCTGCTGGAGTTGGCCGATATCGACGTGACTGCCGTGGAGA
GCGACGCGCAACGTGCGTCGCGCATCGACGAAAACCTGTCGCGCCTCGGCAAGCGCGCGAGCATCGTCGTGGGCGACGCG
AGCCGCCCCGGCGACTGGTGGGATGGCCAGCAGTTCGACCGCATCCTGGCCGACGTGCCGTGCTCCGCATCGGGCATCGT
GCGGCGCCACCCCGATATTCGCTGGCTGCGCCGTGAAGCCGATATTGCCCGGCTTGTCAATGAACAGCGGCGCATCGTTT
CGCAACTCTGGCCGCTGCTCAAGCCTGGCGGCATTCTGGTCTATGTCACCTGTTCTATTTTCCCAACGGAAGGGGAGGAG
CAGGCGCGCTGGTTTGGTGCACAGTTGGCAGATGCGATACGATTGCAGGCGCCGGGGCAGCTGCTGCCCGGCACGCATGC
AACACAGCCTGCCGGCGACAACCATGCTGGCCAGGTCAGCCTGCCATCTGATCATGATGGCTTCTTCTACGCCCGCTTCC
AGAAACGCGCCTGA

Upstream 100 bases:

>100_bases
GCTTTTTCTTGCCTGCGTGGCGCCGCCTGCGCCTGCATGGCCAGGCGGCCGTTGCCGCGCCTGCTTTGTTCTATTGTCTG
AAGTCTGAAGCCTTACCGAT

Downstream 100 bases:

>100_bases
TTACGCGCCTGATCCGATCCGATGGTGAGCACGCTGCGCCTGTCAGCCTTCCGCGCCCCTGCCGACCAAGCGCACACGGT
TGCCGGGGCGTGGTGCAGTG

Product: Fmu, rRNA SAM-dependent methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRLPHESLAFQMLGAAMAVRAVNEGTALPQAIEDTAAHLRLDRVRDAATRGAVQDLAYRTMRQFGTARALVTELVTRPPG
AQVDSLLAVALSLLLEGEEGDAAKAGRGGYNAFTVVDQAVSAAASEPKTAHARGLVNAVLRRFLRERKALLAEVSRDDQA
RWNLPEWWLRMLREAYPDQWTALAESANVRPPMTLRVNTARIPLQQYLTDLANAGLAGQAIGTQAVRLVRAVPVSQVPGF
DRGDVSVQDAGAQLAAPLLEVADGMRVLDACAAPGGKTGHLLELADIDVTAVESDAQRASRIDENLSRLGKRASIVVGDA
SRPGDWWDGQQFDRILADVPCSASGIVRRHPDIRWLRREADIARLVNEQRRIVSQLWPLLKPGGILVYVTCSIFPTEGEE
QARWFGAQLADAIRLQAPGQLLPGTHATQPAGDNHAGQVSLPSDHDGFFYARFQKRA

Sequences:

>Translated_457_residues
MRLPHESLAFQMLGAAMAVRAVNEGTALPQAIEDTAAHLRLDRVRDAATRGAVQDLAYRTMRQFGTARALVTELVTRPPG
AQVDSLLAVALSLLLEGEEGDAAKAGRGGYNAFTVVDQAVSAAASEPKTAHARGLVNAVLRRFLRERKALLAEVSRDDQA
RWNLPEWWLRMLREAYPDQWTALAESANVRPPMTLRVNTARIPLQQYLTDLANAGLAGQAIGTQAVRLVRAVPVSQVPGF
DRGDVSVQDAGAQLAAPLLEVADGMRVLDACAAPGGKTGHLLELADIDVTAVESDAQRASRIDENLSRLGKRASIVVGDA
SRPGDWWDGQQFDRILADVPCSASGIVRRHPDIRWLRREADIARLVNEQRRIVSQLWPLLKPGGILVYVTCSIFPTEGEE
QARWFGAQLADAIRLQAPGQLLPGTHATQPAGDNHAGQVSLPSDHDGFFYARFQKRA
>Mature_457_residues
MRLPHESLAFQMLGAAMAVRAVNEGTALPQAIEDTAAHLRLDRVRDAATRGAVQDLAYRTMRQFGTARALVTELVTRPPG
AQVDSLLAVALSLLLEGEEGDAAKAGRGGYNAFTVVDQAVSAAASEPKTAHARGLVNAVLRRFLRERKALLAEVSRDDQA
RWNLPEWWLRMLREAYPDQWTALAESANVRPPMTLRVNTARIPLQQYLTDLANAGLAGQAIGTQAVRLVRAVPVSQVPGF
DRGDVSVQDAGAQLAAPLLEVADGMRVLDACAAPGGKTGHLLELADIDVTAVESDAQRASRIDENLSRLGKRASIVVGDA
SRPGDWWDGQQFDRILADVPCSASGIVRRHPDIRWLRREADIARLVNEQRRIVSQLWPLLKPGGILVYVTCSIFPTEGEE
QARWFGAQLADAIRLQAPGQLLPGTHATQPAGDNHAGQVSLPSDHDGFFYARFQKRA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=273, Percent_Identity=28.9377289377289, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI76150623, Length=273, Percent_Identity=28.9377289377289, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI32698918, Length=189, Percent_Identity=32.2751322751323, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI301336155, Length=176, Percent_Identity=34.6590909090909, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI39995082, Length=176, Percent_Identity=34.6590909090909, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367212, Length=447, Percent_Identity=39.1498881431767, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87081985, Length=308, Percent_Identity=29.2207792207792, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=267, Percent_Identity=28.0898876404494, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25143471, Length=200, Percent_Identity=34, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=27.3648648648649, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319447, Length=304, Percent_Identity=28.2894736842105, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24668781, Length=293, Percent_Identity=32.7645051194539, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI22024126, Length=282, Percent_Identity=28.3687943262411, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356513, Length=293, Percent_Identity=32.7645051194539, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355201, Length=202, Percent_Identity=33.1683168316832, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=32.5301204819277, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 49614; Mature: 49614

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPHESLAFQMLGAAMAVRAVNEGTALPQAIEDTAAHLRLDRVRDAATRGAVQDLAYRT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRQFGTARALVTELVTRPPGAQVDSLLAVALSLLLEGEEGDAAKAGRGGYNAFTVVDQAV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
SAAASEPKTAHARGLVNAVLRRFLRERKALLAEVSRDDQARWNLPEWWLRMLREAYPDQW
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHH
TALAESANVRPPMTLRVNTARIPLQQYLTDLANAGLAGQAIGTQAVRLVRAVPVSQVPGF
HHHHHCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCC
DRGDVSVQDAGAQLAAPLLEVADGMRVLDACAAPGGKTGHLLELADIDVTAVESDAQRAS
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECHHHHHHH
RIDENLSRLGKRASIVVGDASRPGDWWDGQQFDRILADVPCSASGIVRRHPDIRWLRREA
HHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHH
DIARLVNEQRRIVSQLWPLLKPGGILVYVTCSIFPTEGEEQARWFGAQLADAIRLQAPGQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LLPGTHATQPAGDNHAGQVSLPSDHDGFFYARFQKRA
CCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MRLPHESLAFQMLGAAMAVRAVNEGTALPQAIEDTAAHLRLDRVRDAATRGAVQDLAYRT
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRQFGTARALVTELVTRPPGAQVDSLLAVALSLLLEGEEGDAAKAGRGGYNAFTVVDQAV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
SAAASEPKTAHARGLVNAVLRRFLRERKALLAEVSRDDQARWNLPEWWLRMLREAYPDQW
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHH
TALAESANVRPPMTLRVNTARIPLQQYLTDLANAGLAGQAIGTQAVRLVRAVPVSQVPGF
HHHHHCCCCCCCEEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHCCCC
DRGDVSVQDAGAQLAAPLLEVADGMRVLDACAAPGGKTGHLLELADIDVTAVESDAQRAS
CCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEECHHHHHHH
RIDENLSRLGKRASIVVGDASRPGDWWDGQQFDRILADVPCSASGIVRRHPDIRWLRREA
HHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHH
DIARLVNEQRRIVSQLWPLLKPGGILVYVTCSIFPTEGEEQARWFGAQLADAIRLQAPGQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCC
LLPGTHATQPAGDNHAGQVSLPSDHDGFFYARFQKRA
CCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]