BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is braG [H]

Identifier: 73543052

GI number: 73543052

Start: 3689752

End: 3690477

Strand: Reverse

Name: braG [H]

Synonym: Reut_A3370

Alternate gene names: 73543052

Gene position: 3690477-3689752 (Counterclockwise)

Preceding gene: 73543053

Following gene: 73543051

Centisome position: 96.95

GC content: 64.74

Gene sequence:

>726_bases
ATGACGACATCCAACGTACCCGCGCTTGAAATCAAGGACCTGCACGCCTGGTACGGCGAATCGCACATCCTGCACGGCGT
GGACCTGACCGTGAACCGCGGCGAGGTGGTCACGCTGCTGGGCCGCAATGGCGCGGGCCGCACCACGACGCTGCGCGCGA
TCATGGGCCTCACTGGCGCGCGCAAGGGCTCGATCAAGGTCAACGGCCACGAGACCATCGGCCTGCCCACGCACAAGATC
GCGCACTACGGCATTGGCTACTGTCCGGAAGAGCGGGCGATCTTCTCGAGCCTGTCATGTGAAGAGAACCTGATGCTGCC
ACCGGTGCTCAAGGGCGCAGACAGCAGCAAGGGCATGAGCGAAGCCGAGATCTACGACATGTTCCCGAACCTGAAGGAGC
GCCGCCAGAGCCAGGGCACGCGTCTGTCGGGCGGCGAACAGCAGATGCTGGCGGTAGGGCGCATCCTGCGCACCGGCGCG
AACCTGCTGCTGCTCGACGAGATCTCGGAGGGCCTTGCGCCGGTGATCGTGCAGGCGCTCGCGCGCATGATCCTGATGCT
CAAGAAGAAGGGCTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTCGCCGCGCCGCTGGCGGACCGCTTTTATGTGA
TGGAGCACGGCGGCATCGTCGAGCGCTTCGCGGCCAGCGAGCTGCAGGCCAAGATGCCGGTGCTCAATGAATTGCTGGGG
GTCTGA

Upstream 100 bases:

>100_bases
GGTCCGTACGCGGAAGTCTCGAAGGATCCGCGTGTGATGGAGGCTTACATGGGCACCGCGGACGCCGAGTTGCAGGGCGC
GCACTGAGGAGGCCATCGAC

Downstream 100 bases:

>100_bases
TCCGCGTATCCGCTTCGCACGGATCGCCAGATCGGTTATTTGGCAGGAACGGGTGGCGCGCTGGCGCTACAACACAGGAG
ACAACGCAATGAAGAAGACT

Product: ABC transporter related

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MTTSNVPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETIGLPTHKI
AHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADSSKGMSEAEIYDMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGA
NLLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGGIVERFAASELQAKMPVLNELLG
V

Sequences:

>Translated_241_residues
MTTSNVPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETIGLPTHKI
AHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADSSKGMSEAEIYDMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGA
NLLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGGIVERFAASELQAKMPVLNELLG
V
>Mature_240_residues
TTSNVPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGARKGSIKVNGHETIGLPTHKIA
HYGIGYCPEERAIFSSLSCEENLMLPPVLKGADSSKGMSEAEIYDMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGAN
LLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGGIVERFAASELQAKMPVLNELLGV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27881501, Length=204, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI30795238, Length=204, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI47078218, Length=198, Percent_Identity=30.8080808080808, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45446740, Length=198, Percent_Identity=30.8080808080808, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI31657092, Length=203, Percent_Identity=26.1083743842365, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI116734710, Length=191, Percent_Identity=30.3664921465969, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI21536376, Length=198, Percent_Identity=26.2626262626263, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI105990541, Length=191, Percent_Identity=26.7015706806283, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4505771, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI9961250, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI9961252, Length=240, Percent_Identity=27.5, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI150417984, Length=189, Percent_Identity=29.6296296296296, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27262624, Length=194, Percent_Identity=26.2886597938144, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI27262626, Length=194, Percent_Identity=26.2886597938144, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI42741659, Length=244, Percent_Identity=26.2295081967213, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI9955963, Length=221, Percent_Identity=27.1493212669683, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI153792144, Length=211, Percent_Identity=26.5402843601896, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI27436953, Length=194, Percent_Identity=27.8350515463918, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI27477115, Length=218, Percent_Identity=27.9816513761468, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21536378, Length=225, Percent_Identity=23.1111111111111, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI255708477, Length=215, Percent_Identity=27.4418604651163, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI148612844, Length=234, Percent_Identity=26.4957264957265, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI87082268, Length=232, Percent_Identity=40.5172413793103, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1789593, Length=222, Percent_Identity=32.8828828828829, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1789672, Length=229, Percent_Identity=29.2576419213974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=33.1896551724138, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787029, Length=216, Percent_Identity=28.7037037037037, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1787792, Length=245, Percent_Identity=29.7959183673469, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=30.9859154929577, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787105, Length=228, Percent_Identity=26.7543859649123, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787089, Length=225, Percent_Identity=27.5555555555556, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789864, Length=251, Percent_Identity=24.7011952191235, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788644, Length=235, Percent_Identity=30.6382978723404, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788450, Length=221, Percent_Identity=28.5067873303167, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI87081791, Length=218, Percent_Identity=28.4403669724771, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788761, Length=229, Percent_Identity=27.0742358078603, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790525, Length=226, Percent_Identity=26.9911504424779, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787370, Length=217, Percent_Identity=27.6497695852535, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786703, Length=211, Percent_Identity=28.436018957346, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786253, Length=237, Percent_Identity=29.957805907173, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=26.8518518518519, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=227, Percent_Identity=27.3127753303965, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787712, Length=216, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786398, Length=223, Percent_Identity=24.2152466367713, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790162, Length=238, Percent_Identity=25.2100840336134, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790190, Length=225, Percent_Identity=27.1111111111111, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787112, Length=238, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081709, Length=226, Percent_Identity=27.8761061946903, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1790467, Length=219, Percent_Identity=26.4840182648402, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786872, Length=217, Percent_Identity=27.6497695852535, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1789991, Length=229, Percent_Identity=27.0742358078603, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081782, Length=227, Percent_Identity=27.3127753303965, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994943, Length=230, Percent_Identity=24.3478260869565, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788165, Length=214, Percent_Identity=26.1682242990654, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788210, Length=235, Percent_Identity=28.5106382978723, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789873, Length=210, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082267, Length=214, Percent_Identity=27.1028037383178, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788472, Length=226, Percent_Identity=24.7787610619469, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=195, Percent_Identity=31.7948717948718, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=221, Percent_Identity=30.316742081448, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=200, Percent_Identity=30, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=200, Percent_Identity=30, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=200, Percent_Identity=30, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17565586, Length=220, Percent_Identity=27.7272727272727, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25146777, Length=213, Percent_Identity=26.7605633802817, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193211017, Length=240, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI193211015, Length=240, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71996809, Length=240, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI212646699, Length=226, Percent_Identity=26.9911504424779, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=224, Percent_Identity=26.7857142857143, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=238, Percent_Identity=24.3697478991597, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322640, Length=223, Percent_Identity=26.0089686098655, Blast_Score=62, Evalue=1e-10,
Organism=Drosophila melanogaster, GI24648314, Length=212, Percent_Identity=28.3018867924528, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=28.7735849056604, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI221512771, Length=213, Percent_Identity=28.169014084507, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI116007328, Length=212, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28574150, Length=212, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643674, Length=210, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573571, Length=230, Percent_Identity=24.7826086956522, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28573573, Length=230, Percent_Identity=24.7826086956522, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650855, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650853, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281362751, Length=203, Percent_Identity=26.6009852216749, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI85725262, Length=210, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357907, Length=220, Percent_Identity=27.7272727272727, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582014, Length=219, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24582012, Length=219, Percent_Identity=24.6575342465753, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363262, Length=226, Percent_Identity=24.7787610619469, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28571209, Length=218, Percent_Identity=22.4770642201835, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24653245, Length=226, Percent_Identity=24.7787610619469, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI45550390, Length=219, Percent_Identity=24.2009132420091, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26329; Mature: 26198

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSNVPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGA
CCCCCCCEEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCC
RKGSIKVNGHETIGLPTHKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADSSKGMS
CCCCEEECCCEEECCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCCCCC
EAEIYDMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVIVQAL
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHH
ARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGGIVERFAASELQAKMPVLNELLG
HHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure 
TTSNVPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLTGA
CCCCCCEEEEEHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCCC
RKGSIKVNGHETIGLPTHKIAHYGIGYCPEERAIFSSLSCEENLMLPPVLKGADSSKGMS
CCCCEEECCCEEECCCHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHCCCCCCCCCC
EAEIYDMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVIVQAL
HHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHH
ARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGGIVERFAASELQAKMPVLNELLG
HHHHHHHHCCCCEEEEEECCCEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]