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The map label for this gene is ampR [H]

Identifier: 73543042

GI number: 73543042

Start: 3678315

End: 3679226

Strand: Direct

Name: ampR [H]

Synonym: Reut_A3360

Alternate gene names: 73543042

Gene position: 3678315-3679226 (Clockwise)

Preceding gene: 73543017

Following gene: 73543055

Centisome position: 96.63

GC content: 65.35

Gene sequence:

>912_bases
ATGCGATTGCATTCCCCTTCAATGTCCGAGCTGCACGCCTTCGTCGCCGCCGCGCGCTTGGGCAGTTTCACCCGCGCGGC
CGACACCCTATGCGTGACACAGGGGGCTATCAGCCGGGCCATTGCCCGGCTCGAGGAACACTTTGGCCAGCCGCTGATTC
AGCGCAATGCGCACCGGTTGACACTGACGGGTGCCGGACAGCAGTTGCTGGACGCTGTCGCGGGCCCGCTTGCCGCGATC
GAGCACGCGAGTGCGGCATTGCGTGCCGGTGACCGTAGCCACCATCTGACGCTTTCTGCCGTGCCAACGCTTGCCAGTGT
CTGGCTGGTGCCCCGCCTGCCCGACTTCCACCGTCGACACCCCGATATCCGCCTCGATTTCGTGCCCTACCGCCGCGACG
AGGATTTCTCCGGTGCTGCGCCCGATGCTGCTTTACTGGCCGGCGAGCCGGGCAAATGGCCTGGCCTCCAGGTGGACTAT
GTAGTGGGCCGCGAGATGGTGCCGGTCTGCCATCCCGACCGCGCACGCCAACGGCATGCAGCCGGACGCTGGCGCGAGCC
CGCCGAGCTGATGGATGAACCCCTGCTCTATCACACCACCGCCCCTGCAAACTGGGCCAACTGGCTGCAGGCAGCAGGCG
TACCCAACGCTGCGCCGAAGCTGTCATCAGGCTTCGACCAGGTGTCGATCCTGGTACAGGCCGTGCGCGCCGACATGGGT
ATTGCGGTGCTGCAGCGCTGTCTGTTGCACGACGAACTAGCTGCCGGGCGGGTCGTAGCGCCGTTCGATCTTCCGATTCG
GCTGCAACGCGGCTATTTCCTGTGTTCACCAAGGGAGCGGCGCGATCATCACGCCCTGCGTTGCTTCCGCGAATGGCTGC
TGGAAACTGCCGCGCAGGATTCTGGGACATAA

Upstream 100 bases:

>100_bases
GGTTGTAAATTGCCGCCCGAGTGTCAGCCGTTTACCGGTCCGGGGCAAACGACTATATTGCACTTTTGCATTACCAAAGG
TCATCCTCGACCTCCGGCCC

Downstream 100 bases:

>100_bases
GCGACCCAGACTATTCGTCAGAAAGATGATCGCCTGACACTTAACACAGCTAATATCGATAAATCGAATAAACCTGTTGA
TCGCACGGATGAATCATTCA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRLHSPSMSELHAFVAAARLGSFTRAADTLCVTQGAISRAIARLEEHFGQPLIQRNAHRLTLTGAGQQLLDAVAGPLAAI
EHASAALRAGDRSHHLTLSAVPTLASVWLVPRLPDFHRRHPDIRLDFVPYRRDEDFSGAAPDAALLAGEPGKWPGLQVDY
VVGREMVPVCHPDRARQRHAAGRWREPAELMDEPLLYHTTAPANWANWLQAAGVPNAAPKLSSGFDQVSILVQAVRADMG
IAVLQRCLLHDELAAGRVVAPFDLPIRLQRGYFLCSPRERRDHHALRCFREWLLETAAQDSGT

Sequences:

>Translated_303_residues
MRLHSPSMSELHAFVAAARLGSFTRAADTLCVTQGAISRAIARLEEHFGQPLIQRNAHRLTLTGAGQQLLDAVAGPLAAI
EHASAALRAGDRSHHLTLSAVPTLASVWLVPRLPDFHRRHPDIRLDFVPYRRDEDFSGAAPDAALLAGEPGKWPGLQVDY
VVGREMVPVCHPDRARQRHAAGRWREPAELMDEPLLYHTTAPANWANWLQAAGVPNAAPKLSSGFDQVSILVQAVRADMG
IAVLQRCLLHDELAAGRVVAPFDLPIRLQRGYFLCSPRERRDHHALRCFREWLLETAAQDSGT
>Mature_303_residues
MRLHSPSMSELHAFVAAARLGSFTRAADTLCVTQGAISRAIARLEEHFGQPLIQRNAHRLTLTGAGQQLLDAVAGPLAAI
EHASAALRAGDRSHHLTLSAVPTLASVWLVPRLPDFHRRHPDIRLDFVPYRRDEDFSGAAPDAALLAGEPGKWPGLQVDY
VVGREMVPVCHPDRARQRHAAGRWREPAELMDEPLLYHTTAPANWANWLQAAGVPNAAPKLSSGFDQVSILVQAVRADMG
IAVLQRCLLHDELAAGRVVAPFDLPIRLQRGYFLCSPRERRDHHALRCFREWLLETAAQDSGT

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=30.1694915254237, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=283, Percent_Identity=30.3886925795053, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=29.553264604811, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI145693193, Length=308, Percent_Identity=25.3246753246753, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI157672245, Length=129, Percent_Identity=35.6589147286822, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788887, Length=262, Percent_Identity=29.0076335877863, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=272, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788748, Length=180, Percent_Identity=29.4444444444444, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33375; Mature: 33375

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLHSPSMSELHAFVAAARLGSFTRAADTLCVTQGAISRAIARLEEHFGQPLIQRNAHRL
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
TLTGAGQQLLDAVAGPLAAIEHASAALRAGDRSHHLTLSAVPTLASVWLVPRLPDFHRRH
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHCC
PDIRLDFVPYRRDEDFSGAAPDAALLAGEPGKWPGLQVDYVVGREMVPVCHPDRARQRHA
CCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AGRWREPAELMDEPLLYHTTAPANWANWLQAAGVPNAAPKLSSGFDQVSILVQAVRADMG
CCCCCCCHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
IAVLQRCLLHDELAAGRVVAPFDLPIRLQRGYFLCSPRERRDHHALRCFREWLLETAAQD
HHHHHHHHHHHHHHCCCEECCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
SGT
CCC
>Mature Secondary Structure
MRLHSPSMSELHAFVAAARLGSFTRAADTLCVTQGAISRAIARLEEHFGQPLIQRNAHRL
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEE
TLTGAGQQLLDAVAGPLAAIEHASAALRAGDRSHHLTLSAVPTLASVWLVPRLPDFHRRH
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHCC
PDIRLDFVPYRRDEDFSGAAPDAALLAGEPGKWPGLQVDYVVGREMVPVCHPDRARQRHA
CCEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHH
AGRWREPAELMDEPLLYHTTAPANWANWLQAAGVPNAAPKLSSGFDQVSILVQAVRADMG
CCCCCCCHHHHCCCCEEEECCCCCHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
IAVLQRCLLHDELAAGRVVAPFDLPIRLQRGYFLCSPRERRDHHALRCFREWLLETAAQD
HHHHHHHHHHHHHHCCCEECCCCCCEEEECCEEEECCHHHHHHHHHHHHHHHHHHHHHCC
SGT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8064842 [H]