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The map label for this gene is lip2 [H]

Identifier: 73543025

GI number: 73543025

Start: 3661085

End: 3662041

Strand: Reverse

Name: lip2 [H]

Synonym: Reut_A3343

Alternate gene names: 73543025

Gene position: 3662041-3661085 (Counterclockwise)

Preceding gene: 73543026

Following gene: 73543024

Centisome position: 96.2

GC content: 66.67

Gene sequence:

>957_bases
ATGGCTATCGATCCGCAACTCCTCGCCTATTACCGCCGCCTGGCCGAGCAATATGGCGCCCTTCCCGTACCCGTTGATGC
GCGCGCGCGGCGCGAGCGCTTTGCCGAAGTGGCAGCTTCGTCGGTCGTACCTGATCCTGCCGGCACCACGTGGGAAGACC
TCACCCTTGCGCTGGAAGGCCGCTCGCTGACGGCGCGCCTGTTCCGTCCGGTGGCCGATGCGCCGTCCCGGTTGATCGTT
TATTTCCACGGCGGCGGCTGGGTCGTCGGATCGCACGAGACCCACCATACCGTCGCAGCACTGCTGGCGGCTGATACGGG
CTGCGCGGTAGCCAGCGTGGATTATCGGCTCGCGCCTGAGTATGCGTTTCCCGCGCCATGCGATGACGCCGTAGATGCTG
TCCGTTGGTTCGCCGTGCAGCGCGCGTCGCTGGGCTTCGATACCGGCTTCCTTGCCGTGGCGGGCGATAGCGCTGGTGGA
CACCTCGCAGCACTCGCGGCGCAGCAGGTCAACACCGAAGCCCGACAGCAAGTGGTGGTGGCGCAGTTGCTCGTCTATCC
GGTCACAGCGCCGGTGCTTTCCAGCGAGAGCTACCGCGCCTTTGCCGATGGCCCCGGGCTGACCCGTGACGAGATGGCCT
GGTTCTGGACGCAGTTTATCGGCGCCGAGAGTCTGGCGCGCGGCGCGCAACACGAAGATCTACGCATTCATCTGATGGCG
CAGGCGCCGAAGGTGCAGCCGCCGGCCACCGCCATCGTCGTTGCCGCCAACGACGTGTTGCGCGATGACGGGCTTGCCTA
TGCCGATTTCCTCGTCCGGCACGACGCCACGGTAGTCACCATCGAGGCCAGTGGCATGACGCACGGATTCGCACGCCTGC
AACCGGACGCGCCGCGCGCGCGCGAATGGATGCAACGTGCCGCGCAGGCCTTCGCGAGCCTGTTCGACGACGTCTAG

Upstream 100 bases:

>100_bases
CCAGGCCCGCGTAGAGGGCCTGCTCGCCGTGCAAGACTGCTGCAGCAAGATCGAGCTTACCCGCCAGCTCGCGCTGTCCT
GAACCCTTTCGCCTGACCCG

Downstream 100 bases:

>100_bases
CGGTCTGGTGCGCCGGCACTGTCGCATCAGGCGCCGGTGCGCGGCGGCCATCCCTTAGAATGGCGGTCTTACGAGGATGA
CCATGACTGCACTGCAGAAC

Product: lipolytic protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAIDPQLLAYYRRLAEQYGALPVPVDARARRERFAEVAASSVVPDPAGTTWEDLTLALEGRSLTARLFRPVADAPSRLIV
YFHGGGWVVGSHETHHTVAALLAADTGCAVASVDYRLAPEYAFPAPCDDAVDAVRWFAVQRASLGFDTGFLAVAGDSAGG
HLAALAAQQVNTEARQQVVVAQLLVYPVTAPVLSSESYRAFADGPGLTRDEMAWFWTQFIGAESLARGAQHEDLRIHLMA
QAPKVQPPATAIVVAANDVLRDDGLAYADFLVRHDATVVTIEASGMTHGFARLQPDAPRAREWMQRAAQAFASLFDDV

Sequences:

>Translated_318_residues
MAIDPQLLAYYRRLAEQYGALPVPVDARARRERFAEVAASSVVPDPAGTTWEDLTLALEGRSLTARLFRPVADAPSRLIV
YFHGGGWVVGSHETHHTVAALLAADTGCAVASVDYRLAPEYAFPAPCDDAVDAVRWFAVQRASLGFDTGFLAVAGDSAGG
HLAALAAQQVNTEARQQVVVAQLLVYPVTAPVLSSESYRAFADGPGLTRDEMAWFWTQFIGAESLARGAQHEDLRIHLMA
QAPKVQPPATAIVVAANDVLRDDGLAYADFLVRHDATVVTIEASGMTHGFARLQPDAPRAREWMQRAAQAFASLFDDV
>Mature_317_residues
AIDPQLLAYYRRLAEQYGALPVPVDARARRERFAEVAASSVVPDPAGTTWEDLTLALEGRSLTARLFRPVADAPSRLIVY
FHGGGWVVGSHETHHTVAALLAADTGCAVASVDYRLAPEYAFPAPCDDAVDAVRWFAVQRASLGFDTGFLAVAGDSAGGH
LAALAAQQVNTEARQQVVVAQLLVYPVTAPVLSSESYRAFADGPGLTRDEMAWFWTQFIGAESLARGAQHEDLRIHLMAQ
APKVQPPATAIVVAANDVLRDDGLAYADFLVRHDATVVTIEASGMTHGFARLQPDAPRAREWMQRAAQAFASLFDDV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=171, Percent_Identity=30.9941520467836, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI206597554, Length=284, Percent_Identity=28.5211267605634, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI157041239, Length=141, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI68051721, Length=167, Percent_Identity=30.5389221556886, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI61966717, Length=155, Percent_Identity=30.9677419354839, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI226423947, Length=175, Percent_Identity=29.7142857142857, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI21328446, Length=96, Percent_Identity=39.5833333333333, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1786682, Length=249, Percent_Identity=28.1124497991968, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=309, Percent_Identity=26.8608414239482, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI72001146, Length=135, Percent_Identity=32.5925925925926, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=165, Percent_Identity=29.0909090909091, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=305, Percent_Identity=21.6393442622951, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=89, Percent_Identity=38.2022471910112, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=89, Percent_Identity=38.2022471910112, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34267; Mature: 34136

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDPQLLAYYRRLAEQYGALPVPVDARARRERFAEVAASSVVPDPAGTTWEDLTLALEG
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEC
RSLTARLFRPVADAPSRLIVYFHGGGWVVGSHETHHTVAALLAADTGCAVASVDYRLAPE
CCHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCEEEEECEEECCC
YAFPAPCDDAVDAVRWFAVQRASLGFDTGFLAVAGDSAGGHLAALAAQQVNTEARQQVVV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHH
AQLLVYPVTAPVLSSESYRAFADGPGLTRDEMAWFWTQFIGAESLARGAQHEDLRIHLMA
HHHHHHHCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCEEEEEEE
QAPKVQPPATAIVVAANDVLRDDGLAYADFLVRHDATVVTIEASGMTHGFARLQPDAPRA
CCCCCCCCCCEEEEEECHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCCHHH
REWMQRAAQAFASLFDDV
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIDPQLLAYYRRLAEQYGALPVPVDARARRERFAEVAASSVVPDPAGTTWEDLTLALEG
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEEC
RSLTARLFRPVADAPSRLIVYFHGGGWVVGSHETHHTVAALLAADTGCAVASVDYRLAPE
CCHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCEEEEECEEECCC
YAFPAPCDDAVDAVRWFAVQRASLGFDTGFLAVAGDSAGGHLAALAAQQVNTEARQQVVV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHH
AQLLVYPVTAPVLSSESYRAFADGPGLTRDEMAWFWTQFIGAESLARGAQHEDLRIHLMA
HHHHHHHCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCHHHHHCCCCCCCEEEEEEE
QAPKVQPPATAIVVAANDVLRDDGLAYADFLVRHDATVVTIEASGMTHGFARLQPDAPRA
CCCCCCCCCCEEEEEECHHHHCCCCHHHHHHHHCCCEEEEEEECCCCCCHHCCCCCCHHH
REWMQRAAQAFASLFDDV
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]