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The map label for this gene is priA [H]

Identifier: 73543023

GI number: 73543023

Start: 3657094

End: 3659379

Strand: Reverse

Name: priA [H]

Synonym: Reut_A3341

Alternate gene names: 73543023

Gene position: 3659379-3657094 (Counterclockwise)

Preceding gene: 73543024

Following gene: 73543022

Centisome position: 96.13

GC content: 67.24

Gene sequence:

>2286_bases
ATGACGTTGCCAGACGACACCGAACCGCGTAGCGCAGGCATTGTCCGCGTGGCGCTTGATACGCCCGCGGACGACTGTTT
CGACTACCTCGCCGACGATGAAGTGGTGCCGGGCGATCTGGTGGTCGTACCGTTCGGACGTCGCAGCGTGGTCGGCGTTG
CCGTTGCGCGCGCCGCCGAGTCGGCCGTGCCGGCGGACCGGATCAAGGCAGTTTCCGCGCGGCTGGGCTGGCTGGCGCCG
CTAGCGCAGGAATGGCGCGATCTTGCGACGTTCGCCGCGCGCTATTACCAGCGCTGCCTTGGCGAGGTGATGCTGCCGTC
GCTGCCGCCGTCGCTGCGGGAGCCGGAGGGCTGGCCACGCCTTGGCCAGCGTGCACGGACCGAGGCGTACCGGGTCGTTG
CGGCACATATCCCTACGTTGCTCGAGTCGATACCTGGGCGTGCGCGATCGGTGCGGGCGCTTGCGCAGGCGCTGGCCGAC
CGCGCCGCCGACGGCGCAGTGCCCGAGCCGATGAGTCTGACCGAGGCGCGCGAATTATGCACCGCCGCGCCAGCAAGGCT
TGTTGAATGGGCCGCTGCAGGCTGGATCGAGGCGATCAGCGCCGACCGGACCTTGCTCGGCACCGCGCCCTCGCAGGCCC
CGTCGGTGGCGCCTGCGTTGCATGAAGAACAGCTTGCGGCGGTCGAAGCTATCGGCGCCGCTGCGTCCTTTGCTCCGTTC
CTGCTGCACGGGGTGACCGGCAGCGGCAAGACTGAGGTCTATCTGCATGCCATGGCCCAGGTACTCGCGCGCGATCCTCA
CACGCAGGTGCTGATGCTGGTGCCCGAGATCAACCTGACCCCGCAACTCCAGTCGCGCATTGCCGCGCGCTTTCCGCACC
TGCCGCTGGCCGTGCTGCACAGCGGGCTGGCTGACCAGGAACGCAGCCTGCAATGGCTCGCCGCCCATCGCGGCGAAGCG
CGCATCGTACTGGGCACGCGCATGGCGATCATGGCCTCGATGCCGAATCTTGCGCTAATCGTGGTCGATGAAGAGCACGA
CACGTCCTACAAACAGCAGGAGGGCCTGCGCTACTCCGCGCGCGACCTGGCCGTATGGCGCGCCAAGCGGCTCGAAATTC
CGGTCGTACTTGGCTCGGCCACGCCGTCAATGGAAACCTGGTGGCGCGCTGAGCAGGGCGCGTATCGCAAGCTGGTGCTG
CGCCAGCGCGCGCAGGCTGAAGCGGCGCTGCCGGCCGTGCGTCTGATCGATCTGAACCATGAACGCCAGCGCCAGCGGGC
GCTGTTCGAAGGCCTGTCGGTGCCGTTGCTGGAGGCGATCGACGCCCGGCTGGCTCGCGGGGAGCAGAGCCTGCTGTTCC
TGAACCGACGTGGCTATGCGCCGGTGCTGGCCTGCGATAGCTGCGGCTGGCTGTCTGGCTGCCCGCGCTGTTCGGCATAC
CTGGTGCTGCACCGGCCGGAACGGCGGCTGCGCTGCCACCACTGCGGGCTGGAGGCGGCCGTGCCGCACCATTGCCCTGA
CTGCGGCAATATGGATATCGCGCCGCTTGGCCGTGGCACCCAGCGCATCGAAGAAGCGCTGGCGGCACGTTTTCCACAGG
CCCGTATCGCTCGCATCGATGCCGATTCCACGCGGCGCAAAGGCAGTGCGCAGGCGATGTTCGACGAAGTGCATGCCGGC
GAGGTGGACATCCTCGTCGGTACCCAGATGATCGCCAAGGGCCATGATTTTCAACGGGTGACGCTGGTCGGCATCGTGCA
TCCGGACGCCGCCATGTTCAGCCACGATTTCCGCGCCGCCGAACACCTGTTCGCGTCGCTGATGCAGGTATCCGGGCGGG
CCGGGCGGGCCGGCCTGCCCGGCGAAGTCCTCATCCAGACCCGCTATCCAGACGCTGCGGCATTGCAGGCCCTCGTGCGC
CATGACTATGATGGATTCGCCGCCGGCCAGTTGCGCGAGCGCCGCCAGGCCGGGTTGCCCCCGTTCTGCTATCAGGTGCT
GCTGACTGCCGAGCATGGCCAGCTCGAACGGGCATTGCAGTTCCTGCAGGCGGTCCGGCGCCTGGCAGACGGCAATGCTT
TGGCGCCGGAGGTGCAACTGCACGATCCGGTACCGATGTCGATGGTGCGCATTGCTAACCGCGAACGTGCCCAGATGTTG
TTGGAAGCCAGCTCAAGGCCCGTTTTGCAACGATTTGTGAGCGAGTGGGCGCGGACTTTTTCCGATGCCGGCGCAGACGC
AAGAGGCGTGCGCTGGCAACTGGAAATCGACCCGCTGAGAATCTGA

Upstream 100 bases:

>100_bases
TTAAGTTGATGTTGCGCCGCACACTAAGCCTGCTTCTATTCCCTTTGCGCCTGAATCCGCACAGTCTTTGCGCACGCGCC
GTGCACCAGAACTGCCTCTG

Downstream 100 bases:

>100_bases
GTGCCGGGCTGCATGTGACTGGGGTGCAACCGTAGCAACCGGCTTTCGAGATAGTGGTTGCACTAGGTTGCACCTTTGCG
TTTTTCAGGAGTAAGATCGC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MTLPDDTEPRSAGIVRVALDTPADDCFDYLADDEVVPGDLVVVPFGRRSVVGVAVARAAESAVPADRIKAVSARLGWLAP
LAQEWRDLATFAARYYQRCLGEVMLPSLPPSLREPEGWPRLGQRARTEAYRVVAAHIPTLLESIPGRARSVRALAQALAD
RAADGAVPEPMSLTEARELCTAAPARLVEWAAAGWIEAISADRTLLGTAPSQAPSVAPALHEEQLAAVEAIGAAASFAPF
LLHGVTGSGKTEVYLHAMAQVLARDPHTQVLMLVPEINLTPQLQSRIAARFPHLPLAVLHSGLADQERSLQWLAAHRGEA
RIVLGTRMAIMASMPNLALIVVDEEHDTSYKQQEGLRYSARDLAVWRAKRLEIPVVLGSATPSMETWWRAEQGAYRKLVL
RQRAQAEAALPAVRLIDLNHERQRQRALFEGLSVPLLEAIDARLARGEQSLLFLNRRGYAPVLACDSCGWLSGCPRCSAY
LVLHRPERRLRCHHCGLEAAVPHHCPDCGNMDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMFDEVHAG
EVDILVGTQMIAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLPGEVLIQTRYPDAAALQALVR
HDYDGFAAGQLRERRQAGLPPFCYQVLLTAEHGQLERALQFLQAVRRLADGNALAPEVQLHDPVPMSMVRIANRERAQML
LEASSRPVLQRFVSEWARTFSDAGADARGVRWQLEIDPLRI

Sequences:

>Translated_761_residues
MTLPDDTEPRSAGIVRVALDTPADDCFDYLADDEVVPGDLVVVPFGRRSVVGVAVARAAESAVPADRIKAVSARLGWLAP
LAQEWRDLATFAARYYQRCLGEVMLPSLPPSLREPEGWPRLGQRARTEAYRVVAAHIPTLLESIPGRARSVRALAQALAD
RAADGAVPEPMSLTEARELCTAAPARLVEWAAAGWIEAISADRTLLGTAPSQAPSVAPALHEEQLAAVEAIGAAASFAPF
LLHGVTGSGKTEVYLHAMAQVLARDPHTQVLMLVPEINLTPQLQSRIAARFPHLPLAVLHSGLADQERSLQWLAAHRGEA
RIVLGTRMAIMASMPNLALIVVDEEHDTSYKQQEGLRYSARDLAVWRAKRLEIPVVLGSATPSMETWWRAEQGAYRKLVL
RQRAQAEAALPAVRLIDLNHERQRQRALFEGLSVPLLEAIDARLARGEQSLLFLNRRGYAPVLACDSCGWLSGCPRCSAY
LVLHRPERRLRCHHCGLEAAVPHHCPDCGNMDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMFDEVHAG
EVDILVGTQMIAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLPGEVLIQTRYPDAAALQALVR
HDYDGFAAGQLRERRQAGLPPFCYQVLLTAEHGQLERALQFLQAVRRLADGNALAPEVQLHDPVPMSMVRIANRERAQML
LEASSRPVLQRFVSEWARTFSDAGADARGVRWQLEIDPLRI
>Mature_760_residues
TLPDDTEPRSAGIVRVALDTPADDCFDYLADDEVVPGDLVVVPFGRRSVVGVAVARAAESAVPADRIKAVSARLGWLAPL
AQEWRDLATFAARYYQRCLGEVMLPSLPPSLREPEGWPRLGQRARTEAYRVVAAHIPTLLESIPGRARSVRALAQALADR
AADGAVPEPMSLTEARELCTAAPARLVEWAAAGWIEAISADRTLLGTAPSQAPSVAPALHEEQLAAVEAIGAAASFAPFL
LHGVTGSGKTEVYLHAMAQVLARDPHTQVLMLVPEINLTPQLQSRIAARFPHLPLAVLHSGLADQERSLQWLAAHRGEAR
IVLGTRMAIMASMPNLALIVVDEEHDTSYKQQEGLRYSARDLAVWRAKRLEIPVVLGSATPSMETWWRAEQGAYRKLVLR
QRAQAEAALPAVRLIDLNHERQRQRALFEGLSVPLLEAIDARLARGEQSLLFLNRRGYAPVLACDSCGWLSGCPRCSAYL
VLHRPERRLRCHHCGLEAAVPHHCPDCGNMDIAPLGRGTQRIEEALAARFPQARIARIDADSTRRKGSAQAMFDEVHAGE
VDILVGTQMIAKGHDFQRVTLVGIVHPDAAMFSHDFRAAEHLFASLMQVSGRAGRAGLPGEVLIQTRYPDAAALQALVRH
DYDGFAAGQLRERRQAGLPPFCYQVLLTAEHGQLERALQFLQAVRRLADGNALAPEVQLHDPVPMSMVRIANRERAQMLL
EASSRPVLQRFVSEWARTFSDAGADARGVRWQLEIDPLRI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=40.4479578392622, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 83414; Mature: 83282

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPDDTEPRSAGIVRVALDTPADDCFDYLADDEVVPGDLVVVPFGRRSVVGVAVARAAE
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
SAVPADRIKAVSARLGWLAPLAQEWRDLATFAARYYQRCLGEVMLPSLPPSLREPEGWPR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LGQRARTEAYRVVAAHIPTLLESIPGRARSVRALAQALADRAADGAVPEPMSLTEARELC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TAAPARLVEWAAAGWIEAISADRTLLGTAPSQAPSVAPALHEEQLAAVEAIGAAASFAPF
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLHGVTGSGKTEVYLHAMAQVLARDPHTQVLMLVPEINLTPQLQSRIAARFPHLPLAVLH
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHH
SGLADQERSLQWLAAHRGEARIVLGTRMAIMASMPNLALIVVDEEHDTSYKQQEGLRYSA
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCH
RDLAVWRAKRLEIPVVLGSATPSMETWWRAEQGAYRKLVLRQRAQAEAALPAVRLIDLNH
HHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
ERQRQRALFEGLSVPLLEAIDARLARGEQSLLFLNRRGYAPVLACDSCGWLSGCPRCSAY
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCEE
LVLHRPERRLRCHHCGLEAAVPHHCPDCGNMDIAPLGRGTQRIEEALAARFPQARIARID
EEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEC
ADSTRRKGSAQAMFDEVHAGEVDILVGTQMIAKGHDFQRVTLVGIVHPDAAMFSHDFRAA
CCHHHCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHH
EHLFASLMQVSGRAGRAGLPGEVLIQTRYPDAAALQALVRHDYDGFAAGQLRERRQAGLP
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PFCYQVLLTAEHGQLERALQFLQAVRRLADGNALAPEVQLHDPVPMSMVRIANRERAQML
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHH
LEASSRPVLQRFVSEWARTFSDAGADARGVRWQLEIDPLRI
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TLPDDTEPRSAGIVRVALDTPADDCFDYLADDEVVPGDLVVVPFGRRSVVGVAVARAAE
CCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHH
SAVPADRIKAVSARLGWLAPLAQEWRDLATFAARYYQRCLGEVMLPSLPPSLREPEGWPR
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LGQRARTEAYRVVAAHIPTLLESIPGRARSVRALAQALADRAADGAVPEPMSLTEARELC
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
TAAPARLVEWAAAGWIEAISADRTLLGTAPSQAPSVAPALHEEQLAAVEAIGAAASFAPF
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLHGVTGSGKTEVYLHAMAQVLARDPHTQVLMLVPEINLTPQLQSRIAARFPHLPLAVLH
HHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHH
SGLADQERSLQWLAAHRGEARIVLGTRMAIMASMPNLALIVVDEEHDTSYKQQEGLRYSA
CCCCCHHHHHHHHHHCCCCEEEEEECHHHHHHCCCCEEEEEEECCCCCCHHHHCCCCCCH
RDLAVWRAKRLEIPVVLGSATPSMETWWRAEQGAYRKLVLRQRAQAEAALPAVRLIDLNH
HHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
ERQRQRALFEGLSVPLLEAIDARLARGEQSLLFLNRRGYAPVLACDSCGWLSGCPRCSAY
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCCEE
LVLHRPERRLRCHHCGLEAAVPHHCPDCGNMDIAPLGRGTQRIEEALAARFPQARIARID
EEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEEEC
ADSTRRKGSAQAMFDEVHAGEVDILVGTQMIAKGHDFQRVTLVGIVHPDAAMFSHDFRAA
CCHHHCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHH
EHLFASLMQVSGRAGRAGLPGEVLIQTRYPDAAALQALVRHDYDGFAAGQLRERRQAGLP
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
PFCYQVLLTAEHGQLERALQFLQAVRRLADGNALAPEVQLHDPVPMSMVRIANRERAQML
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHH
LEASSRPVLQRFVSEWARTFSDAGADARGVRWQLEIDPLRI
HHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]