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The map label for this gene is livK [C]

Identifier: 73543021

GI number: 73543021

Start: 3651627

End: 3652778

Strand: Reverse

Name: livK [C]

Synonym: Reut_A3339

Alternate gene names: 73543021

Gene position: 3652778-3651627 (Counterclockwise)

Preceding gene: 73543022

Following gene: 73543020

Centisome position: 95.96

GC content: 64.93

Gene sequence:

>1152_bases
ATGACCTCGACATTCCACAAGACGGCCCTGACCGTTGCCCTGACTATCGCTGGCCTGACTGGCGCCACGGCGGCTTTCGC
CCAGGCCCAGGAAATCAAGCTCGGCTTTGCCGGCCCGATGACCGGCGGCCAGGCCCAGTACGGCAAGGACATGCAGAACG
GCATCGTCCTCGCCATCGAGGACATGAACGCCACCAAGCCGAAGATCGGCGGCAAGGAAGTCAAGTTCGTGCTGGTCTCC
GAAGACGACCAGGCCGACCCGAAGACCGGCTCGGTGGTCGCGCAGAAGCTCGTCGACAACGGCATCCAAGGCATGCTCGA
CCACTTCAACTCGGGCACCACCATCCCGGCGTCGATGGTCTACAACCGCGCAGGCATTCCGCAGATCGCCATGGCGACCG
CACCGGAATACACCAAGCAAGGCTTCAAGACGACCTTCCGCATGATGACTTCCGACACCCAGCAGGGTTCGGTGATCGGC
GCGTTCGTCGTGAAGAAGCTGGGCGCGAAGAACATCGCGATCGTTGACGACCGCACCGCGTACGGCCAGGGCCTGGCTGA
CGAGTTCGAGAAGGCCGCCAAGGCCGCCGGCGGCAAGATCGTGCGCCGCGAGTTCACCAACGACAAGGCGGTGGACTTCA
AGGCCGTGCTGACCAACATCAAGCGCAGCAACCCGGACATCATCTTCTACGGCGGCGCCGAGACCCAGTCGGCCCCGATG
GCCAAGCAGGTCAAGGAACTGGGCATGAAGGCCCCGCTGGTGTCGGGCGAAATGTCCAAGACCGACAACTTCCTGAAGCT
GGCCGGCCCGGCTGCCGAAGGCACGGTCGTGTCGCTGGCCGGCCTGCCGCTGGACCAGATGCCGGGCGGCGCCGCGTACG
AGAAGAAGTACGAGAAGCGCTTCGGCTCGAAGGTGCAGACCTACTCGCCGTACGCCTATGACGGCGCCACCGCGATGATG
ACCGCCATGGTCAAGGCCGGCTCGGCCGATCCGGCGCGCTATCTGCCGGTGCTGGCGGCGACCAACATGCAGGGCGTCAC
CACCAAGACGCTGGCGTACGACGCCCGTGGCGACCTGAAGGACGGCGGCATCACCGTCTACAAGGTGGTCGGCGGCAAGT
GGACCGTGCTGGAGACGGTTGGCGGCAAGTAA

Upstream 100 bases:

>100_bases
TGCCGCCATGCGCATCCATAGGGGAGGAGCGCCCAGACGGCGTTCCCGAAGCGTAGAAAAAGCGCAGCAAGAAGGCTGAA
CCAGCCACCAAGGAGAACTG

Downstream 100 bases:

>100_bases
GCCCACCGGCGCCAGGCCAGAAGAACGCCACCCTGCGAGGGTGGCGTTTTTTCTTGCCAATCCTAAAGATTGAACTGACC
CCTGCCGTTTGTTGTCCCGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTSTFHKTALTVALTIAGLTGATAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIEDMNATKPKIGGKEVKFVLVS
EDDQADPKTGSVVAQKLVDNGIQGMLDHFNSGTTIPASMVYNRAGIPQIAMATAPEYTKQGFKTTFRMMTSDTQQGSVIG
AFVVKKLGAKNIAIVDDRTAYGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRSNPDIIFYGGAETQSAPM
AKQVKELGMKAPLVSGEMSKTDNFLKLAGPAAEGTVVSLAGLPLDQMPGGAAYEKKYEKRFGSKVQTYSPYAYDGATAMM
TAMVKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLKDGGITVYKVVGGKWTVLETVGGK

Sequences:

>Translated_383_residues
MTSTFHKTALTVALTIAGLTGATAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIEDMNATKPKIGGKEVKFVLVS
EDDQADPKTGSVVAQKLVDNGIQGMLDHFNSGTTIPASMVYNRAGIPQIAMATAPEYTKQGFKTTFRMMTSDTQQGSVIG
AFVVKKLGAKNIAIVDDRTAYGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRSNPDIIFYGGAETQSAPM
AKQVKELGMKAPLVSGEMSKTDNFLKLAGPAAEGTVVSLAGLPLDQMPGGAAYEKKYEKRFGSKVQTYSPYAYDGATAMM
TAMVKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLKDGGITVYKVVGGKWTVLETVGGK
>Mature_382_residues
TSTFHKTALTVALTIAGLTGATAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIEDMNATKPKIGGKEVKFVLVSE
DDQADPKTGSVVAQKLVDNGIQGMLDHFNSGTTIPASMVYNRAGIPQIAMATAPEYTKQGFKTTFRMMTSDTQQGSVIGA
FVVKKLGAKNIAIVDDRTAYGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRSNPDIIFYGGAETQSAPMA
KQVKELGMKAPLVSGEMSKTDNFLKLAGPAAEGTVVSLAGLPLDQMPGGAAYEKKYEKRFGSKVQTYSPYAYDGATAMMT
AMVKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLKDGGITVYKVVGGKWTVLETVGGK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=350, Percent_Identity=33.4285714285714, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI48994941, Length=353, Percent_Identity=30.8781869688385, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40378; Mature: 40247

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTFHKTALTVALTIAGLTGATAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIE
CCCCHHHHHHEEEHEECCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEE
DMNATKPKIGGKEVKFVLVSEDDQADPKTGSVVAQKLVDNGIQGMLDHFNSGTTIPASMV
CCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YNRAGIPQIAMATAPEYTKQGFKTTFRMMTSDTQQGSVIGAFVVKKLGAKNIAIVDDRTA
HCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCH
YGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRSNPDIIFYGGAETQSAPM
HCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHH
AKQVKELGMKAPLVSGEMSKTDNFLKLAGPAAEGTVVSLAGLPLDQMPGGAAYEKKYEKR
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHCCCCHHHHHHHHHH
FGSKVQTYSPYAYDGATAMMTAMVKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLK
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCEEEEEEECCCCCCC
DGGITVYKVVGGKWTVLETVGGK
CCCEEEEEEECCEEEEEEECCCC
>Mature Secondary Structure 
TSTFHKTALTVALTIAGLTGATAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIE
CCCHHHHHHEEEHEECCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEE
DMNATKPKIGGKEVKFVLVSEDDQADPKTGSVVAQKLVDNGIQGMLDHFNSGTTIPASMV
CCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
YNRAGIPQIAMATAPEYTKQGFKTTFRMMTSDTQQGSVIGAFVVKKLGAKNIAIVDDRTA
HCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCH
YGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRSNPDIIFYGGAETQSAPM
HCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHH
AKQVKELGMKAPLVSGEMSKTDNFLKLAGPAAEGTVVSLAGLPLDQMPGGAAYEKKYEKR
HHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCHHHCCCCHHHHHHHHHH
FGSKVQTYSPYAYDGATAMMTAMVKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLK
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHEEEECCCCCCEEEEEEECCCCCCC
DGGITVYKVVGGKWTVLETVGGK
CCCEEEEEEECCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA