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The map label for this gene is hemB [H]

Identifier: 73542833

GI number: 73542833

Start: 3460483

End: 3461475

Strand: Direct

Name: hemB [H]

Synonym: Reut_A3149

Alternate gene names: 73542833

Gene position: 3460483-3461475 (Clockwise)

Preceding gene: 73542832

Following gene: 73542834

Centisome position: 90.91

GC content: 65.86

Gene sequence:

>993_bases
ATGTCTGCCTTTCCCGAGTACCGTCCCCGCCGCATGCGTCGCGATGACTTCTCGCGGCGTCTGATGCGCGAAAACCGTTT
GTCGCCGGACAACCTGATCTATCCCGTCTTCATCCTGGACGGGCAGAACCAGCGCCAGACGGTGGCATCCATGCCCGGCG
TGGAGCGCGTGTCGGTCGACCTGCTGCTGCCGGTGGCCGAAGACTGCGTGAAACTCGGCATCCCGGTGATCGCGCTGTTC
CCGGTCATCGACCCGTCGCTGAAGACGCCCGACGGCATCGAGGCGACCAATGCCGACGGCCTGGTGCCGCGCGCCGTGCG
TGCGCTGAAGGACCGCTTCCCGGAACTCGGCGTGCTGACCGACGTGGCACTCGACCCGTACACGAGCCATGGCCAGGACG
GCGTGCTTGATGACAACGGCTACGTGATCAACGACCAGACTGTCGAGATCCTGGTCAGGCAAGCACTGGTCCAGGCCGAG
GCCGGCGTCGACATCGTCGCGCCGTCCGACATGATGGATGGCCGCATCGGCGCCGTGCGCATGGCGCTGGAAGACGCGGG
CCACATCCATACGCGCATCATGGCGTACTCGGCCAAGTACGCGTCAGCGTTCTACGGCCCGTTCCGCGACGCCGTCGGCT
CGGCTGCCAATCTGGGCAAAGGCAACAAGATGACCTACCAGATGGACCCGGCCAACACCGATGAAGCCCTGCGCGAAGTC
GCCCAGGACATCATGGAAGGCGCCGACATGGTGATGGTCAAGCCCGGCATGCCGTACCTGGACATCGTGCGCCGCGTGAA
GGACGAGTTCCGCTTCCCGACCTACGTCTACCAGGTCAGCGGCGAGTATGCGATGCTCAAGGCCGCCGCGCAGAACGGCT
GGCTCGACCATGACAAGGTCATGATGGAATCGCTGCTGGCGTTCCGCCGCGCCGGCGCCGACGGCATCCTGACCTACTTC
GCGCGCGACGCCGCGCGCCTGCTCGCCGGCTAA

Upstream 100 bases:

>100_bases
TAGTTTTTCTGCGGAATGCGCTACGGCGTGCCCGCCGCGGGTCGGTGGTGAACCATCTTCCGCACATTTCCTCCTGTGAA
TTTCCCTCCTTCAAGCTGCC

Downstream 100 bases:

>100_bases
GGCCACGCCGCACGCATGGAGTTGCTAGGCTTTTCAGCCGGCCAGGTTCACCCGCTCGCCTCCCTGGATGAGGCGGCGGC
GTTCCTCGGCGGCAATGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSAFPEYRPRRMRRDDFSRRLMRENRLSPDNLIYPVFILDGQNQRQTVASMPGVERVSVDLLLPVAEDCVKLGIPVIALF
PVIDPSLKTPDGIEATNADGLVPRAVRALKDRFPELGVLTDVALDPYTSHGQDGVLDDNGYVINDQTVEILVRQALVQAE
AGVDIVAPSDMMDGRIGAVRMALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANTDEALREV
AQDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFRRAGADGILTYF
ARDAARLLAG

Sequences:

>Translated_330_residues
MSAFPEYRPRRMRRDDFSRRLMRENRLSPDNLIYPVFILDGQNQRQTVASMPGVERVSVDLLLPVAEDCVKLGIPVIALF
PVIDPSLKTPDGIEATNADGLVPRAVRALKDRFPELGVLTDVALDPYTSHGQDGVLDDNGYVINDQTVEILVRQALVQAE
AGVDIVAPSDMMDGRIGAVRMALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANTDEALREV
AQDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFRRAGADGILTYF
ARDAARLLAG
>Mature_329_residues
SAFPEYRPRRMRRDDFSRRLMRENRLSPDNLIYPVFILDGQNQRQTVASMPGVERVSVDLLLPVAEDCVKLGIPVIALFP
VIDPSLKTPDGIEATNADGLVPRAVRALKDRFPELGVLTDVALDPYTSHGQDGVLDDNGYVINDQTVEILVRQALVQAEA
GVDIVAPSDMMDGRIGAVRMALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANTDEALREVA
QDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFRRAGADGILTYFA
RDAARLLAG

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=296, Percent_Identity=45.2702702702703, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=45.5974842767296, Blast_Score=248, Evalue=3e-67,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=39.5424836601307, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI21358291, Length=304, Percent_Identity=43.421052631579, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36493; Mature: 36362

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFPEYRPRRMRRDDFSRRLMRENRLSPDNLIYPVFILDGQNQRQTVASMPGVERVSVD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCEEEHE
LLLPVAEDCVKLGIPVIALFPVIDPSLKTPDGIEATNADGLVPRAVRALKDRFPELGVLT
EEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DVALDPYTSHGQDGVLDDNGYVINDQTVEILVRQALVQAEAGVDIVAPSDMMDGRIGAVR
HHCCCCCCCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHCCCEEECCCHHCCCCHHHHH
MALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANTDEALREV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHHH
AQDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKV
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
MMESLLAFRRAGADGILTYFARDAARLLAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAFPEYRPRRMRRDDFSRRLMRENRLSPDNLIYPVFILDGQNQRQTVASMPGVERVSVD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCHHHHHHCCCCCEEEHE
LLLPVAEDCVKLGIPVIALFPVIDPSLKTPDGIEATNADGLVPRAVRALKDRFPELGVLT
EEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHH
DVALDPYTSHGQDGVLDDNGYVINDQTVEILVRQALVQAEAGVDIVAPSDMMDGRIGAVR
HHCCCCCCCCCCCCEECCCCEEECCHHHHHHHHHHHHHHHCCCEEECCCHHCCCCHHHHH
MALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSAANLGKGNKMTYQMDPANTDEALREV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEECCCCHHHHHHHH
AQDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKV
HHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCHHHH
MMESLLAFRRAGADGILTYFARDAARLLAG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]