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The map label for this gene is hemK [H]

Identifier: 73542730

GI number: 73542730

Start: 3350143

End: 3351012

Strand: Direct

Name: hemK [H]

Synonym: Reut_A3046

Alternate gene names: 73542730

Gene position: 3350143-3351012 (Clockwise)

Preceding gene: 73542729

Following gene: 73542731

Centisome position: 88.01

GC content: 69.31

Gene sequence:

>870_bases
ATGTCTCCTGCCATGTCCGGCGCCTTGCCGGCTACTCCCACCGTGCGTGAAGCGCAGTCGCTCGCCGCCATGGCCGGCCT
GCCGACGCTGGAAGCGCGGATGCTGCTGACCCACGTCACGGGCCTGACCCGCACCCAGCTGATCACGCGTGACGGCGACA
CCCTGACCCTGCCGCAGCGCGATGCCTTTGCCACGCTGCTGGCGCGGCGCTTGGCCGGCGAGCCGATGGCCTACCTGATC
GGCGAGCGCGAGTTCTTCGGCCGCAAGTTCCGCGTCACGCCCGATGTGCTGATCCCGCGCCCCGACACGGAAATCGCCGC
CGAATCGTCGCTCGCGCGGCTGGCCGGCGTGCCGCATCCGCGCGTGCTCGATTTGGGCACGGGCTCGGGCATCCTGGCCG
TGACCATTGCACGCGAACGGACCGATGCCGAAGTCTGGGCCACCGATATCTCGCCCGGCGCGCTGATGGTGGCGCAGGAC
AATGCCAGCGCGCTTGGCGCCAACCGCATCCATTTCCTGGTGTCGGACTGGTATGCCGCGCTGCCGGCCGAACTGCATTT
CCACCTGATCGTCAGCAACCCGCCCTATATCGCCGAAGGCGATCCGCATCTGATCGAGGGTGACCTGCGGTTCGAACCGA
TCGACGCGCTTACCGATCACAAAGACGGACTGTCCGACCTGGCGGCGATCGTCGCCGGCGCGCACGGGCGGCTGCTGCCA
GGCGGCTGGCTGCTGATGGAGCATGGCTACGACCAGGCCGAGGCCACACAAGGGCTGCTGGAGCGTGCCGGCTTCACCGA
GGTCTTCACCGCACGGGACCTCGCCGGACAGCAGCGCTGCACTGGAGGGCGCCGCCGGCCGGCCGCCTGA

Upstream 100 bases:

>100_bases
CTGCCAGACCCGCCGAAAGCCCCGCCAGGCCAACGCCCGGCGGGGTTTTTTGTTTTTTGGCTGGCGGTTTGCCGCCGGCG
TATCCGTCCCCGCCTTGCCG

Downstream 100 bases:

>100_bases
AGGCTGGCGGCGATTCCGGTAAAATTTGCAGCCAGATCACCACCGCACGGTCACCGTGCGGCCTTCGACACCCGGTGGCG
CCTGACGGTCAGGTGCCCCT

Product: modification methylase HemK

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSPAMSGALPATPTVREAQSLAAMAGLPTLEARMLLTHVTGLTRTQLITRDGDTLTLPQRDAFATLLARRLAGEPMAYLI
GEREFFGRKFRVTPDVLIPRPDTEIAAESSLARLAGVPHPRVLDLGTGSGILAVTIARERTDAEVWATDISPGALMVAQD
NASALGANRIHFLVSDWYAALPAELHFHLIVSNPPYIAEGDPHLIEGDLRFEPIDALTDHKDGLSDLAAIVAGAHGRLLP
GGWLLMEHGYDQAEATQGLLERAGFTEVFTARDLAGQQRCTGGRRRPAA

Sequences:

>Translated_289_residues
MSPAMSGALPATPTVREAQSLAAMAGLPTLEARMLLTHVTGLTRTQLITRDGDTLTLPQRDAFATLLARRLAGEPMAYLI
GEREFFGRKFRVTPDVLIPRPDTEIAAESSLARLAGVPHPRVLDLGTGSGILAVTIARERTDAEVWATDISPGALMVAQD
NASALGANRIHFLVSDWYAALPAELHFHLIVSNPPYIAEGDPHLIEGDLRFEPIDALTDHKDGLSDLAAIVAGAHGRLLP
GGWLLMEHGYDQAEATQGLLERAGFTEVFTARDLAGQQRCTGGRRRPAA
>Mature_288_residues
SPAMSGALPATPTVREAQSLAAMAGLPTLEARMLLTHVTGLTRTQLITRDGDTLTLPQRDAFATLLARRLAGEPMAYLIG
EREFFGRKFRVTPDVLIPRPDTEIAAESSLARLAGVPHPRVLDLGTGSGILAVTIARERTDAEVWATDISPGALMVAQDN
ASALGANRIHFLVSDWYAALPAELHFHLIVSNPPYIAEGDPHLIEGDLRFEPIDALTDHKDGLSDLAAIVAGAHGRLLPG
GWLLMEHGYDQAEATQGLLERAGFTEVFTARDLAGQQRCTGGRRRPAA

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=306, Percent_Identity=28.4313725490196, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=45.5555555555556, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082085, Length=199, Percent_Identity=34.6733668341709, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24582226, Length=199, Percent_Identity=30.6532663316583, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31005; Mature: 30874

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAMSGALPATPTVREAQSLAAMAGLPTLEARMLLTHVTGLTRTQLITRDGDTLTLPQR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEECCCCEEECCCH
DAFATLLARRLAGEPMAYLIGEREFFGRKFRVTPDVLIPRPDTEIAAESSLARLAGVPHP
HHHHHHHHHHHCCCCCEEEECCHHHHCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCC
RVLDLGTGSGILAVTIARERTDAEVWATDISPGALMVAQDNASALGANRIHFLVSDWYAA
EEEEECCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEEEHHHHHH
LPAELHFHLIVSNPPYIAEGDPHLIEGDLRFEPIDALTDHKDGLSDLAAIVAGAHGRLLP
CCCEEEEEEEECCCCEEECCCCCEEECCCEECCCHHHHCCCCCHHHHHHHHHCCCCCCCC
GGWLLMEHGYDQAEATQGLLERAGFTEVFTARDLAGQQRCTGGRRRPAA
CCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCCC
>Mature Secondary Structure 
SPAMSGALPATPTVREAQSLAAMAGLPTLEARMLLTHVTGLTRTQLITRDGDTLTLPQR
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEEEECCCCEEECCCH
DAFATLLARRLAGEPMAYLIGEREFFGRKFRVTPDVLIPRPDTEIAAESSLARLAGVPHP
HHHHHHHHHHHCCCCCEEEECCHHHHCCEEECCCCEEECCCCCHHHHHHHHHHHHCCCCC
RVLDLGTGSGILAVTIARERTDAEVWATDISPGALMVAQDNASALGANRIHFLVSDWYAA
EEEEECCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCCCCCCCEEEEEEHHHHHH
LPAELHFHLIVSNPPYIAEGDPHLIEGDLRFEPIDALTDHKDGLSDLAAIVAGAHGRLLP
CCCEEEEEEEECCCCEEECCCCCEEECCCEECCCHHHHCCCCCHHHHHHHHHCCCCCCCC
GGWLLMEHGYDQAEATQGLLERAGFTEVFTARDLAGQQRCTGGRRRPAA
CCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]