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The map label for this gene is prfA

Identifier: 73542728

GI number: 73542728

Start: 3348379

End: 3349461

Strand: Direct

Name: prfA

Synonym: Reut_A3044

Alternate gene names: 73542728

Gene position: 3348379-3349461 (Clockwise)

Preceding gene: 161611253

Following gene: 73542729

Centisome position: 87.96

GC content: 66.02

Gene sequence:

>1083_bases
ATGAAAGCCAGCATGCTTGCCAAGCTCGACCAACTGGCCGAGCGACTCGAGGAAGTCAACGCGCTGCTCGCACGCGAGGA
CGCGACCGCCAATATCGACCAGTACCGCAAGCTCAGCCGCGAGCATGCCGAACTGTCGCCGGTGGCCGAGCAGTACGGCT
TCTATCGACAGGCGCAGGACGACCTCGCTACGGCGCAGGCGCTGCTCGACGATCCGGAGATGAAGGACTTCGCCGCCGAC
GAGATCGCCAGCGCGCGTGAACGGCTGGAAAGCCTGGAAGGCAGCCTGCAGAAGCTGCTGCTGCCCAAGGACCCGAACGA
CGACCGCAACCTGATCCTCGAAATCCGCGCGGGCACCGGCGGCGAGGAAAGCGCGCTGTTCGCGGGCGACCTGCTGCGCA
TGTACACACGCTTTGCCGAGCGCCAGCGCTGGCAGGTGGAGATCATGAGCGAGTCGGAATCGGATCTTGGCGGTTACAAG
GAAGTGATCGTGCGCATTGCCGGCGATGCCGCGTTTTCGCGCCTGAAGTTCGAGTCGGGCGGCCACCGCGTGCAGCGCGT
GCCGGCCACCGAGGCGCAGGGCCGCATCCACACGTCGGCCTGCACCGTCGCGGTGATGCCCGAAGCCGATGAAGTCGGCG
ACGTCGAGATCAATCCGTCCGACCTGCGCATTGACACGTTCCGCGCATCGGGCGCCGGCGGTCAGCACGTCAACAAGACC
GACTCGGCCGTGCGCCTCACGCATCTGCCGACCGGGATCGTGGTCGAGTGCCAGGACGACCGCAGCCAGCACCGCAACAA
GGACAAGGCCATGAAGGTGCTGGCGGCGCGCATCAAGGACCAGCAGATGCGCGCCGCGCAGGCGAAGGAGGCAAGCACGC
GCCGCAACCTGATCGGCTCGGGCGACCGCAGCGACCGCATCCGCACGTACAACTTCCCGCAGGGACGCGTGACCGACCAC
CGCATCAACCTGACGCTCTACAAGATCGACATGATCATGGACGGCGACCTTGAAGAGCTGGTGTCCGCGCTGGCGGCCGA
GCATCAGGCCGACCAGTTGGCAGCGCTCGGAGAGGATAGCTAA

Upstream 100 bases:

>100_bases
CGCTAGCCGCGCTGATCGCTGCGCCCGCGCGCAATGCGCGCCGGCAACCGCCGCCGCCCCGATCGGGGCCCGGTATCCTG
CCTTCCCCACCCGGAATCCC

Downstream 100 bases:

>100_bases
GGCGCGCCTACGCGAGTGCCTTAAGCATTACATCGCAGCAACACCGCTCAACTGCATCGAACGCTTTGTATCTGTTGTAA
ATGCTTGAAACAGCACTGGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKASMLAKLDQLAERLEEVNALLAREDATANIDQYRKLSREHAELSPVAEQYGFYRQAQDDLATAQALLDDPEMKDFAAD
EIASARERLESLEGSLQKLLLPKDPNDDRNLILEIRAGTGGEESALFAGDLLRMYTRFAERQRWQVEIMSESESDLGGYK
EVIVRIAGDAAFSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADEVGDVEINPSDLRIDTFRASGAGGQHVNKT
DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDQQMRAAQAKEASTRRNLIGSGDRSDRIRTYNFPQGRVTDH
RINLTLYKIDMIMDGDLEELVSALAAEHQADQLAALGEDS

Sequences:

>Translated_360_residues
MKASMLAKLDQLAERLEEVNALLAREDATANIDQYRKLSREHAELSPVAEQYGFYRQAQDDLATAQALLDDPEMKDFAAD
EIASARERLESLEGSLQKLLLPKDPNDDRNLILEIRAGTGGEESALFAGDLLRMYTRFAERQRWQVEIMSESESDLGGYK
EVIVRIAGDAAFSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADEVGDVEINPSDLRIDTFRASGAGGQHVNKT
DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDQQMRAAQAKEASTRRNLIGSGDRSDRIRTYNFPQGRVTDH
RINLTLYKIDMIMDGDLEELVSALAAEHQADQLAALGEDS
>Mature_360_residues
MKASMLAKLDQLAERLEEVNALLAREDATANIDQYRKLSREHAELSPVAEQYGFYRQAQDDLATAQALLDDPEMKDFAAD
EIASARERLESLEGSLQKLLLPKDPNDDRNLILEIRAGTGGEESALFAGDLLRMYTRFAERQRWQVEIMSESESDLGGYK
EVIVRIAGDAAFSRLKFESGGHRVQRVPATEAQGRIHTSACTVAVMPEADEVGDVEINPSDLRIDTFRASGAGGQHVNKT
DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDQQMRAAQAKEASTRRNLIGSGDRSDRIRTYNFPQGRVTDH
RINLTLYKIDMIMDGDLEELVSALAAEHQADQLAALGEDS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=48.5915492957746, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI34577120, Length=328, Percent_Identity=40.2439024390244, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=47.8021978021978, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=65.9217877094972, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=38.9380530973451, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=343, Percent_Identity=33.5276967930029, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=40.5063291139241, Blast_Score=245, Evalue=8e-66,
Organism=Drosophila melanogaster, GI19921226, Length=264, Percent_Identity=44.6969696969697, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_CUPPJ (Q46WS9)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297248.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q46WS9
- SMR:   Q46WS9
- GeneID:   3609230
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3044
- NMPDR:   fig|264198.3.peg.2283
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   REUT264198:REUT_A3044-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 39991; Mature: 39991

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASMLAKLDQLAERLEEVNALLAREDATANIDQYRKLSREHAELSPVAEQYGFYRQAQD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAQALLDDPEMKDFAADEIASARERLESLEGSLQKLLLPKDPNDDRNLILEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GEESALFAGDLLRMYTRFAERQRWQVEIMSESESDLGGYKEVIVRIAGDAAFSRLKFESG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHEECCC
GHRVQRVPATEAQGRIHTSACTVAVMPEADEVGDVEINPSDLRIDTFRASGAGGQHVNKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDQQMRAAQAKEASTRRNLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFPQGRVTDHRINLTLYKIDMIMDGDLEELVSALAAEHQADQLAALGEDS
CCCCCCEEEECCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKASMLAKLDQLAERLEEVNALLAREDATANIDQYRKLSREHAELSPVAEQYGFYRQAQD
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLATAQALLDDPEMKDFAADEIASARERLESLEGSLQKLLLPKDPNDDRNLILEIRAGTG
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
GEESALFAGDLLRMYTRFAERQRWQVEIMSESESDLGGYKEVIVRIAGDAAFSRLKFESG
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHHHHHEECCC
GHRVQRVPATEAQGRIHTSACTVAVMPEADEVGDVEINPSDLRIDTFRASGAGGQHVNKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRLTHLPTGIVVECQDDRSQHRNKDKAMKVLAARIKDQQMRAAQAKEASTRRNLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSDRIRTYNFPQGRVTDHRINLTLYKIDMIMDGDLEELVSALAAEHQADQLAALGEDS
CCCCCCEEEECCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA