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The map label for this gene is paaI [H]

Identifier: 73542700

GI number: 73542700

Start: 3319276

End: 3319812

Strand: Direct

Name: paaI [H]

Synonym: Reut_A3016

Alternate gene names: 73542700

Gene position: 3319276-3319812 (Clockwise)

Preceding gene: 73542699

Following gene: 73542701

Centisome position: 87.2

GC content: 64.25

Gene sequence:

>537_bases
ATGCCTTCCTGGAAAAGCGCGCGCCGAAGTTCACCGGCCGCTGACCTGCAGGCGCCATCGTGCGCCGCCCCACCGAGACA
GGAGACCCGCTTGACCAAGAACCCGCAGGCACTTGCCGAGTCGGCCGCCGCCGCCATGTATGAAGCCGATACCTGTAGCC
GCTGGCTCGGCATCGTGGTGCAGGAAGTACGGCCCGGCTATGCCCGGCTGACCATGCCGGTACGCCCCGAATTCCTCAAT
GGCCACGGCATCTGCCACGGCGGCCTGATGTTTACACTGGCTGACTCCAGCTTCGCTTTCGCGTGCAATAGCCACAATAT
CAATACCGTGGCGGCAGGCTGCAGCATCGAGTTCCTCAAACCCGTGCATGGCGGCGACGTGCTGACCGCCGAAGCGGTGG
AACAGATCTTGTCGGGGCGGCACGGCATCTACGATATCCGCGTGACCAATCTTGCCGGCGAGGCCGTGGCGATGTTCCGC
GGCAAGTCGGCGCAGATCAAGGGACACGTGGTACCGCCGGACAGCAGCAGCACCTGA

Upstream 100 bases:

>100_bases
ATGTATGCGAGCGCAACCGCAACCCTCGATGCGCAGCTCGACCTAGAGCGCGACCTGCAGCGCGAGCTTGGGCAGTCCGC
CGACTATGCGGAAGGCGTCA

Downstream 100 bases:

>100_bases
AGTTCGCCAGGCCCGTGGCCTGACCCCGCACAAGACGATTTCACGATTCCATCGACAAGAACGCCCACGTGCAGGAGACG
ATATGAGCACGCGCCTGACC

Product: phenylacetic acid degradation-related protein:phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MPSWKSARRSSPAADLQAPSCAAPPRQETRLTKNPQALAESAAAAMYEADTCSRWLGIVVQEVRPGYARLTMPVRPEFLN
GHGICHGGLMFTLADSSFAFACNSHNINTVAAGCSIEFLKPVHGGDVLTAEAVEQILSGRHGIYDIRVTNLAGEAVAMFR
GKSAQIKGHVVPPDSSST

Sequences:

>Translated_178_residues
MPSWKSARRSSPAADLQAPSCAAPPRQETRLTKNPQALAESAAAAMYEADTCSRWLGIVVQEVRPGYARLTMPVRPEFLN
GHGICHGGLMFTLADSSFAFACNSHNINTVAAGCSIEFLKPVHGGDVLTAEAVEQILSGRHGIYDIRVTNLAGEAVAMFR
GKSAQIKGHVVPPDSSST
>Mature_177_residues
PSWKSARRSSPAADLQAPSCAAPPRQETRLTKNPQALAESAAAAMYEADTCSRWLGIVVQEVRPGYARLTMPVRPEFLNG
HGICHGGLMFTLADSSFAFACNSHNINTVAAGCSIEFLKPVHGGDVLTAEAVEQILSGRHGIYDIRVTNLAGEAVAMFRG
KSAQIKGHVVPPDSSST

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=128, Percent_Identity=46.875, Blast_Score=127, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 18920; Mature: 18788

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSWKSARRSSPAADLQAPSCAAPPRQETRLTKNPQALAESAAAAMYEADTCSRWLGIVV
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QEVRPGYARLTMPVRPEFLNGHGICHGGLMFTLADSSFAFACNSHNINTVAAGCSIEFLK
HHCCCCEEEEECCCCCCCCCCCCEEECCEEEEEECCCEEEEECCCCCEEEEECCEEEEEC
PVHGGDVLTAEAVEQILSGRHGIYDIRVTNLAGEAVAMFRGKSAQIKGHVVPPDSSST
CCCCCCEEEHHHHHHHHCCCCCEEEEEEECCCCHHHEEECCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
PSWKSARRSSPAADLQAPSCAAPPRQETRLTKNPQALAESAAAAMYEADTCSRWLGIVV
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QEVRPGYARLTMPVRPEFLNGHGICHGGLMFTLADSSFAFACNSHNINTVAAGCSIEFLK
HHCCCCEEEEECCCCCCCCCCCCEEECCEEEEEECCCEEEEECCCCCEEEEECCEEEEEC
PVHGGDVLTAEAVEQILSGRHGIYDIRVTNLAGEAVAMFRGKSAQIKGHVVPPDSSST
CCCCCCEEEHHHHHHHHCCCCCEEEEEEECCCCHHHEEECCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]