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The map label for this gene is hipO [H]

Identifier: 73542691

GI number: 73542691

Start: 3304266

End: 3305459

Strand: Direct

Name: hipO [H]

Synonym: Reut_A3007

Alternate gene names: 73542691

Gene position: 3304266-3305459 (Clockwise)

Preceding gene: 73542685

Following gene: 73542692

Centisome position: 86.81

GC content: 63.99

Gene sequence:

>1194_bases
ATGAAGCTCATTCCCGAAATCCTGCAGGCGCAAGCCGACATCCGCACGATCCGACGCGACATCCATGCGCATCCCGAGTT
GTGTTTCCAGGAACAACGCACCGCCGACGTGGTGGCGAGCAACCTGGAATCCTGGGGCATCGAAGTCCATCGCGGACTCG
GCAAGACAGGCCTGGTCGGCGTGATCCGCCAGGGCAACAGCGCACGCAGCATTGGCCTGCGCGCCGACATGGACGCGCTG
CCGCTGCAGGAAGCCAACACGTTCGGCCATCGCTCGCAGCATGATGGCCGCATGCACGCGTGCGGCCACGACGGCCACAC
TGCGATGCTGCTGGGCGCGGCGCGCTATCTGGCCGAGCATCGCAACTTCGACGGAACTATCAACCTGATCTTCCAGCCGG
CCGAAGAAGGCGGTGGCGGCGCGCGCGAGATGATCAAGGATGGCCTGTTCGAGCGCTTCCCGTGCGATGCGGTGTTCGGC
ATGCACAATTGGCCTGGCATGCCGGTCGGCGCATTCGGTACGCGCGCCGGCCCGCTGATGGCATCGAGCAATGAATTTCG
CATTGTCGTGCGCGGCAAGGGCGCGCACGCAGCCATGCCCAACAACGGCAGCGATCCGGTGTTCACCGCCGCACAGATCG
TGTCGGCATTGCAGGGCATCATCACGCGCAACAAGCGGCCGATCGACACGGCCGTGATCTCGGTCACGCAGTTCCATGCC
GGCGACGCGACCAATATCGTGCCGGACCAGGCGTGGATCGGCGGCACGGTGCGTACCTTTACGGTGCCCGTGCTCGACCT
GATCGAGCGGCGCATGGAGGAAGTCGCGCGTGCCGTGGCATCGGCCTTCGATTGCACGGTCGACTACGAGTTTCACCGCA
ACTATCCGCCGACAATCAATAGCGCCGCCGAGGCAGAGTTTGCCGCCGGCGTGGCGGCCGAACTGGTCGGTTTAGACAAC
GTCAATGCCGATGTCGAGCCGACGATGGGCGCCGAGGATTTCTCGTTCATGCTGCAGGAAAAGCCCGGCTGCTACCTGTT
CATCGGCAATGGCGACGGCGCGCATCGCGAATCCGGCCACGGCATGGGGCCGTGCATGCTGCACAACCCTAGCTACGACT
TCAACGACGAACTGCTGCCTGTCGGATCGACATTCTTTGTGAAGCTGGTGGAGAAGTGGCTGCCCCGGGCCTGA

Upstream 100 bases:

>100_bases
TACAATTTGGCGAAGGCTTGGAATCGGCCCGCTTTGGCGCTTTGCGGGCCGGCGTCGTATGATCGCGGTATCCATCGACA
GTCGTAGGGTCTCCGGCACC

Downstream 100 bases:

>100_bases
GCGAGCACGACAGCCATGGCTCGCATCGCATTGGACCTGGACATCGCAGATGGATTTGCGCGCGTAGCGCTTGAGAACAT
CGGGCGCCGCTACCCGTACA

Product: peptidase M20D, amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKLIPEILQAQADIRTIRRDIHAHPELCFQEQRTADVVASNLESWGIEVHRGLGKTGLVGVIRQGNSARSIGLRADMDAL
PLQEANTFGHRSQHDGRMHACGHDGHTAMLLGAARYLAEHRNFDGTINLIFQPAEEGGGGAREMIKDGLFERFPCDAVFG
MHNWPGMPVGAFGTRAGPLMASSNEFRIVVRGKGAHAAMPNNGSDPVFTAAQIVSALQGIITRNKRPIDTAVISVTQFHA
GDATNIVPDQAWIGGTVRTFTVPVLDLIERRMEEVARAVASAFDCTVDYEFHRNYPPTINSAAEAEFAAGVAAELVGLDN
VNADVEPTMGAEDFSFMLQEKPGCYLFIGNGDGAHRESGHGMGPCMLHNPSYDFNDELLPVGSTFFVKLVEKWLPRA

Sequences:

>Translated_397_residues
MKLIPEILQAQADIRTIRRDIHAHPELCFQEQRTADVVASNLESWGIEVHRGLGKTGLVGVIRQGNSARSIGLRADMDAL
PLQEANTFGHRSQHDGRMHACGHDGHTAMLLGAARYLAEHRNFDGTINLIFQPAEEGGGGAREMIKDGLFERFPCDAVFG
MHNWPGMPVGAFGTRAGPLMASSNEFRIVVRGKGAHAAMPNNGSDPVFTAAQIVSALQGIITRNKRPIDTAVISVTQFHA
GDATNIVPDQAWIGGTVRTFTVPVLDLIERRMEEVARAVASAFDCTVDYEFHRNYPPTINSAAEAEFAAGVAAELVGLDN
VNADVEPTMGAEDFSFMLQEKPGCYLFIGNGDGAHRESGHGMGPCMLHNPSYDFNDELLPVGSTFFVKLVEKWLPRA
>Mature_397_residues
MKLIPEILQAQADIRTIRRDIHAHPELCFQEQRTADVVASNLESWGIEVHRGLGKTGLVGVIRQGNSARSIGLRADMDAL
PLQEANTFGHRSQHDGRMHACGHDGHTAMLLGAARYLAEHRNFDGTINLIFQPAEEGGGGAREMIKDGLFERFPCDAVFG
MHNWPGMPVGAFGTRAGPLMASSNEFRIVVRGKGAHAAMPNNGSDPVFTAAQIVSALQGIITRNKRPIDTAVISVTQFHA
GDATNIVPDQAWIGGTVRTFTVPVLDLIERRMEEVARAVASAFDCTVDYEFHRNYPPTINSAAEAEFAAGVAAELVGLDN
VNADVEPTMGAEDFSFMLQEKPGCYLFIGNGDGAHRESGHGMGPCMLHNPSYDFNDELLPVGSTFFVKLVEKWLPRA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=415, Percent_Identity=27.2289156626506, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787598, Length=289, Percent_Identity=27.3356401384083, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 43113; Mature: 43113

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIPEILQAQADIRTIRRDIHAHPELCFQEQRTADVVASNLESWGIEVHRGLGKTGLVG
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEE
VIRQGNSARSIGLRADMDALPLQEANTFGHRSQHDGRMHACGHDGHTAMLLGAARYLAEH
EEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RNFDGTINLIFQPAEEGGGGAREMIKDGLFERFPCDAVFGMHNWPGMPVGAFGTRAGPLM
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCE
ASSNEFRIVVRGKGAHAAMPNNGSDPVFTAAQIVSALQGIITRNKRPIDTAVISVTQFHA
ECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC
GDATNIVPDQAWIGGTVRTFTVPVLDLIERRMEEVARAVASAFDCTVDYEFHRNYPPTIN
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCCCCCCCC
SAAEAEFAAGVAAELVGLDNVNADVEPTMGAEDFSFMLQEKPGCYLFIGNGDGAHRESGH
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
GMGPCMLHNPSYDFNDELLPVGSTFFVKLVEKWLPRA
CCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLIPEILQAQADIRTIRRDIHAHPELCFQEQRTADVVASNLESWGIEVHRGLGKTGLVG
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEE
VIRQGNSARSIGLRADMDALPLQEANTFGHRSQHDGRMHACGHDGHTAMLLGAARYLAEH
EEECCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
RNFDGTINLIFQPAEEGGGGAREMIKDGLFERFPCDAVFGMHNWPGMPVGAFGTRAGPLM
CCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCE
ASSNEFRIVVRGKGAHAAMPNNGSDPVFTAAQIVSALQGIITRNKRPIDTAVISVTQFHA
ECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECC
GDATNIVPDQAWIGGTVRTFTVPVLDLIERRMEEVARAVASAFDCTVDYEFHRNYPPTIN
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHCCCCCCCCCC
SAAEAEFAAGVAAELVGLDNVNADVEPTMGAEDFSFMLQEKPGCYLFIGNGDGAHRESGH
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCC
GMGPCMLHNPSYDFNDELLPVGSTFFVKLVEKWLPRA
CCCCEEEECCCCCCCCCEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]