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The map label for this gene is sir2 [H]

Identifier: 73542381

GI number: 73542381

Start: 2958065

End: 2959747

Strand: Reverse

Name: sir2 [H]

Synonym: Reut_A2696

Alternate gene names: 73542381

Gene position: 2959747-2958065 (Counterclockwise)

Preceding gene: 73542382

Following gene: 73542380

Centisome position: 77.75

GC content: 64.53

Gene sequence:

>1683_bases
ATGTATCAGTACGATCAATACGACCAGCGCATCGTCAACGAGCGCGTTGCCCAGTTCCGCGACCAGGTGCGCCGCCGCCT
GTCGGATGAGCTGACCGAGGAAGAATTCCTGCCGCTGCGCCTGCAGAACGGCCTGTACATGCAGCGCCATGCGTACATGC
TGCGCGTGGCGATCCCATATGGCCTGCTGGCTTCGAAGCAGCTGCGCAAGCTGGCCCATATCGCACGCGAGTACGACCGC
GGCTACGGCCATTTCTCGACGCGCCAGAACATCCAGTACAACTGGATGGAACTCGAGCGCGTGCCCGACGTGCTGGCCGA
GCTGGCCAGCGTCGAGATGCACGGCATCCAGACCTCGGGCAACTGTGTGCGCAACATCACCACCGATCACTTCGCCGGCG
TCGCGCCGGACGAGTCGGTGGACCCGCGCGTGCTGGCCGAACTGCTGCGCCAGTGGAGCACGTTCCAGCCCGAATTCGCC
TTCCTGCCGCGTAAGTTCAAGATCGCCATCTCGGCCTCGAAGGACGACCGCGCCGTGGTGCAGATGCATGACATCGGCAT
CTACGTGTACAAGAACGACGCCGGCGAGACCCGCATCCGCGTGCTGGCTGGCGGCGGCCTGGGCCGCACCCCGATCCTCG
GCACGATCATCAAGGACGATCTGCCGTGGCAGCACATGCTGACCTACGTCGAGTCCGCCATCCGCGTGTACAACCGCTAT
GGCCGCCGCGACAACAAGTACAAGGCGCGCATCAAGATCCTGGTGAAGGCCATCGGCGCCGAGAAGTTTGCCGAGGAAGT
CGAAGAGGAATGGCAGTACAGCAAGGACGGTCCCGGCACGCTGACGCAGGACGAGTTCGATCGCGTGGCGCAGTACTTCA
CGCCGCCGGCCTATGAAAAGCTGGCCGACACTGACGCCTCGTACGAAAAGCACCTGCTCGAGGACAAGGCCTTCGCGCGC
TGGGTCAGCCGCAGCGTGCACGGCCACAAGGTGCCGGGCTACGCCGCCGTGACGCTGTCGACCAAGCCGGGTCCGGTCTC
GCCTCCGGGTGACGCCACCGCCGAACAGATGGAAGCCGTGGCCGACCTGGCCGACCAATTCGGCTTCGGCGAGCTGCGCG
TGGCGCACGAGCAGAACCTGGTGCTGCCGGACGTGAAGAAGCACGACCTGTACGACCTGTGGCAACTCGCGAAGAAGGCC
GGCCTGGCTACGGCCAACATCGGCCTGCTGACCGACATCATCGCCTGCCCGGGCGGCGACTTCTGCTCGCTGGCCAATGC
CAAGTCGATTCCGATCGCGCTGGCCATTCAGGAGCGCTTCGACAACCTGGACTTCGTCCATGACCTGGGCGAAGTGTCGC
TGAACATCTCGGGCTGCATCAACGCCTGCGGCCATCACCACGTCGGCAACATCGGCATCCTCGGCGTCGACAAGGACGGT
TCGGAGTGGTACCAGGTCACGCTCGGCGGCGCGCAAGGCAACAGCACGGCGCTGGGCAAGGTCATCGGCCCGTCGTTCAG
CGCAGAGGAAATGCCGGACGTGATCTCGCGCATTATCGAGACCTTCGTGGCGAACCGCATCGAAGACGAACGCTTCATCG
AGACCGTGAACCGGATCGGCATCGCCCCGTTCAAGGAGCGCGTGTACGCCGACAAGCAACCGCGCGAGCGCGCAGAAGCC
TGA

Upstream 100 bases:

>100_bases
TGCCCGAGCGGCTCGTACGCGCGGCCCTGGCCACCACGCTGACGCTGGTCGCCATCAAGCTCGTCTCCTGAATTCAACGA
ATACTGAACGGACCCACACC

Downstream 100 bases:

>100_bases
GGACCGGAGAAACAGACATGGCAAAGATTATTCAACTGCAACGTACGGCCGACGCCGTCACCCCGGTCATCGTCGAAGAC
AGCTGGACCGTGCTGCGCGG

Product: sulfite reductase (NADPH) beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MYQYDQYDQRIVNERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQLRKLAHIAREYDR
GYGHFSTRQNIQYNWMELERVPDVLAELASVEMHGIQTSGNCVRNITTDHFAGVAPDESVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISASKDDRAVVQMHDIGIYVYKNDAGETRIRVLAGGGLGRTPILGTIIKDDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAEEVEEEWQYSKDGPGTLTQDEFDRVAQYFTPPAYEKLADTDASYEKHLLEDKAFAR
WVSRSVHGHKVPGYAAVTLSTKPGPVSPPGDATAEQMEAVADLADQFGFGELRVAHEQNLVLPDVKKHDLYDLWQLAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDFVHDLGEVSLNISGCINACGHHHVGNIGILGVDKDG
SEWYQVTLGGAQGNSTALGKVIGPSFSAEEMPDVISRIIETFVANRIEDERFIETVNRIGIAPFKERVYADKQPRERAEA

Sequences:

>Translated_560_residues
MYQYDQYDQRIVNERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQLRKLAHIAREYDR
GYGHFSTRQNIQYNWMELERVPDVLAELASVEMHGIQTSGNCVRNITTDHFAGVAPDESVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISASKDDRAVVQMHDIGIYVYKNDAGETRIRVLAGGGLGRTPILGTIIKDDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAEEVEEEWQYSKDGPGTLTQDEFDRVAQYFTPPAYEKLADTDASYEKHLLEDKAFAR
WVSRSVHGHKVPGYAAVTLSTKPGPVSPPGDATAEQMEAVADLADQFGFGELRVAHEQNLVLPDVKKHDLYDLWQLAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDFVHDLGEVSLNISGCINACGHHHVGNIGILGVDKDG
SEWYQVTLGGAQGNSTALGKVIGPSFSAEEMPDVISRIIETFVANRIEDERFIETVNRIGIAPFKERVYADKQPRERAEA
>Mature_560_residues
MYQYDQYDQRIVNERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPYGLLASKQLRKLAHIAREYDR
GYGHFSTRQNIQYNWMELERVPDVLAELASVEMHGIQTSGNCVRNITTDHFAGVAPDESVDPRVLAELLRQWSTFQPEFA
FLPRKFKIAISASKDDRAVVQMHDIGIYVYKNDAGETRIRVLAGGGLGRTPILGTIIKDDLPWQHMLTYVESAIRVYNRY
GRRDNKYKARIKILVKAIGAEKFAEEVEEEWQYSKDGPGTLTQDEFDRVAQYFTPPAYEKLADTDASYEKHLLEDKAFAR
WVSRSVHGHKVPGYAAVTLSTKPGPVSPPGDATAEQMEAVADLADQFGFGELRVAHEQNLVLPDVKKHDLYDLWQLAKKA
GLATANIGLLTDIIACPGGDFCSLANAKSIPIALAIQERFDNLDFVHDLGEVSLNISGCINACGHHHVGNIGILGVDKDG
SEWYQVTLGGAQGNSTALGKVIGPSFSAEEMPDVISRIIETFVANRIEDERFIETVNRIGIAPFKERVYADKQPRERAEA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 63044; Mature: 63044

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQYDQYDQRIVNERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCEEEHHHHEEEEEECCH
GLLASKQLRKLAHIAREYDRGYGHFSTRQNIQYNWMELERVPDVLAELASVEMHGIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCC
NCVRNITTDHFAGVAPDESVDPRVLAELLRQWSTFQPEFAFLPRKFKIAISASKDDRAVV
CHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCCCEEE
QMHDIGIYVYKNDAGETRIRVLAGGGLGRTPILGTIIKDDLPWQHMLTYVESAIRVYNRY
EEEEEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKAIGAEKFAEEVEEEWQYSKDGPGTLTQDEFDRVAQYFTPPAYEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
LADTDASYEKHLLEDKAFARWVSRSVHGHKVPGYAAVTLSTKPGPVSPPGDATAEQMEAV
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
ADLADQFGFGELRVAHEQNLVLPDVKKHDLYDLWQLAKKAGLATANIGLLTDIIACPGGD
HHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
FCSLANAKSIPIALAIQERFDNLDFVHDLGEVSLNISGCINACGHHHVGNIGILGVDKDG
CCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHEEEHHHHHHHCCCCCCCCEEEEEECCCC
SEWYQVTLGGAQGNSTALGKVIGPSFSAEEMPDVISRIIETFVANRIEDERFIETVNRIG
CCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
IAPFKERVYADKQPRERAEA
CCHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MYQYDQYDQRIVNERVAQFRDQVRRRLSDELTEEEFLPLRLQNGLYMQRHAYMLRVAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEECCCEEEHHHHEEEEEECCH
GLLASKQLRKLAHIAREYDRGYGHFSTRQNIQYNWMELERVPDVLAELASVEMHGIQTSG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCC
NCVRNITTDHFAGVAPDESVDPRVLAELLRQWSTFQPEFAFLPRKFKIAISASKDDRAVV
CHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCEEEEEEECCCCCCEEE
QMHDIGIYVYKNDAGETRIRVLAGGGLGRTPILGTIIKDDLPWQHMLTYVESAIRVYNRY
EEEEEEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNKYKARIKILVKAIGAEKFAEEVEEEWQYSKDGPGTLTQDEFDRVAQYFTPPAYEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHH
LADTDASYEKHLLEDKAFARWVSRSVHGHKVPGYAAVTLSTKPGPVSPPGDATAEQMEAV
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHH
ADLADQFGFGELRVAHEQNLVLPDVKKHDLYDLWQLAKKAGLATANIGLLTDIIACPGGD
HHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCC
FCSLANAKSIPIALAIQERFDNLDFVHDLGEVSLNISGCINACGHHHVGNIGILGVDKDG
CCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHHEEEHHHHHHHCCCCCCCCEEEEEECCCC
SEWYQVTLGGAQGNSTALGKVIGPSFSAEEMPDVISRIIETFVANRIEDERFIETVNRIG
CCEEEEEEECCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
IAPFKERVYADKQPRERAEA
CCHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA