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The map label for this gene is lolA

Identifier: 73542284

GI number: 73542284

Start: 2850584

End: 2851222

Strand: Reverse

Name: lolA

Synonym: Reut_A2599

Alternate gene names: 73542284

Gene position: 2851222-2850584 (Counterclockwise)

Preceding gene: 73542285

Following gene: 73542283

Centisome position: 74.9

GC content: 62.28

Gene sequence:

>639_bases
ATGCGAAAACGCATTCTCGTGTCGGCCTGTGCAGCGCTCGCCGTATTTGCTGCGCACATGCCCACGGCGCTCGCCGCCGC
CACTGACCAGTTGCAAAGCTTCGTGACCGGCGTGAAGAGCGCCAAGGGCGAATTCACGCAGCGCCAGGTCAAGGGGCAGG
GCGACAGCCTCAAGGTCACCGGCACGTCGAGCGGCAGCTTTGTGTTCTCGCGCCCGGGCAAGTTCACGTGGCGCTATGCC
AAGCCCTATGAGCAGTTGTTGCAGGCAGACGGGCAGACGCTCTACATCTACGACAAGGACCTGAACCAGGTCACCGAGCG
CAAGCTCGACGGCGCATTGGGCTCGAGTCCGGCAGCCATCCTGTTCGGCAGCAATGACCTCGAGAAGAATTTCACGGTCA
AGAACGGCCCGACGCGTGACGGCGTCGAGTGGCTGGAGCTGACGCCGAAGTCGAAGGACACGCAGTTCGAGCGGATCGGC
ATTGGCTTCAAGGGCGGCAATCTGGAGGCGATGGAGTTGCGCGACGCGTTCGGCAACACCACGCTGCTGACGTTCTCGGC
CATGCAGAAGAATCCGCAACTGCCCGCGAATGCATTCCGGTTCACGGTGCCCAAGGGCGCCGATGTGATGAAGCAATGA

Upstream 100 bases:

>100_bases
CGGCGCCGGCCGCACGCTCTAACCTGACAGGTTGCGCCGCCGCCTGATCCAATTGGCACGCGGCGGCGCGGCGCCTTTTC
CATCAGCAAGGATCTGATAC

Downstream 100 bases:

>100_bases
GAGGCGCCATGTCCGCACTGAAACCCGTGGTGTCCAGCGACGCGCGCCGTGTCGCGGTGGCTGCCGTATGCATGGCGGTC
GCGCTTGGCTTGTCCGCGTG

Product: outer-membrane lipoprotein carrier protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRKRILVSACAALAVFAAHMPTALAAATDQLQSFVTGVKSAKGEFTQRQVKGQGDSLKVTGTSSGSFVFSRPGKFTWRYA
KPYEQLLQADGQTLYIYDKDLNQVTERKLDGALGSSPAAILFGSNDLEKNFTVKNGPTRDGVEWLELTPKSKDTQFERIG
IGFKGGNLEAMELRDAFGNTTLLTFSAMQKNPQLPANAFRFTVPKGADVMKQ

Sequences:

>Translated_212_residues
MRKRILVSACAALAVFAAHMPTALAAATDQLQSFVTGVKSAKGEFTQRQVKGQGDSLKVTGTSSGSFVFSRPGKFTWRYA
KPYEQLLQADGQTLYIYDKDLNQVTERKLDGALGSSPAAILFGSNDLEKNFTVKNGPTRDGVEWLELTPKSKDTQFERIG
IGFKGGNLEAMELRDAFGNTTLLTFSAMQKNPQLPANAFRFTVPKGADVMKQ
>Mature_212_residues
MRKRILVSACAALAVFAAHMPTALAAATDQLQSFVTGVKSAKGEFTQRQVKGQGDSLKVTGTSSGSFVFSRPGKFTWRYA
KPYEQLLQADGQTLYIYDKDLNQVTERKLDGALGSSPAAILFGSNDLEKNFTVKNGPTRDGVEWLELTPKSKDTQFERIG
IGFKGGNLEAMELRDAFGNTTLLTFSAMQKNPQLPANAFRFTVPKGADVMKQ

Specific function: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the l

COG id: COG2834

COG function: function code M; Outer membrane lipoprotein-sorting protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolA family

Homologues:

Organism=Escherichia coli, GI145693108, Length=216, Percent_Identity=28.2407407407407, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): LOLA_CUPPJ (Q46Y23)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296804.1
- ProteinModelPortal:   Q46Y23
- SMR:   Q46Y23
- GeneID:   3610166
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2599
- NMPDR:   fig|264198.3.peg.3353
- HOGENOM:   HBG688940
- OMA:   MELRDAF
- ProtClustDB:   PRK00031
- BioCyc:   REUT264198:REUT_A2599-MONOMER
- HAMAP:   MF_00240
- InterPro:   IPR004564
- InterPro:   IPR018323
- TIGRFAMs:   TIGR00547

Pfam domain/function: PF03548 LolA

EC number: NA

Molecular weight: Translated: 23139; Mature: 23139

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRILVSACAALAVFAAHMPTALAAATDQLQSFVTGVKSAKGEFTQRQVKGQGDSLKVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEE
GTSSGSFVFSRPGKFTWRYAKPYEQLLQADGQTLYIYDKDLNQVTERKLDGALGSSPAAI
ECCCCCEEEECCCCEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEE
LFGSNDLEKNFTVKNGPTRDGVEWLELTPKSKDTQFERIGIGFKGGNLEAMELRDAFGNT
EECCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEHEEHHHHCCCE
TLLTFSAMQKNPQLPANAFRFTVPKGADVMKQ
EEEEEEEHHCCCCCCCCCEEEECCCCCHHHCC
>Mature Secondary Structure
MRKRILVSACAALAVFAAHMPTALAAATDQLQSFVTGVKSAKGEFTQRQVKGQGDSLKVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEE
GTSSGSFVFSRPGKFTWRYAKPYEQLLQADGQTLYIYDKDLNQVTERKLDGALGSSPAAI
ECCCCCEEEECCCCEEEEECCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCEEE
LFGSNDLEKNFTVKNGPTRDGVEWLELTPKSKDTQFERIGIGFKGGNLEAMELRDAFGNT
EECCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEHEEHHHHCCCE
TLLTFSAMQKNPQLPANAFRFTVPKGADVMKQ
EEEEEEEHHCCCCCCCCCEEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA