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The map label for this gene is pheA [H]

Identifier: 73542260

GI number: 73542260

Start: 2820025

End: 2821173

Strand: Reverse

Name: pheA [H]

Synonym: Reut_A2575

Alternate gene names: 73542260

Gene position: 2821173-2820025 (Counterclockwise)

Preceding gene: 73542261

Following gene: 73542259

Centisome position: 74.11

GC content: 65.88

Gene sequence:

>1149_bases
ATGACAAGCGAAGACAACAACACCGCGCGCGAGGAGACGCAGCAGAGCGGTGCTTCCCCGAGCGCGGCGGAACGTGCCCT
CGGTGCCGAACTGGCCCCGTTGCGCGAGCAGATCGATACCGTCGACCGTGAACTGCTGGCCATGCTGAACCGGCGCGCGC
AGCTTGCGCTCGATGTCGGCGAGGTAAAGAAGAAGTACGGCGCGCCGGTGTTCCGCCCCGAGCGCGAACTGCAGGTCATT
CGCAAGGTGCAGGCCGCCAATCCGGGTCCGCTGCTCGGCGAGAGCGTGGCGTCGATCTGGCGCGAGGTCATGTCGGCATG
CCGTGGCCTGGAAAAGCCGCTTGAGATCGCCTTCCTCGGCCCGGCCGGTACGTTCTCGGAACAGGCTTTGTACGCGCACT
TCGGCCATGAAGTGAGCGGCGTGCCGTGCCCGAGCATCGACGAAGTATTCCGCGCCGTTGAGGCGGGGACCGTCGAGTAC
GGCGTGGTTCCGGTGGAAAACTCCACCGAGGGCGCCGTGTCGCGCACGCTGGACCTGTTTCTGCAGACCTCGCTGAAGAT
CAGCGGCGAAATCGCGCTGAAGGTGCACCACAACCTGATGGCCGCTTCGCCGGACATGAACGGCGTGAAGGTCGTGCGCG
CGCATGCGCAGGCGCTCGCGCAGTGCCAGCACTGGCTGACGGCGAACTATCCGCACCTCGAGCGGCAGGCCGTATCGAGC
AATGCCGAGGCGGCGCGCATGGCGAGCGAAGATCCCACGGTCGCGGCCATTGCCGGCGAATCGGCTGCTAATCGCTATCA
CCTCCATATCATCCGGCCGCATATCCAGGACGATCCGCACAACCGCACGCGCTTTGCCGTGATTGGCCGGTACGAGACCG
AGCCGTCGGGCAGCGACCAGACTTCGATGATCCTGTCGGTGCCCAACAAGGCCGGCGCGGTCTACCAGTTGCTCGCGCCG
CTGGCGGAGAACGGTGTGTCGATGTGCCGCTTCGAATCGCGCCCCGCGCGCAGTGGTGCATGGGAGTACTACTTCTACGT
CGATGTGGAAGGCCACCAGCACGAGCCGGCCGTGGCGCGCGCGATCGAGACGCTGCGGCGCAACGCAGCGTACCTGAAGG
TGCTCGGGTCGTACCCGTCGAGCAAGTAA

Upstream 100 bases:

>100_bases
CTGCCTGACGGCGCTGCGGCGGACAGCCGCGCGCGAACGGCGCGCGGCATTGTTTTATTCTCAGGCCCTCCCTGCGGAGC
GGCCCAACCGGTTCCTGCAA

Downstream 100 bases:

>100_bases
GCACGCGCAGATACGAGGCAAGGAGACAAGATGGCAGAGCAAGGCAAGCAAGGCGGCAAGGTACCGTTCGGCCCGGATTA
CGTCAGGGCGATTTCGCCTT

Product: prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTSEDNNTAREETQQSGASPSAAERALGAELAPLREQIDTVDRELLAMLNRRAQLALDVGEVKKKYGAPVFRPERELQVI
RKVQAANPGPLLGESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEY
GVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMAASPDMNGVKVVRAHAQALAQCQHWLTANYPHLERQAVSS
NAEAARMASEDPTVAAIAGESAANRYHLHIIRPHIQDDPHNRTRFAVIGRYETEPSGSDQTSMILSVPNKAGAVYQLLAP
LAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVARAIETLRRNAAYLKVLGSYPSSK

Sequences:

>Translated_382_residues
MTSEDNNTAREETQQSGASPSAAERALGAELAPLREQIDTVDRELLAMLNRRAQLALDVGEVKKKYGAPVFRPERELQVI
RKVQAANPGPLLGESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEY
GVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMAASPDMNGVKVVRAHAQALAQCQHWLTANYPHLERQAVSS
NAEAARMASEDPTVAAIAGESAANRYHLHIIRPHIQDDPHNRTRFAVIGRYETEPSGSDQTSMILSVPNKAGAVYQLLAP
LAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVARAIETLRRNAAYLKVLGSYPSSK
>Mature_381_residues
TSEDNNTAREETQQSGASPSAAERALGAELAPLREQIDTVDRELLAMLNRRAQLALDVGEVKKKYGAPVFRPERELQVIR
KVQAANPGPLLGESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEYG
VVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMAASPDMNGVKVVRAHAQALAQCQHWLTANYPHLERQAVSSN
AEAARMASEDPTVAAIAGESAANRYHLHIIRPHIQDDPHNRTRFAVIGRYETEPSGSDQTSMILSVPNKAGAVYQLLAPL
AENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVARAIETLRRNAAYLKVLGSYPSSK

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=374, Percent_Identity=30.4812834224599, Blast_Score=169, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=24.7678018575851, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010957
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01842 ACT; PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 41672; Mature: 41541

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEDNNTAREETQQSGASPSAAERALGAELAPLREQIDTVDRELLAMLNRRAQLALDVG
CCCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECHH
EVKKKYGAPVFRPERELQVIRKVQAANPGPLLGESVASIWREVMSACRGLEKPLEIAFLG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PAGTFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSRTLDLF
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
LQTSLKISGEIALKVHHNLMAASPDMNGVKVVRAHAQALAQCQHWLTANYPHLERQAVSS
HHHCEEECCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
NAEAARMASEDPTVAAIAGESAANRYHLHIIRPHIQDDPHNRTRFAVIGRYETEPSGSDQ
CCHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCC
TSMILSVPNKAGAVYQLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVAR
CEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHH
AIETLRRNAAYLKVLGSYPSSK
HHHHHHHCHHHEEEECCCCCCC
>Mature Secondary Structure 
TSEDNNTAREETQQSGASPSAAERALGAELAPLREQIDTVDRELLAMLNRRAQLALDVG
CCCCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHEEECHH
EVKKKYGAPVFRPERELQVIRKVQAANPGPLLGESVASIWREVMSACRGLEKPLEIAFLG
HHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PAGTFSEQALYAHFGHEVSGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSRTLDLF
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHH
LQTSLKISGEIALKVHHNLMAASPDMNGVKVVRAHAQALAQCQHWLTANYPHLERQAVSS
HHHCEEECCEEEEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
NAEAARMASEDPTVAAIAGESAANRYHLHIIRPHIQDDPHNRTRFAVIGRYETEPSGSDQ
CCHHHHHCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCC
TSMILSVPNKAGAVYQLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVAR
CEEEEECCCCCHHHHHHHHHHHHCCHHEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHH
AIETLRRNAAYLKVLGSYPSSK
HHHHHHHCHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10094619 [H]