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The map label for this gene is trmD [H]

Identifier: 73542223

GI number: 73542223

Start: 2782912

End: 2783694

Strand: Reverse

Name: trmD [H]

Synonym: Reut_A2538

Alternate gene names: 73542223

Gene position: 2783694-2782912 (Counterclockwise)

Preceding gene: 73542224

Following gene: 73542222

Centisome position: 73.13

GC content: 61.43

Gene sequence:

>783_bases
ATGCAGTTCGATGTAATCACGCTGTTTCCCGAAATGTTTCGCGCGCTGACCGACTGGGGTATCACCAGCCGGGCAGCCAA
GCAGCAGCGGTATGCCCTGCGCACGTGGAATCCACGCGATTTCACCACGGACAACTACCGCACGATCGATGATCGTCCGT
ATGGTGGTGGTCCCGGCATGGTCATGCTGCCCAGGCCACTGGACGATGCGATCGATGCCGCTTCGGCGGCGCAGTCGCAG
GCCGGCGTAGATACGCCGCATGTGGTGCTGATGTCGCCACAGGGCGCGACGCTGACGCACGAGAAGGTCATGTCGCTCGC
GCAGCGGCCGGGTCTGGTGCTGTTATGTGGCCGCTATGAGGCCATCGACCAGCGCCTGATCGACCGTCGCGTGCATGAGG
AAATCAGCCTTGGCGATTTCGTGCTGTCCGGCGGCGAATTGCCGGCCATGGCGCTGATCGATGCAGTAGTGCGCCACTTG
CCCGGTGTGCTGGGCGATGCGCAGTCGGCAGTGCAAGACAGTTTCGTCAACGGGTTGCTTGATTGTCCGCATTACACGCG
GCCCGAAGAGTACGAGGGTGTGCGGGTACCCGAGATCCTGCTCGGTGGCCATCATGCCGAGATTGAAAAGTGGCGGCGCC
AGCAGGCGTTGGCCAATACTGCGAGAAAGCGCCCCGATCTGATCGAAGCGGCTCGCAAGCAAGGTTTGCTGACACGTGCC
GACGAGAAATTCTTGTCGGAATGGTTGGTGAAAGAAGGGCGGGAGATGTCTCCCGCCAGGTAA

Upstream 100 bases:

>100_bases
CGTTCCTGCGCACGGTCGACACCGCGGGCAAGCGCATCGTGGTGGACTGGGGGCTCGACTACTGAATTGCCGAAGCCGGC
AGTTCGGACGGGGAGGGCGG

Downstream 100 bases:

>100_bases
AACCCCATCCTCTGCCGGGGCCCGGGCAATGCCTGGGGCATACAACGCTGGCACGATGGTTACGGAGAAAAAACGATGAA
CATCATCGAGCAGATCGAGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLPRPLDDAIDAASAAQSQ
AGVDTPHVVLMSPQGATLTHEKVMSLAQRPGLVLLCGRYEAIDQRLIDRRVHEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPEILLGGHHAEIEKWRRQQALANTARKRPDLIEAARKQGLLTRA
DEKFLSEWLVKEGREMSPAR

Sequences:

>Translated_260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLPRPLDDAIDAASAAQSQ
AGVDTPHVVLMSPQGATLTHEKVMSLAQRPGLVLLCGRYEAIDQRLIDRRVHEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPEILLGGHHAEIEKWRRQQALANTARKRPDLIEAARKQGLLTRA
DEKFLSEWLVKEGREMSPAR
>Mature_260_residues
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTTDNYRTIDDRPYGGGPGMVMLPRPLDDAIDAASAAQSQ
AGVDTPHVVLMSPQGATLTHEKVMSLAQRPGLVLLCGRYEAIDQRLIDRRVHEEISLGDFVLSGGELPAMALIDAVVRHL
PGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPEILLGGHHAEIEKWRRQQALANTARKRPDLIEAARKQGLLTRA
DEKFLSEWLVKEGREMSPAR

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=255, Percent_Identity=52.156862745098, Blast_Score=259, Evalue=9e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29051; Mature: 29051

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCE
VMLPRPLDDAIDAASAAQSQAGVDTPHVVLMSPQGATLTHEKVMSLAQRPGLVLLCGRYE
EEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCHH
AIDQRLIDRRVHEEISLGDFVLSGGELPAMALIDAVVRHLPGVLGDAQSAVQDSFVNGLL
HHHHHHHHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
DCPHYTRPEEYEGVRVPEILLGGHHAEIEKWRRQQALANTARKRPDLIEAARKQGLLTRA
CCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
DEKFLSEWLVKEGREMSPAR
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTTDNYRTIDDRPYGGGPGM
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCE
VMLPRPLDDAIDAASAAQSQAGVDTPHVVLMSPQGATLTHEKVMSLAQRPGLVLLCGRYE
EEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCHH
AIDQRLIDRRVHEEISLGDFVLSGGELPAMALIDAVVRHLPGVLGDAQSAVQDSFVNGLL
HHHHHHHHHHHHHHHCHHHHEECCCCCHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHH
DCPHYTRPEEYEGVRVPEILLGGHHAEIEKWRRQQALANTARKRPDLIEAARKQGLLTRA
CCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHH
DEKFLSEWLVKEGREMSPAR
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA