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The map label for this gene is glcA [H]

Identifier: 73542039

GI number: 73542039

Start: 2592772

End: 2594448

Strand: Direct

Name: glcA [H]

Synonym: Reut_A2353

Alternate gene names: 73542039

Gene position: 2592772-2594448 (Clockwise)

Preceding gene: 73542037

Following gene: 73542045

Centisome position: 68.11

GC content: 63.98

Gene sequence:

>1677_bases
GTGTGGAGTCAAGTCTATGACCCGCTAGGCAATATGGCGCTGTCGACCATTGCTGCGGGTGTCCCGGTGGCCGTGCTGCT
TGCAGCGCTGGCTTTCTTTCACATGCAGGCCCACCTGGCCGCCGGTCTGGCGCTGGTCGTGGGCGTCGTGGTTGCTGCAG
CAGTCTTCGGCATGCCGGCAGCCATGGCTGGCAAGGCCGCGGGCCTCGGCATCGTATCGGGTCTCTTCCCGATTGGGTGG
ATTGTCCTCAACATCATCTTTCTGCATCGGCTGACGACGCTGAACGGGTCATTCAAGGTCTTGCAGAACTCGATCTCCGG
CGTGACCGAAGACCGTCGCCTGCAGCTGCTGCTGGTTGCCTTCAGCTTCGGCGCGTTTTTTGAAGGCGCGGCCGGCTTCG
GCACGCCGGTGGCCGTGACCGGCGCCATCCTGATCGGCCTGGGCTTCTCGCCGCTGGCCGCCTCGGGCCTGGCGCTGATC
GCCAACACCGCGCCGGTGGCCTTCGGCGCGCTCGGGGCCCCGATCATCGGGCTGTCGTCGGTGACGGGCATCGACCAGGT
CACGCTGTCGGCCATGATCGGCCGCCAGTTGCCCTTCTTCTCGGTGCTGGTGCCGTTCTGGCTGATCTGGGCCTTTGCTG
GCTTCCGCGGCATGCTCGCCATCTGGCCGGCGATCCTGGTCGCGGGCGTGACCTTCGCGGTGCCGCAGTTCCTGGTCTCG
AATTTCCATGGCCCGTGGCTGGTGGACGTGATCTCGTCGCTAATCTCGATGGGCTGCCTGACGCTGTTCCTGAAGATCTG
GCAACCGAAGGAAGTCTGGACGTCGACCAAGATCCTCGGCCGCCACGACGACTCCAAGGTCGACCACCCCGAGGCACTGG
CCGCCGACGCCGCCGCCAGCGCCGCCTCGGCCGGCATCTCGGTCGCCAAGGCCTGGCTGCCGTGGATCATCCTGACCATC
TTCGTGTTCGTCTGGGGCATCCCCGAGTTCAAGAAGTTCATGGATAACTTGTGGGCGTTCAAGTTCCCGATCCCGGGCCT
GGACAAGGCAATCATCAAGCATCCGCCGGTCACGCCGAAAGAAATCGCCGAACCGGCGGTGTTCCTCTTCAACGTGCTGT
CGATGGCCGGCACCGGCATCCTGGTCTCCGCCATTCTCGGCGGCCTGCTGATGGGCTACTCGGTGCCGCGTCTCGTCAAG
GAATACTGGGAAACCATCAAGCTGACCAAGTTCTCGCTGCTGACCATCAGTGCGATGTTCGGCATCGGCTACCTGACCCG
CTACTCCGGGCTGGATGCCACGCTGGGTCTCGCCTTCGCACAGACCGGCGTGCTCTACCCACTGTTCGGCACCATGCTGG
GCTGGCTGGGCGTGGCGCTGACGGGCTCGGACACCGCGTCCAACGTGCTGTTCGGCGGCCTGCAGAAGACCACGGCCGAG
CAGCTTGGCCTGTCGCCGGTGTTGATGGCCGCGGCCAACAGTTCAGGCGGCGTGATGGGCAAAATGATCGACGCGCAGTC
CATCGTGGTGGCCTCGACGGCTACCAAGTGGTATGGCCATGAAGGCGACATCCTGCGTTACGTGTTCTTCCATTCGATCG
CGCTGGCGATCCTGGTTGGGATCTTCGTCACGCTGCAGGCGTATGTGGAGCCGTTCACGCATATGGTCATTCATTAA

Upstream 100 bases:

>100_bases
TGCGGTTGACGCCGCACGTCTGTACGCGTTGTACACCGGCAGCCCAGGGCACGTAGATCGCCCGGGACGCCCGAGCCAAA
CCTGAGGAGGATTATCAATC

Downstream 100 bases:

>100_bases
CGAGCCACAACGAGCCGTCCCGCACACCAGGCGGGCGTTTCGCCTGCGCCATGCAGCCCCGCTCCCAGCGGGGCTGTTTT
TTTGTCCGGTCAGAGCACGC

Product: L-lactate permease

Products: lactate [Cytoplasm]; Proton [Cytoplasm]; glycolate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MWSQVYDPLGNMALSTIAAGVPVAVLLAALAFFHMQAHLAAGLALVVGVVVAAAVFGMPAAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQNSISGVTEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAFGALGAPIIGLSSVTGIDQVTLSAMIGRQLPFFSVLVPFWLIWAFAGFRGMLAIWPAILVAGVTFAVPQFLVS
NFHGPWLVDVISSLISMGCLTLFLKIWQPKEVWTSTKILGRHDDSKVDHPEALAADAAASAASAGISVAKAWLPWIILTI
FVFVWGIPEFKKFMDNLWAFKFPIPGLDKAIIKHPPVTPKEIAEPAVFLFNVLSMAGTGILVSAILGGLLMGYSVPRLVK
EYWETIKLTKFSLLTISAMFGIGYLTRYSGLDATLGLAFAQTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSIALAILVGIFVTLQAYVEPFTHMVIH

Sequences:

>Translated_558_residues
MWSQVYDPLGNMALSTIAAGVPVAVLLAALAFFHMQAHLAAGLALVVGVVVAAAVFGMPAAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQNSISGVTEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAFGALGAPIIGLSSVTGIDQVTLSAMIGRQLPFFSVLVPFWLIWAFAGFRGMLAIWPAILVAGVTFAVPQFLVS
NFHGPWLVDVISSLISMGCLTLFLKIWQPKEVWTSTKILGRHDDSKVDHPEALAADAAASAASAGISVAKAWLPWIILTI
FVFVWGIPEFKKFMDNLWAFKFPIPGLDKAIIKHPPVTPKEIAEPAVFLFNVLSMAGTGILVSAILGGLLMGYSVPRLVK
EYWETIKLTKFSLLTISAMFGIGYLTRYSGLDATLGLAFAQTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSIALAILVGIFVTLQAYVEPFTHMVIH
>Mature_558_residues
MWSQVYDPLGNMALSTIAAGVPVAVLLAALAFFHMQAHLAAGLALVVGVVVAAAVFGMPAAMAGKAAGLGIVSGLFPIGW
IVLNIIFLHRLTTLNGSFKVLQNSISGVTEDRRLQLLLVAFSFGAFFEGAAGFGTPVAVTGAILIGLGFSPLAASGLALI
ANTAPVAFGALGAPIIGLSSVTGIDQVTLSAMIGRQLPFFSVLVPFWLIWAFAGFRGMLAIWPAILVAGVTFAVPQFLVS
NFHGPWLVDVISSLISMGCLTLFLKIWQPKEVWTSTKILGRHDDSKVDHPEALAADAAASAASAGISVAKAWLPWIILTI
FVFVWGIPEFKKFMDNLWAFKFPIPGLDKAIIKHPPVTPKEIAEPAVFLFNVLSMAGTGILVSAILGGLLMGYSVPRLVK
EYWETIKLTKFSLLTISAMFGIGYLTRYSGLDATLGLAFAQTGVLYPLFGTMLGWLGVALTGSDTASNVLFGGLQKTTAE
QLGLSPVLMAAANSSGGVMGKMIDAQSIVVASTATKWYGHEGDILRYVFFHSIALAILVGIFVTLQAYVEPFTHMVIH

Specific function: Transports glycolate across the membrane. Can also transport L-lactate and D-lactate. Seems to be driven by a proton motive force [H]

COG id: COG1620

COG function: function code C; L-lactate permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lactate permease family [H]

Homologues:

Organism=Escherichia coli, GI1789347, Length=562, Percent_Identity=45.373665480427, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1790031, Length=554, Percent_Identity=43.3212996389892, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003804 [H]

Pfam domain/function: PF02652 Lactate_perm [H]

EC number: NA

Molecular weight: Translated: 58959; Mature: 58959

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactate [Periplasm]; Proton [Periplasm]; glycolate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + lactate [Periplasm] = Proton [Cytoplasm] + lactate [Cytoplasm] Proton [Periplasm] + glycolate [Periplasm] = Proton [Cytoplasm] + glycolate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 11283302; 11785976 [H]