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The map label for this gene is alaXM [H]

Identifier: 73542031

GI number: 73542031

Start: 2586575

End: 2587288

Strand: Direct

Name: alaXM [H]

Synonym: Reut_A2345

Alternate gene names: 73542031

Gene position: 2586575-2587288 (Clockwise)

Preceding gene: 73542030

Following gene: 73542032

Centisome position: 67.95

GC content: 66.53

Gene sequence:

>714_bases
ATGACCGTCACCCGCAAACGCTTCGACGACGATCCCTATCTCGACCATTGCAGCGCCACGGTGCTGGCAGTGAGCGATGC
CGGCATCGAACTCGACGAGACCATCTGCTATGCGCGCAGCGGCGGCCAGGCCGGCGACACGGCCACGCTGACGCTGGAGG
ACGGGCGCACCATGTCGATTACCGACACCGTCTACGCCGACGACCGCGCCCGCATTCTGCATGTCCCCGCGGAAGGCACG
GCGCTGCCGCGCGTAGGCGAGCGTGTCACGGTCACGATCGACTGGGAGCGACGCCACCGGCTGATGCGCCTGCACACCTG
CCTGCACCTGCTGGGCTCGCTGATCCCCGTGCCTGTGACGGGCTGCGGTATCTCGCCGGATTCGGCACGTATCGATTTCG
ACCTGCCCGAATCGACGCTCGACAAGGCCGTGCTGACCGAGCAGCTCAACGCGCTGATCCGCGCCAATACGGCCGTGCGC
GTTACGCTGATCACGCCGCAGGAACTGGCCGCACAGCCGGAACTCGTGCGCACCATCGGCGCCGCGCCGCCGGCCGGTAC
CAGCAGCATCCGCATCATCGAGATTCCGGGTGTCGACCGCCAGCCTTGTGGCGGCACGCATGTGGCCAACACCAGCGAGA
TCGGCGCTGTGGTGGTGACCAAGATCGAGAAAAAAAGCCGCACCAACCGGCGCGTGGTCGTCAATTTCGCCTGA

Upstream 100 bases:

>100_bases
CCCGCCAGTGCTTATCGCCGAAATCGAAACGCCGCAGGGCATCCGCACCCTGCGCTAGAACCCGACATCAACAAGAACAA
GACCACGGAGACACCCACCG

Downstream 100 bases:

>100_bases
CCGCCATGACCGGAGATTTCCTGACCGGCCTGTCGGCCGCGCAATTCCTTGCACTGGTTACGCTGCTGGCGGTGGGTTCG
TTCACGCCCGGGCCTAACAC

Product: Ala-tRNA(Pro) hydrolase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MTVTRKRFDDDPYLDHCSATVLAVSDAGIELDETICYARSGGQAGDTATLTLEDGRTMSITDTVYADDRARILHVPAEGT
ALPRVGERVTVTIDWERRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALIRANTAVR
VTLITPQELAAQPELVRTIGAAPPAGTSSIRIIEIPGVDRQPCGGTHVANTSEIGAVVVTKIEKKSRTNRRVVVNFA

Sequences:

>Translated_237_residues
MTVTRKRFDDDPYLDHCSATVLAVSDAGIELDETICYARSGGQAGDTATLTLEDGRTMSITDTVYADDRARILHVPAEGT
ALPRVGERVTVTIDWERRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALIRANTAVR
VTLITPQELAAQPELVRTIGAAPPAGTSSIRIIEIPGVDRQPCGGTHVANTSEIGAVVVTKIEKKSRTNRRVVVNFA
>Mature_236_residues
TVTRKRFDDDPYLDHCSATVLAVSDAGIELDETICYARSGGQAGDTATLTLEDGRTMSITDTVYADDRARILHVPAEGTA
LPRVGERVTVTIDWERRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALIRANTAVRV
TLITPQELAAQPELVRTIGAAPPAGTSSIRIIEIPGVDRQPCGGTHVANTSEIGAVVVTKIEKKSRTNRRVVVNFA

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=207, Percent_Identity=28.5024154589372, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI217416402, Length=207, Percent_Identity=28.5024154589372, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 25600; Mature: 25468

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTRKRFDDDPYLDHCSATVLAVSDAGIELDETICYARSGGQAGDTATLTLEDGRTMSI
CCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCEEEEECCCCCCCCEEEEEECCCCEEEE
TDTVYADDRARILHVPAEGTALPRVGERVTVTIDWERRHRLMRLHTCLHLLGSLIPVPVT
EEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEE
GCGISPDSARIDFDLPESTLDKAVLTEQLNALIRANTAVRVTLITPQELAAQPELVRTIG
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCHHHHHHHC
AAPPAGTSSIRIIEIPGVDRQPCGGTHVANTSEIGAVVVTKIEKKSRTNRRVVVNFA
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEC
>Mature Secondary Structure 
TVTRKRFDDDPYLDHCSATVLAVSDAGIELDETICYARSGGQAGDTATLTLEDGRTMSI
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECCEEEEECCCCCCCCEEEEEECCCCEEEE
TDTVYADDRARILHVPAEGTALPRVGERVTVTIDWERRHRLMRLHTCLHLLGSLIPVPVT
EEEEEECCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEE
GCGISPDSARIDFDLPESTLDKAVLTEQLNALIRANTAVRVTLITPQELAAQPELVRTIG
CCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHCCHHHHHHHC
AAPPAGTSSIRIIEIPGVDRQPCGGTHVANTSEIGAVVVTKIEKKSRTNRRVVVNFA
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]