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The map label for this gene is leuC

Identifier: 73542000

GI number: 73542000

Start: 2552227

End: 2553636

Strand: Reverse

Name: leuC

Synonym: Reut_A2314

Alternate gene names: 73542000

Gene position: 2553636-2552227 (Counterclockwise)

Preceding gene: 73542001

Following gene: 73541999

Centisome position: 67.09

GC content: 64.89

Gene sequence:

>1410_bases
ATGGCCAAGACGCTCTACGACAAACTCTGGGATGACCACACCGTCCACGTCGAGGAAGACGGCACTACGCTGCTCTACAT
CGATCGTCATCTGCTGCATGAAGTGACCAGCCCGCAGGCGTTCGAAGGCCTGAAGATAGCAGAGCGTCCGGTGTGGCGCA
TCAGCGCGAACCTGGCGGTGTCGGACCACAACGTGCCGACCACTGACCGCAGCGAAGGTATCGCCGACCCGGTGTCGAAG
CTGCAGGTCGATACGCTGGACGCAAACTGCGACAGTTTCGGCATCACCCAGTTCAAGATGAACGATCATCGCCAGGGCAT
CGTGCACGTGATCGGGCCGGAGCAGGGCGCCACGCTGCCGGGCATGACGGTGGTGTGCGGCGACTCGCATACCTCCACGC
ACGGTGCATTCGGTGCGCTCGCGCATGGCATCGGTACGTCGGAAGTCGAGCACGTGCTGGCCACGCAGACGCTGCTGGGC
AAGAAGGCCAAGAACATGCTGGTCAAGGTGGAAGGCAAGCTGCCGCGCGGCTGCACCGCCAAGGACATCGTGCTGGCCAT
CATCGGCAAGATCGGCACGGCCGGCGGTACCGGCTACACGATCGAATTCGCCGGGTCGGCCATCCGCGACCTGACCATGG
AAGGCCGCATGACGGTCTGCAACATGGCCATCGAAGCCGGCGCACGTGCGGGGCTGGTGGCCGTGGACAATGTCACGCTG
GAATACGTGAAGGGTCGTCCGTATGCGCCGCAGGGCGTAGAGTGGGACCAGGCCGTGGCCTACTGGAGCACGCTGCATTC
GGACGCCGGCGCCAGTTTCGACCAGGTGATCGAGCTGCGTGCCGAGGACATCCGCCCGCAGGTGACCTGGGGCACGTCGC
CCGAAATGGTCGTCAGCATCGAAGACCGCGTGCCGGATCCCGAAAAGGAAAAGGACCCGACCAGGCGCAACGCCATGGAG
CGCGCGCTCGAGTACATGAACCTGCAGCCCAACGTGCCGATGGAAAGCATCAACGTGGACAAGGTGTTCATCGGTTCCTG
CACCAACAGCCGCATCGAAGACATGCGCGCCGCCGCGTGGGTGGTGCAGAAGCTGGGCCGCAAGGTGGCTTCGAACGTGA
AGCTGGCGATGGTCGTGCCGGGTTCGGGCCTGGTCAAGGAACAGGCCGAGCGCGAAGGGCTCGACAAGGTCTTCAAGGCC
GCAGGCTTCGAATGGCGCGAGCCGGGTTGCTCGATGTGCCTGGCGATGAATGCCGATCGCCTGGAGCCGGGCGAGCGCTG
CGCGTCCACGTCGAACCGCAACTTCGAAGGCCGTCAGGGCGCGGGTGGCCGCACCCATCTGGTGAGCCCGGCGATGGCCG
CCGCGGCCGCCATCGAAGGCCACTTCGTCGACATCCGCAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
GGTAACGAGGCGGCCGGACCACACAAAGATCCCTGCAAACTACTTGCGCGGTACCGTGCGCCATTTCCGCCGGAATGGCA
CGCTGGCCGCCCCATGCATC

Downstream 100 bases:

>100_bases
GCGGAGTGCTGCAGATGAGAACCCTGATCGCTCTGCTGGCATGCTTCGGCATGCTGCAACTGGCCGGCTGCAACACCATG
GCCGGTCTGGGCAAGGACAC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKIAERPVWRISANLAVSDHNVPTTDRSEGIADPVSK
LQVDTLDANCDSFGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLG
KKAKNMLVKVEGKLPRGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDNVTL
EYVKGRPYAPQGVEWDQAVAYWSTLHSDAGASFDQVIELRAEDIRPQVTWGTSPEMVVSIEDRVPDPEKEKDPTRRNAME
RALEYMNLQPNVPMESINVDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKVFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Sequences:

>Translated_469_residues
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKIAERPVWRISANLAVSDHNVPTTDRSEGIADPVSK
LQVDTLDANCDSFGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLG
KKAKNMLVKVEGKLPRGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDNVTL
EYVKGRPYAPQGVEWDQAVAYWSTLHSDAGASFDQVIELRAEDIRPQVTWGTSPEMVVSIEDRVPDPEKEKDPTRRNAME
RALEYMNLQPNVPMESINVDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKVFKA
AGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
>Mature_468_residues
AKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKIAERPVWRISANLAVSDHNVPTTDRSEGIADPVSKL
QVDTLDANCDSFGITQFKMNDHRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGK
KAKNMLVKVEGKLPRGCTAKDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDNVTLE
YVKGRPYAPQGVEWDQAVAYWSTLHSDAGASFDQVIELRAEDIRPQVTWGTSPEMVVSIEDRVPDPEKEKDPTRRNAMER
ALEYMNLQPNVPMESINVDKVFIGSCTNSRIEDMRAAAWVVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKVFKAA
GFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=27.5482093663912, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI8659555, Length=403, Percent_Identity=26.7990074441687, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI41352693, Length=371, Percent_Identity=27.7628032345013, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786259, Length=468, Percent_Identity=64.957264957265, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=27.7929155313352, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=364, Percent_Identity=29.3956043956044, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=364, Percent_Identity=29.3956043956044, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=303, Percent_Identity=29.042904290429, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=26.6666666666667, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.8305084745763, Blast_Score=615, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=28.5318559556787, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=26.7605633802817, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=27.3480662983425, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=394, Percent_Identity=27.1573604060914, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=394, Percent_Identity=27.1573604060914, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161076999, Length=394, Percent_Identity=27.1573604060914, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28571643, Length=471, Percent_Identity=26.7515923566879, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24645686, Length=374, Percent_Identity=28.6096256684492, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI17137564, Length=384, Percent_Identity=25.2604166666667, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_CUPPJ (Q46YV7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296520.1
- ProteinModelPortal:   Q46YV7
- SMR:   Q46YV7
- GeneID:   3609020
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2314
- NMPDR:   fig|264198.3.peg.3044
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   REUT264198:REUT_A2314-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50826; Mature: 50695

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKIAERPVWRISANLAV
CCCHHHHHHCCCCEEEEECCCCEEEEECHHHHHHCCCCCHHCCCCCCCCCEEEEEEEEEE
SDHNVPTTDRSEGIADPVSKLQVDTLDANCDSFGITQFKMNDHRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGKKAKNMLVKVEGKLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDNVTL
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCHHEEHHHHHCCCCCCEEEECCEEE
EYVKGRPYAPQGVEWDQAVAYWSTLHSDAGASFDQVIELRAEDIRPQVTWGTSPEMVVSI
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEE
EDRVPDPEKEKDPTRRNAMERALEYMNLQPNVPMESINVDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKVFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
AKTLYDKLWDDHTVHVEEDGTTLLYIDRHLLHEVTSPQAFEGLKIAERPVWRISANLAV
CCHHHHHHCCCCEEEEECCCCEEEEECHHHHHHCCCCCHHCCCCCCCCCEEEEEEEEEE
SDHNVPTTDRSEGIADPVSKLQVDTLDANCDSFGITQFKMNDHRQGIVHVIGPEQGATLP
ECCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCC
GMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLGKKAKNMLVKVEGKLPRGCTA
CEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCH
KDIVLAIIGKIGTAGGTGYTIEFAGSAIRDLTMEGRMTVCNMAIEAGARAGLVAVDNVTL
HHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHCCCCHHEEHHHHHCCCCCCEEEECCEEE
EYVKGRPYAPQGVEWDQAVAYWSTLHSDAGASFDQVIELRAEDIRPQVTWGTSPEMVVSI
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCEEEEE
EDRVPDPEKEKDPTRRNAMERALEYMNLQPNVPMESINVDKVFIGSCTNSRIEDMRAAAW
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHH
VVQKLGRKVASNVKLAMVVPGSGLVKEQAEREGLDKVFKAAGFEWREPGCSMCLAMNADR
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCC
LEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAIEGHFVDIRKLG
CCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA