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The map label for this gene is folC [H]

Identifier: 73541988

GI number: 73541988

Start: 2538535

End: 2539845

Strand: Reverse

Name: folC [H]

Synonym: Reut_A2302

Alternate gene names: 73541988

Gene position: 2539845-2538535 (Counterclockwise)

Preceding gene: 73541989

Following gene: 73541987

Centisome position: 66.72

GC content: 64.61

Gene sequence:

>1311_bases
ATGCCTGTCTTCCACAACCTTCCCGAATGGCTCGCCCATCTCGAAACCGCCCATCCCGTGGGCATCGACATGGGCCTGAC
CCGTATCACGCGCGTCAAGGAAGCGCTGGGCCTGCGCATTGATGCCGTGGTGTTCACGGTCGGCGGTACCAACGGCAAAG
GCTCGACCTGCGCCATGCTGGAGCGCATCATGCTGGAAGCCGGCTACAAGGTGGGCTGCCATACCTCACCGCATCTGGTG
ACGTTCAACGAGCGCGCGCGCCTCAATGGCGAGATGGCGACGGATGCGCAACTGCTGCCGCACTTCGAGGCCGTGGAGCG
TGCCCGCACCAGCTTTGCAGACCCGATCAGCCTGACGTATTTCGAGTTCACCACGCTGGCGATCATCCATATGTTCGCGG
CGGCCGGGCTGGACGCGATGATCCTGGAAGTGGGCCTGGGTGGGCGACTCGATGCGGTCAACGTGATCGATACGGATTGT
GCCGTGATCACCAGCGTCGATATCGACCACACGCAGTACCTCGGCGACACGCGCGAGAAGATCGGCTTCGAGAAGGCTGG
CATTTTCCGTCCGGGCGTGCCGGCGATCTGTGGCGATCCGGTGCCGCCACAGTCGTTGATCGACCATGCCGAGGCGATCG
GCGCCGACCTGTGGCTGGTCGGGCGCGATTTCCGCCACCAGGCCGCCAAGGGTCAGGAGCGCCAACAGTGGGACTGGAGC
GGACGCGAGCGCAAGCTTAACGGGCTTGGCTATCCGGCGTTGCGCGGCGCGAATCAATTGCTGAACGCTTCGGCTGCACT
GGCAGCGCTGCAGGCGATGCGTGCGCAGTTGCCCGTGAGTGCGCAGGAAGTCCGCAACGGCCTGGCCTTCGTGGAGCTGC
CCGGGCGCTTCCAGGTGCTGCCGGGACGCCCGGCGGTGATCCTCGACGTGGCGCATAACCCGCATGCGGCCGCCACGCTG
GGCCAGAACCTGGAAAACATGGGCTTCTTCCGCTACACCTACGCGGTGTTCGGCGCCATGCAGGACAAGGATCTCGCGGG
TGTCCTGCGCCAGGTGGCCGACAAGGTGGATCACTGGTGCCTGTGCGACCTGCCGACCGAGCGCGCGGCAACTGCCGCCG
ATTTGCTGCAGAAGCTCGAAGAGGGCGGTTTCCAGCCGGGGCCGGATGCCACGGCGGCCTGTTTTTCCAGCCCCGAAGCC
GCTTACCGCGATGCCATCGAGAGAGCGACCGAGAATGATAGAATTCTGGTCTTCGGATCGTTCTATACCGTTGCCGGCGT
AATGGCATACCGCGCCACGCAGGCAAACTAG

Upstream 100 bases:

>100_bases
GCGCCTTTTGTTTTTCCTGGCGCCGAGGCAATGCCAGCTCCGGCCTTGACTTTCAGGCCGGCTGGCCAAATCTAGAAGCA
AAGCATTTCCACCCCCCTTC

Downstream 100 bases:

>100_bases
CGCAGCGCCGCACACGAAAGTGCGGCACAAGTTCGCGGCAATCGAGCGACAAACCAGCGGCCGGCACGGAGCGAATCCGA
CGGCCGCCGCACCGAACCAA

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPVFHNLPEWLAHLETAHPVGIDMGLTRITRVKEALGLRIDAVVFTVGGTNGKGSTCAMLERIMLEAGYKVGCHTSPHLV
TFNERARLNGEMATDAQLLPHFEAVERARTSFADPISLTYFEFTTLAIIHMFAAAGLDAMILEVGLGGRLDAVNVIDTDC
AVITSVDIDHTQYLGDTREKIGFEKAGIFRPGVPAICGDPVPPQSLIDHAEAIGADLWLVGRDFRHQAAKGQERQQWDWS
GRERKLNGLGYPALRGANQLLNASAALAALQAMRAQLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATL
GQNLENMGFFRYTYAVFGAMQDKDLAGVLRQVADKVDHWCLCDLPTERAATAADLLQKLEEGGFQPGPDATAACFSSPEA
AYRDAIERATENDRILVFGSFYTVAGVMAYRATQAN

Sequences:

>Translated_436_residues
MPVFHNLPEWLAHLETAHPVGIDMGLTRITRVKEALGLRIDAVVFTVGGTNGKGSTCAMLERIMLEAGYKVGCHTSPHLV
TFNERARLNGEMATDAQLLPHFEAVERARTSFADPISLTYFEFTTLAIIHMFAAAGLDAMILEVGLGGRLDAVNVIDTDC
AVITSVDIDHTQYLGDTREKIGFEKAGIFRPGVPAICGDPVPPQSLIDHAEAIGADLWLVGRDFRHQAAKGQERQQWDWS
GRERKLNGLGYPALRGANQLLNASAALAALQAMRAQLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATL
GQNLENMGFFRYTYAVFGAMQDKDLAGVLRQVADKVDHWCLCDLPTERAATAADLLQKLEEGGFQPGPDATAACFSSPEA
AYRDAIERATENDRILVFGSFYTVAGVMAYRATQAN
>Mature_435_residues
PVFHNLPEWLAHLETAHPVGIDMGLTRITRVKEALGLRIDAVVFTVGGTNGKGSTCAMLERIMLEAGYKVGCHTSPHLVT
FNERARLNGEMATDAQLLPHFEAVERARTSFADPISLTYFEFTTLAIIHMFAAAGLDAMILEVGLGGRLDAVNVIDTDCA
VITSVDIDHTQYLGDTREKIGFEKAGIFRPGVPAICGDPVPPQSLIDHAEAIGADLWLVGRDFRHQAAKGQERQQWDWSG
RERKLNGLGYPALRGANQLLNASAALAALQAMRAQLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATLG
QNLENMGFFRYTYAVFGAMQDKDLAGVLRQVADKVDHWCLCDLPTERAATAADLLQKLEEGGFQPGPDATAACFSSPEAA
YRDAIERATENDRILVFGSFYTVAGVMAYRATQAN

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=366, Percent_Identity=30.8743169398907, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI66932990, Length=366, Percent_Identity=30.8743169398907, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788654, Length=423, Percent_Identity=42.5531914893617, Blast_Score=295, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI17553148, Length=471, Percent_Identity=26.3269639065817, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17553150, Length=471, Percent_Identity=26.3269639065817, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71984923, Length=471, Percent_Identity=26.3269639065817, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323760, Length=363, Percent_Identity=28.3746556473829, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=174, Percent_Identity=37.9310344827586, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24641571, Length=149, Percent_Identity=36.9127516778523, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24581568, Length=150, Percent_Identity=34.6666666666667, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47307; Mature: 47176

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFHNLPEWLAHLETAHPVGIDMGLTRITRVKEALGLRIDAVVFTVGGTNGKGSTCAML
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHH
ERIMLEAGYKVGCHTSPHLVTFNERARLNGEMATDAQLLPHFEAVERARTSFADPISLTY
HHHHHHCCCEECCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
FEFTTLAIIHMFAAAGLDAMILEVGLGGRLDAVNVIDTDCAVITSVDIDHTQYLGDTREK
HHHHHHHHHHHHHHHCHHHEEHEECCCCCCCEEEEECCCCEEEEECCCCHHHHHCCHHHH
IGFEKAGIFRPGVPAICGDPVPPQSLIDHAEAIGADLWLVGRDFRHQAAKGQERQQWDWS
CCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCHHCCCCC
GRERKLNGLGYPALRGANQLLNASAALAALQAMRAQLPVSAQEVRNGLAFVELPGRFQVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCEEEC
PGRPAVILDVAHNPHAAATLGQNLENMGFFRYTYAVFGAMQDKDLAGVLRQVADKVDHWC
CCCCEEEEEECCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
LCDLPTERAATAADLLQKLEEGGFQPGPDATAACFSSPEAAYRDAIERATENDRILVFGS
EECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEC
FYTVAGVMAYRATQAN
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PVFHNLPEWLAHLETAHPVGIDMGLTRITRVKEALGLRIDAVVFTVGGTNGKGSTCAML
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHH
ERIMLEAGYKVGCHTSPHLVTFNERARLNGEMATDAQLLPHFEAVERARTSFADPISLTY
HHHHHHCCCEECCCCCCCEEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHH
FEFTTLAIIHMFAAAGLDAMILEVGLGGRLDAVNVIDTDCAVITSVDIDHTQYLGDTREK
HHHHHHHHHHHHHHHCHHHEEHEECCCCCCCEEEEECCCCEEEEECCCCHHHHHCCHHHH
IGFEKAGIFRPGVPAICGDPVPPQSLIDHAEAIGADLWLVGRDFRHQAAKGQERQQWDWS
CCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHHHHHHCCCCHHCCCCC
GRERKLNGLGYPALRGANQLLNASAALAALQAMRAQLPVSAQEVRNGLAFVELPGRFQVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEECCCEEEC
PGRPAVILDVAHNPHAAATLGQNLENMGFFRYTYAVFGAMQDKDLAGVLRQVADKVDHWC
CCCCEEEEEECCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEE
LCDLPTERAATAADLLQKLEEGGFQPGPDATAACFSSPEAAYRDAIERATENDRILVFGS
EECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEC
FYTVAGVMAYRATQAN
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]