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The map label for this gene is dat [H]

Identifier: 73541912

GI number: 73541912

Start: 2454990

End: 2455838

Strand: Reverse

Name: dat [H]

Synonym: Reut_A2225

Alternate gene names: 73541912

Gene position: 2455838-2454990 (Counterclockwise)

Preceding gene: 73541914

Following gene: 73541911

Centisome position: 64.52

GC content: 63.72

Gene sequence:

>849_bases
ATGTCCGAGCCTCTCGTCTATCTCAACGGCGATCTGACCCCCTTGTCCGAAGCCCGCATCCCCGTCCTGGATCGCGGTTT
CATCTTCGGTGATGGCATTTACGAGGTCATCCCGATCTACAACGGCAAGCCGTTCCGGGGCGCTCAGCACCTGGCACGCC
TCGCGCGTAGCCTGGCGGCCATCGGTATTCCCGATCCGCACGACGCGTCCGAATGGCTGGCGCTGATCGCGCGCGTGGCG
GAGGCGAACGGGCTGTCCGACCAGATGAGCTATATCCAGGTCACCCGGGGCGTGGCACGGCGCACACATGCGTTTCCAAC
GGAAGTCACGCCGACCGTGCTGATCATGACCAATCCGATGGTGCTGCCGGTTGCGGAGGCGGTGGCGCAGGGGGTGGCGT
GCGTGACGATGGAGGATCGGCGCTGGCTCAATTGCCAGATCAAGTCGACGTCGCTGCTCGGTAATGTGCTGGCCGCGCAA
TATGCGGCGGAGCATAGCGCAACCGAGGCGATCCAGTTTCGCGATGGCTGGCTCACGGAAGCCTCGGCGTCCAATGTCTG
GGTGGTGCGTAGCGGCCGAGTATCGGCGCCACCCAAGGACAACCTGATCCTGGAGGGCATCCGCTACGGGTTGTTGGAAG
AGTTGTGCGCCGCGCTGGACATCTCGCTGGAAACCCGCCGCATCTCGCGCGAGGAGGTCTTCGGTGCGGACGAGGTATTG
CTGTCGTCGGCGAGCAAGGAACTATTGCCAGTGGTGTCCATTGATGGACAGACGATTGGAGCAGGAGCGCCCGGCCCGGT
CTTTCGCAAGCTCTACGACGCTTACCAGGCCGCCAAGGCCGCGCTTTGA

Upstream 100 bases:

>100_bases
GAAGCGACCCGTCCGGAGCTCCGAAGCCTTATGCGCATATTGCATAAGCCATCCCACGCGCCTGTGAAAGAATCGGACAT
CCCCTCAGCAAGCACTTACC

Downstream 100 bases:

>100_bases
GCGCACGGGCATGCCCGTGCTAGCATGACCGCCGAACCGCTTCCGGGAGACAGCCATGGCCAAGCGCGAGCGCGGCCGGC
GCGCCTTGCAGGACGAGGTA

Product: branched chain amino acid: 2-keto-4-methylthiobutyrate aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSEPLVYLNGDLTPLSEARIPVLDRGFIFGDGIYEVIPIYNGKPFRGAQHLARLARSLAAIGIPDPHDASEWLALIARVA
EANGLSDQMSYIQVTRGVARRTHAFPTEVTPTVLIMTNPMVLPVAEAVAQGVACVTMEDRRWLNCQIKSTSLLGNVLAAQ
YAAEHSATEAIQFRDGWLTEASASNVWVVRSGRVSAPPKDNLILEGIRYGLLEELCAALDISLETRRISREEVFGADEVL
LSSASKELLPVVSIDGQTIGAGAPGPVFRKLYDAYQAAKAAL

Sequences:

>Translated_282_residues
MSEPLVYLNGDLTPLSEARIPVLDRGFIFGDGIYEVIPIYNGKPFRGAQHLARLARSLAAIGIPDPHDASEWLALIARVA
EANGLSDQMSYIQVTRGVARRTHAFPTEVTPTVLIMTNPMVLPVAEAVAQGVACVTMEDRRWLNCQIKSTSLLGNVLAAQ
YAAEHSATEAIQFRDGWLTEASASNVWVVRSGRVSAPPKDNLILEGIRYGLLEELCAALDISLETRRISREEVFGADEVL
LSSASKELLPVVSIDGQTIGAGAPGPVFRKLYDAYQAAKAAL
>Mature_281_residues
SEPLVYLNGDLTPLSEARIPVLDRGFIFGDGIYEVIPIYNGKPFRGAQHLARLARSLAAIGIPDPHDASEWLALIARVAE
ANGLSDQMSYIQVTRGVARRTHAFPTEVTPTVLIMTNPMVLPVAEAVAQGVACVTMEDRRWLNCQIKSTSLLGNVLAAQY
AAEHSATEAIQFRDGWLTEASASNVWVVRSGRVSAPPKDNLILEGIRYGLLEELCAALDISLETRRISREEVFGADEVLL
SSASKELLPVVSIDGQTIGAGAPGPVFRKLYDAYQAAKAAL

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=281, Percent_Identity=25.9786476868327, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 30483; Mature: 30352

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLVYLNGDLTPLSEARIPVLDRGFIFGDGIYEVIPIYNGKPFRGAQHLARLARSLAA
CCCCEEEECCCCCCCCCCCCCEEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IGIPDPHDASEWLALIARVAEANGLSDQMSYIQVTRGVARRTHAFPTEVTPTVLIMTNPM
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
VLPVAEAVAQGVACVTMEDRRWLNCQIKSTSLLGNVLAAQYAAEHSATEAIQFRDGWLTE
HHHHHHHHHCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
ASASNVWVVRSGRVSAPPKDNLILEGIRYGLLEELCAALDISLETRRISREEVFGADEVL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHH
LSSASKELLPVVSIDGQTIGAGAPGPVFRKLYDAYQAAKAAL
HHCCCHHCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEPLVYLNGDLTPLSEARIPVLDRGFIFGDGIYEVIPIYNGKPFRGAQHLARLARSLAA
CCCEEEECCCCCCCCCCCCCEEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
IGIPDPHDASEWLALIARVAEANGLSDQMSYIQVTRGVARRTHAFPTEVTPTVLIMTNPM
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
VLPVAEAVAQGVACVTMEDRRWLNCQIKSTSLLGNVLAAQYAAEHSATEAIQFRDGWLTE
HHHHHHHHHCCEEEEEECCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
ASASNVWVVRSGRVSAPPKDNLILEGIRYGLLEELCAALDISLETRRISREEVFGADEVL
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHH
LSSASKELLPVVSIDGQTIGAGAPGPVFRKLYDAYQAAKAAL
HHCCCHHCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]