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The map label for this gene is rnhA [H]

Identifier: 73541876

GI number: 73541876

Start: 2417285

End: 2417722

Strand: Direct

Name: rnhA [H]

Synonym: Reut_A2189

Alternate gene names: 73541876

Gene position: 2417285-2417722 (Clockwise)

Preceding gene: 73541860

Following gene: 73541877

Centisome position: 63.5

GC content: 63.93

Gene sequence:

>438_bases
ATGCAAGAAGTAATCATCTATTCCGATGGCGCCTGCAAAGGCAATCCCGGCCGTGGCGGCTGGGGCGCGGTACTCGTGGC
CGGCACGAACGAAAAAGAGCTGTTCGGCGGCGAGGCCAACACCACCAACAACCGCATGGAGATGACCGCGGTCATCGAGG
CGCTGCGCGCGCTCAAGCGGCCCTGCACCGTGCAGGTGTACACCGACTCGCAGTACGTGCAGAAAGGCATCAGCGAATGG
CTGCCGGGCTGGAAGGCGCGCGGCTGGAAGACGGCCGACAAAAAGCCCGTGAAGAACGCGGACCTGTGGCAGGAACTCGA
TACGCTCGTACAGCCGCACAAGATCACCTGGCACTGGGTGCGCGGACATAATGGCCACCCTGGCAACGAGCGCGCCGATG
CGCTTGCCAACCGCGGCGTTGCATCGCTCGCCTCCTGA

Upstream 100 bases:

>100_bases
GGGCACGCACGGCAAGACGCCGGGCGACGAAAGCTGACCCGGCCGGCGCGCACACATAACTCCTGTGGCAGCCCTTGCGG
GCTGCCCTGACAAACGACAC

Downstream 100 bases:

>100_bases
CCTCGCCCTGCCGTCCGCTAGAATGCGCGCCATGCGACAAATCGTTCTCGACACCGAAACCACTGGCCTCAATGCCGCCA
CGGGCGACCGCCTGATCGAA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MQEVIIYSDGACKGNPGRGGWGAVLVAGTNEKELFGGEANTTNNRMEMTAVIEALRALKRPCTVQVYTDSQYVQKGISEW
LPGWKARGWKTADKKPVKNADLWQELDTLVQPHKITWHWVRGHNGHPGNERADALANRGVASLAS

Sequences:

>Translated_145_residues
MQEVIIYSDGACKGNPGRGGWGAVLVAGTNEKELFGGEANTTNNRMEMTAVIEALRALKRPCTVQVYTDSQYVQKGISEW
LPGWKARGWKTADKKPVKNADLWQELDTLVQPHKITWHWVRGHNGHPGNERADALANRGVASLAS
>Mature_145_residues
MQEVIIYSDGACKGNPGRGGWGAVLVAGTNEKELFGGEANTTNNRMEMTAVIEALRALKRPCTVQVYTDSQYVQKGISEW
LPGWKARGWKTADKKPVKNADLWQELDTLVQPHKITWHWVRGHNGHPGNERADALANRGVASLAS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=36.1702127659575, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1786408, Length=136, Percent_Identity=58.0882352941177, Blast_Score=169, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=37.1428571428571, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17505482, Length=130, Percent_Identity=33.0769230769231, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI45552509, Length=148, Percent_Identity=37.8378378378378, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17137476, Length=148, Percent_Identity=37.8378378378378, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 15981; Mature: 15981

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEVIIYSDGACKGNPGRGGWGAVLVAGTNEKELFGGEANTTNNRMEMTAVIEALRALKR
CCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCC
PCTVQVYTDSQYVQKGISEWLPGWKARGWKTADKKPVKNADLWQELDTLVQPHKITWHWV
CCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
RGHNGHPGNERADALANRGVASLAS
ECCCCCCCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MQEVIIYSDGACKGNPGRGGWGAVLVAGTNEKELFGGEANTTNNRMEMTAVIEALRALKR
CCEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCC
PCTVQVYTDSQYVQKGISEWLPGWKARGWKTADKKPVKNADLWQELDTLVQPHKITWHWV
CCEEEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEE
RGHNGHPGNERADALANRGVASLAS
ECCCCCCCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA