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The map label for this gene is rlmE

Identifier: 73541859

GI number: 73541859

Start: 2395457

End: 2396134

Strand: Reverse

Name: rlmE

Synonym: Reut_A2171

Alternate gene names: 73541859

Gene position: 2396134-2395457 (Counterclockwise)

Preceding gene: 73541861

Following gene: 73541858

Centisome position: 62.95

GC content: 57.82

Gene sequence:

>678_bases
ATGGCCAAAGGCAAGTCCAAGAACAAGTTCAACCAGTCGTGGCTGCACGACCACATCAACGACCCGTATGTGAAGATGGC
GCAGCGCGAAGGTTATCGTGCGCGCGCAGCCTACAAGCTCAAGGAAATCGACGAGCAGGACAAGCTGATCCAGCCCGGCC
AAGTCATCGTGGATCTGGGCGCCGCGCCGGGAAGCTGGAGCCAGTACGCGCGCAACAAGCTGGCTGCGTCGCCGCGCGCA
ACTGGCGGCAAGCCTGACGGCGCCGTAGTGGCCATTGATATCCTCCCAATGGAGCCTGTCGCGGACGTCACGTTTATCCA
GGGCGATTTTCGCGAGGAAGACGTATTCCGCCAGTTGGAGGAAGTGGTCATGGCCGCTTCTGGTGGCGCAAAAATCGATC
TTGTATTGTCGGACATGGCCCCCAATCTGTCTGGTGTAGCCTCAGCCGACTCTGCGCGCATTGAATATCTGTGTGATCTG
GCCCTGGATTTTGCCCAGACCCACCTGAAGCCGGACGGCGCATTGCTGGTAAAGTGTTTCCACGGCAGTGGCTACAGCCA
GATCGTCGAGAAGTTCAAACGACAGTTCAAGGTGGTCGCCCCACGCAAGCCCAAGGCGTCGCGCGACAAATCCTCTGAAA
CTTTTATCCTTGGGCGTTACCTGAAAGTGGTGAATTGA

Upstream 100 bases:

>100_bases
AAACCAGTACAGGTTGGCGGCAAAAAACGGCAAAACAGGCAAGAAATTGCGGCGCGCCCGCCTTCGCGGGGACGGCAGAC
AGGAAACAGGCGTAATCAGA

Downstream 100 bases:

>100_bases
CGGATCCGGCAGGTGCAGGGCAGTTACTGGCTCGGGGCTATCGGCTTCATAGTTTCGCTGTTTCCCCTTCGGGGCGGGCA
CTGCATTACAATGCAGCTAT

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MAKGKSKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRA
TGGKPDGAVVAIDILPMEPVADVTFIQGDFREEDVFRQLEEVVMAASGGAKIDLVLSDMAPNLSGVASADSARIEYLCDL
ALDFAQTHLKPDGALLVKCFHGSGYSQIVEKFKRQFKVVAPRKPKASRDKSSETFILGRYLKVVN

Sequences:

>Translated_225_residues
MAKGKSKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRA
TGGKPDGAVVAIDILPMEPVADVTFIQGDFREEDVFRQLEEVVMAASGGAKIDLVLSDMAPNLSGVASADSARIEYLCDL
ALDFAQTHLKPDGALLVKCFHGSGYSQIVEKFKRQFKVVAPRKPKASRDKSSETFILGRYLKVVN
>Mature_224_residues
AKGKSKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRAT
GGKPDGAVVAIDILPMEPVADVTFIQGDFREEDVFRQLEEVVMAASGGAKIDLVLSDMAPNLSGVASADSARIEYLCDLA
LDFAQTHLKPDGALLVKCFHGSGYSQIVEKFKRQFKVVAPRKPKASRDKSSETFILGRYLKVVN

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=217, Percent_Identity=35.0230414746544, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=34.8717948717949, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=34.8717948717949, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=34.8717948717949, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI194097365, Length=198, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789569, Length=214, Percent_Identity=43.4579439252336, Blast_Score=187, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17554650, Length=197, Percent_Identity=34.5177664974619, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17553474, Length=225, Percent_Identity=30.6666666666667, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=197, Percent_Identity=30.9644670050761, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=197, Percent_Identity=30.9644670050761, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=197, Percent_Identity=30.9644670050761, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319535, Length=200, Percent_Identity=33, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=203, Percent_Identity=32.0197044334975, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=110, Percent_Identity=38.1818181818182, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356387, Length=221, Percent_Identity=36.6515837104072, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24647580, Length=197, Percent_Identity=32.994923857868, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648639, Length=199, Percent_Identity=32.6633165829146, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859957, Length=197, Percent_Identity=29.9492385786802, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_CUPPJ (Q46Z98)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296379.1
- ProteinModelPortal:   Q46Z98
- SMR:   Q46Z98
- GeneID:   3609485
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2171
- NMPDR:   fig|264198.3.peg.2983
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK896994
- BioCyc:   REUT264198:REUT_A2171-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24783; Mature: 24651

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 64-64 BINDING 66-66 BINDING 93-93 BINDING 109-109 BINDING 138-138

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKSKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIQPGQVIVDLG
CCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECC
AAPGSWSQYARNKLAASPRATGGKPDGAVVAIDILPMEPVADVTFIQGDFREEDVFRQLE
CCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
EVVMAASGGAKIDLVLSDMAPNLSGVASADSARIEYLCDLALDFAQTHLKPDGALLVKCF
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
HGSGYSQIVEKFKRQFKVVAPRKPKASRDKSSETFILGRYLKVVN
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEHHHHHHHCC
>Mature Secondary Structure 
AKGKSKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIQPGQVIVDLG
CCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCCEEEEECC
AAPGSWSQYARNKLAASPRATGGKPDGAVVAIDILPMEPVADVTFIQGDFREEDVFRQLE
CCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHH
EVVMAASGGAKIDLVLSDMAPNLSGVASADSARIEYLCDLALDFAQTHLKPDGALLVKCF
HHHHHCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
HGSGYSQIVEKFKRQFKVVAPRKPKASRDKSSETFILGRYLKVVN
CCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA