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The map label for this gene is phaB [H]

Identifier: 73541824

GI number: 73541824

Start: 2356098

End: 2356940

Strand: Reverse

Name: phaB [H]

Synonym: Reut_A2136

Alternate gene names: 73541824

Gene position: 2356940-2356098 (Counterclockwise)

Preceding gene: 73541825

Following gene: 73541823

Centisome position: 61.92

GC content: 63.58

Gene sequence:

>843_bases
ATGACAACTAACAATGCCAGCCCCGAAGCTGGACATATCATGCAGACCCGTACGGTAGACCTAGACGGGCAGGTACTCAG
GGTCGGCATCCGGCCCGGCAGCGGCGATCTCCCGCCGCTGCTTATGTTCAATGGCATCGGCGCAAACCTTGAACTCGTCG
AACCCTTCGTCGATGCGCTGCCGGATGTGGAAGTGATCATCTTCGACATCCCCGGTGCCGGCGGCTCTCCGGCGCCCCTG
TTCCCCTATCGTTTCTCCAATCTTTGCGTGATGGCAGACAAGCTGCTGTCGCACCTCGGCTATGCCGGACCGGTCGATAC
GCTCGGCGTTTCATGGGGCGGCGCACTGGCACAGGAATTCGCGCGGCTGTATCCGCAGCGCTGCCGCCGCCTGGTGCTGG
CTGCCACATCGCCTGGCGTGCTCATGGTGCCGGGCAGCCCATCCGTCCTCACCAAGATGATCGGCATTCGACGTTACACC
GATGCGGAGTACCTGCGGCGAGTCGGTGGGGACATCTACGGCGGCGCCTTTCGCGACGACCCGTCATTGCTCGATGTCCA
TGGGGGGCATATGCGTCCGCCGCGCGGGCGCGGCTACCTGTATCAACTGCTGGCCGCATGGGGCTGGAGCAGCCTGCCGT
GGTTGCGGGCGCTGAAGCAGCGCACGCTGGTCTTGCATGGCAGCGATGACCCGATCGTGCCGCTGATCAACGCAAAGATC
CTGTCCGGTTGCATTCCGGACTCGGCTCTGCATGTGTTCCACGACGATGGCCATCTCTTTCTTCTGACCGCGGCACGCGA
ATCGGCGCGGGTGGTGGGAGAGTTCCTGCGAGAAGGCCGCTGA

Upstream 100 bases:

>100_bases
CGTCGAAGAGGCAAAAGGGATCCTGCGTCCCAGGCAGTAAGCGCAAGGAAGCATGGCCCCGGTGGCCATGCTTCACCGCC
AACCTCTCCGGGACGACTTG

Downstream 100 bases:

>100_bases
CACGGCGCCCCGTCCATGCGCCCCGTCCATGACGGGGCGTTTTCGCTTTCAGGCCCGGTAGCGAGGGGGCGGTGCGCCGC
AATGCCGGCAAGCGAAAAAT

Product: Poly(3-hydroxyalkanoate) depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTTNNASPEAGHIMQTRTVDLDGQVLRVGIRPGSGDLPPLLMFNGIGANLELVEPFVDALPDVEVIIFDIPGAGGSPAPL
FPYRFSNLCVMADKLLSHLGYAGPVDTLGVSWGGALAQEFARLYPQRCRRLVLAATSPGVLMVPGSPSVLTKMIGIRRYT
DAEYLRRVGGDIYGGAFRDDPSLLDVHGGHMRPPRGRGYLYQLLAAWGWSSLPWLRALKQRTLVLHGSDDPIVPLINAKI
LSGCIPDSALHVFHDDGHLFLLTAARESARVVGEFLREGR

Sequences:

>Translated_280_residues
MTTNNASPEAGHIMQTRTVDLDGQVLRVGIRPGSGDLPPLLMFNGIGANLELVEPFVDALPDVEVIIFDIPGAGGSPAPL
FPYRFSNLCVMADKLLSHLGYAGPVDTLGVSWGGALAQEFARLYPQRCRRLVLAATSPGVLMVPGSPSVLTKMIGIRRYT
DAEYLRRVGGDIYGGAFRDDPSLLDVHGGHMRPPRGRGYLYQLLAAWGWSSLPWLRALKQRTLVLHGSDDPIVPLINAKI
LSGCIPDSALHVFHDDGHLFLLTAARESARVVGEFLREGR
>Mature_279_residues
TTNNASPEAGHIMQTRTVDLDGQVLRVGIRPGSGDLPPLLMFNGIGANLELVEPFVDALPDVEVIIFDIPGAGGSPAPLF
PYRFSNLCVMADKLLSHLGYAGPVDTLGVSWGGALAQEFARLYPQRCRRLVLAATSPGVLMVPGSPSVLTKMIGIRRYTD
AEYLRRVGGDIYGGAFRDDPSLLDVHGGHMRPPRGRGYLYQLLAAWGWSSLPWLRALKQRTLVLHGSDDPIVPLINAKIL
SGCIPDSALHVFHDDGHLFLLTAARESARVVGEFLREGR

Specific function: PHA depolymerase is in fact a lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR011942 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: NA

Molecular weight: Translated: 30355; Mature: 30224

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNNASPEAGHIMQTRTVDLDGQVLRVGIRPGSGDLPPLLMFNGIGANLELVEPFVDAL
CCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHC
PDVEVIIFDIPGAGGSPAPLFPYRFSNLCVMADKLLSHLGYAGPVDTLGVSWGGALAQEF
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHH
ARLYPQRCRRLVLAATSPGVLMVPGSPSVLTKMIGIRRYTDAEYLRRVGGDIYGGAFRDD
HHHHHHHHHHEEEEECCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PSLLDVHGGHMRPPRGRGYLYQLLAAWGWSSLPWLRALKQRTLVLHGSDDPIVPLINAKI
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCEEEEHHHHH
LSGCIPDSALHVFHDDGHLFLLTAARESARVVGEFLREGR
HHCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTNNASPEAGHIMQTRTVDLDGQVLRVGIRPGSGDLPPLLMFNGIGANLELVEPFVDAL
CCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHC
PDVEVIIFDIPGAGGSPAPLFPYRFSNLCVMADKLLSHLGYAGPVDTLGVSWGGALAQEF
CCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHH
ARLYPQRCRRLVLAATSPGVLMVPGSPSVLTKMIGIRRYTDAEYLRRVGGDIYGGAFRDD
HHHHHHHHHHEEEEECCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
PSLLDVHGGHMRPPRGRGYLYQLLAAWGWSSLPWLRALKQRTLVLHGSDDPIVPLINAKI
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCCEEEEHHHHH
LSGCIPDSALHVFHDDGHLFLLTAARESARVVGEFLREGR
HHCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1989978 [H]