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The map label for this gene is gltX

Identifier: 73541811

GI number: 73541811

Start: 2340644

End: 2342050

Strand: Reverse

Name: gltX

Synonym: Reut_A2123

Alternate gene names: 73541811

Gene position: 2342050-2340644 (Counterclockwise)

Preceding gene: 73541813

Following gene: 73541804

Centisome position: 61.53

GC content: 64.25

Gene sequence:

>1407_bases
ATGACCCAACGCGTCCGCACCCGTTTCGCGCCGAGCCCGACCGGCTTTATCCACCTCGGCAATATTCGATCCGCGCTTTA
TCCGTGGGCGTTCGCCCGCCGGATGAAGGGCGATTTCGTGCTGCGCATCGAAGATACCGATGTCGAGCGTTCCTCGCAGG
AAGCCGTCGACGTGATCCTCGAAGGCATGGCGTGGCTCGGCCTGGATATCGACGAAGGCCCGTTCTACCAGATGCAGCGC
ATGGACCGCTACCGCGAGGTGCTGGCCCAGATGGTCGACAGCGGCCTGGCCTACCCGTGCTATATGAGCACGGAAGAACT
GGACGCGCTGCGCGAAGCCCAGCGCGAGCGCGGCGAAAAGCCGCGCTACAACGGTTTCTGGCGCCCCGAGCCGGGCAAGG
TCTTGCCGCAGCCGCCGGCCGGCGTGAAGCCGGTGCTGCGTTTCCGCAATCCGCTTGACGGCAGCGTAGTCTGGGACGAC
GCGGTCAAGGGCCGTATCGAGATCTCGAACGAGGAGTTGGACGACCTCGTCATTGCGCGGCCGGACGGCACGCCCACCTA
CAACTTCTGCGTGGTGGTCGACGATCTCGACATGAAGATCACGCACGTGATCCGTGGTGACGACCACGTCAACAATACGC
CGCGTCAGATCAATATCATCCGCGCGCTTGGCGGCACACAGCCCGTCTACGCGCACTTGCCCACGGTGCTCAACGAGAGC
GGCGAGAAGATGAGCAAGCGTCATGGCGCCATGAGCGTGACTGGCTATCGCGATGAAGGCTATCTGCCGGAAGCCGTGGT
GAACTACCTGGCCCGCCTGGGCTGGGCGCATGGCGACGCGGAAATCTTCAGCCGCGAGCAGTTTGTGGAGTGGTTCGACC
TCGAACACCTGGGCAAGTCGCCGGCGCAGTACAACCCGGAAAAGCTGGCCTGGCTGAACAACCACTACATCAAGGTGGGC
GACAATGCGCGCCTGGCCGAGCTGACGCGTCCATTCATCGAGGCGCTCGGCGGCAAGGTGGAGGGTGCGGACCTGGTTGG
GGTGATCGCACTCGTCAAGGACCGGGCTAATACGCTCAAGGACGTTGCGCAGACGGCGCTGCTGTTCTACCGCGGCGAGC
CACAGGCTGATGCTGCCCTGAAGGCCGAGCACCTGACGCCGGAAATCCAGCCGGCACTGAAGGCGCTGGCGACTCAACTG
GGCGCCTTGCCCGAATGGAAGCGCGAAGCCATCAGCGCTACGTTCAAGGCGGTGCTTGCGGAGTTTGGGCTGAAGATGCC
CAAGCTGGCCATGCCAGTGCGCCTGCTGGTCGCTGGCCAGTTGCAGACGCCGAGCATCGACGCGGTGCTCGAGCTGTTCG
GCCGGGAAGCTGTGCTGCGCCGTATCGGCGCGGGCGTCGAAGCGTAA

Upstream 100 bases:

>100_bases
GGCGGGCGTGGGCCAGCGGGTTCGCTTGCTATAATGCAGCACCGACATATTCCAGCCTGCGCGCGTCCGCGCGGGCTTTT
TTGTTTGTTCCCCGACCGCC

Downstream 100 bases:

>100_bases
AAAGCTGCGAAAAACTCCTGGCAGGGGCTTGCGCTCCGGCCCGGAGTTCGCCATAATCTCGTTCTCCCTGCAGCGCGGTC
AGTGATCAAGCGGGCGGTCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARRMKGDFVLRIEDTDVERSSQEAVDVILEGMAWLGLDIDEGPFYQMQR
MDRYREVLAQMVDSGLAYPCYMSTEELDALREAQRERGEKPRYNGFWRPEPGKVLPQPPAGVKPVLRFRNPLDGSVVWDD
AVKGRIEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGTQPVYAHLPTVLNES
GEKMSKRHGAMSVTGYRDEGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVG
DNARLAELTRPFIEALGGKVEGADLVGVIALVKDRANTLKDVAQTALLFYRGEPQADAALKAEHLTPEIQPALKALATQL
GALPEWKREAISATFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGREAVLRRIGAGVEA

Sequences:

>Translated_468_residues
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARRMKGDFVLRIEDTDVERSSQEAVDVILEGMAWLGLDIDEGPFYQMQR
MDRYREVLAQMVDSGLAYPCYMSTEELDALREAQRERGEKPRYNGFWRPEPGKVLPQPPAGVKPVLRFRNPLDGSVVWDD
AVKGRIEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGTQPVYAHLPTVLNES
GEKMSKRHGAMSVTGYRDEGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVG
DNARLAELTRPFIEALGGKVEGADLVGVIALVKDRANTLKDVAQTALLFYRGEPQADAALKAEHLTPEIQPALKALATQL
GALPEWKREAISATFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGREAVLRRIGAGVEA
>Mature_467_residues
TQRVRTRFAPSPTGFIHLGNIRSALYPWAFARRMKGDFVLRIEDTDVERSSQEAVDVILEGMAWLGLDIDEGPFYQMQRM
DRYREVLAQMVDSGLAYPCYMSTEELDALREAQRERGEKPRYNGFWRPEPGKVLPQPPAGVKPVLRFRNPLDGSVVWDDA
VKGRIEISNEELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGTQPVYAHLPTVLNESG
EKMSKRHGAMSVTGYRDEGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVGD
NARLAELTRPFIEALGGKVEGADLVGVIALVKDRANTLKDVAQTALLFYRGEPQADAALKAEHLTPEIQPALKALATQLG
ALPEWKREAISATFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGREAVLRRIGAGVEA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=29.8387096774194, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1788743, Length=465, Percent_Identity=48.1720430107527, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=34.6938775510204, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1786895, Length=119, Percent_Identity=27.7310924369748, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=31.198347107438, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=487, Percent_Identity=28.7474332648871, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321192, Length=221, Percent_Identity=27.6018099547511, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=107, Percent_Identity=28.9719626168224, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=30.8641975308642, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CUPPJ (Q46ZE6)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_296331.1
- ProteinModelPortal:   Q46ZE6
- SMR:   Q46ZE6
- GeneID:   3610852
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2123
- NMPDR:   fig|264198.3.peg.2935
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- BioCyc:   REUT264198:REUT_A2123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52323; Mature: 52191

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRVRTRFAPSPTGFIHLGNIRSALYPWAFARRMKGDFVLRIEDTDVERSSQEAVDVIL
CCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
EGMAWLGLDIDEGPFYQMQRMDRYREVLAQMVDSGLAYPCYMSTEELDALREAQRERGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCC
PRYNGFWRPEPGKVLPQPPAGVKPVLRFRNPLDGSVVWDDAVKGRIEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGTQPVYAHLPTVLNES
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
GEKMSKRHGAMSVTGYRDEGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYNPEKLAWLNNHYIKVGDNARLAELTRPFIEALGGKVEGADLVGVIALVKDRANTLK
CCCCCCHHEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DVAQTALLFYRGEPQADAALKAEHLTPEIQPALKALATQLGALPEWKREAISATFKAVLA
HHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGREAVLRRIGAGVEA
HHCCCCCCHHHCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQRVRTRFAPSPTGFIHLGNIRSALYPWAFARRMKGDFVLRIEDTDVERSSQEAVDVIL
CCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHH
EGMAWLGLDIDEGPFYQMQRMDRYREVLAQMVDSGLAYPCYMSTEELDALREAQRERGEK
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHCCCC
PRYNGFWRPEPGKVLPQPPAGVKPVLRFRNPLDGSVVWDDAVKGRIEISNEELDDLVIAR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCEEEECCCCCCCEEEEC
PDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGTQPVYAHLPTVLNES
CCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
GEKMSKRHGAMSVTGYRDEGYLPEAVVNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKS
HHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
PAQYNPEKLAWLNNHYIKVGDNARLAELTRPFIEALGGKVEGADLVGVIALVKDRANTLK
CCCCCCHHEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
DVAQTALLFYRGEPQADAALKAEHLTPEIQPALKALATQLGALPEWKREAISATFKAVLA
HHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGREAVLRRIGAGVEA
HHCCCCCCHHHCHHHEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA