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The map label for this gene is bbuR [H]

Identifier: 73541598

GI number: 73541598

Start: 2094725

End: 2095729

Strand: Direct

Name: bbuR [H]

Synonym: Reut_A1909

Alternate gene names: 73541598

Gene position: 2094725-2095729 (Clockwise)

Preceding gene: 73541592

Following gene: 73541599

Centisome position: 55.03

GC content: 64.88

Gene sequence:

>1005_bases
ATGGAGACTTCAGTTAGCGCAAAAGTGTATATTCTGCTATACCAGCTATCCACTTTATGGATAGATGACCATCAACCAGG
CAAAGTGAACCTCAAGCAACTGGAAACCGTGGCCCTGGTGGCTGAACTGGGCTCGCTGTCGCGGGCGGCGCGCGCGCTGG
GCACGGCGCAGTCGCTGGTCAGCCGCGCCATCGCGCAGATCGAAGCGGAATGGGGTGACCGGCTGTTCGAGCGCACGGGA
CGCGGCGTAGTCCTGTCCGAATTCGGCACGCGGATCTTTCCGGAGATCGAACTGCTGCTGGCCCAGTCGGCGCGCCTGCA
CGACGAAGTGCGCAACGCGGCGGGCGTGCCGGTCGGGATGGTGCGGATCGGCGTACTGCCGTCCATGACGCGCCGCCTGA
TTCCCGCTCTGTTCGCCGACCTGCGCGATACCGCGCCGGGGGTCCGGCTGCGTGTCGCGGAAGGCTTCAGCGGCCAGCTC
GACGAACAGCTCGATTCCGGCCACCTCGACCTTGCGGTCATCAACCGCTATGGTCCAAGCCCGCGCCAGAACGAGGATGT
ACTCGCCACCGTGGCGAATTATCTGGTCAGCGCGCCGGGTCATCCGCTCACGGCAGGGCCTGCCGTCGATTTCCGACGGC
TAGACGGGGTGCCACTGGTATTGCCGCCTGCGCCCAACGGGTTGCGCCTGATCCTGGACCAGCACGCACGGCAACAGGGC
GTAGCGCTGGACGTTGCGCTGGAGGTCGAATCCCTGAACGCGATGAAGGAGGTTGCATTGTCAGGGCACGCCCTGACCAT
ACTGCCGCAACTGGCCGTGGATGATGAAGTTGGCGCCGGGCGCCTCGCGGCCGCGGAAATCGTCAAGCCGCAACTGCCAC
GAGCCATCAGCCTGGGGGTCACGCAGCATCACCCGCTGTCTCGTGCGGCCAGGGTGGTACTGTCACGCCTGCATGACCTG
GTCCCGCACGTAACGGCCCGCAACTGGTCAGGCAAGGCGAAGTAA

Upstream 100 bases:

>100_bases
AGCGACTGGCGCATGGAGCGCCCTATCGTCGAAAAACTTCGCATGTTGTCTCCATATCGTTTGAGCAACCCTTCTCTGCA
GCGAAGGATGCACCCAGTGT

Downstream 100 bases:

>100_bases
CGCTCGCCGCAAGGCCCGCATACGCGGGAAGCCCATGGCCATTGCAGCGGAGCCTGCACCGGCGAAGTTCGAATTCGACC
GCAACGAGAGGAGACAAATG

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
METSVSAKVYILLYQLSTLWIDDHQPGKVNLKQLETVALVAELGSLSRAARALGTAQSLVSRAIAQIEAEWGDRLFERTG
RGVVLSEFGTRIFPEIELLLAQSARLHDEVRNAAGVPVGMVRIGVLPSMTRRLIPALFADLRDTAPGVRLRVAEGFSGQL
DEQLDSGHLDLAVINRYGPSPRQNEDVLATVANYLVSAPGHPLTAGPAVDFRRLDGVPLVLPPAPNGLRLILDQHARQQG
VALDVALEVESLNAMKEVALSGHALTILPQLAVDDEVGAGRLAAAEIVKPQLPRAISLGVTQHHPLSRAARVVLSRLHDL
VPHVTARNWSGKAK

Sequences:

>Translated_334_residues
METSVSAKVYILLYQLSTLWIDDHQPGKVNLKQLETVALVAELGSLSRAARALGTAQSLVSRAIAQIEAEWGDRLFERTG
RGVVLSEFGTRIFPEIELLLAQSARLHDEVRNAAGVPVGMVRIGVLPSMTRRLIPALFADLRDTAPGVRLRVAEGFSGQL
DEQLDSGHLDLAVINRYGPSPRQNEDVLATVANYLVSAPGHPLTAGPAVDFRRLDGVPLVLPPAPNGLRLILDQHARQQG
VALDVALEVESLNAMKEVALSGHALTILPQLAVDDEVGAGRLAAAEIVKPQLPRAISLGVTQHHPLSRAARVVLSRLHDL
VPHVTARNWSGKAK
>Mature_334_residues
METSVSAKVYILLYQLSTLWIDDHQPGKVNLKQLETVALVAELGSLSRAARALGTAQSLVSRAIAQIEAEWGDRLFERTG
RGVVLSEFGTRIFPEIELLLAQSARLHDEVRNAAGVPVGMVRIGVLPSMTRRLIPALFADLRDTAPGVRLRVAEGFSGQL
DEQLDSGHLDLAVINRYGPSPRQNEDVLATVANYLVSAPGHPLTAGPAVDFRRLDGVPLVLPPAPNGLRLILDQHARQQG
VALDVALEVESLNAMKEVALSGHALTILPQLAVDDEVGAGRLAAAEIVKPQLPRAISLGVTQHHPLSRAARVVLSRLHDL
VPHVTARNWSGKAK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=293, Percent_Identity=26.962457337884, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788297, Length=308, Percent_Identity=26.6233766233766, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=237, Percent_Identity=31.2236286919831, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082132, Length=260, Percent_Identity=26.5384615384615, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=279, Percent_Identity=26.1648745519713, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI145693105, Length=252, Percent_Identity=24.6031746031746, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790399, Length=270, Percent_Identity=26.2962962962963, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788748, Length=291, Percent_Identity=24.7422680412371, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=263, Percent_Identity=26.9961977186312, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789204, Length=253, Percent_Identity=24.901185770751, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=267, Percent_Identity=25.0936329588015, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789440, Length=174, Percent_Identity=29.8850574712644, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789639, Length=125, Percent_Identity=27.2, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35964; Mature: 35964

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSVSAKVYILLYQLSTLWIDDHQPGKVNLKQLETVALVAELGSLSRAARALGTAQSLV
CCCCCCCEEEEEEEEHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAIAQIEAEWGDRLFERTGRGVVLSEFGTRIFPEIELLLAQSARLHDEVRNAAGVPVGM
HHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VRIGVLPSMTRRLIPALFADLRDTAPGVRLRVAEGFSGQLDEQLDSGHLDLAVINRYGPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCEEEEEEECCCCC
PRQNEDVLATVANYLVSAPGHPLTAGPAVDFRRLDGVPLVLPPAPNGLRLILDQHARQQG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHCC
VALDVALEVESLNAMKEVALSGHALTILPQLAVDDEVGAGRLAAAEIVKPQLPRAISLGV
CEEEEEEEHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCHHHHHHHHCCCCCCCEECCC
TQHHPLSRAARVVLSRLHDLVPHVTARNWSGKAK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
METSVSAKVYILLYQLSTLWIDDHQPGKVNLKQLETVALVAELGSLSRAARALGTAQSLV
CCCCCCCEEEEEEEEHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAIAQIEAEWGDRLFERTGRGVVLSEFGTRIFPEIELLLAQSARLHDEVRNAAGVPVGM
HHHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VRIGVLPSMTRRLIPALFADLRDTAPGVRLRVAEGFSGQLDEQLDSGHLDLAVINRYGPS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCEEEEEEECCCCC
PRQNEDVLATVANYLVSAPGHPLTAGPAVDFRRLDGVPLVLPPAPNGLRLILDQHARQQG
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCCCCHHHHHHHHHHHCC
VALDVALEVESLNAMKEVALSGHALTILPQLAVDDEVGAGRLAAAEIVKPQLPRAISLGV
CEEEEEEEHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCHHHHHHHHCCCCCCCEECCC
TQHHPLSRAARVVLSRLHDLVPHVTARNWSGKAK
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9524276; 12910271 [H]