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The map label for this gene is rluB [H]

Identifier: 73541573

GI number: 73541573

Start: 2062493

End: 2064577

Strand: Direct

Name: rluB [H]

Synonym: Reut_A1884

Alternate gene names: 73541573

Gene position: 2062493-2064577 (Clockwise)

Preceding gene: 73541572

Following gene: 73541578

Centisome position: 54.18

GC content: 70.79

Gene sequence:

>2085_bases
ATGACCGACAGCAATTCGCCGAAGCGCAAGACGCTCGGCATCAAGCCGCCCGACGGCACCGCCACCCGCAAGCCGGCCGG
CAACCGGCCCGTGCGCGTCTCCGACCTGAACCGCAAGCGCGTGCAGGCCGTGGCCGAAGGGATCCGGCGCGCCCAGGGCG
GCGCTGGCGCCAAGTCCGGCAAGGGCGGGATGCCTCCGGCCGACCCGGCAGCCGGCCGCGCTCCGCGGCCTGCGCGCGGC
GACAGCGATCGCCCGCCTCGTCAAGGCCGCCCCGCCGATGGTGAGCGCCAGGCGCGTCCGCGCCGCTTTGATGACGACCA
GTCGCGCCCTCGCCGTTTCGGCGACGATGACAACCGCCCCCGTCGATTCGGCAATGACGACCGCGCTCCGCGCAGGTTCG
GCGATGATGAAGCAAGGCCGCGCCGCTTCAGCGACGACAACCGTCCGCAGCGCCCGCGACGCCCTGAAGGCGGCGAACAG
CCGCAGCGCCGCTTCCGCGATGACGAATCGCGGCCGCGTCGTTTCGGTGACGATGAGGCACGCCCCCGCCGCTTCAGCGA
CGACAACCGCCTGCAGCGTCCGCGACGCCCCGAAGGTGGCGAACAACCGCAGCGCCGCTTCCGCGATGACGAATCGCGTC
CGCGTCGTTTCGGCGACGATGAGGCACGCCCTCGCCGCTTCAGCGACGACAACCGCCCGCAGCGCCCGCGACGTCCCGAA
GGTGGCGAACAGCCGCAGCCCCGCTTCCGCGATGACGAGTCGCGTCCGCGCCGTTTCGGCGACGATGAGGCACGCCCTCG
CCGCTTCAGCGACGACAACCGCCCGCAGCGCCCGCGACGTCCCGAAGGTGGCGAACAGCCGCAGCGCCGCTTCCGCGATG
ACGAGTCGCGGCCGCGCCGTTTCGGCAACGATGAGGCACGCCCTCGCCGCTTCAGCGACGACAACCGCCCGCAGCGCCCG
CGACGTCCCGAAGGTGGCGAACAGCCGCAGCGCCGCTTCCGCGATGACGAATCGCGGCCGCGCCGTTTCGGCGACGATGA
GGCACGCCCCCGCCGCTTCAGCGACGACAACCGTCCGCAGCGCCCGCGACGCCCCGAAGGTGGCGAACAACCGCAGCGCC
GCTTCCGCGATGACGAATCGCGGCCGCGCCGTTTCGGCGATGACGACCGCCAGCGCCGCGCACCGGACGCCGGTGCCCGC
CGGGGCGATGCACCGGCCCGACGCTTCAGCGACACCGCCGACCGCGCGCGCCCGGTCCGTGCCGAAAGGCAACCAGCCCA
TTCCAGCGGCGACGCCCCTGAGACCAGCCACGACGATGGCCTGATCCGCCTGTCCAAGCGCATGTCCGAACTGGGCCTGT
GCTCGCGTCGCGAAGCCGATGAATGGATTCCGCGCGGCTGGGTAATCGTCGACGGCAAGCCCGTGACCGAACTCGGCTCG
CGCGTGCGTGCCGATGCGAATATCGAGATCCACCAGGCCGCGCGTTCCGAACAAGGCGAGCGTGTCACGGTACTGCTGCA
CAAGCCCATGGGCTATGTCTCCGGCCAGGCCGAAGACGGCTACGAGCCCGCGGCCGTGCTGTTCACGGCGGAGAACCAGT
GGTCGGAAGACCCCACGCGCAAGCGCTTCGCGCCGTGGCAGCGCAAGAGCCTGGCGCCTGCCGGCCGTCTCGATATCGAC
TCCACCGGCCTGCTTGTGCTGACGCAGGATGGCCGCGTGGCGCGCGCCCTGATCGGCGAAGACTCCAACGTCGAGAAGGA
ATACCTGGTGCGCGTGCAGTGGCACTCGCCGGACGGCGTGGTCGAGCGTAATGTCAGCGAGGTCTTCCCTGCTGACAAGC
TCGAACTGCTGCGCCATGGCCTATCGCTCGACGGCGTGGTCCTGAAGCCGGCCAAGGTGAGCTGGCAGAACGAGGAGCAA
CTGCGGTTCGTGCTGCGCGAAGGCCGCAAGCGCCAGATCCGCCGCATGTGCGAACAGGTTGGCCTGGAGGTGGTTGGCCT
CAAGCGCGTGCGCATGGGCCGCATCGTGCTGGGTGACCTGCCGCCGGGGCAGTGGCGCTTCCTGGGTCCGTTCGAGAAGT
TCTGA

Upstream 100 bases:

>100_bases
ACGGCAGCCGTCTTGCCGCCGACAACCGATTGCAGATTCAGCTTGAACAGGACTTGCTCGCCGCGCTGGAAATCTGATCC
GGCCGCGCAGCATTGATGAT

Downstream 100 bases:

>100_bases
GAATCGGCATCGCATCGCCGGCGCACGTCGCCGGCGAGGTCGCAAAAGAAACGGGGCGCCAGTTGGCGCCCCGTTTCTTT
TGTGTCCATGCCGCGCTTCA

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MTDSNSPKRKTLGIKPPDGTATRKPAGNRPVRVSDLNRKRVQAVAEGIRRAQGGAGAKSGKGGMPPADPAAGRAPRPARG
DSDRPPRQGRPADGERQARPRRFDDDQSRPRRFGDDDNRPRRFGNDDRAPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQ
PQRRFRDDESRPRRFGDDEARPRRFSDDNRLQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPE
GGEQPQPRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGNDEARPRRFSDDNRPQRP
RRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDDRQRRAPDAGAR
RGDAPARRFSDTADRARPVRAERQPAHSSGDAPETSHDDGLIRLSKRMSELGLCSRREADEWIPRGWVIVDGKPVTELGS
RVRADANIEIHQAARSEQGERVTVLLHKPMGYVSGQAEDGYEPAAVLFTAENQWSEDPTRKRFAPWQRKSLAPAGRLDID
STGLLVLTQDGRVARALIGEDSNVEKEYLVRVQWHSPDGVVERNVSEVFPADKLELLRHGLSLDGVVLKPAKVSWQNEEQ
LRFVLREGRKRQIRRMCEQVGLEVVGLKRVRMGRIVLGDLPPGQWRFLGPFEKF

Sequences:

>Translated_694_residues
MTDSNSPKRKTLGIKPPDGTATRKPAGNRPVRVSDLNRKRVQAVAEGIRRAQGGAGAKSGKGGMPPADPAAGRAPRPARG
DSDRPPRQGRPADGERQARPRRFDDDQSRPRRFGDDDNRPRRFGNDDRAPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQ
PQRRFRDDESRPRRFGDDEARPRRFSDDNRLQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPE
GGEQPQPRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGNDEARPRRFSDDNRPQRP
RRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDDRQRRAPDAGAR
RGDAPARRFSDTADRARPVRAERQPAHSSGDAPETSHDDGLIRLSKRMSELGLCSRREADEWIPRGWVIVDGKPVTELGS
RVRADANIEIHQAARSEQGERVTVLLHKPMGYVSGQAEDGYEPAAVLFTAENQWSEDPTRKRFAPWQRKSLAPAGRLDID
STGLLVLTQDGRVARALIGEDSNVEKEYLVRVQWHSPDGVVERNVSEVFPADKLELLRHGLSLDGVVLKPAKVSWQNEEQ
LRFVLREGRKRQIRRMCEQVGLEVVGLKRVRMGRIVLGDLPPGQWRFLGPFEKF
>Mature_693_residues
TDSNSPKRKTLGIKPPDGTATRKPAGNRPVRVSDLNRKRVQAVAEGIRRAQGGAGAKSGKGGMPPADPAAGRAPRPARGD
SDRPPRQGRPADGERQARPRRFDDDQSRPRRFGDDDNRPRRFGNDDRAPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQP
QRRFRDDESRPRRFGDDEARPRRFSDDNRLQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEG
GEQPQPRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGNDEARPRRFSDDNRPQRPR
RPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDDRQRRAPDAGARR
GDAPARRFSDTADRARPVRAERQPAHSSGDAPETSHDDGLIRLSKRMSELGLCSRREADEWIPRGWVIVDGKPVTELGSR
VRADANIEIHQAARSEQGERVTVLLHKPMGYVSGQAEDGYEPAAVLFTAENQWSEDPTRKRFAPWQRKSLAPAGRLDIDS
TGLLVLTQDGRVARALIGEDSNVEKEYLVRVQWHSPDGVVERNVSEVFPADKLELLRHGLSLDGVVLKPAKVSWQNEEQL
RFVLREGRKRQIRRMCEQVGLEVVGLKRVRMGRIVLGDLPPGQWRFLGPFEKF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4503509, Length=290, Percent_Identity=38.6206896551724, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1790453, Length=250, Percent_Identity=34, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI1788510, Length=183, Percent_Identity=32.7868852459016, Blast_Score=76, Evalue=9e-15,
Organism=Escherichia coli, GI1787524, Length=260, Percent_Identity=31.9230769230769, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081838, Length=172, Percent_Identity=34.3023255813954, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 80371; Mature: 80240

Theoretical pI: Translated: 11.59; Mature: 11.59

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSNSPKRKTLGIKPPDGTATRKPAGNRPVRVSDLNRKRVQAVAEGIRRAQGGAGAKSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCC
KGGMPPADPAAGRAPRPARGDSDRPPRQGRPADGERQARPRRFDDDQSRPRRFGDDDNRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCC
RRFGNDDRAPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
RPRRFSDDNRLQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPE
CCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGEQPQPRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHH
FGNDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPRRPEGGEQPQRRFRDDESRPRRFGDDDRQRRAPDAGARRGDAPARRFSDTADRARPVR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
AERQPAHSSGDAPETSHDDGLIRLSKRMSELGLCSRREADEWIPRGWVIVDGKPVTELGS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCHHHHHH
RVRADANIEIHQAARSEQGERVTVLLHKPMGYVSGQAEDGYEPAAVLFTAENQWSEDPTR
HHCCCCCEEHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
KRFAPWQRKSLAPAGRLDIDSTGLLVLTQDGRVARALIGEDSNVEKEYLVRVQWHSPDGV
HHCCCHHHHHCCCCCCCCCCCCCEEEEECCCCEEHHHHCCCCCCCCEEEEEEEECCCCCH
VERNVSEVFPADKLELLRHGLSLDGVVLKPAKVSWQNEEQLRFVLREGRKRQIRRMCEQV
HHCCHHHHCCHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLEVVGLKRVRMGRIVLGDLPPGQWRFLGPFEKF
CCEEEEHHHHHHCCEEEECCCCCCEEECCCHHCC
>Mature Secondary Structure 
TDSNSPKRKTLGIKPPDGTATRKPAGNRPVRVSDLNRKRVQAVAEGIRRAQGGAGAKSG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCC
KGGMPPADPAAGRAPRPARGDSDRPPRQGRPADGERQARPRRFDDDQSRPRRFGDDDNRP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCC
RRFGNDDRAPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCC
RPRRFSDDNRLQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPE
CCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGEQPQPRFRDDESRPRRFGDDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHH
FGNDEARPRRFSDDNRPQRPRRPEGGEQPQRRFRDDESRPRRFGDDEARPRRFSDDNRPQ
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
RPRRPEGGEQPQRRFRDDESRPRRFGDDDRQRRAPDAGARRGDAPARRFSDTADRARPVR
CCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCC
AERQPAHSSGDAPETSHDDGLIRLSKRMSELGLCSRREADEWIPRGWVIVDGKPVTELGS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCCCHHHHHH
RVRADANIEIHQAARSEQGERVTVLLHKPMGYVSGQAEDGYEPAAVLFTAENQWSEDPTR
HHCCCCCEEHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHH
KRFAPWQRKSLAPAGRLDIDSTGLLVLTQDGRVARALIGEDSNVEKEYLVRVQWHSPDGV
HHCCCHHHHHCCCCCCCCCCCCCEEEEECCCCEEHHHHCCCCCCCCEEEEEEEECCCCCH
VERNVSEVFPADKLELLRHGLSLDGVVLKPAKVSWQNEEQLRFVLREGRKRQIRRMCEQV
HHCCHHHHCCHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLEVVGLKRVRMGRIVLGDLPPGQWRFLGPFEKF
CCEEEEHHHHHHCCEEEECCCCCCEEECCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]