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The map label for this gene is purT [H]

Identifier: 73541489

GI number: 73541489

Start: 1965991

End: 1967232

Strand: Direct

Name: purT [H]

Synonym: Reut_A1800

Alternate gene names: 73541489

Gene position: 1965991-1967232 (Clockwise)

Preceding gene: 73541487

Following gene: 73541492

Centisome position: 51.65

GC content: 67.79

Gene sequence:

>1242_bases
ATGCGGGCTTTCCCCGCCACACGAGCCACGACGCCCATGACCACCATCGGAACTCCCCTCTCCCCTTCGGCCACCAAGGT
CATGCTGCTCGGCTCCGGCGAGCTCGGCAAGGAAGTGCTGATTGCGCTGCAACGGCTGGGCGTCGAAACGATTGCCGTGG
ACCGCTACGACAACGCGCCCGGCCAGCAGGTGGCCCATCATGCGCGCACCATCGCCATGAGCGACCCGGAGCAGCTCAAG
GCACTGATCGAAGCGGAAAAGCCGCAACTGGTCGTGCCCGAGATCGAGGCCATTGCCACACCGATGCTCGAGTCGCTGGA
AGCCGCCGGCGTCGTGCGCGTGATTCCCACCGCGCGGGCAGCGCGCCTGACCATGGACCGCGAAGGCATCCGCCGGCTCG
CGGCCGAAACGCTGGGCCTGCCGACCAGCCCGTACAAGTTCTGCGATTCGCTTGAAGAACTGCAGACTGCGATCGATGGC
GGCATTGGCTATCCGTGCGTGGTCAAGCCGGTCATGAGCAGCTCGGGCAAGGGCCAGAGCAAGATCGACGGGCCCGAAGG
CGTCAAGGCGGCCTGGGACTACGCCATGGCGGGCGGGCGCGTGAGCCACGGCCGCGTGATCGTCGAAGGCTTTATCGACT
TCGATTACGAAATCACGCTGCTGACGGTGCGCGCACTGGGCGCTGACGGCAAGGTCGAAACGCAGTTCTGCACCCCGATC
GGCCACGTGCAGGTCAGCGGCGACTACGTCGAAAGCTGGCAGCCGCACCCGATGCACCCCGCGGCGCTCCAAACGGCGCA
GCACATTGCGCGCGCCGTCACGTCGGACCTCGGCGGCCAGGGCCTGTTCGGCGTGGAGCTGTTCGTCAAGGGCGAACAGG
TCTGGTTCAGCGAAGTCAGCCCGCGCCCGCACGATACCGGCATGGTCACCATGGCGACCCAATGGCAGAACGAGTTCGAG
CTGCATGCGCGCGCCATCCTGGGACTGCCGGTCAGCACGGCGCTGAAGAGCCCGGGCGCCAGCGCCGTCATCTACGGCGG
CGTGGATGCGCAGGGCGTGGTGTTCGACGGCGTGGATGCCGCCCTGCGCGTGCCGCAAACCGAGGTGCGCCTGTTCGGCA
AGCCGGAGAGCTTCGCCAAGCGCCGCATGGGTGTGGCGCTGGCCTATGCCGATGACGTGGACACCGCGCGCCAGCGCGCG
AAGGAAGCAGCCGCGTGCGTCAAGCCGCGCGTGGCGGGCTGA

Upstream 100 bases:

>100_bases
TGCCGTCATGAAACTTTTCGAGCTGTGAATTCAGCGGCGTGACACTGGCTCCGCGTTGGCGAGCCCGCGCGCGTGAAAAC
GGGTCCCGGCCTGCACTACA

Downstream 100 bases:

>100_bases
GGCCGAGGCCGAGGGCTACCGCATCGGCGTGGATAGCTGCACGGGCGGCGCCGCCTGTGCGGCCACGACCTGCGCTGCGG
CCAGCACGGGGTTGAGGCGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MRAFPATRATTPMTTIGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYDNAPGQQVAHHARTIAMSDPEQLK
ALIEAEKPQLVVPEIEAIATPMLESLEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQTAIDG
GIGYPCVVKPVMSSSGKGQSKIDGPEGVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRALGADGKVETQFCTPI
GHVQVSGDYVESWQPHPMHPAALQTAQHIARAVTSDLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMATQWQNEFE
LHARAILGLPVSTALKSPGASAVIYGGVDAQGVVFDGVDAALRVPQTEVRLFGKPESFAKRRMGVALAYADDVDTARQRA
KEAAACVKPRVAG

Sequences:

>Translated_413_residues
MRAFPATRATTPMTTIGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYDNAPGQQVAHHARTIAMSDPEQLK
ALIEAEKPQLVVPEIEAIATPMLESLEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQTAIDG
GIGYPCVVKPVMSSSGKGQSKIDGPEGVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRALGADGKVETQFCTPI
GHVQVSGDYVESWQPHPMHPAALQTAQHIARAVTSDLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMATQWQNEFE
LHARAILGLPVSTALKSPGASAVIYGGVDAQGVVFDGVDAALRVPQTEVRLFGKPESFAKRRMGVALAYADDVDTARQRA
KEAAACVKPRVAG
>Mature_413_residues
MRAFPATRATTPMTTIGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYDNAPGQQVAHHARTIAMSDPEQLK
ALIEAEKPQLVVPEIEAIATPMLESLEAAGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQTAIDG
GIGYPCVVKPVMSSSGKGQSKIDGPEGVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRALGADGKVETQFCTPI
GHVQVSGDYVESWQPHPMHPAALQTAQHIARAVTSDLGGQGLFGVELFVKGEQVWFSEVSPRPHDTGMVTMATQWQNEFE
LHARAILGLPVSTALKSPGASAVIYGGVDAQGVVFDGVDAALRVPQTEVRLFGKPESFAKRRMGVALAYADDVDTARQRA
KEAAACVKPRVAG

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=397, Percent_Identity=59.1939546599496, Blast_Score=430, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324702, Length=390, Percent_Identity=24.6153846153846, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 44047; Mature: 44047

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFPATRATTPMTTIGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYDNAP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GQQVAHHARTIAMSDPEQLKALIEAEKPQLVVPEIEAIATPMLESLEAAGVVRVIPTARA
HHHHHHHHHEEECCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEEECCCHH
ARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQTAIDGGIGYPCVVKPVMSSSGKGQS
HHEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
KIDGPEGVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRALGADGKVETQFCTPI
CCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCEEEEEECCC
GHVQVSGDYVESWQPHPMHPAALQTAQHIARAVTSDLGGQGLFGVELFVKGEQVWFSEVS
CEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCC
PRPHDTGMVTMATQWQNEFELHARAILGLPVSTALKSPGASAVIYGGVDAQGVVFDGVDA
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCHH
ALRVPQTEVRLFGKPESFAKRRMGVALAYADDVDTARQRAKEAAACVKPRVAG
HHCCCCHHEEEECCCHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRAFPATRATTPMTTIGTPLSPSATKVMLLGSGELGKEVLIALQRLGVETIAVDRYDNAP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCC
GQQVAHHARTIAMSDPEQLKALIEAEKPQLVVPEIEAIATPMLESLEAAGVVRVIPTARA
HHHHHHHHHEEECCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCEEEEECCCHH
ARLTMDREGIRRLAAETLGLPTSPYKFCDSLEELQTAIDGGIGYPCVVKPVMSSSGKGQS
HHEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
KIDGPEGVKAAWDYAMAGGRVSHGRVIVEGFIDFDYEITLLTVRALGADGKVETQFCTPI
CCCCCCCHHHHHHHHHCCCCCCCCEEEEEEEECCCEEEEEEEEEEECCCCCEEEEEECCC
GHVQVSGDYVESWQPHPMHPAALQTAQHIARAVTSDLGGQGLFGVELFVKGEQVWFSEVS
CEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCC
PRPHDTGMVTMATQWQNEFELHARAILGLPVSTALKSPGASAVIYGGVDAQGVVFDGVDA
CCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCEEECCCHH
ALRVPQTEVRLFGKPESFAKRRMGVALAYADDVDTARQRAKEAAACVKPRVAG
HHCCCCHHEEEECCCHHHHHHHHCEEEEECCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA