Click here to switch to the map view.

The map label for this gene is mphP [H]

Identifier: 73541393

GI number: 73541393

Start: 1838599

End: 1839657

Strand: Direct

Name: mphP [H]

Synonym: Reut_A1704

Alternate gene names: 73541393

Gene position: 1838599-1839657 (Clockwise)

Preceding gene: 73541392

Following gene: 73541394

Centisome position: 48.3

GC content: 64.97

Gene sequence:

>1059_bases
ATGACCTACCAACTGACCATCGAGCCCCTCGGGCAGACCATAGGCATCGAGGAAGGCCAAACCGTGCTCGACGCGTGCCT
GCGCGCCGGCGTGTGGTTGCCGCATGCGTGCGGCCACGGTCTATGCGGGACCTGCAAGGTGCAGACGCTCGAAGGCGAAC
TCGATCACGGCGAAGCATCGCCATTCGCGCTGATGGATTTCGAGCGCGAAGAAGCCAAGTGCCTGGCCTGCTGCGCGAAG
CCGATGTCCGACATGGTGATCGAGGTCGATATCGAGCCCGATCCCGATGCGCAGTGCCTGCCGCTGATGGATTTCACCGC
GGAAGTCGTGCGCATCGAGGCGCTGACGCCCACCATCAAGGGCGTGTTCCTGCGCATCGAAGGCGAGCCGCTGCAGTTCC
AGCCGGGCCAGTACGTCAACGTGTGGATCGGCAAGGAAGCCGCGCCACGCGCCTTCTCCATCGCCAGTGCGCCGACCGCG
GGCGAGATCGAACTGAACATCCGGCTCGTGCCCGGCGGCAGTGCGACGACCTATGTGCACGAACAACTTAGCGTGGGTGA
GCGCCTGCAGCTGTCGGGCCCGCTGGGCCGGTTCTTCGTGCGCAAGAGCGATCCGCGGCCGCTGGTGTTCATGGCGGGCG
GCTCGGGACTGTCAAGCCCGCGTTCGATGATCCTGGACCTGCTGGAATCGGGTGACCAACGCGAAATCGTGCTGGTGCAG
GGCGCACGCAATGCCGGCGAACTGTACTACCGCGATGCCTTCGAAGCGCTCGCACGGCAGCATGACAACTTCACCTACCT
GCCGGTGCTGTCGGGCGAGCCCGAGCAAAGCGACTGGGGCGGCGAGCGCGGCTATGTACACGATCTTGCAAGCCGTCATT
TCAGCCAAGACTTCCGCGGCTGGCGTGCCTACCTGTGCGGGCCGCCGCCGATGATCGAGGCTTGCATCGCCACGTTGATG
CAAGGCCGCCTGTTCGAGAAAGACATCTTCACCGAGAAGTTCCTGTCGGCAGCCGACGCGAGCCAGGCCACCCGCAGCCC
GCTGTTCCGCTCGCTCTGA

Upstream 100 bases:

>100_bases
CTGGCCGTCAACGGGCTCGGCCACAAGTCGCTACTGCGCCTGCGCACGCCGGAACTCCGCGGCATTGCCGGCTCGTGCAA
CTGACCTCACGGAGGCTGCC

Downstream 100 bases:

>100_bases
TACCGCAGCATCAGCCGCATTCCCACAAGGCCATAGGAGACAACCATGGACAAGATCGCCATCGACACCGTGCTCAACCG
CATCGAGGAAACCACGGGCC

Product: ferredoxin:oxidoreductase FAD/NAD(P)-binding:oxidoreductase FAD-binding region

Products: NA

Alternate protein names: Phenol 2-monooxygenase P5 component [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTYQLTIEPLGQTIGIEEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTLEGELDHGEASPFALMDFEREEAKCLACCAK
PMSDMVIEVDIEPDPDAQCLPLMDFTAEVVRIEALTPTIKGVFLRIEGEPLQFQPGQYVNVWIGKEAAPRAFSIASAPTA
GEIELNIRLVPGGSATTYVHEQLSVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQREIVLVQ
GARNAGELYYRDAFEALARQHDNFTYLPVLSGEPEQSDWGGERGYVHDLASRHFSQDFRGWRAYLCGPPPMIEACIATLM
QGRLFEKDIFTEKFLSAADASQATRSPLFRSL

Sequences:

>Translated_352_residues
MTYQLTIEPLGQTIGIEEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTLEGELDHGEASPFALMDFEREEAKCLACCAK
PMSDMVIEVDIEPDPDAQCLPLMDFTAEVVRIEALTPTIKGVFLRIEGEPLQFQPGQYVNVWIGKEAAPRAFSIASAPTA
GEIELNIRLVPGGSATTYVHEQLSVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQREIVLVQ
GARNAGELYYRDAFEALARQHDNFTYLPVLSGEPEQSDWGGERGYVHDLASRHFSQDFRGWRAYLCGPPPMIEACIATLM
QGRLFEKDIFTEKFLSAADASQATRSPLFRSL
>Mature_351_residues
TYQLTIEPLGQTIGIEEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTLEGELDHGEASPFALMDFEREEAKCLACCAKP
MSDMVIEVDIEPDPDAQCLPLMDFTAEVVRIEALTPTIKGVFLRIEGEPLQFQPGQYVNVWIGKEAAPRAFSIASAPTAG
EIELNIRLVPGGSATTYVHEQLSVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQREIVLVQG
ARNAGELYYRDAFEALARQHDNFTYLPVLSGEPEQSDWGGERGYVHDLASRHFSQDFRGWRAYLCGPPPMIEACIATLMQ
GRLFEKDIFTEKFLSAADASQATRSPLFRSL

Specific function: Probable electron transfer from NADPH, via FAD and the 2Fe-2S center, to the oxygenase activity site of the enzyme [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=186, Percent_Identity=29.5698924731183, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788903, Length=237, Percent_Identity=23.2067510548523, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787658, Length=213, Percent_Identity=25.8215962441315, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: =1.14.13.7 [H]

Molecular weight: Translated: 38672; Mature: 38540

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYQLTIEPLGQTIGIEEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTLEGELDHGEAS
CEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCC
PFALMDFEREEAKCLACCAKPMSDMVIEVDIEPDPDAQCLPLMDFTAEVVRIEALTPTIK
CEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEHHHHCEEEEEEEEECCCEE
GVFLRIEGEPLQFQPGQYVNVWIGKEAAPRAFSIASAPTAGEIELNIRLVPGGSATTYVH
EEEEEEECCCCEECCCCEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCEEEH
EQLSVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQREIVLVQ
HHHCCCCEEEECCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEE
GARNAGELYYRDAFEALARQHDNFTYLPVLSGEPEQSDWGGERGYVHDLASRHFSQDFRG
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
WRAYLCGPPPMIEACIATLMQGRLFEKDIFTEKFLSAADASQATRSPLFRSL
CEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
TYQLTIEPLGQTIGIEEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTLEGELDHGEAS
EEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCEEEEEEECCCCCCCCCC
PFALMDFEREEAKCLACCAKPMSDMVIEVDIEPDPDAQCLPLMDFTAEVVRIEALTPTIK
CEEEEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEHHHHCEEEEEEEEECCCEE
GVFLRIEGEPLQFQPGQYVNVWIGKEAAPRAFSIASAPTAGEIELNIRLVPGGSATTYVH
EEEEEEECCCCEECCCCEEEEEECCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCEEEH
EQLSVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQREIVLVQ
HHHCCCCEEEECCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEE
GARNAGELYYRDAFEALARQHDNFTYLPVLSGEPEQSDWGGERGYVHDLASRHFSQDFRG
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
WRAYLCGPPPMIEACIATLMQGRLFEKDIFTEKFLSAADASQATRSPLFRSL
CEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12732969 [H]