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The map label for this gene is phaD [H]

Identifier: 73541369

GI number: 73541369

Start: 1814244

End: 1815779

Strand: Direct

Name: phaD [H]

Synonym: Reut_A1680

Alternate gene names: 73541369

Gene position: 1814244-1815779 (Clockwise)

Preceding gene: 73541368

Following gene: 73541370

Centisome position: 47.66

GC content: 67.06

Gene sequence:

>1536_bases
GTGACCCACGTCCTGCTGCTTCCCATCCTTGTGCCGATGTTCGCCGGTGCACTGCTGACCGCCATGCCGATGCACTGGCT
GCGCGCGCAGCGCGTGGTGTGCATGGCCGCCACGCTGCTGCTGGTACCGGTTGCCGGGCTGCTTGTGGACCAGGCCGCGG
GCGGCAGCATCGCCGTCTACGCGCTCGGCAACTGGCAGGCGCCGTTCGGCATCGTCCTGCAACTCGACCGTCTTGGCGCG
ATGATGCTGGGGCTGACCGCGCTGCTGGCACCGGCGGCGCTCGCAGCTGCAGGGCCGGAACTCGTGCGTGCGGGCCGCCA
TTTCCACGCCTTGTTTCAGTTCCAGCTCATGGGACTGAACGGCGCGTTCCTGGCCGGCGACCTGTTCAATCTGTTCGTGT
TCTTCGAAATCCTGCTGATTGCTTCGTACGCGCTGCTGATCCACGGCGCAGGGCGCGACCGCATCGGCGCGGGACTGCAC
TACGTGTTGCTGAACCTTGTGGCATCATCGTTCTTTCTCGTCGCCATCGGCATGCTGTACGGGCTGACCGGAACACTAAA
CCTCGCGCATATAGGCTTGCGTTTGCAGGGCCTGCCGGCCTCAGATTTGCCGCTGGCGGTGGCCGCGGGTGTCATGCTGA
TGCTGGTATTCGGACTCAAGGCTGCCTTCTTTCCACTGTACTTCTGGCTGCCGCGTGCGTATGCGCAGACCGCGGGGTCG
GTGGCGGCGCTGTTCGCGGTGATGACAAAAGTCGGCCTGTACGCGATCCTGCGCTGTGACGCACTGGTCTTCGGCGCCGG
GCAAGGACTGCTCGCGCCATTCATGCAGACATGGTTGCCGGTGCTGGCGCTGGCGACGCTGGCAGTGGGCGCCATCGGCG
CACTGGCAGCCACCAGCCTGCGTGCGCTGACCAGCTACCTTGTGCTCGCCTCCGTTGGACTGCTCGGCATTGCCGTCGCC
ATGCAGACGCCCGCCGCCTGGGCCGCTGCCCTGTACTACCTGCTTAGCACGACACTATGCACGGCGGCGCTGTTCCTGCT
GGCTGACGCGCTGGAGCCCCCGCATGCGGACGGGCAGGTGTCGATGGTGGCCGGCACGCGGCTCGCTGGTCTGCTTTACC
TGACCGGCGCGGTGGTGGCGGTGGGCCTGCCGCCGCTGTCGGGCTTCCTCGGCAAGGTCATGATCCTGCGCGCCGTACCG
ACCGACAGTGCCATCGTCTACTGGCCTGCCATCCTGGCATCCGGACTGGTGTTGCTGGTGGCTGCGTCGCGCACAGGCAC
GCGCCTGCTGTGGCGCCTGCCGCACGATGGCAGCCACCCGTCGCAGGATGCCGCCGTGCCAGCGCGCGTCCGCTCACGCC
CCGAAGGCCGCAAGCTTGCCTGCAGCGCGTTCCTGCTGGCCTGCAACGTCATGCTGACGGCATTTGCCGCGCCGGTAAGC
GCCTATGTCGGACAGGCAGCCGTGCAACTGCAGGATCGCACTGCTTACGTTCGCGCCGTGCTGCCAGCCGGCGTTCCGGA
AACGGCGGAGAGGTAG

Upstream 100 bases:

>100_bases
CATTCGCGGTGGTGCTGTCGCTGCGCTCGCTGGGCCTGACAGGCAGCGACCATGTCGATGCGGCCGGACAAAACGATGCG
CAAGCGACGGAGGGCCGCCC

Downstream 100 bases:

>100_bases
GTCAATGCTCGCGCGCCAGTTCCCGCATCCATGGCTGAGCCTGATCCTGCTGCTGATGTGGCTGATGCTGTCCAACAGCG
TGGCGCCGGCACACTGGCTG

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MTHVLLLPILVPMFAGALLTAMPMHWLRAQRVVCMAATLLLVPVAGLLVDQAAGGSIAVYALGNWQAPFGIVLQLDRLGA
MMLGLTALLAPAALAAAGPELVRAGRHFHALFQFQLMGLNGAFLAGDLFNLFVFFEILLIASYALLIHGAGRDRIGAGLH
YVLLNLVASSFFLVAIGMLYGLTGTLNLAHIGLRLQGLPASDLPLAVAAGVMLMLVFGLKAAFFPLYFWLPRAYAQTAGS
VAALFAVMTKVGLYAILRCDALVFGAGQGLLAPFMQTWLPVLALATLAVGAIGALAATSLRALTSYLVLASVGLLGIAVA
MQTPAAWAAALYYLLSTTLCTAALFLLADALEPPHADGQVSMVAGTRLAGLLYLTGAVVAVGLPPLSGFLGKVMILRAVP
TDSAIVYWPAILASGLVLLVAASRTGTRLLWRLPHDGSHPSQDAAVPARVRSRPEGRKLACSAFLLACNVMLTAFAAPVS
AYVGQAAVQLQDRTAYVRAVLPAGVPETAER

Sequences:

>Translated_511_residues
MTHVLLLPILVPMFAGALLTAMPMHWLRAQRVVCMAATLLLVPVAGLLVDQAAGGSIAVYALGNWQAPFGIVLQLDRLGA
MMLGLTALLAPAALAAAGPELVRAGRHFHALFQFQLMGLNGAFLAGDLFNLFVFFEILLIASYALLIHGAGRDRIGAGLH
YVLLNLVASSFFLVAIGMLYGLTGTLNLAHIGLRLQGLPASDLPLAVAAGVMLMLVFGLKAAFFPLYFWLPRAYAQTAGS
VAALFAVMTKVGLYAILRCDALVFGAGQGLLAPFMQTWLPVLALATLAVGAIGALAATSLRALTSYLVLASVGLLGIAVA
MQTPAAWAAALYYLLSTTLCTAALFLLADALEPPHADGQVSMVAGTRLAGLLYLTGAVVAVGLPPLSGFLGKVMILRAVP
TDSAIVYWPAILASGLVLLVAASRTGTRLLWRLPHDGSHPSQDAAVPARVRSRPEGRKLACSAFLLACNVMLTAFAAPVS
AYVGQAAVQLQDRTAYVRAVLPAGVPETAER
>Mature_510_residues
THVLLLPILVPMFAGALLTAMPMHWLRAQRVVCMAATLLLVPVAGLLVDQAAGGSIAVYALGNWQAPFGIVLQLDRLGAM
MLGLTALLAPAALAAAGPELVRAGRHFHALFQFQLMGLNGAFLAGDLFNLFVFFEILLIASYALLIHGAGRDRIGAGLHY
VLLNLVASSFFLVAIGMLYGLTGTLNLAHIGLRLQGLPASDLPLAVAAGVMLMLVFGLKAAFFPLYFWLPRAYAQTAGSV
AALFAVMTKVGLYAILRCDALVFGAGQGLLAPFMQTWLPVLALATLAVGAIGALAATSLRALTSYLVLASVGLLGIAVAM
QTPAAWAAALYYLLSTTLCTAALFLLADALEPPHADGQVSMVAGTRLAGLLYLTGAVVAVGLPPLSGFLGKVMILRAVPT
DSAIVYWPAILASGLVLLVAASRTGTRLLWRLPHDGSHPSQDAAVPARVRSRPEGRKLACSAFLLACNVMLTAFAAPVSA
YVGQAAVQLQDRTAYVRAVLPAGVPETAER

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=339, Percent_Identity=26.8436578171091, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788613, Length=452, Percent_Identity=28.0973451327434, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788827, Length=293, Percent_Identity=29.3515358361775, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 53335; Mature: 53204

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVLLLPILVPMFAGALLTAMPMHWLRAQRVVCMAATLLLVPVAGLLVDQAAGGSIAVY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ALGNWQAPFGIVLQLDRLGAMMLGLTALLAPAALAAAGPELVRAGRHFHALFQFQLMGLN
EECCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHEECCC
GAFLAGDLFNLFVFFEILLIASYALLIHGAGRDRIGAGLHYVLLNLVASSFFLVAIGMLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTGTLNLAHIGLRLQGLPASDLPLAVAAGVMLMLVFGLKAAFFPLYFWLPRAYAQTAGS
CCCCCCCCEEECEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAALFAVMTKVGLYAILRCDALVFGAGQGLLAPFMQTWLPVLALATLAVGAIGALAATSL
HHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALTSYLVLASVGLLGIAVAMQTPAAWAAALYYLLSTTLCTAALFLLADALEPPHADGQV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
SMVAGTRLAGLLYLTGAVVAVGLPPLSGFLGKVMILRAVPTDSAIVYWPAILASGLVLLV
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHEEE
AASRTGTRLLWRLPHDGSHPSQDAAVPARVRSRPEGRKLACSAFLLACNVMLTAFAAPVS
ECCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYVGQAAVQLQDRTAYVRAVLPAGVPETAER
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
THVLLLPILVPMFAGALLTAMPMHWLRAQRVVCMAATLLLVPVAGLLVDQAAGGSIAVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
ALGNWQAPFGIVLQLDRLGAMMLGLTALLAPAALAAAGPELVRAGRHFHALFQFQLMGLN
EECCCCCCHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHEECCC
GAFLAGDLFNLFVFFEILLIASYALLIHGAGRDRIGAGLHYVLLNLVASSFFLVAIGMLY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLTGTLNLAHIGLRLQGLPASDLPLAVAAGVMLMLVFGLKAAFFPLYFWLPRAYAQTAGS
CCCCCCCCEEECEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAALFAVMTKVGLYAILRCDALVFGAGQGLLAPFMQTWLPVLALATLAVGAIGALAATSL
HHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RALTSYLVLASVGLLGIAVAMQTPAAWAAALYYLLSTTLCTAALFLLADALEPPHADGQV
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
SMVAGTRLAGLLYLTGAVVAVGLPPLSGFLGKVMILRAVPTDSAIVYWPAILASGLVLLV
EEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCHHHHHHHHHCCHHEEE
AASRTGTRLLWRLPHDGSHPSQDAAVPARVRSRPEGRKLACSAFLLACNVMLTAFAAPVS
ECCCCCCEEEEECCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYVGQAAVQLQDRTAYVRAVLPAGVPETAER
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]