Click here to switch to the map view.

The map label for this gene is ygfZ [C]

Identifier: 73541126

GI number: 73541126

Start: 1547302

End: 1548423

Strand: Reverse

Name: ygfZ [C]

Synonym: Reut_A1432

Alternate gene names: 73541126

Gene position: 1548423-1547302 (Counterclockwise)

Preceding gene: 73541134

Following gene: 73541125

Centisome position: 40.68

GC content: 68.27

Gene sequence:

>1122_bases
ATGCCGGCAGCCTCATATAATACCGGGAGCCTTGCGCGGCCACGACTTTGCCGGTCGGTGAACCGCGGCGCGCACCAGTC
CAATGCACCCAATTGCCTGATCGGAATGCCTGAAATGAATGCCCCAGCCAACAACCTCTCCGCCAGCTTTGACGCCTTGC
AGAATCGTGGCGTTGTCTGCGCACCGGCCGGGCTCGGATGGATCCGCGTGGCGGGCGATGATGCCGCTGCCTTCCTGCAC
ACGCAGCTGACCAACGCGGTCGAAGACCTCGGCCCCGGCGCTGCCCGCCTGGCCGGCTATTGCTCGCCCAAGGGCCGCCT
GCAGGCCAGCTTCCTGATGTGGCGCGATGCGGACGGCATCGTGCTGCAACTGTCAGACGACATCCAGCCAGCGCTGCAGA
AGCGCCTGAGCATGTTCGTCCTGCGCGCCAAGGCCAAGCTCAGCGACATGTCTCCTGTCGTGGCCGTGGTGGGCGCGGCG
GGTCCGCAAGCCGCCCAAGCACTGGCCAAGGCCGGACTGCCCGCGCCCGACGCAGTGTTCGGCACGGCCAGTGTTGAAAG
CGCAACCGTTATCCGCCTGCCGGACGCTGCTGGCCAGCCTCGCTGGCAAGCCGTGTTGCCGGCCGAACGCGCCGGAGAAT
TCCGTGCCGCGCTGAGCGGGGAATTGGCGGACGCCGACTCCGCGTTCTGGGACTGGCTCGATGTCCAGTCCGGCATCCCG
CGCATCGTCACCGCGACGCAGGAACAGTTCGTGCCGCAGATGATCAACTTCGAACTGGTCGGCGGCGTCAATTTCCGCAA
GGGCTGCTACCCGGGCCAGGAAATCGTGGCGCGCAGCCAGTACCGCGGCACGCTCAAGCGCCGCATGTGGCTGGTGCAGG
GCGACGGTGCCGTGCCGGCGCCCGCTACGGAGATCTTCCGCCCGGAAGATCCGCAGCAGCCGTGCGGCATGGTCGTGAAC
GCCGCCCCCGCCCCGCAAGGCGGCTGGGCGGGCTTGGCCGAACTGAAGATCGATGCGGCCGCGTCGGCCCTGCATCTGGG
CAGCGCCGAAGGCGCTGCGCTGACGGTGGGCGAACTCCCCTACGAAGTACCGCTGCCGGAAGCCACGCAGGCCGCCGGCT
GA

Upstream 100 bases:

>100_bases
TCCCAGGCGTAGAAAAAGACGTGTCATGAAATCCGTTCGGTCGCGGCAGCGTCCGGACTGCGGGTGATGTCCCAGCCGCG
CGCTGCCGGCGCCGGCTGAA

Downstream 100 bases:

>100_bases
CCCCAGGACGCTGCCATGAGCGATCACGTCTTCGTCTATTTCCGCGTCCCCGAAGCGCTGGCAACCGAAGCGCTACCGCA
CTGGCATCGCTGGATGGAGA

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPAASYNTGSLARPRLCRSVNRGAHQSNAPNCLIGMPEMNAPANNLSASFDALQNRGVVCAPAGLGWIRVAGDDAAAFLH
TQLTNAVEDLGPGAARLAGYCSPKGRLQASFLMWRDADGIVLQLSDDIQPALQKRLSMFVLRAKAKLSDMSPVVAVVGAA
GPQAAQALAKAGLPAPDAVFGTASVESATVIRLPDAAGQPRWQAVLPAERAGEFRAALSGELADADSAFWDWLDVQSGIP
RIVTATQEQFVPQMINFELVGGVNFRKGCYPGQEIVARSQYRGTLKRRMWLVQGDGAVPAPATEIFRPEDPQQPCGMVVN
AAPAPQGGWAGLAELKIDAAASALHLGSAEGAALTVGELPYEVPLPEATQAAG

Sequences:

>Translated_373_residues
MPAASYNTGSLARPRLCRSVNRGAHQSNAPNCLIGMPEMNAPANNLSASFDALQNRGVVCAPAGLGWIRVAGDDAAAFLH
TQLTNAVEDLGPGAARLAGYCSPKGRLQASFLMWRDADGIVLQLSDDIQPALQKRLSMFVLRAKAKLSDMSPVVAVVGAA
GPQAAQALAKAGLPAPDAVFGTASVESATVIRLPDAAGQPRWQAVLPAERAGEFRAALSGELADADSAFWDWLDVQSGIP
RIVTATQEQFVPQMINFELVGGVNFRKGCYPGQEIVARSQYRGTLKRRMWLVQGDGAVPAPATEIFRPEDPQQPCGMVVN
AAPAPQGGWAGLAELKIDAAASALHLGSAEGAALTVGELPYEVPLPEATQAAG
>Mature_372_residues
PAASYNTGSLARPRLCRSVNRGAHQSNAPNCLIGMPEMNAPANNLSASFDALQNRGVVCAPAGLGWIRVAGDDAAAFLHT
QLTNAVEDLGPGAARLAGYCSPKGRLQASFLMWRDADGIVLQLSDDIQPALQKRLSMFVLRAKAKLSDMSPVVAVVGAAG
PQAAQALAKAGLPAPDAVFGTASVESATVIRLPDAAGQPRWQAVLPAERAGEFRAALSGELADADSAFWDWLDVQSGIPR
IVTATQEQFVPQMINFELVGGVNFRKGCYPGQEIVARSQYRGTLKRRMWLVQGDGAVPAPATEIFRPEDPQQPCGMVVNA
APAPQGGWAGLAELKIDAAASALHLGSAEGAALTVGELPYEVPLPEATQAAG

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=296, Percent_Identity=25.6756756756757, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 38995; Mature: 38864

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAASYNTGSLARPRLCRSVNRGAHQSNAPNCLIGMPEMNAPANNLSASFDALQNRGVVC
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEE
APAGLGWIRVAGDDAAAFLHTQLTNAVEDLGPGAARLAGYCSPKGRLQASFLMWRDADGI
ECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEECCCCE
VLQLSDDIQPALQKRLSMFVLRAKAKLSDMSPVVAVVGAAGPQAAQALAKAGLPAPDAVF
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEE
GTASVESATVIRLPDAAGQPRWQAVLPAERAGEFRAALSGELADADSAFWDWLDVQSGIP
ECCCCCCEEEEECCCCCCCCCEEEECCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RIVTATQEQFVPQMINFELVGGVNFRKGCYPGQEIVARSQYRGTLKRRMWLVQGDGAVPA
CEEECCHHHHHHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCC
PATEIFRPEDPQQPCGMVVNAAPAPQGGWAGLAELKIDAAASALHLGSAEGAALTVGELP
CHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEHHHHHHHCCCCCCCCEEEECCCC
YEVPLPEATQAAG
CCCCCCCHHCCCC
>Mature Secondary Structure 
PAASYNTGSLARPRLCRSVNRGAHQSNAPNCLIGMPEMNAPANNLSASFDALQNRGVVC
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEE
APAGLGWIRVAGDDAAAFLHTQLTNAVEDLGPGAARLAGYCSPKGRLQASFLMWRDADGI
ECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEEEEEEECCCCE
VLQLSDDIQPALQKRLSMFVLRAKAKLSDMSPVVAVVGAAGPQAAQALAKAGLPAPDAVF
EEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCEE
GTASVESATVIRLPDAAGQPRWQAVLPAERAGEFRAALSGELADADSAFWDWLDVQSGIP
ECCCCCCEEEEECCCCCCCCCEEEECCHHHCCHHHHHHCCCCCCCCHHHHHHHHHHCCCC
RIVTATQEQFVPQMINFELVGGVNFRKGCYPGQEIVARSQYRGTLKRRMWLVQGDGAVPA
CEEECCHHHHHHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCC
PATEIFRPEDPQQPCGMVVNAAPAPQGGWAGLAELKIDAAASALHLGSAEGAALTVGELP
CHHHCCCCCCCCCCCCEEEECCCCCCCCCCCHHHEEEHHHHHHHCCCCCCCCEEEECCCC
YEVPLPEATQAAG
CCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA