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The map label for this gene is gatA [H]

Identifier: 73541082

GI number: 73541082

Start: 1500037

End: 1501392

Strand: Reverse

Name: gatA [H]

Synonym: Reut_A1388

Alternate gene names: 73541082

Gene position: 1501392-1500037 (Counterclockwise)

Preceding gene: 73541085

Following gene: 73541081

Centisome position: 39.44

GC content: 68.66

Gene sequence:

>1356_bases
ATGCTTCCTGACCTCAACACACTGCATGCCCGTCTGCGCGAAGGCGCCATAAGCCGCGTCGAACTGATCGAAGCCGCTGC
CGATGCAGCCTCCCAGCCGCGCGCCCAGGCAGTCTTCCTGCATAGCACGTTCGATACCGCCCTCCAGACCGCCCGCGCCG
CCGATGCGGCGGGCCGTGCCGGCAAAGCGCTGCACCCTCTGGCCGGCTTGCCGGTTTCGGTCAAGGATCTCTTCGATGTG
GCCGGCGAGGTCACGCGCGCAGCCTCGGCGGTACGGCACGACGCTCCCCCGGCGACGGCCGACGCCACCGTGGTCGCACG
CCTGCGCCACGCCGGTGCGGCCCTGGTCGGGCGCACCAACATGACCGAGTTCGCCTTCTCGGGCGTGGGCATCAATCCCC
ATTTCGGCACGCCGGTGAACCCGGCAAGCGCCGATGGTATCGCCCGCATCCCCGGAGGATCGTCGTCGGGCGCGGCCGTT
TCGGTGGCACTCGGGCTGGCGGTCGCGGCGCTCGGCAGCGACACAGGCGGATCGATCCGAATTCCCGCAGCGCTGTGCGG
CCTGACCGGGTTCAAACCGACCACGCGCCGCGTGCCGCTGACCGGCGCCTTTCCATTGTCCTACACGCTGGACACCGCCT
GTGCGATGGCTCGCACGGTCAATGACTGCCTGCTGGTGGACAGTGTAATTGCCGACAACGCGCTGGTGCCAAGCGCTCCC
GCCGCCGCGGCGCTTCGCCTGGCCATCCCGCGCCAGGTACTGCTGGACGACCTCGACCCCGTGGTCGCGCGCGCGTTCGA
CCGCGCGCTGGGCCGATTGTCGGCCGCCGGCGTGCAGCTGGAGCACACCGACCTGCCTGAACTCGCCGAACTCCCGGGCC
TGAACGCCGCAGGCGGCTTCAGCGCGGCCGAGGCCTTTTCCATCCATCGCCACACACTCGCCACGCGGCGCAACATGTAC
GACCCGCGCGTTGCCCTGCGCATTGACCGCGGAGCAGCCATGGGTGCAGCGGACTACGTCGATCTGGCCCGCGCGCGCAT
CGACTGGATTTCACGCGTGGAAGCCCGCCTCGCGCGCTTTGATGCGGTCATCTGCCCGACCGTGCCGATGGTCGCTCCCG
CCATTGAACCGCTGCGCGCAGACGACGATTTGTTCCTCCGCACCAACGCGCTGCTGCTTCGCAATACCTCGGCCTTCAAC
TTCCTCGACGGCGGCTCGATCTCGCTGCCCTGCCATGCGCCCGACGAACTGCCCGTGGGGTTGATGCTGTCCCATGGCCC
CATGCGCGATGCGCAATTGATCGGCACCGCCCTTGCATTGGAAAGCATCGTGCAGCCTTCCCTGCGCGACGAATAA

Upstream 100 bases:

>100_bases
ATCGGCAAATTATGAGCATGAGTGACTGACAAGAACAGGCAGGGCTCGGACGGCGGCACGCCTGGAGCCAGCGCGCACCC
TGCCCGCCTCCACGCCACCG

Downstream 100 bases:

>100_bases
GCCAGCCCGGCGCCCGATCGCCGCGGGCGCCGGCGCGATCCGAAACCGCATGAATACGACCCGCCCCCACCCTTCCCTTG
CGGCCGATGACTGGACTGCA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLPDLNTLHARLREGAISRVELIEAAADAASQPRAQAVFLHSTFDTALQTARAADAAGRAGKALHPLAGLPVSVKDLFDV
AGEVTRAASAVRHDAPPATADATVVARLRHAGAALVGRTNMTEFAFSGVGINPHFGTPVNPASADGIARIPGGSSSGAAV
SVALGLAVAALGSDTGGSIRIPAALCGLTGFKPTTRRVPLTGAFPLSYTLDTACAMARTVNDCLLVDSVIADNALVPSAP
AAAALRLAIPRQVLLDDLDPVVARAFDRALGRLSAAGVQLEHTDLPELAELPGLNAAGGFSAAEAFSIHRHTLATRRNMY
DPRVALRIDRGAAMGAADYVDLARARIDWISRVEARLARFDAVICPTVPMVAPAIEPLRADDDLFLRTNALLLRNTSAFN
FLDGGSISLPCHAPDELPVGLMLSHGPMRDAQLIGTALALESIVQPSLRDE

Sequences:

>Translated_451_residues
MLPDLNTLHARLREGAISRVELIEAAADAASQPRAQAVFLHSTFDTALQTARAADAAGRAGKALHPLAGLPVSVKDLFDV
AGEVTRAASAVRHDAPPATADATVVARLRHAGAALVGRTNMTEFAFSGVGINPHFGTPVNPASADGIARIPGGSSSGAAV
SVALGLAVAALGSDTGGSIRIPAALCGLTGFKPTTRRVPLTGAFPLSYTLDTACAMARTVNDCLLVDSVIADNALVPSAP
AAAALRLAIPRQVLLDDLDPVVARAFDRALGRLSAAGVQLEHTDLPELAELPGLNAAGGFSAAEAFSIHRHTLATRRNMY
DPRVALRIDRGAAMGAADYVDLARARIDWISRVEARLARFDAVICPTVPMVAPAIEPLRADDDLFLRTNALLLRNTSAFN
FLDGGSISLPCHAPDELPVGLMLSHGPMRDAQLIGTALALESIVQPSLRDE
>Mature_451_residues
MLPDLNTLHARLREGAISRVELIEAAADAASQPRAQAVFLHSTFDTALQTARAADAAGRAGKALHPLAGLPVSVKDLFDV
AGEVTRAASAVRHDAPPATADATVVARLRHAGAALVGRTNMTEFAFSGVGINPHFGTPVNPASADGIARIPGGSSSGAAV
SVALGLAVAALGSDTGGSIRIPAALCGLTGFKPTTRRVPLTGAFPLSYTLDTACAMARTVNDCLLVDSVIADNALVPSAP
AAAALRLAIPRQVLLDDLDPVVARAFDRALGRLSAAGVQLEHTDLPELAELPGLNAAGGFSAAEAFSIHRHTLATRRNMY
DPRVALRIDRGAAMGAADYVDLARARIDWISRVEARLARFDAVICPTVPMVAPAIEPLRADDDLFLRTNALLLRNTSAFN
FLDGGSISLPCHAPDELPVGLMLSHGPMRDAQLIGTALALESIVQPSLRDE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=27.3622047244095, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI195972892, Length=205, Percent_Identity=32.6829268292683, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI166795287, Length=136, Percent_Identity=35.2941176470588, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=462, Percent_Identity=29.6536796536797, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=200, Percent_Identity=33, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556264, Length=134, Percent_Identity=39.5522388059701, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=31.1557788944724, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=440, Percent_Identity=26.5909090909091, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=440, Percent_Identity=26.5909090909091, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=199, Percent_Identity=30.6532663316583, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=29.7979797979798, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=155, Percent_Identity=38.7096774193548, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648113, Length=305, Percent_Identity=31.4754098360656, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550774, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24648435, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648437, Length=244, Percent_Identity=31.1475409836066, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24648441, Length=185, Percent_Identity=32.972972972973, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648439, Length=185, Percent_Identity=32.972972972973, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24644968, Length=213, Percent_Identity=31.924882629108, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356731, Length=259, Percent_Identity=28.957528957529, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24652985, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922090, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652981, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652983, Length=414, Percent_Identity=24.1545893719807, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161078093, Length=218, Percent_Identity=32.1100917431193, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46844; Mature: 46844

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPDLNTLHARLREGAISRVELIEAAADAASQPRAQAVFLHSTFDTALQTARAADAAGRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
GKALHPLAGLPVSVKDLFDVAGEVTRAASAVRHDAPPATADATVVARLRHAGAALVGRTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEECCCC
MTEFAFSGVGINPHFGTPVNPASADGIARIPGGSSSGAAVSVALGLAVAALGSDTGGSIR
CHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
IPAALCGLTGFKPTTRRVPLTGAFPLSYTLDTACAMARTVNDCLLVDSVIADNALVPSAP
CCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AAAALRLAIPRQVLLDDLDPVVARAFDRALGRLSAAGVQLEHTDLPELAELPGLNAAGGF
HHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHCCCCCCCCCC
SAAEAFSIHRHTLATRRNMYDPRVALRIDRGAAMGAADYVDLARARIDWISRVEARLARF
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DAVICPTVPMVAPAIEPLRADDDLFLRTNALLLRNTSAFNFLDGGSISLPCHAPDELPVG
CCHHCCCCHHHHHCCCCCCCCCCEEEEECEEEEECCCCCEEECCCCEEECCCCCCCCCEE
LMLSHGPMRDAQLIGTALALESIVQPSLRDE
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLPDLNTLHARLREGAISRVELIEAAADAASQPRAQAVFLHSTFDTALQTARAADAAGRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
GKALHPLAGLPVSVKDLFDVAGEVTRAASAVRHDAPPATADATVVARLRHAGAALVGRTN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEECCCC
MTEFAFSGVGINPHFGTPVNPASADGIARIPGGSSSGAAVSVALGLAVAALGSDTGGSIR
CHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCEE
IPAALCGLTGFKPTTRRVPLTGAFPLSYTLDTACAMARTVNDCLLVDSVIADNALVPSAP
CCHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AAAALRLAIPRQVLLDDLDPVVARAFDRALGRLSAAGVQLEHTDLPELAELPGLNAAGGF
HHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHCCCCCCCCCC
SAAEAFSIHRHTLATRRNMYDPRVALRIDRGAAMGAADYVDLARARIDWISRVEARLARF
CHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DAVICPTVPMVAPAIEPLRADDDLFLRTNALLLRNTSAFNFLDGGSISLPCHAPDELPVG
CCHHCCCCHHHHHCCCCCCCCCCEEEEECEEEEECCCCCEEECCCCEEECCCCCCCCCEE
LMLSHGPMRDAQLIGTALALESIVQPSLRDE
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA