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The map label for this gene is boxA [H]

Identifier: 73541017

GI number: 73541017

Start: 1417262

End: 1418515

Strand: Reverse

Name: boxA [H]

Synonym: Reut_A1323

Alternate gene names: 73541017

Gene position: 1418515-1417262 (Counterclockwise)

Preceding gene: 73541018

Following gene: 73541016

Centisome position: 37.27

GC content: 63.96

Gene sequence:

>1254_bases
ATGGGCGCCCCCGAAATGATCAAGCAGCACCTGATCGATCCGGAAATCTGCATTCGCTGCAATACCTGCGAAGACACGTG
TCCGATCGACGCGATCACCCATGACGAGCGCAACTATGTGGTGAAGGCCGACGTCTGTAACGCGTGCAACGCCTGCCTCT
CGCCATGCCCGACCGGCGCCATCGACAACTGGCGCACCATGCTCAAGGGCCAGGCCTATCCGATCGAAGAGCAACTGCTG
TGGGATGAACTGCCGGCCGAGATACCGCTGCCTGCAGCGGAACTCGATGCAGCCGGCACGGACGAAGCCCTACGCGCAGA
CACCGCCACAGCTGCCGCAGAAGGCGGTGTAACGGTACAAGCCGTGGAAACGAGCCGACACACGTCGGCGCGCGCGCCAT
GGTCGGCAGCCCATCCATATGTCAACCTGCACGGCGTCCGGGCGCCCATGGCGGCTACCGTGGCTGGCAACTACAGGCTG
ACCGCCGAGGATGCATCAAGCGACATCCACCACATCGTGCTGGACTTCGGCACGCATTTCTTCCCGGTGCTGGAAGGACA
GGCCATCGGCATCGTGCCGCCCGGCACAGACGCAGCCGGCAAGCCGCACTACATCCGCATGTACTCCGTTGCCAGCCCGC
GCGACGGCGAACGGCCTGGCTACAACAATCTTGCGCTGACCGTGAAACGCGTCGAGGAAGACCACGACGGCAATCCGGTG
CGCGGCGTGGCGTCGAACTTCCTGTGCGACCTGGCCAAGGGCGATACCGTGCAGGTGGTCGGGCCGTTCGGCACTACGTT
CCTAATGCCCAATCACCGGGAAGCCAGCATCATGATGATCTGCACCGGCACGGGCTCGGCTCCCATGCGCGCGATGACCG
AGCGCATGCGGCGCAACATGGAGCACTTCGGTGGGCGTCGCATGCTTTTCTTCGGTGCGCGCAATGCTCGGGAGTTGCCG
TACTTCGGCCCGCTGCTGAAGCTGCCGAAAGATTTCCTCGACATCCATTTCGCGTTCTCGCGCGACGCCGAGGTGCCACG
CCGCTATGTGCAGGACGCGATCCGCGAGGCGTCGGCCGGCGTGACGGCCCTGCTGTCCGATCCGCATGGCCATGTCTACA
TCTGCGGCCTGAAGGGGATGGAAGAAGGCGTGCTGGCAGCCTTCGGCGATGTCTGCGCCGCGTCAGGCATGTCGTGGCCG
GAACTGGAGGCCACCATGAAGGCCGAAGGCCGGTTGCATATCGAGACTTACTGA

Upstream 100 bases:

>100_bases
TTTCGAGTACGTGCGCTTCAACTGAATGACCGCCACCACAAGAAAGACAGGACTGTAGCCCCGGGGCCGAAGCCGGCCCC
GCCCCACATGGAGACATACC

Downstream 100 bases:

>100_bases
CCCCTCTTTGGCCTGGCTATGCCCGCCGGCCCGTGCGGTTCGCGGGCCGGTTTCCTTGCATCTCGCACTTCCCTTCTGAA
ATGACTCGCCTATAATGTTT

Product: benzoyl-CoA oxygenase, component A

Products: NA

Alternate protein names: Benzoyl-CoA 2,3-dioxygenase subunit A; Benzoyl-CoA dioxygenase reductase component [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MGAPEMIKQHLIDPEICIRCNTCEDTCPIDAITHDERNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYPIEEQLL
WDELPAEIPLPAAELDAAGTDEALRADTATAAAEGGVTVQAVETSRHTSARAPWSAAHPYVNLHGVRAPMAATVAGNYRL
TAEDASSDIHHIVLDFGTHFFPVLEGQAIGIVPPGTDAAGKPHYIRMYSVASPRDGERPGYNNLALTVKRVEEDHDGNPV
RGVASNFLCDLAKGDTVQVVGPFGTTFLMPNHREASIMMICTGTGSAPMRAMTERMRRNMEHFGGRRMLFFGARNARELP
YFGPLLKLPKDFLDIHFAFSRDAEVPRRYVQDAIREASAGVTALLSDPHGHVYICGLKGMEEGVLAAFGDVCAASGMSWP
ELEATMKAEGRLHIETY

Sequences:

>Translated_417_residues
MGAPEMIKQHLIDPEICIRCNTCEDTCPIDAITHDERNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYPIEEQLL
WDELPAEIPLPAAELDAAGTDEALRADTATAAAEGGVTVQAVETSRHTSARAPWSAAHPYVNLHGVRAPMAATVAGNYRL
TAEDASSDIHHIVLDFGTHFFPVLEGQAIGIVPPGTDAAGKPHYIRMYSVASPRDGERPGYNNLALTVKRVEEDHDGNPV
RGVASNFLCDLAKGDTVQVVGPFGTTFLMPNHREASIMMICTGTGSAPMRAMTERMRRNMEHFGGRRMLFFGARNARELP
YFGPLLKLPKDFLDIHFAFSRDAEVPRRYVQDAIREASAGVTALLSDPHGHVYICGLKGMEEGVLAAFGDVCAASGMSWP
ELEATMKAEGRLHIETY
>Mature_416_residues
GAPEMIKQHLIDPEICIRCNTCEDTCPIDAITHDERNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYPIEEQLLW
DELPAEIPLPAAELDAAGTDEALRADTATAAAEGGVTVQAVETSRHTSARAPWSAAHPYVNLHGVRAPMAATVAGNYRLT
AEDASSDIHHIVLDFGTHFFPVLEGQAIGIVPPGTDAAGKPHYIRMYSVASPRDGERPGYNNLALTVKRVEEDHDGNPVR
GVASNFLCDLAKGDTVQVVGPFGTTFLMPNHREASIMMICTGTGSAPMRAMTERMRRNMEHFGGRRMLFFGARNARELPY
FGPLLKLPKDFLDIHFAFSRDAEVPRRYVQDAIREASAGVTALLSDPHGHVYICGLKGMEEGVLAAFGDVCAASGMSWPE
LEATMKAEGRLHIETY

Specific function: The BoxA/BoxB complex catalyzes the aerobic reduction/oxygenation of the aromatic ring of benzoyl-CoA to form 2,3-dihydro-2,3-dihydroxybenzoyl-CoA. BoxA acts as a reductase that uses NADPH to reduce the oxygenase component BoxB. BoxAB does not act on NADH

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=223, Percent_Identity=25.1121076233184, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI24041029, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789123, Length=312, Percent_Identity=25.3205128205128, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321143, Length=194, Percent_Identity=29.8969072164948, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583543, Length=225, Percent_Identity=30.2222222222222, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78706876, Length=225, Percent_Identity=30.2222222222222, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI78706872, Length=225, Percent_Identity=30.2222222222222, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017634
- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012146
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00037 Fer4; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.21 [H]

Molecular weight: Translated: 45347; Mature: 45216

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAPEMIKQHLIDPEICIRCNTCEDTCPIDAITHDERNYVVKADVCNACNACLSPCPTGA
CCCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCC
IDNWRTMLKGQAYPIEEQLLWDELPAEIPLPAAELDAAGTDEALRADTATAAAEGGVTVQ
CHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHCCHHHHCCCCEEEE
AVETSRHTSARAPWSAAHPYVNLHGVRAPMAATVAGNYRLTAEDASSDIHHIVLDFGTHF
EEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCEEHEEHHCCCE
FPVLEGQAIGIVPPGTDAAGKPHYIRMYSVASPRDGERPGYNNLALTVKRVEEDHDGNPV
EEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCC
RGVASNFLCDLAKGDTVQVVGPFGTTFLMPNHREASIMMICTGTGSAPMRAMTERMRRNM
HHHHHHHHHECCCCCEEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
EHFGGRRMLFFGARNARELPYFGPLLKLPKDFLDIHFAFSRDAEVPRRYVQDAIREASAG
HHCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHEEEEEECCCCHHHHHHHHHHHHHHCCC
VTALLSDPHGHVYICGLKGMEEGVLAAFGDVCAASGMSWPELEATMKAEGRLHIETY
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHEECCCEEEEEEC
>Mature Secondary Structure 
GAPEMIKQHLIDPEICIRCNTCEDTCPIDAITHDERNYVVKADVCNACNACLSPCPTGA
CCHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHCCCCCCC
IDNWRTMLKGQAYPIEEQLLWDELPAEIPLPAAELDAAGTDEALRADTATAAAEGGVTVQ
CHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHHHCCHHHHCCCCEEEE
AVETSRHTSARAPWSAAHPYVNLHGVRAPMAATVAGNYRLTAEDASSDIHHIVLDFGTHF
EEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCEEEECCCCCCCCEEHEEHHCCCE
FPVLEGQAIGIVPPGTDAAGKPHYIRMYSVASPRDGERPGYNNLALTVKRVEEDHDGNPV
EEEECCCEEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCC
RGVASNFLCDLAKGDTVQVVGPFGTTFLMPNHREASIMMICTGTGSAPMRAMTERMRRNM
HHHHHHHHHECCCCCEEEEECCCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
EHFGGRRMLFFGARNARELPYFGPLLKLPKDFLDIHFAFSRDAEVPRRYVQDAIREASAG
HHCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHEEEEEECCCCHHHHHHHHHHHHHHCCC
VTALLSDPHGHVYICGLKGMEEGVLAAFGDVCAASGMSWPELEATMKAEGRLHIETY
EEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11222587; 12399500 [H]