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The map label for this gene is folD [H]

Identifier: 73540993

GI number: 73540993

Start: 1389537

End: 1390388

Strand: Reverse

Name: folD [H]

Synonym: Reut_A1299

Alternate gene names: 73540993

Gene position: 1390388-1389537 (Counterclockwise)

Preceding gene: 73540995

Following gene: 73540992

Centisome position: 36.53

GC content: 66.31

Gene sequence:

>852_bases
ATGTCTGCACAACTGATCGACGGCAACGCTCTTGCCAAACAAATCCGTTCCGAAGCCGCCGTGCGCGCCGCCCGCCTGAC
CGAACGCGGCCACCAGCCCGGACTGGCCGTGGTCCTGGTCGGCGAGGACCCTGCCAGCCAGGTCTATGTGCGCAACAAGG
TCAAGGCCTGCGAGGACAACGGCTTTCATTCGTCGCTGGACCGCTATCCGGCCGACCTGTCCGAGGCCGAACTGCTGGCC
CGCATCGATGCACTGAACAACGATCCGAAGATCCACGGCATCCTGGTGCAGCTGCCGCTGCCCAAGCACATCGACAGCCA
CAAGGTGCTCGAAGCCATCGCCCCGGAAAAGGACGTGGATGGCTTCCATGTGGCCAATGCCGGGGCGCTGATGACCGGCG
CCCCGCTGTTCCGCCCGTGCACGCCGTATGGCTGCATGAAGATGCTGGAATCGATCAGTTACCCGCTGCGCGGCGCGCGC
GCGGTGGTGGTCGGCGCCTCGAACATCGTCGGCAAGCCGATGGCCATGCTGCTGCTGCAGGCCGGCGCCACCGTGACCAT
CTGCAACAGCAAGACGCGCGATATCGGCGCGCATACGCGTGACGCCGACGTGATCGTCGCCGCCGTGGGCAAGCGCAACC
TGATCACTGCCGACATGGTCAAGCCCGGCGCGGTCGTCATCGACGTGGGCATGAACCGCGACGACAACGGCAAGCTGTGC
GGCGACGTGGACTTCGCCGGCGTGCGCGAGGTGGCTGGCTACATCACGCCGGTGCCTGGCGGCGTTGGCCCGATGACCAT
CACCATGCTGCTGATCAACACGCTGGAAGCCGCCGAGCGCGCCGCCGGCTAA

Upstream 100 bases:

>100_bases
ATGCCGGCCCGTGACCCCGTATAATTCGCCTTTAAGCTTTGCCGACCGGGCGCCGCCCCGCGACGGCGCCTTCACTCCGG
CCACAAACCCGCCTCTCCCC

Downstream 100 bases:

>100_bases
GCCTGCGCGCCGTTGCGGCACGTGCCGCAACGGCACTGGAAAAGCACCGGGGCTGCCCCAATCTGCAAGCCATTGCCTCT
TCAGGCCTGATAACCGAGAA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSAQLIDGNALAKQIRSEAAVRAARLTERGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFHSSLDRYPADLSEAELLA
RIDALNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESISYPLRGAR
AVVVGASNIVGKPMAMLLLQAGATVTICNSKTRDIGAHTRDADVIVAAVGKRNLITADMVKPGAVVIDVGMNRDDNGKLC
GDVDFAGVREVAGYITPVPGGVGPMTITMLLINTLEAAERAAG

Sequences:

>Translated_283_residues
MSAQLIDGNALAKQIRSEAAVRAARLTERGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFHSSLDRYPADLSEAELLA
RIDALNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESISYPLRGAR
AVVVGASNIVGKPMAMLLLQAGATVTICNSKTRDIGAHTRDADVIVAAVGKRNLITADMVKPGAVVIDVGMNRDDNGKLC
GDVDFAGVREVAGYITPVPGGVGPMTITMLLINTLEAAERAAG
>Mature_282_residues
SAQLIDGNALAKQIRSEAAVRAARLTERGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFHSSLDRYPADLSEAELLAR
IDALNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESISYPLRGARA
VVVGASNIVGKPMAMLLLQAGATVTICNSKTRDIGAHTRDADVIVAAVGKRNLITADMVKPGAVVIDVGMNRDDNGKLCG
DVDFAGVREVAGYITPVPGGVGPMTITMLLINTLEAAERAAG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=47.1186440677966, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=45.4237288135593, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.0689655172414, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=29.1479820627803, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=54.6762589928058, Blast_Score=285, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=41.0169491525424, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=47.3867595818815, Blast_Score=265, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=43, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=303, Percent_Identity=27.3927392739274, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.9795918367347, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.9795918367347, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=45.6747404844291, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=45.6747404844291, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=45.6747404844291, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=45.6747404844291, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29801; Mature: 29670

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQLIDGNALAKQIRSEAAVRAARLTERGHQPGLAVVLVGEDPASQVYVRNKVKACEDN
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEHHHHHHHHCCC
GFHSSLDRYPADLSEAELLARIDALNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESISYPLRGARAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDIGAHTRDADVIVAAVGKRNLITADMVKPGAVVIDVGMNRDDNGKLC
CCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCEE
GDVDFAGVREVAGYITPVPGGVGPMTITMLLINTLEAAERAAG
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAQLIDGNALAKQIRSEAAVRAARLTERGHQPGLAVVLVGEDPASQVYVRNKVKACEDN
CCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHEEHHHHHHHHCCC
GFHSSLDRYPADLSEAELLARIDALNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVD
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHVANAGALMTGAPLFRPCTPYGCMKMLESISYPLRGARAVVVGASNIVGKPMAMLLLQ
CEEEECCCCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHCCCHHHHHHHH
AGATVTICNSKTRDIGAHTRDADVIVAAVGKRNLITADMVKPGAVVIDVGMNRDDNGKLC
CCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCEEEEEECCCCCCCCCEE
GDVDFAGVREVAGYITPVPGGVGPMTITMLLINTLEAAERAAG
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA