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The map label for this gene is rhdA [H]

Identifier: 73540879

GI number: 73540879

Start: 1267531

End: 1268391

Strand: Direct

Name: rhdA [H]

Synonym: Reut_A1183

Alternate gene names: 73540879

Gene position: 1267531-1268391 (Clockwise)

Preceding gene: 73540877

Following gene: 73540880

Centisome position: 33.3

GC content: 58.3

Gene sequence:

>861_bases
ATGAACGTAAGGGTTCTCATATCACCGGCCGATCTGGCGGAAATGATGCGGACTGAGTCCGTCGTGCTCATCGATACGAG
AGACGCGGCGAGCTACGCCGAGGCTCACCTGCCAGGCGCGGTGAATATCCACGAGATATTTACCTACTTGGCAACAACGA
CACAAGAGGGCCTGGCCGCCCTGCGGGAAGCATTTGCCGAGGCGTTCGGCAAGGCCGGACTGTCGGGAAAGGAAGTGGCA
GTCGTCTACGAGCAATCGATGACGACTGGATTCGGTCAATCCTGCCGAGGATACGTACTCCTCCGATACCTTGGATATCC
ACGGGACAAGATCAAGGTGCTCCACGGTGGCTACCGTGCATGGCACGCGGCGGGCTTTCCCAGCTCAACCGACACCCCGA
TGCCCGCGCCTGCCAGGTTTCCGCTTGCTAGCACGCAGGCCAGCATCCTCGTCGACCTGGAAGAAATGAAGAAGACGGTC
GCGGACCCACACATTGTCAAACTCGACGTACGCGACGTCGACGAGTGGATAGCCGATAGCTCCTCGCCCTACGGCAAGGA
TTTTTGTCCTCGCAAAGGCCGAATTCCAAACTCTGTCTGGATCGAGTGGTACCGGATGATGAAGCCGACTCCGGCCGGCC
AGATGTTCAAGTCACCTGCGGAAATTCTCGCCGAGTGTGCTACCGTCGGAATTCGTCGTGAAACGCCCGTCGTCGTCTTC
TGCTTCAAAGGGGCGCGAGCTTCCAATAGCTTTGTCGCCCTGGAAGAAGCCGGGATAGCCGACGTCCGGCTCTATCTTGG
TTCGTGGAATGAGTGGTCGAGGGACCTCTCTTTGCCTATCGAAGAGGGCGTGCCCTATTAG

Upstream 100 bases:

>100_bases
TCAGAATGAACGGGACTATTCCAATCGTCCGAAAGCTTGCTGTGCTCCAGTGTCAATTGTCCCGTCAAAGCAAGCTTGGC
GCGATCAGATGAGGGTCGTC

Downstream 100 bases:

>100_bases
AAGCGCTCTGCGCGCCAATGGCCCGATGCCTGCAAGCTTCGACGTGCCACGGTGTCTCCCTGTCAGAGGAGGCGAAGTTG
TTCCAAAGGATACTGGTCGC

Product: rhodanese-like protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MNVRVLISPADLAEMMRTESVVLIDTRDAASYAEAHLPGAVNIHEIFTYLATTTQEGLAALREAFAEAFGKAGLSGKEVA
VVYEQSMTTGFGQSCRGYVLLRYLGYPRDKIKVLHGGYRAWHAAGFPSSTDTPMPAPARFPLASTQASILVDLEEMKKTV
ADPHIVKLDVRDVDEWIADSSSPYGKDFCPRKGRIPNSVWIEWYRMMKPTPAGQMFKSPAEILAECATVGIRRETPVVVF
CFKGARASNSFVALEEAGIADVRLYLGSWNEWSRDLSLPIEEGVPY

Sequences:

>Translated_286_residues
MNVRVLISPADLAEMMRTESVVLIDTRDAASYAEAHLPGAVNIHEIFTYLATTTQEGLAALREAFAEAFGKAGLSGKEVA
VVYEQSMTTGFGQSCRGYVLLRYLGYPRDKIKVLHGGYRAWHAAGFPSSTDTPMPAPARFPLASTQASILVDLEEMKKTV
ADPHIVKLDVRDVDEWIADSSSPYGKDFCPRKGRIPNSVWIEWYRMMKPTPAGQMFKSPAEILAECATVGIRRETPVVVF
CFKGARASNSFVALEEAGIADVRLYLGSWNEWSRDLSLPIEEGVPY
>Mature_286_residues
MNVRVLISPADLAEMMRTESVVLIDTRDAASYAEAHLPGAVNIHEIFTYLATTTQEGLAALREAFAEAFGKAGLSGKEVA
VVYEQSMTTGFGQSCRGYVLLRYLGYPRDKIKVLHGGYRAWHAAGFPSSTDTPMPAPARFPLASTQASILVDLEEMKKTV
ADPHIVKLDVRDVDEWIADSSSPYGKDFCPRKGRIPNSVWIEWYRMMKPTPAGQMFKSPAEILAECATVGIRRETPVVVF
CFKGARASNSFVALEEAGIADVRLYLGSWNEWSRDLSLPIEEGVPY

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=287, Percent_Identity=28.2229965156794, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI194473668, Length=283, Percent_Identity=25.7950530035336, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI61835204, Length=283, Percent_Identity=25.7950530035336, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI194473681, Length=283, Percent_Identity=25.7950530035336, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI87081967, Length=268, Percent_Identity=23.8805970149254, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71997283, Length=254, Percent_Identity=25.9842519685039, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=256, Percent_Identity=26.171875, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 31558; Mature: 31558

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRVLISPADLAEMMRTESVVLIDTRDAASYAEAHLPGAVNIHEIFTYLATTTQEGLAA
CCEEEEECHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LREAFAEAFGKAGLSGKEVAVVYEQSMTTGFGQSCRGYVLLRYLGYPRDKIKVLHGGYRA
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHEEEEECCCHH
WHAAGFPSSTDTPMPAPARFPLASTQASILVDLEEMKKTVADPHIVKLDVRDVDEWIADS
EECCCCCCCCCCCCCCCCCCCCCCCCHHEEEEHHHHHHHHCCCCEEEEEHHHHHHHHCCC
SSPYGKDFCPRKGRIPNSVWIEWYRMMKPTPAGQMFKSPAEILAECATVGIRRETPVVVF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCCCEEEE
CFKGARASNSFVALEEAGIADVRLYLGSWNEWSRDLSLPIEEGVPY
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCHHCCCCC
>Mature Secondary Structure
MNVRVLISPADLAEMMRTESVVLIDTRDAASYAEAHLPGAVNIHEIFTYLATTTQEGLAA
CCEEEEECHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LREAFAEAFGKAGLSGKEVAVVYEQSMTTGFGQSCRGYVLLRYLGYPRDKIKVLHGGYRA
HHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHEEEEECCCHH
WHAAGFPSSTDTPMPAPARFPLASTQASILVDLEEMKKTVADPHIVKLDVRDVDEWIADS
EECCCCCCCCCCCCCCCCCCCCCCCCHHEEEEHHHHHHHHCCCCEEEEEHHHHHHHHCCC
SSPYGKDFCPRKGRIPNSVWIEWYRMMKPTPAGQMFKSPAEILAECATVGIRRETPVVVF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCCCEEEE
CFKGARASNSFVALEEAGIADVRLYLGSWNEWSRDLSLPIEEGVPY
EECCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]