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The map label for this gene is ppsA [H]

Identifier: 73540861

GI number: 73540861

Start: 1247489

End: 1249888

Strand: Direct

Name: ppsA [H]

Synonym: Reut_A1162

Alternate gene names: 73540861

Gene position: 1247489-1249888 (Clockwise)

Preceding gene: 73540860

Following gene: 73540862

Centisome position: 32.77

GC content: 57.88

Gene sequence:

>2400_bases
ATGGCTATCAATACCACGCAGTTAGTCTGGTTTGAGGAACTTCGGCGTGCCGACATCTGGCGCGTGGGCGGCAAGAACGC
CTCGCTGGGTGAACTGTTAGCCAGCCTGACAACTCACGGCATCCGTGTGCCCCCCGGGTTCGCCATTCCTGTCGACGCTT
ACTGGAATTTCCTTGAGGCAAACGGCTTGCGCGCAGTGCTGTCATCAACGCTTTCTGCCCTTGCCAGCGGAAAGCTCACT
CTGTCTGACGCAGGGCAGAAGATGCGCCGCACAATCCTCACCGGCGAGTGGCCCAGAGAGACCGCTCAGGCGATTGGGCA
GGCCTATGCCACCCTGAATAGGCGGACCGGCCAGTCCAACGTGGACGTCGCGGTACGTTCCAGTGCCACCGCCGAAGATC
TTCCGGAGGCGAGCTTTGCAGGGCAGCTGGAGTCCTATCTCAACATCAGAGGCGAGCACGCTTTGCTCGACGCCTGCCGC
CGTTGCTACGCGTCCCTTTTCACCGATCGGGCAATCAGCTACCGCCAAGCGAAGGGAATCAATCACCTGCAGATTGGGCT
GTCCGTCGGTGTGCAGCGCATGGTGCGCTCTGACCTGAGTGGCGCTGGTGTGATGTTCTCCATCGATACCGAAACCGGCT
TCGATAAAGTGGTTCTCATCAACGCGGCATGGGGGCTGGGTGAGAACGTCGTCCAGGGAGACGTCGATCCCGACGAGTAC
GAAGTGTTCAAGCCGTTGCTGTCAGATCTTTCCCTCACGCCTATCGTCGGGAAAAAGCTGGGCACCAAGGCCAGGAAGCT
CATATACACAAGCGATTCTACCTGCCCGACAAGGAATGTATCCACGACCTCAGGAGAACGCGAAGCTTTCGTGCTGAGCG
ACGAGGAAATCCTCACGTTATCGCGTTGGGCCTGCGCGATCGCCGATCACTACGGCCAGCCCATGGACATTGAATGGGCG
AAAGACGGCAAAACGGGAGAGCTATTCATTGTGCAGGCCCGCCCTGAAACCGTCCAGTCACGACGCGAATCAAGTGCGAT
CAAGACCTATCGTATCAAGAAAGCTGGCATGCCATTGGTTACCGGTGTAAGCATTGGTGATGCCATCGCGACCGGTCCTA
TTTGTGTCATCGAGAATTTGCGAGAGATGGGGCGCTTCGTCGATGGCGCTGTGCTCGTGACACGGACGACCGATCCCGAC
TGGGTACCTGTAATGCGGCGCGCGGCTGCAATTGTCACCGACCAGGGAGGGAGAACTTCCCATGCGGCGATAGTCAGCCG
GGAGCTAGGGCTTCCCGCAATTGTCGGGACGGGCAATGCAACTGGCGTGCTGCATGAGAAACAGGATGTGACGGTGTCTT
GTGCCGAAGGCCGGGAGGGCTTTGTTTACGAAGGTATCGCCGAGTACGAAGTCGAGGAGATCGATTTTGGCAGTATCCCG
ACGACCCGCACCAAAGTCATGCTGAATTTGGCAAATCCGGCGGCAGCATTTCGCTGGTGGCGAATGCCCGCCGACGGCGT
CGGGTTGGCGCGGATGGAGTTTGTAGTGAGCAACCATATCAAGATCCATCCAATGGCGCTGGCGCGCTACGACACTCTCA
AGGACGACACAGCAAAGCGAACCATCGCTGAGCTGACGGCTGCTTACGCCAGCAAGACAGAGTACTTTGTGAGCCGGCTC
GCGCGTGGATTGGGAAGGATTGCCGCCGTGCAATATCCGCGACCTGTGCTCGTTCGCATGAGCGATTTCAAGACGAATGA
ATATGCGCGGCTGATTGGTGGAGCGGAGTTCGAACCTACGGAGGAGAATCCCATGCTCGGATTCCGTGGGGCTTCAAGAT
ACTATTCACCCCTCTATCGGGATGGGTTTGCCTTGGAGTGCCAAGCGATCGTCCGCCTGCGCAAACAAATGGGCTTAAAC
AATGTCATTGTCATGATTCCCTTCTGCCGGTCCACCAAAGAAGCGGATCTGGTGCTCGAGGTAATGGCGGAGAATGGCAT
CGTGCGCCACAAGGATGGACTTGAGGTCTACGTGATGTGCGAAATTCCGTCCAATGTCATCCTGGCAAAGGCTTTCGCCC
AACGCTTCGACGGCTTCTCGATTGGCAGCAATGACCTGACACAGTTGGTGCTCGGCGTGGATCGCGACTCGGCCAATCTT
GCTGGCTTGTTCGACGAGGAAGACGACGCGGTCAAATGGATGATCCGGAACGTTATTACACAGGCGCATGAGGCCAACGC
CCACGTGGGCTTGTGCGGCCAAGCGCCGAGTGATCACCCTGATTTCGCCGGATTCCTTGTCCAATGCGGAATCGACTCGA
TCTCCGTGACCCCCGACAGCTTCCTGGCAGTTAAGCGCGCCGTTGCTGCGGCTGAAGCAGCTATCGCGTCAGTACGCTGA

Upstream 100 bases:

>100_bases
CCCGCGCCGACCTCACGGTCTGACGCTGATGACAGGTGCGGCGTGCAGCCTATGCCGATAAGGTCGTTGCGCGTCAGGTG
GAAGACTAGAGGAGCAGTTG

Downstream 100 bases:

>100_bases
GCAGCGTTGGACCGAGTGCGTGTCGCATCGCTGCTTTGATATTCGGCAACAACAGCCAACGCCTATTGGTTCTACATGCG
CCGCTCAGCAATTTTGAGCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MAINTTQLVWFEELRRADIWRVGGKNASLGELLASLTTHGIRVPPGFAIPVDAYWNFLEANGLRAVLSSTLSALASGKLT
LSDAGQKMRRTILTGEWPRETAQAIGQAYATLNRRTGQSNVDVAVRSSATAEDLPEASFAGQLESYLNIRGEHALLDACR
RCYASLFTDRAISYRQAKGINHLQIGLSVGVQRMVRSDLSGAGVMFSIDTETGFDKVVLINAAWGLGENVVQGDVDPDEY
EVFKPLLSDLSLTPIVGKKLGTKARKLIYTSDSTCPTRNVSTTSGEREAFVLSDEEILTLSRWACAIADHYGQPMDIEWA
KDGKTGELFIVQARPETVQSRRESSAIKTYRIKKAGMPLVTGVSIGDAIATGPICVIENLREMGRFVDGAVLVTRTTDPD
WVPVMRRAAAIVTDQGGRTSHAAIVSRELGLPAIVGTGNATGVLHEKQDVTVSCAEGREGFVYEGIAEYEVEEIDFGSIP
TTRTKVMLNLANPAAAFRWWRMPADGVGLARMEFVVSNHIKIHPMALARYDTLKDDTAKRTIAELTAAYASKTEYFVSRL
ARGLGRIAAVQYPRPVLVRMSDFKTNEYARLIGGAEFEPTEENPMLGFRGASRYYSPLYRDGFALECQAIVRLRKQMGLN
NVIVMIPFCRSTKEADLVLEVMAENGIVRHKDGLEVYVMCEIPSNVILAKAFAQRFDGFSIGSNDLTQLVLGVDRDSANL
AGLFDEEDDAVKWMIRNVITQAHEANAHVGLCGQAPSDHPDFAGFLVQCGIDSISVTPDSFLAVKRAVAAAEAAIASVR

Sequences:

>Translated_799_residues
MAINTTQLVWFEELRRADIWRVGGKNASLGELLASLTTHGIRVPPGFAIPVDAYWNFLEANGLRAVLSSTLSALASGKLT
LSDAGQKMRRTILTGEWPRETAQAIGQAYATLNRRTGQSNVDVAVRSSATAEDLPEASFAGQLESYLNIRGEHALLDACR
RCYASLFTDRAISYRQAKGINHLQIGLSVGVQRMVRSDLSGAGVMFSIDTETGFDKVVLINAAWGLGENVVQGDVDPDEY
EVFKPLLSDLSLTPIVGKKLGTKARKLIYTSDSTCPTRNVSTTSGEREAFVLSDEEILTLSRWACAIADHYGQPMDIEWA
KDGKTGELFIVQARPETVQSRRESSAIKTYRIKKAGMPLVTGVSIGDAIATGPICVIENLREMGRFVDGAVLVTRTTDPD
WVPVMRRAAAIVTDQGGRTSHAAIVSRELGLPAIVGTGNATGVLHEKQDVTVSCAEGREGFVYEGIAEYEVEEIDFGSIP
TTRTKVMLNLANPAAAFRWWRMPADGVGLARMEFVVSNHIKIHPMALARYDTLKDDTAKRTIAELTAAYASKTEYFVSRL
ARGLGRIAAVQYPRPVLVRMSDFKTNEYARLIGGAEFEPTEENPMLGFRGASRYYSPLYRDGFALECQAIVRLRKQMGLN
NVIVMIPFCRSTKEADLVLEVMAENGIVRHKDGLEVYVMCEIPSNVILAKAFAQRFDGFSIGSNDLTQLVLGVDRDSANL
AGLFDEEDDAVKWMIRNVITQAHEANAHVGLCGQAPSDHPDFAGFLVQCGIDSISVTPDSFLAVKRAVAAAEAAIASVR
>Mature_798_residues
AINTTQLVWFEELRRADIWRVGGKNASLGELLASLTTHGIRVPPGFAIPVDAYWNFLEANGLRAVLSSTLSALASGKLTL
SDAGQKMRRTILTGEWPRETAQAIGQAYATLNRRTGQSNVDVAVRSSATAEDLPEASFAGQLESYLNIRGEHALLDACRR
CYASLFTDRAISYRQAKGINHLQIGLSVGVQRMVRSDLSGAGVMFSIDTETGFDKVVLINAAWGLGENVVQGDVDPDEYE
VFKPLLSDLSLTPIVGKKLGTKARKLIYTSDSTCPTRNVSTTSGEREAFVLSDEEILTLSRWACAIADHYGQPMDIEWAK
DGKTGELFIVQARPETVQSRRESSAIKTYRIKKAGMPLVTGVSIGDAIATGPICVIENLREMGRFVDGAVLVTRTTDPDW
VPVMRRAAAIVTDQGGRTSHAAIVSRELGLPAIVGTGNATGVLHEKQDVTVSCAEGREGFVYEGIAEYEVEEIDFGSIPT
TRTKVMLNLANPAAAFRWWRMPADGVGLARMEFVVSNHIKIHPMALARYDTLKDDTAKRTIAELTAAYASKTEYFVSRLA
RGLGRIAAVQYPRPVLVRMSDFKTNEYARLIGGAEFEPTEENPMLGFRGASRYYSPLYRDGFALECQAIVRLRKQMGLNN
VIVMIPFCRSTKEADLVLEVMAENGIVRHKDGLEVYVMCEIPSNVILAKAFAQRFDGFSIGSNDLTQLVLGVDRDSANLA
GLFDEEDDAVKWMIRNVITQAHEANAHVGLCGQAPSDHPDFAGFLVQCGIDSISVTPDSFLAVKRAVAAAEAAIASVR

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=778, Percent_Identity=48.7146529562982, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=411, Percent_Identity=26.0340632603406, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789193, Length=417, Percent_Identity=26.1390887290168, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI48994992, Length=373, Percent_Identity=25.201072386059, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788726, Length=193, Percent_Identity=30.5699481865285, Blast_Score=86, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=235, Percent_Identity=31.063829787234, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87161; Mature: 87030

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINTTQLVWFEELRRADIWRVGGKNASLGELLASLTTHGIRVPPGFAIPVDAYWNFLEA
CCCCCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHC
NGLRAVLSSTLSALASGKLTLSDAGQKMRRTILTGEWPRETAQAIGQAYATLNRRTGQSN
CCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VDVAVRSSATAEDLPEASFAGQLESYLNIRGEHALLDACRRCYASLFTDRAISYRQAKGI
CCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NHLQIGLSVGVQRMVRSDLSGAGVMFSIDTETGFDKVVLINAAWGLGENVVQGDVDPDEY
CEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCHHH
EVFKPLLSDLSLTPIVGKKLGTKARKLIYTSDSTCPTRNVSTTSGEREAFVLSDEEILTL
HHHHHHHHCCCCCCHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHH
SRWACAIADHYGQPMDIEWAKDGKTGELFIVQARPETVQSRRESSAIKTYRIKKAGMPLV
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCHHEEEEHHCCCCEE
TGVSIGDAIATGPICVIENLREMGRFVDGAVLVTRTTDPDWVPVMRRAAAIVTDQGGRTS
ECCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHEEECCCCCCC
HAAIVSRELGLPAIVGTGNATGVLHEKQDVTVSCAEGREGFVYEGIAEYEVEEIDFGSIP
HHHHHHHCCCCCEEEECCCCCEEEECCCCCEEEECCCCCCCEECCHHHCCCEECCCCCCC
TTRTKVMLNLANPAAAFRWWRMPADGVGLARMEFVVSNHIKIHPMALARYDTLKDDTAKR
CCCEEEEEEECCCHHHHHEECCCCCCCCHHHHHHHHHCCEEEEEEEHHHHHCCCCHHHHH
TIAELTAAYASKTEYFVSRLARGLGRIAAVQYPRPVLVRMSDFKTNEYARLIGGAEFEPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCC
EENPMLGFRGASRYYSPLYRDGFALECQAIVRLRKQMGLNNVIVMIPFCRSTKEADLVLE
CCCCCEEECCCHHHHCHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
VMAENGIVRHKDGLEVYVMCEIPSNVILAKAFAQRFDGFSIGSNDLTQLVLGVDRDSANL
HHHHCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCE
AGLFDEEDDAVKWMIRNVITQAHEANAHVGLCGQAPSDHPDFAGFLVQCGIDSISVTPDS
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHCCCCCEECCCHH
FLAVKRAVAAAEAAIASVR
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AINTTQLVWFEELRRADIWRVGGKNASLGELLASLTTHGIRVPPGFAIPVDAYWNFLEA
CCCCHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCEECCCCCEEEHHHHHHHHHC
NGLRAVLSSTLSALASGKLTLSDAGQKMRRTILTGEWPRETAQAIGQAYATLNRRTGQSN
CCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VDVAVRSSATAEDLPEASFAGQLESYLNIRGEHALLDACRRCYASLFTDRAISYRQAKGI
CCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NHLQIGLSVGVQRMVRSDLSGAGVMFSIDTETGFDKVVLINAAWGLGENVVQGDVDPDEY
CEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEECCCCCHHH
EVFKPLLSDLSLTPIVGKKLGTKARKLIYTSDSTCPTRNVSTTSGEREAFVLSDEEILTL
HHHHHHHHCCCCCCHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHH
SRWACAIADHYGQPMDIEWAKDGKTGELFIVQARPETVQSRRESSAIKTYRIKKAGMPLV
HHHHHHHHHHCCCCCCEEECCCCCCCCEEEEECCCHHHHHHHHHCCHHEEEEHHCCCCEE
TGVSIGDAIATGPICVIENLREMGRFVDGAVLVTRTTDPDWVPVMRRAAAIVTDQGGRTS
ECCCCCCHHHCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHEEECCCCCCC
HAAIVSRELGLPAIVGTGNATGVLHEKQDVTVSCAEGREGFVYEGIAEYEVEEIDFGSIP
HHHHHHHCCCCCEEEECCCCCEEEECCCCCEEEECCCCCCCEECCHHHCCCEECCCCCCC
TTRTKVMLNLANPAAAFRWWRMPADGVGLARMEFVVSNHIKIHPMALARYDTLKDDTAKR
CCCEEEEEEECCCHHHHHEECCCCCCCCHHHHHHHHHCCEEEEEEEHHHHHCCCCHHHHH
TIAELTAAYASKTEYFVSRLARGLGRIAAVQYPRPVLVRMSDFKTNEYARLIGGAEFEPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHCCCCCCCC
EENPMLGFRGASRYYSPLYRDGFALECQAIVRLRKQMGLNNVIVMIPFCRSTKEADLVLE
CCCCCEEECCCHHHHCHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
VMAENGIVRHKDGLEVYVMCEIPSNVILAKAFAQRFDGFSIGSNDLTQLVLGVDRDSANL
HHHHCCEEEECCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHEECCCCCCCE
AGLFDEEDDAVKWMIRNVITQAHEANAHVGLCGQAPSDHPDFAGFLVQCGIDSISVTPDS
EECCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHCCCCCEECCCHH
FLAVKRAVAAAEAAIASVR
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]