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The map label for this gene is eno [H]

Identifier: 73540793

GI number: 73540793

Start: 1184857

End: 1186146

Strand: Direct

Name: eno [H]

Synonym: Reut_A1091

Alternate gene names: 73540793

Gene position: 1184857-1186146 (Clockwise)

Preceding gene: 73540792

Following gene: 73540794

Centisome position: 31.13

GC content: 63.57

Gene sequence:

>1290_bases
ATGAGTGCAATCGTAGATATCATCGGTCGCGAGGTTCTCGACTCGCGCGGCAACCCTACCGTCGAATGCGACGTGCTGCT
GGAGTCCGGCGTGATGGGCCGTGCGGCAGTGCCGTCGGGCGCATCGACCGGTTCGCGCGAAGCCATCGAGCTGCGTGACG
GCGACAAGAGCCGCTACCTGGGCAAGGGCGTGCTGAAGGCCGTCGAGCACATCAACACCGAGATCTCCGAAGCCATCATG
GGCCTGGACGCTTCCGAGCAGGCCTTCCTGGACCGCACCCTGATCGACCTGGACGGCACCGAGAACAAGAGCCGCCTGGG
CGCCAACGCCATGCTGGCCGTGTCGATGGCCGTGGCCAAAGCTGCCGCCGAGGAAGCCGGTCTGCCGCTGTACCGCTACT
TCGGCGGTTCGGGCGCCATGCAGATGCCGGTGCCGATGATGAACATCGTCAACGGGGGCGCGCACGCCAACAACAGCCTG
GACATCCAGGAATTCATGGTCATGCCGGTGTCGGCGACCAGCTTCCGTGAAGCGCTGCGTTGCGGCGCCGAAGTCTTCCA
CGCGCTGAAGAAGATTCTGGCCGACAAGGGCATGTCCACCGCCGTGGGCGACGAGGGCGGCTTCGCCCCGAACTTCTCGT
CGAACGAGGAGTGCCTGAACACCATCGTCCAGGCCGTCGAGAAGGCCGGCTATCGCATGGGTGAAGACGTGCTGCTGGCG
CTGGACTGCGCCGCGAGCGAGTTCTACCACGAAGCCGAAGACGTCTACGTGCTGGAAGGCGAAGGCCTGAAGCTGTCGTC
GACGCAATTCGCCGATTACCTGGCCAACCTGTGCGACAAGTTCCCGATCGTCTCGATCGAGGACGGCATGGCCGAAGGCG
ACTGGGACGGCTGGAAGACCCTGACCGACAAGCTCGGCAAGCGCGTGCAGCTGGTCGGCGACGACCTGTTCGTGACCAAC
ACGAAGATCCTGAAGGAAGGCATCGACAAGGGCATCGGCAACTCGATCCTGATCAAGATCAACCAGATCGGTACGCTGAC
CGAGACGTTTGCCGCCATCGAAATGGCCAAGCGCGCCGGCTACACCGCCGTGATCTCGCACCGTTCGGGCGAGACCGAGG
ACAGCACCATCGCCGACATCGCTGTCGGTACCAATGCCGGCCAGATCAAGACCGGCTCCCTGTCGCGTTCGGACCGCATC
TCGAAGTACAACCAGCTGCTGCGTATCGAGGAAGACCTCGGCGATATCGCCAGCTACCCGGGCAAGAGCGCGTTCTACAA
CCTGCGCTGA

Upstream 100 bases:

>100_bases
AGCTCCCGAAATGGACTTTCGGGGCGCGTGACATCGCCCTGACACACGAGCAGGTTGCAGTAATCAGCAGCAGCAAGAAT
CAACAGTCAGAGAGGAATTC

Downstream 100 bases:

>100_bases
GCCAAGGCAGGCGACTGACTCGCCTCTGACGGCGGCGGCACGGATTGCATCCGGCCGCCGCCGTTGTTGTATCCGGATTT
GTATCGAAATCTGGCCGGCA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1 [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMVMPVSATSFREALRCGAEVFHALKKILADKGMSTAVGDEGGFAPNFSSNEECLNTIVQAVEKAGYRMGEDVLLA
LDCAASEFYHEAEDVYVLEGEGLKLSSTQFADYLANLCDKFPIVSIEDGMAEGDWDGWKTLTDKLGKRVQLVGDDLFVTN
TKILKEGIDKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRI
SKYNQLLRIEEDLGDIASYPGKSAFYNLR

Sequences:

>Translated_429_residues
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIM
GLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSL
DIQEFMVMPVSATSFREALRCGAEVFHALKKILADKGMSTAVGDEGGFAPNFSSNEECLNTIVQAVEKAGYRMGEDVLLA
LDCAASEFYHEAEDVYVLEGEGLKLSSTQFADYLANLCDKFPIVSIEDGMAEGDWDGWKTLTDKLGKRVQLVGDDLFVTN
TKILKEGIDKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRI
SKYNQLLRIEEDLGDIASYPGKSAFYNLR
>Mature_428_residues
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIMG
LDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLD
IQEFMVMPVSATSFREALRCGAEVFHALKKILADKGMSTAVGDEGGFAPNFSSNEECLNTIVQAVEKAGYRMGEDVLLAL
DCAASEFYHEAEDVYVLEGEGLKLSSTQFADYLANLCDKFPIVSIEDGMAEGDWDGWKTLTDKLGKRVQLVGDDLFVTNT
KILKEGIDKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRIS
KYNQLLRIEEDLGDIASYPGKSAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=49.8855835240275, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.1879350348028, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=48.512585812357, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=48.512585812357, Blast_Score=404, Evalue=1e-113,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=43.9252336448598, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI169201331, Length=338, Percent_Identity=25.1479289940828, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI169201757, Length=338, Percent_Identity=25.1479289940828, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI239744207, Length=338, Percent_Identity=25.1479289940828, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=66.0421545667447, Blast_Score=562, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=49.6583143507973, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=49.6583143507973, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=199, Percent_Identity=43.21608040201, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321693, Length=439, Percent_Identity=46.2414578587699, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=47.6851851851852, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=47.6851851851852, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=47.6851851851852, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=46.5437788018433, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=50.9302325581395, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45940; Mature: 45808

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMVMPVSATSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHEECCCHHHHHHHHH
CGAEVFHALKKILADKGMSTAVGDEGGFAPNFSSNEECLNTIVQAVEKAGYRMGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHEAEDVYVLEGEGLKLSSTQFADYLANLCDKFPIVSIEDGMAEGDWDGWKT
EEHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LTDKLGKRVQLVGDDLFVTNTKILKEGIDKGIGNSILIKINQIGTLTETFAAIEMAKRAG
HHHHHCCEEEEECCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYP
CEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GKSAFYNLR
CCCCEECCC
>Mature Secondary Structure 
SAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIDLDGTENKSRLGANAMLAVSMAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCHHHHCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMVMPVSATSFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHHHEECCCHHHHHHHHH
CGAEVFHALKKILADKGMSTAVGDEGGFAPNFSSNEECLNTIVQAVEKAGYRMGEDVLLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHEAEDVYVLEGEGLKLSSTQFADYLANLCDKFPIVSIEDGMAEGDWDGWKT
EEHHHHHHHHCCCCEEEECCCCCEECHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHH
LTDKLGKRVQLVGDDLFVTNTKILKEGIDKGIGNSILIKINQIGTLTETFAAIEMAKRAG
HHHHHCCEEEEECCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYP
CEEEEECCCCCCCCCEEEEEEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
GKSAFYNLR
CCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA