Click here to switch to the map view.

The map label for this gene is pldA [C]

Identifier: 73540745

GI number: 73540745

Start: 1135177

End: 1136430

Strand: Reverse

Name: pldA [C]

Synonym: Reut_A1042

Alternate gene names: 73540745

Gene position: 1136430-1135177 (Counterclockwise)

Preceding gene: 73540746

Following gene: 73540742

Centisome position: 29.85

GC content: 64.91

Gene sequence:

>1254_bases
ATGCTCCAGACTTCAACGACCTGGGGACGGCTTTGCCTGCTGTTCGCTTGCACCTGGCCCCTCTCAGGCCACGCAGCGGT
CTCGATGCTCCAGCCTCCCCGCGTCATCGACGGCACCCGGCCACTCTCGCTCACCCTGCTGATCAGCGCGGAAACCGGCA
CCCGGCGGTACGCGGTACCTGACACGCTTGAAGTCACGGTCTCGGGCGATTTGCAGGCGCCCGTGAAGCTTGTGCTGCGG
CGCACGGAGCCAGGCCCCGCCATTCTTTCCTTGCGCAAGGGCGAAAACCGGACGATCCGCTATGCGGCGGAGCTGCCGCC
CGGGCTGCGCGGCACGGTGCGCCTGGATGCCACCGGCATCGACGCTGCACCGGTGCTCGTCACGCTGAACCAACAGGCGG
CGCCTGACCAGCCTGCCGCTGCGCCATTGGCCACCGCCCCATCCGCGCCGGCCCGTCAGATCCAGTCCGCCGAAACACCA
GCACCAATAGTCACCGTCGCCTCGGCCACCCAGCCGGCACCCGCGCCCGTCGACCTGCGCGACAGCGCGCGCCTGTCGTT
CAACGATCCGATGTACTTCATCGTCGGCGCGCACGACGGCGGCAACGCGAAGTTCCAGCTCAGCTTCAAGTACCGGATCT
TCGAAGGCACGGATCCGGCGTCGCGACGCTTGATCGACAATCTCTACTTCGGGTACACGCAGTTCTCGCTGTGGGACTTG
TCGCAGGAGTCCAAACCGTTCCGCGACACCAACTACCGACCGAGTTTCTATTACTACCTGTCGGACACCGGCGTGCATAA
CCGCGCCATCAGCCGCCTGTCGCTAGGCACCGGATTCGAGCACGAGTCCAACGGGCGCGACGGCGACCAATCACGCAGCA
TCAACACGGTCTTTCTCAAGCCGGCCTTCTATTTCGGCGACCAGAACGACTGGCACTGGCGCGTCGCGCCCAAGCTCTAC
GCCTACCTGGAAAAGAACGACAACCCCGACATCGCGCACTACCGCGGCTACATGGATCTGGAGCTGGCTTATGGCAAAGC
GGATTCGTGGGAATTCGCCGCGACCGTGCGCAAAGGCACGCGCAAGTGGTACGGCAGCGTCGACGCGCGCGTCACCTATC
CGCTCGCTCGGCTGATCCCGGGCACGGCCGGGTATCTGATGGCGGGATATTTCGTCGGCTACGGGGAAAGCCTGCTCGAC
TACAACCAGAAGGTTCCGTGGCAGTTCCGGCTCGGCTATGCGCTGTCCCGCTAA

Upstream 100 bases:

>100_bases
CCTGCGTACAATTCGTCACAAACGCTCTCGAGTGACGTGGTAGGCTTACGCGCATTGCCATCCGCCTGCGCCCGTCCAGC
CGGACAAGGCGCCTACCGAC

Downstream 100 bases:

>100_bases
CTGGCTAACCGGCCAGCGGCTTGCCTTCCGACTTCAGACGCGCATTGGCCACGGCCGCCGCAAACTGGCCATGGCCATGC
CAGCCGGTAGCCCGGCCAAG

Product: putative phospholipase

Products: NA

Alternate protein names: Phosphatidylcholine 1-acylhydrolase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLQTSTTWGRLCLLFACTWPLSGHAAVSMLQPPRVIDGTRPLSLTLLISAETGTRRYAVPDTLEVTVSGDLQAPVKLVLR
RTEPGPAILSLRKGENRTIRYAAELPPGLRGTVRLDATGIDAAPVLVTLNQQAAPDQPAAAPLATAPSAPARQIQSAETP
APIVTVASATQPAPAPVDLRDSARLSFNDPMYFIVGAHDGGNAKFQLSFKYRIFEGTDPASRRLIDNLYFGYTQFSLWDL
SQESKPFRDTNYRPSFYYYLSDTGVHNRAISRLSLGTGFEHESNGRDGDQSRSINTVFLKPAFYFGDQNDWHWRVAPKLY
AYLEKNDNPDIAHYRGYMDLELAYGKADSWEFAATVRKGTRKWYGSVDARVTYPLARLIPGTAGYLMAGYFVGYGESLLD
YNQKVPWQFRLGYALSR

Sequences:

>Translated_417_residues
MLQTSTTWGRLCLLFACTWPLSGHAAVSMLQPPRVIDGTRPLSLTLLISAETGTRRYAVPDTLEVTVSGDLQAPVKLVLR
RTEPGPAILSLRKGENRTIRYAAELPPGLRGTVRLDATGIDAAPVLVTLNQQAAPDQPAAAPLATAPSAPARQIQSAETP
APIVTVASATQPAPAPVDLRDSARLSFNDPMYFIVGAHDGGNAKFQLSFKYRIFEGTDPASRRLIDNLYFGYTQFSLWDL
SQESKPFRDTNYRPSFYYYLSDTGVHNRAISRLSLGTGFEHESNGRDGDQSRSINTVFLKPAFYFGDQNDWHWRVAPKLY
AYLEKNDNPDIAHYRGYMDLELAYGKADSWEFAATVRKGTRKWYGSVDARVTYPLARLIPGTAGYLMAGYFVGYGESLLD
YNQKVPWQFRLGYALSR
>Mature_417_residues
MLQTSTTWGRLCLLFACTWPLSGHAAVSMLQPPRVIDGTRPLSLTLLISAETGTRRYAVPDTLEVTVSGDLQAPVKLVLR
RTEPGPAILSLRKGENRTIRYAAELPPGLRGTVRLDATGIDAAPVLVTLNQQAAPDQPAAAPLATAPSAPARQIQSAETP
APIVTVASATQPAPAPVDLRDSARLSFNDPMYFIVGAHDGGNAKFQLSFKYRIFEGTDPASRRLIDNLYFGYTQFSLWDL
SQESKPFRDTNYRPSFYYYLSDTGVHNRAISRLSLGTGFEHESNGRDGDQSRSINTVFLKPAFYFGDQNDWHWRVAPKLY
AYLEKNDNPDIAHYRGYMDLELAYGKADSWEFAATVRKGTRKWYGSVDARVTYPLARLIPGTAGYLMAGYFVGYGESLLD
YNQKVPWQFRLGYALSR

Specific function: Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities [H]

COG id: COG2829

COG function: function code M; Outer membrane phospholipase A

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein. Note=One of the very few enzymes located there (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phospholipase A1 family [H]

Homologues:

Organism=Escherichia coli, GI2367300, Length=208, Percent_Identity=33.1730769230769, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003187 [H]

Pfam domain/function: PF02253 PLA1 [H]

EC number: =3.1.1.32; =3.1.1.4 [H]

Molecular weight: Translated: 46295; Mature: 46295

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTSTTWGRLCLLFACTWPLSGHAAVSMLQPPRVIDGTRPLSLTLLISAETGTRRYAVP
CCCCCCCHHHEEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEECC
DTLEVTVSGDLQAPVKLVLRRTEPGPAILSLRKGENRTIRYAAELPPGLRGTVRLDATGI
CEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC
DAAPVLVTLNQQAAPDQPAAAPLATAPSAPARQIQSAETPAPIVTVASATQPAPAPVDLR
CCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
DSARLSFNDPMYFIVGAHDGGNAKFQLSFKYRIFEGTDPASRRLIDNLYFGYTQFSLWDL
CCCEEEECCCEEEEEEECCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCEEEEEEEEC
SQESKPFRDTNYRPSFYYYLSDTGVHNRAISRLSLGTGFEHESNGRDGDQSRSINTVFLK
CCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEE
PAFYFGDQNDWHWRVAPKLYAYLEKNDNPDIAHYRGYMDLELAYGKADSWEFAATVRKGT
EEEEECCCCCEEEEECCEEEEEEECCCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHCC
RKWYGSVDARVTYPLARLIPGTAGYLMAGYFVGYGESLLDYNQKVPWQFRLGYALSR
HHHCCCCCCEEECCHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCEEEEECEEECC
>Mature Secondary Structure
MLQTSTTWGRLCLLFACTWPLSGHAAVSMLQPPRVIDGTRPLSLTLLISAETGTRRYAVP
CCCCCCCHHHEEEEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEECC
DTLEVTVSGDLQAPVKLVLRRTEPGPAILSLRKGENRTIRYAAELPPGLRGTVRLDATGI
CEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCC
DAAPVLVTLNQQAAPDQPAAAPLATAPSAPARQIQSAETPAPIVTVASATQPAPAPVDLR
CCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCC
DSARLSFNDPMYFIVGAHDGGNAKFQLSFKYRIFEGTDPASRRLIDNLYFGYTQFSLWDL
CCCEEEECCCEEEEEEECCCCCEEEEEEEEEEEEECCCHHHHHHHHHHHHCEEEEEEEEC
SQESKPFRDTNYRPSFYYYLSDTGVHNRAISRLSLGTGFEHESNGRDGDQSRSINTVFLK
CCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHEECCCCCCCCCCCCCCCCCCCEEEEEEE
PAFYFGDQNDWHWRVAPKLYAYLEKNDNPDIAHYRGYMDLELAYGKADSWEFAATVRKGT
EEEEECCCCCEEEEECCEEEEEEECCCCCCEEEECCEEEEEEEECCCCCCHHHHHHHHCC
RKWYGSVDARVTYPLARLIPGTAGYLMAGYFVGYGESLLDYNQKVPWQFRLGYALSR
HHHCCCCCCEEECCHHHHCCCCCHHHHHHHHHCCCHHHHHCCCCCCEEEEECEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]