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The map label for this gene is yfcG [H]

Identifier: 73540704

GI number: 73540704

Start: 1087782

End: 1088474

Strand: Reverse

Name: yfcG [H]

Synonym: Reut_A1001

Alternate gene names: 73540704

Gene position: 1088474-1087782 (Counterclockwise)

Preceding gene: 73540705

Following gene: 73540702

Centisome position: 28.59

GC content: 61.62

Gene sequence:

>693_bases
ATGATCGATGTCTATAGCTGGGCGACGCCCAACGGCCACAAAGTCCATATCATGCTCGAGGAGTGCGGCCTCGAGTACAA
GGTCCACCCGATCAACATCGGTGCGGGCGACCAGTTCAGCGAGGCGTTCCTCAAGATCAGCCCGAACAACAAGATCCCGG
CCATCGTTGACCCTGACGGTCCGGACGGCGAGCCGATCTCGCTGTTTGAATCCGGCGCCATCCTGCTTTACCTGGCCGGC
AAGACCGGCAAGTTCCTGCCGGAAGACGTGCGCGGCAAGTACGAGGCGCTGCAATGGCTGATGTTCCAGATGGGCGGCGT
TGGCCCGATGCTTGGCCAGGCCCACCACTTCCGCATCTACGCGCCCGAGAAAATCGAGTATGCGGTCAATCGCTACACCA
ACGAAGCGAAGCGCCTGTACGGCGTGATCGACACGCAGCTGTCGCGGCATGAATGGCTGGCCGGCGACCAGTACACGATC
GCCGATATCGCCACGTTCCCGTGGCTGCGCAGTTGGCACAACCAGGGCGTGGAACTCGACGAGTATCCGAACCTCAAGCG
CTGGTTCAACACCATCGCAGAACGCCCGGCCGTGAAGCGCGGCGTCGAAGTTCTGGCCAGCGCGCGCAAGCCCCTGCAGG
ACGACAAGGCTCGCGAAATTCTATTCGGCGCCACGCAATACAAGCGTCACTGA

Upstream 100 bases:

>100_bases
AAACGCGCAGCCCCGCTGCGCGTTTTTTTCTTCTGTGCTTAAATCGATCGATCCTTTTCCGCCCCTCCTTCCCCATATCG
CCGTCAGGAGACTTCTTGCG

Downstream 100 bases:

>100_bases
AGCCGGATGAACGCCGGCGGCATGCATTGCCGCCGGTTGATTGCGCCCTGCTTCAGGCCGGCAGGCACTCCGCTTGCGCG
CCGTACGGCGCGCGCGGCAT

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MIDVYSWATPNGHKVHIMLEECGLEYKVHPINIGAGDQFSEAFLKISPNNKIPAIVDPDGPDGEPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDTQLSRHEWLAGDQYTI
ADIATFPWLRSWHNQGVELDEYPNLKRWFNTIAERPAVKRGVEVLASARKPLQDDKAREILFGATQYKRH

Sequences:

>Translated_230_residues
MIDVYSWATPNGHKVHIMLEECGLEYKVHPINIGAGDQFSEAFLKISPNNKIPAIVDPDGPDGEPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDTQLSRHEWLAGDQYTI
ADIATFPWLRSWHNQGVELDEYPNLKRWFNTIAERPAVKRGVEVLASARKPLQDDKAREILFGATQYKRH
>Mature_230_residues
MIDVYSWATPNGHKVHIMLEECGLEYKVHPINIGAGDQFSEAFLKISPNNKIPAIVDPDGPDGEPISLFESGAILLYLAG
KTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIYAPEKIEYAVNRYTNEAKRLYGVIDTQLSRHEWLAGDQYTI
ADIATFPWLRSWHNQGVELDEYPNLKRWFNTIAERPAVKRGVEVLASARKPLQDDKAREILFGATQYKRH

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=52.7093596059113, Blast_Score=221, Evalue=4e-59,
Organism=Escherichia coli, GI87082195, Length=227, Percent_Identity=47.136563876652, Blast_Score=187, Evalue=5e-49,
Organism=Escherichia coli, GI87081789, Length=173, Percent_Identity=29.4797687861272, Blast_Score=83, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324100, Length=234, Percent_Identity=34.1880341880342, Blast_Score=128, Evalue=8e-31,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933730, Length=222, Percent_Identity=27.9279279279279, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17864592, Length=198, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17864598, Length=175, Percent_Identity=30.2857142857143, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI85725204, Length=201, Percent_Identity=28.3582089552239, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17737923, Length=201, Percent_Identity=28.3582089552239, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17864594, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646249, Length=179, Percent_Identity=27.9329608938547, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI20130115, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26170; Mature: 26170

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKVHIMLEECGLEYKVHPINIGAGDQFSEAFLKISPNNKIPAIVDPDG
CCCEEECCCCCCCEEEEEEHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCC
PDGEPISLFESGAILLYLAGKTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIEYAVNRYTNEAKRLYGVIDTQLSRHEWLAGDQYTIADIATFPWLRSWHNQGVELD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
EYPNLKRWFNTIAERPAVKRGVEVLASARKPLQDDKAREILFGATQYKRH
CCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHCCC
>Mature Secondary Structure
MIDVYSWATPNGHKVHIMLEECGLEYKVHPINIGAGDQFSEAFLKISPNNKIPAIVDPDG
CCCEEECCCCCCCEEEEEEHHCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCEEECCCC
PDGEPISLFESGAILLYLAGKTGKFLPEDVRGKYEALQWLMFQMGGVGPMLGQAHHFRIY
CCCCCEEEECCCEEEEEEECCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCEEEEE
APEKIEYAVNRYTNEAKRLYGVIDTQLSRHEWLAGDQYTIADIATFPWLRSWHNQGVELD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCC
EYPNLKRWFNTIAERPAVKRGVEVLASARKPLQDDKAREILFGATQYKRH
CCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]