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The map label for this gene is ureF

Identifier: 73540700

GI number: 73540700

Start: 1084899

End: 1085591

Strand: Direct

Name: ureF

Synonym: Reut_A0997

Alternate gene names: 73540700

Gene position: 1084899-1085591 (Clockwise)

Preceding gene: 73540699

Following gene: 73540701

Centisome position: 28.5

GC content: 62.63

Gene sequence:

>693_bases
ATGACACCGCTGCGTCAGCTCATCTCCCTCCTGCACCTGGCCTCACCCGCGCTCCCAATCGGTGGCTTCAGCTATTCGCA
AGGTCTCGAAGCCGCCATCGACATTGGCTGTGTCCGTGATGCAGATACCGCCGAACGCTGGATTCGCGACAGTCTGCTAT
ACGTCCAGGCGCAATGCGAAGCGCCGTTGTGGCTGCTGCTGCATCGCGGCTGGCAGGCGATGGATGTCGATGCGGTGCGC
GAGTGGAACGGCTGGTTCCATGCCACCCGCGAAACGGCCGAGTTGCGGCTCGAGACCGAACAGATGGGCTGGTCGCTCGC
GAAGCTCGTGGGGCAGATGGGATGGGGTGGCGATGCGTTGCGCGAGCATCTTGCCGTCATATCGCCGGTATGCCTGCCGA
CGGCGTTTGCGGCAACCTGCGTGGCGCTGGAGATCGATGAGCGCGAGGGGCTGGCCGCTTACTGCTTCAACTGGGCGGAA
AACCAAGTGGCTGCCGCTATCAAGGCCGTGCCGCTAGGGCAGGTGGCCGGCCAGCACATGCTGCGGCGTCTGCACGAAGC
CGTGCTGATGACAGTTGATGAAGCCGTACAACGCGCCGCGGAAACGCCACCAAGGCTTTCCACCTTTTCACCAATGCTGG
GATTGCTTTCGTCGCGTCACGAAACGCAATATTCCCGGCTGTTCCGTTCCTGA

Upstream 100 bases:

>100_bases
CGATCACGATCACGATCACGATCACGAGCATGATGTAAAGGGGCACGTTCACGGCCCCGGCTGCGGGCACAAGCACTGAA
CGCGTAGCACACCGCCGGCC

Downstream 100 bases:

>100_bases
ATCCCGAAGCGATCATGACCCAGGCTCGTACCAAAAAGAATCCTCCGCTGCGCGTCGGCGTCGGCGGACCTGTCGGCTCC
GGCAAGACCACTCTGCTGGA

Product: urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTPLRQLISLLHLASPALPIGGFSYSQGLEAAIDIGCVRDADTAERWIRDSLLYVQAQCEAPLWLLLHRGWQAMDVDAVR
EWNGWFHATRETAELRLETEQMGWSLAKLVGQMGWGGDALREHLAVISPVCLPTAFAATCVALEIDEREGLAAYCFNWAE
NQVAAAIKAVPLGQVAGQHMLRRLHEAVLMTVDEAVQRAAETPPRLSTFSPMLGLLSSRHETQYSRLFRS

Sequences:

>Translated_230_residues
MTPLRQLISLLHLASPALPIGGFSYSQGLEAAIDIGCVRDADTAERWIRDSLLYVQAQCEAPLWLLLHRGWQAMDVDAVR
EWNGWFHATRETAELRLETEQMGWSLAKLVGQMGWGGDALREHLAVISPVCLPTAFAATCVALEIDEREGLAAYCFNWAE
NQVAAAIKAVPLGQVAGQHMLRRLHEAVLMTVDEAVQRAAETPPRLSTFSPMLGLLSSRHETQYSRLFRS
>Mature_229_residues
TPLRQLISLLHLASPALPIGGFSYSQGLEAAIDIGCVRDADTAERWIRDSLLYVQAQCEAPLWLLLHRGWQAMDVDAVRE
WNGWFHATRETAELRLETEQMGWSLAKLVGQMGWGGDALREHLAVISPVCLPTAFAATCVALEIDEREGLAAYCFNWAEN
QVAAAIKAVPLGQVAGQHMLRRLHEAVLMTVDEAVQRAAETPPRLSTFSPMLGLLSSRHETQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_CUPPJ (Q473Q7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295220.1
- GeneID:   3609198
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0997
- NMPDR:   fig|264198.3.peg.1510
- HOGENOM:   HBG709295
- OMA:   GFAWLEG
- ProtClustDB:   CLSK897184
- BioCyc:   REUT264198:REUT_A0997-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 25536; Mature: 25404

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLRQLISLLHLASPALPIGGFSYSQGLEAAIDIGCVRDADTAERWIRDSLLYVQAQCE
CCHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHEEECCC
APLWLLLHRGWQAMDVDAVREWNGWFHATRETAELRLETEQMGWSLAKLVGQMGWGGDAL
CHHHHHHHCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
REHLAVISPVCLPTAFAATCVALEIDEREGLAAYCFNWAENQVAAAIKAVPLGQVAGQHM
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LRRLHEAVLMTVDEAVQRAAETPPRLSTFSPMLGLLSSRHETQYSRLFRS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TPLRQLISLLHLASPALPIGGFSYSQGLEAAIDIGCVRDADTAERWIRDSLLYVQAQCE
CHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHEEECCC
APLWLLLHRGWQAMDVDAVREWNGWFHATRETAELRLETEQMGWSLAKLVGQMGWGGDAL
CHHHHHHHCCCCCCCHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
REHLAVISPVCLPTAFAATCVALEIDEREGLAAYCFNWAENQVAAAIKAVPLGQVAGQHM
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LRRLHEAVLMTVDEAVQRAAETPPRLSTFSPMLGLLSSRHETQYSRLFRS
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA