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The map label for this gene is pnp

Identifier: 73540660

GI number: 73540660

Start: 1044324

End: 1046510

Strand: Direct

Name: pnp

Synonym: Reut_A0957

Alternate gene names: 73540660

Gene position: 1044324-1046510 (Clockwise)

Preceding gene: 73540659

Following gene: 73540661

Centisome position: 27.44

GC content: 64.61

Gene sequence:

>2187_bases
ATGTCCATGTTCAACAAGGTTGTGAAGCAGTTCCAGTGGGGCCAGCATACGGTCCGCATGGAAACCGGCGAAATCGCCCG
CCAGGCCGGCGGCGCCGTGATCGTCGACGTGGAAGATACCGTCGTGCTGGCGACTGTCGTCGCCGCCAAGAGCCCGAAGC
CCGGCCAGGACTTCTTCCCGCTGACCGTCGACTACATCGAAAAGACCTACGCTGCCGGCAAGATCCCCGGTGGCTTCTTC
AAGCGCGAAGGCCGTCCGTCGGAAAACGAGACGCTGACTTCGCGCCTGATCGACCGTCCGCTGCGCCCGCTGTTCCCGGA
AGGCTTCTACAACGACGTGCAGGTGGTCGTGCACGTGGTGTCGCTGAATCCGGAAATCCCGGCTGACATCCCTGCGCTGA
TCGGCGCGTCCGCCGCGCTGGCCGTGTCGGGCATCCCGTTCAACGGCCCGGTGGGCGCTGCACGTATCGGCTACAAGGAC
GGCCAGTACCTGCTGAACCCGACCCGTTCGCAAATGGCCACGTCGGACCTCGACCTCGTCGTCGCTGGTACCGAGCGCGC
CGTGCTGATGGTGGAATCGGAAGCCAACCAGCTGTCCGAAGACGTGATGCTGGGCGCCGTGGTGTATGGCCACGAGCAGA
TGCAGATCGCCATCAACGCGATCCATGATCTCGTGCGCGAAGGCGGCAAGCCCGAGTGGGACTGGGCGCCTGCGCCGAAG
AACGAAGCGCTGATCGCCAAGGTCACCGAAGTCGCGCTGCCGCTGCTGCAGGAAGCCTATCAGCTGCGCCAGAAGTCGGC
CCGCAGCCAGAAGCTGAAGGAAGTGTCGGCGAACGTGACGGCCGCCCTGGCCGAGGCCGGCGTCGACGCTGACAAGGTGG
AAGTCGGCAACATCATGTTCGACCTCGAAGCGAAGATCGTCCGCGGACAGATCCTCGGCGGCGAGCCGCGCATCGACGGC
CGCGACACCCGCACGGTGCGTCCGATCGAGATCCGTTCGTCGGTCCTGCCGCGCGCCCACGGCTCGGCCCTGTTCACCCG
CGGTGAAACCCAGGCGCTGGTCGTGGCCACGCTGGGCACGAAGAGCGACGAGCAGATCATCGACGCGCTGGCCGGTGAAT
ACCGCGACCGCTTCATGCTCCACTACAACATGCCTCCGTTCGCCACCGGCGAAACCGGCCGCGTCGGCAGCCCGAAGCGC
CGCGAAATCGGCCATGGCCGCCTGGCCAAGCGCGCACTGATTCCGGTGCTGCCGAAGGACGACGAGTTCGCCTACACCAT
CCGCCTGGTTTCGGAAATCACCGAATCGAACGGTTCGTCGTCGATGGCTTCGGTCTGCGGCGGCTGCCTGGCGCTGATGG
ACGCCGGCGTTCCGGTCAAGGCGCACGTGGCCGGCGTGGCCATGGGTCTGATCCTGGAAGGCAACAAGTTTGCCGTGCTG
ACCGACATCCTCGGTGATGAAGATCACCTGGGCGACATGGACTTCAAGGTGGCGGGTACCGACAACGGCATCACCGCGCT
GCAGATGGACATCAAGGTGCAGGGCATCACCAAGGAAATCATGCAGGTTGCGCTGGCGCAAGCCAAGGAAGGCCGTCTGC
ATATCCTGGGCGCCATGCAGGGTGCCATGGGCCACGCGCGCACCGAGCTGTCGGCGCACGCACCGCGCATGATCACCATG
AAGATCCATCCGGACAAGATCCGCGAAGTGATCGGCAAGGGTGGCTCGACCATCCAGGCGCTGACCAAGGAAACCGGCAC
GACCATCGACATCCAGGAAGACGGCACGATCACCATCGCGTCGACCTCGACCGATGGCATGGCAGAAGCCAAGCGCCGTA
TCGAGGGCATTACCGCCGAAGCCGAAGTGGGCAAGATCTACAACGGCACGGTGCTGAAGCTGCTGGACTTCGGCGCGATC
GTGAACATCCTGCCGGGCAAGGATGGTCTGCTGCATATCTCCGAGATCGTCAACGAGCGCGTGAAGGACATCAAGGACTG
GCTGAAGGAAGGCCAGCAACTGCGCGTCAAGCTGATCCAGGCTGACGAGAAGGGCCGTCTGCGCCTGTCGCTGAAGGCTG
CGCTGGCGGAAGAGGGTGGCAGCATCAGCCCGGTGAACGCTGGTGAAGCGGCTCCGGCTCCGGCTCCGGCTGCGGCTCCG
GCAACTCCGTCGGACCAGCAGCAATAA

Upstream 100 bases:

>100_bases
CTGCAACTGCGACCGCATCCGGCAGTTAGCCCGCGGCTGAGAACGCGGGCGAGACACGCCGCGCAACGATTGCGGCGTGC
GCGCAAGACAAGGAATGCAA

Downstream 100 bases:

>100_bases
GCTGCAATCGGTGCCGCCTCAGGCGGCGTCGGACAAAAAACGGCTGGCGATGTCCAGCCGTTTTTTTGTTTACACTGCCG
GGCAACCCATTGAGCAATCG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 728; Mature: 727

Protein sequence:

>728_residues
MSMFNKVVKQFQWGQHTVRMETGEIARQAGGAVIVDVEDTVVLATVVAAKSPKPGQDFFPLTVDYIEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVVHVVSLNPEIPADIPALIGASAALAVSGIPFNGPVGAARIGYKD
GQYLLNPTRSQMATSDLDLVVAGTERAVLMVESEANQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
NEALIAKVTEVALPLLQEAYQLRQKSARSQKLKEVSANVTAALAEAGVDADKVEVGNIMFDLEAKIVRGQILGGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
TDILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGAMQGAMGHARTELSAHAPRMITM
KIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAI
VNILPGKDGLLHISEIVNERVKDIKDWLKEGQQLRVKLIQADEKGRLRLSLKAALAEEGGSISPVNAGEAAPAPAPAAAP
ATPSDQQQ

Sequences:

>Translated_728_residues
MSMFNKVVKQFQWGQHTVRMETGEIARQAGGAVIVDVEDTVVLATVVAAKSPKPGQDFFPLTVDYIEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVVHVVSLNPEIPADIPALIGASAALAVSGIPFNGPVGAARIGYKD
GQYLLNPTRSQMATSDLDLVVAGTERAVLMVESEANQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
NEALIAKVTEVALPLLQEAYQLRQKSARSQKLKEVSANVTAALAEAGVDADKVEVGNIMFDLEAKIVRGQILGGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
TDILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGAMQGAMGHARTELSAHAPRMITM
KIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAI
VNILPGKDGLLHISEIVNERVKDIKDWLKEGQQLRVKLIQADEKGRLRLSLKAALAEEGGSISPVNAGEAAPAPAPAAAP
ATPSDQQQ
>Mature_727_residues
SMFNKVVKQFQWGQHTVRMETGEIARQAGGAVIVDVEDTVVLATVVAAKSPKPGQDFFPLTVDYIEKTYAAGKIPGGFFK
REGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVVHVVSLNPEIPADIPALIGASAALAVSGIPFNGPVGAARIGYKDG
QYLLNPTRSQMATSDLDLVVAGTERAVLMVESEANQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPKN
EALIAKVTEVALPLLQEAYQLRQKSARSQKLKEVSANVTAALAEAGVDADKVEVGNIMFDLEAKIVRGQILGGEPRIDGR
DTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGTKSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRR
EIGHGRLAKRALIPVLPKDDEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVLT
DILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGAMQGAMGHARTELSAHAPRMITMK
IHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIASTSTDGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIV
NILPGKDGLLHISEIVNERVKDIKDWLKEGQQLRVKLIQADEKGRLRLSLKAALAEEGGSISPVNAGEAAPAPAPAAAPA
TPSDQQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=704, Percent_Identity=38.7784090909091, Blast_Score=457, Evalue=1e-128,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=61.7052023121387, Blast_Score=894, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=707, Percent_Identity=32.2489391796322, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=37.8223495702006, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=37.8223495702006, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=37.8223495702006, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=649, Percent_Identity=37.2881355932203, Blast_Score=397, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_CUPPJ (Q473U7)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_295180.1
- HSSP:   O29756
- ProteinModelPortal:   Q473U7
- GeneID:   3609754
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A0957
- NMPDR:   fig|264198.3.peg.1357
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   REUT264198:REUT_A0957-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77991; Mature: 77860

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNKVVKQFQWGQHTVRMETGEIARQAGGAVIVDVEDTVVLATVVAAKSPKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVVHVV
CCHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEE
SLNPEIPADIPALIGASAALAVSGIPFNGPVGAARIGYKDGQYLLNPTRSQMATSDLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEECCCCCCEEECCCHHHHHCCCCEEE
VAGTERAVLMVESEANQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
EECCCEEEEEEECCHHHHHHHHHEEHEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIAKVTEVALPLLQEAYQLRQKSARSQKLKEVSANVTAALAEAGVDADKVEVGNIMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEE
DLEAKIVRGQILGGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGT
EEEEEEEEEEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECC
KSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKD
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
DEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGAMQ
EECCCCCCCCCCCEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEHH
GAMGHARTELSAHAPRMITMKIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCEEEEE
STSTDGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIVNILPGKDGLLHISEIVNER
ECCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCHHEEECCCCCCCCHHHHHHHHH
VKDIKDWLKEGQQLRVKLIQADEKGRLRLSLKAALAEEGGSISPVNAGEAAPAPAPAAAP
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
ATPSDQQQ
CCCCCCCC
>Mature Secondary Structure 
SMFNKVVKQFQWGQHTVRMETGEIARQAGGAVIVDVEDTVVLATVVAAKSPKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEHHHHCCCCCCCCCCCC
LTVDYIEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNDVQVVVHVV
CCHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEEE
SLNPEIPADIPALIGASAALAVSGIPFNGPVGAARIGYKDGQYLLNPTRSQMATSDLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEECCCCCCEEECCCHHHHHCCCCEEE
VAGTERAVLMVESEANQLSEDVMLGAVVYGHEQMQIAINAIHDLVREGGKPEWDWAPAPK
EECCCEEEEEEECCHHHHHHHHHEEHEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIAKVTEVALPLLQEAYQLRQKSARSQKLKEVSANVTAALAEAGVDADKVEVGNIMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEE
DLEAKIVRGQILGGEPRIDGRDTRTVRPIEIRSSVLPRAHGSALFTRGETQALVVATLGT
EEEEEEEEEEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECC
KSDEQIIDALAGEYRDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIPVLPKD
CCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCCCCCC
DEFAYTIRLVSEITESNGSSSMASVCGGCLALMDAGVPVKAHVAGVAMGLILEGNKFAVL
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDNGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGAMQ
EECCCCCCCCCCCEEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEHH
GAMGHARTELSAHAPRMITMKIHPDKIREVIGKGGSTIQALTKETGTTIDIQEDGTITIA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCEEEEE
STSTDGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIVNILPGKDGLLHISEIVNER
ECCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCHHEEECCCCCCCCHHHHHHHHH
VKDIKDWLKEGQQLRVKLIQADEKGRLRLSLKAALAEEGGSISPVNAGEAAPAPAPAAAP
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
ATPSDQQQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA